Article
Polymer Science
J. Lucas Vallejo-Garcia, Ana Arnaiz, Maria D. Busto, Jose M. Garcia, Safil Vallejos
Summary: This study presents the synthesis and characterization of a polyacrylic film material for enzyme immobilization. The material achieves efficient immobilization of the enzyme through the formation of azo linkages and demonstrates superior performance in lactose hydrolysis.
EUROPEAN POLYMER JOURNAL
(2023)
Article
Chemistry, Physical
Sami A. Al-Harbi, Yaaser Q. Almulaiky
Summary: The immobilization of beta-galactosidase on Cu-trimesic acid-based metal organic frameworks (Cu-TMAs) was studied. The immobilized enzyme showed good retention of activity, storage stability, and improved lactose hydrolysis activity. This study demonstrated the potential of Cu-TMAs as supports for immobilizing beta-galactosidase and suggested practical applications in various industrial processes.
JOURNAL OF MOLECULAR LIQUIDS
(2023)
Review
Biotechnology & Applied Microbiology
Feiyu Duan, Tong Sun, Jingwen Zhang, Ke Wang, Yan Wen, Lili Lu
Summary: Immobilized enzymes, especially ll-galactosidases, have been improved through innovative methods like rational adsorption, layer by layer adsorption, 3-D printing, and covalent binding, leading to enhanced performance and broader industrial applications in food, energy, and medicine.
BIOTECHNOLOGY ADVANCES
(2022)
Review
Food Science & Technology
Patrick Schulz, Syed S. H. Rizvi
Summary: The lactose-free dairy market has grown significantly in recent years, with lactose hydrolysis being the most commonly used method due to its economic and sustainable advantages. Current research mainly focuses on enzymatic hydrolysis methods and developing new commercially valuable products.
FOOD REVIEWS INTERNATIONAL
(2023)
Article
Agriculture, Multidisciplinary
Belay Tilahun Tadesse, Ge Zhao, Paul Kempen, Christian Solem
Summary: This study discovered that a mutated strain of Streptococcus thermophilus can efficiently hydrolyze lactose using whole-cell lactase catalysts. Genome sequencing revealed a mutation that affects the growth of the strain and increases its lactose hydrolyzing ability. These findings could potentially lead to the production of sweet lactose-reduced yoghurt without the need for expensive purified lactase enzymes.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Food Science & Technology
Shuyue Luan, Xuguo Duan
Summary: In this study, a novel thermostable β-galactosidase (lacZBa) from Bacillus aryabhattai GEL-09 was investigated and found to have potential applications in the dairy industry. LacZBa exhibited maximum activity at 45°C and pH 6.0, and could hydrolyze lactose and oNPG. It efficiently recognized and catalyzed lactose, and could completely hydrolyze lactose in lactose solution and commercial milk within 4 hours at 45°C.
Article
Biochemistry & Molecular Biology
Leszek Kadzinski, Robert Lyzen, Katarzyna Bury, Bogdan Banecki
Summary: The studies demonstrate that by optimizing the sol-gel process parameters and adding polydimethylsiloxane, the activity and thermal stability of beta-galactosidase can be enhanced, which is of great significance for industrial applications such as lactose elimination.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Yang Liu, Zufang Wu, Xiaoxiong Zeng, Peifang Weng, Xin Zhang, Changyu Wang
Summary: BsGal1332 is a cold-adapted enzyme with strict substrate specificity for galactosyl compounds, making it suitable for efficient lactose hydrolysis in dairy products.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Chemistry, Multidisciplinary
Duana Ceciliane Hanauer, Alana Gabrieli de Souza, Mariana Aguiar Cargnin, Bruna Carla Gasparin, Derval dos Santos Rosa, Alexandre Tadeu Paulino
Summary: In this study, pectin-based biohydrogels were synthesized with or without eucalyptus sawdust, and used for immobilizing beta-D-galactosidase for lactose hydrolysis reactions. The addition of eucalyptus sawdust increased the thermal and mechanical resistance of the biomaterial, preserving enzyme stability. The immobilized enzyme in pectin-based biohydrogels showed potential for producing lactose-free or low-dosage lactose foods.
JOURNAL OF INDUSTRIAL AND ENGINEERING CHEMISTRY
(2021)
Article
Chemistry, Applied
Payal Singh, Sumit Arora, Priyanka Singh Rao, Deepika Kathuria, Vivek Sharma, Ashish Kumar Singh
Summary: This study evaluated the activity of β-galactosidase for lactose hydrolysis and galactooligosaccharide formation at 7 degrees C. The enzyme derived from K. lactis was more effective than B. lichenformis. When used in concentrated skim milk, the enzyme concentration of 9.53 U/mL resulted in the highest hydrolysis rate and the maximum formation of galactooligosaccharides was obtained after 4 hours.
Article
Food Science & Technology
Selen Kizildag, Ceyhun Isik, Mustafa Teke
Summary: In this study, beta-galactosidase was successfully immobilized on eggshell membrane (ESM) using adsorption and cross-linking method. The interaction between enzyme and ESM was analyzed using ATR-FTIR and SEM. The immobilized enzyme showed improved stability and could retain more than 50% of its activity after multiple reuses. The highest lactose removal efficiency of 55.8% was achieved by using beta-galactosidase immobilized ESM under optimized conditions.
EUROPEAN FOOD RESEARCH AND TECHNOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Yasmin R. Maghraby, Rehan M. El-Shabasy, Ahmed H. Ibrahim, Hassan Mohamed El-Said Azzazy
Summary: Enzymes are crucial in various industries and are essential for the production of industrial products. Immobilized enzymes are more resistant to environmental changes and can be easily recovered/recycled compared to free enzymes. Immobilization protects enzymes from harsh conditions and enables their use in large-scale industries such as medical, food, detergent, textile, pharmaceutical, and water treatment. Different techniques, including covalent binding, encapsulation, entrapment, and adsorption, are employed for enzyme immobilization. This review discusses enzyme immobilization techniques, their industrial applications from 1992 to 2022, and the impact of the COVID-19 pandemic on global enzyme production.
Article
Chemistry, Multidisciplinary
Defne I. Dayi, Ursula Eschenhagen, Henrike Seidinger, Holger Schneider, Magnus S. Schmidt
Summary: In this study, we demonstrated the immobilization of a technical-grade & beta;-d-galactosidase on amino-functionalized microtiter plates and transferred the results to a resin-based approach. The enzyme was covalently bound to an amino-functionalized microtiter plate prefunctionalized with 1,4-phenylendiisothiocyanate. The cleavage of the substrate 5-bromo-4-chloro-3-indoxyl-& beta;-d-galactopyranoside (X-Gal) produced a deep blue dye, which was quantified in a microtiter plate reader at 595 nm. The maximum reaction rates and the Michaelis-Menten constant were calculated, and optimization of the experiments minimized the formation of unwanted blue precipitate.
Review
Chemistry, Multidisciplinary
Rahul Vikram Singh, Krishika Sambyal
Summary: This review provides systematic information about the recent advancements in the production of beta-galactosidase and its industrial applications, primarily focusing on the application of recombinant strains and immobilization strategies. These developments have made beta-galactosidase an important enzyme in the industry.
Article
Biochemistry & Molecular Biology
Isabel Fraile-Gutierrez, Susana Iglesias, Niuris Acosta, Julia Revuelta
Summary: This research developed a new hydrogel that can protect beta-galactosidase from degradation in the stomach and retain its activity in the intestine. It represents a promising approach for oral transport of the enzyme to reduce the symptoms of hypolactasia.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Chemistry, Inorganic & Nuclear
Navneet Sharma, Rita Kakkar, Prerna Bansal, Anju Singh, Himanshu Ojha, Dharam Pal Pathak, Rakesh Kumar Sharma
INORGANICA CHIMICA ACTA
(2019)
Article
Materials Science, Biomaterials
Navneet Sharma, Mamta Chaudhary, Bhupendra Singh Butola, Joseph Kingston Jeyabalaji, Dharm Pal Pathak, Rakesh Kumar Sharma
MATERIALS SCIENCE AND ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
(2019)
Article
Pharmacology & Pharmacy
Sarwar Beg, Shashi Dhiman, Teenu Sharma, Ashish Jain, Rakesh Kumar Sharma, Atul Jain, Bhupinder Singh
Article
Pharmacology & Pharmacy
Manvi Singh, Nazia Hassan, Devina Verma, Pragya Thakur, Bibhu Prasad Panda, Amulya Kumar Panda, Rakesh Kumar Sharma, Aamir Mirza, Sheikh Mansoor, Salman H. Alrokayan, Haseeb A. Khan, Parvaiz Ahmad, Zeenat Iqbal
SAUDI PHARMACEUTICAL JOURNAL
(2020)
Review
Biology
Deepak Kaushik, Pooja Jangra, Ravinder Verma, Deepika Purohit, Parijat Pandey, Sandeep Sharma, Rakesh Kumar Sharma
Summary: The article discusses the importance and development trends of radioisotopes and radiopharmaceuticals in modern healthcare, emphasizing safety and effectiveness issues in regulation, as well as the challenges and uncertainties that exist in the field.
JOURNAL OF BIOSCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Ashrit Nair, Pooja Yadav, Amanpreet Behl, Rakesh Kumar Sharma, Shweta Kulshrestha, Bhupendra Singh Butola, Navneet Sharma
Summary: Blister agents have been a major threat to individuals since the First World War, causing significant casualties. Understanding their chemistry and toxicological mechanisms is crucial for developing effective decontamination and therapeutic strategies. While decontamination solutions and detection technologies have been developed, there is still a lack of proper understanding of the toxicological mechanism of action of these compounds.
CHEMICO-BIOLOGICAL INTERACTIONS
(2021)
Review
Infectious Diseases
Amanpreet Behl, Ashrit Nair, Sanika Mohagaonkar, Pooja Yadav, Kirtida Gambhir, Nishant Tyagi, Rakesh Kumar Sharma, Bhupendra Singh Butola, Navneet Sharma
Summary: For centuries, the world has faced numerous infectious diseases, some of which have the potential to become pandemics and pose a constant threat to human lives. Understanding the pathogens and the progression of these diseases is crucial for their timely control. This study provides a chronology of past pandemics, an evolutionary analysis of infectious diseases with pandemic potential, and identifies likely pathogens that could trigger future pandemics. By studying the evolutionary history and similarity between different infectious diseases, this research aims to aid in the development of early detection techniques, preventive measures, infection control, and therapeutic options to strengthen global preparedness against future pandemics.
INFECTION GENETICS AND EVOLUTION
(2022)
Article
Pharmacology & Pharmacy
Sabna Kotta, Abdul Wadood Khan, Shahid H. Ansari, Rakesh Kumar Sharma, Y. T. Kamal, Hibah Mubarak Aldawsari, Nabil Abdulhafiz Alhakamy, Sanjula Baboota, Javed Ali
Summary: The development of a nanoemulsion formulation of efavirenz has shown promising results in improving drug permeability and bioavailability, addressing the challenges of inaccurate dosing in pediatric antiretroviral therapy for HIV infection. The formulation has demonstrated an effective dose adjustable formulation for HIV therapy, especially in pediatric population.
INTERNATIONAL JOURNAL OF PHARMACOLOGY
(2022)
Review
Pharmacology & Pharmacy
Sandeep Sharma, Rakesh Kumar Sharma, Rajesh Kumar Singh
Summary: This article focuses on the Mayapuri radiological incident in Delhi and emphasizes the need for improved management of radiation incidents and enhanced medical preparedness measures to prevent and respond to radiation casualties.
CURRENT RADIOPHARMACEUTICALS
(2022)
Article
Environmental Sciences
Chitvan Pandit, Hema Kumari Alajangi, Joga Singh, Akhil Khajuria, Akanksha Sharma, Md. Samim Hassan, Manmohan Parida, Anil Dutt Semwal, Natarajan Gopalan, Rakesh Kumar Sharma, Ashish Suttee, Udit Soni, Bhupinder Singh, Sameer Sapra, Ravi Pratap Barnwal, Gurpal Singh, Indu Pal Kaur
Summary: In this study, a prototype biosensor using magnetic nanoparticles and fluorescent probes was developed for rapid and accurate detection of Escherichia coli in water and food samples.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Pharmacology & Pharmacy
Chintankumar V. Patel, Amit Tyagi, Rakesh Kumar Sharma, Hetal P. Thakkar
Summary: The study aimed to investigate the uterine targeting potential of Paclitaxel (PTX) loaded ultradeformable vesicles (PTX-UDV) after intra-vaginal administration. PTX-UDV were formulated and characterized, and the radiolabeled PTX (99mTc-PTX) was evaluated for its stability and binding efficiency. The biodistribution of 99mTc-PTX loaded UDV and plain 99mTc-PTX was assessed in rabbits after intra-vaginal administration.
JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
(2023)
Review
Radiology, Nuclear Medicine & Medical Imaging
Prasad Thota, Anusha Thota, Manoj Kumar Pandey, Phulen Sarma, Bikash Medhi, Rakesh Kumar Sharma, Jai Prakash, Anil Kumar Teotia
Summary: The Indian Pharmacopoeia (IP) has included monographs for radiopharmaceutical preparations for the first time in 2014, with standards regularly updated and applicable in various laboratories in India and abroad. This review provides an overview of the journey of setting standards for radiopharmaceuticals in IP, along with a comparative analysis of regulatory perspectives in different jurisdictions.
INDIAN JOURNAL OF NUCLEAR MEDICINE
(2021)
Article
Pharmacology & Pharmacy
D. Uma Maheswari, T. Anand, T. Mohan Manu, Farhath Khanum, Rakesh Kumar Sharma
INTERNATIONAL JOURNAL OF PHARMACEUTICAL SCIENCES AND RESEARCH
(2019)
Review
Multidisciplinary Sciences
Sandeep Sharma, Sanyog Jain, Ashish Baldi, Rajesh K. Singh, Rakesh Kumar Sharma