Article
Fisheries
Nantawut Ponpukdee, Pradit Wangman, Channarong Rodkhum, Chalinan Pengsuk, Parin Chaivisuthangkura, Paisarn Sithigorngul, Siwaporn Longyant
Summary: Monoclonal antibodies generated from outer membrane proteins of pathogenic Flavobacterium columnare can be used as a simple, fast, specific, and low-cost immunological tool for the direct detection of F. columnare without the need for bacterial isolation as in conventional biochemical tests or DNA extraction as in PCR assays. By combining specific MAbs, the detection limit in dot blotting assay can be increased to 5 x 10(2) cfu/dot.
Article
Fisheries
Zhao Jia, Jianhua Feng, Gaoliang Yuan, Hehe Xiao, Huifeng Dang, Yanwei Zhang, Kangyong Chen, Jun Zou, Junya Wang
Summary: Monoclonal antibodies against grass carp Metrnl-a were prepared and characterized in this study. The expression of CiMetrnl-a increased in grass carp infected with A. hydrophila, particularly in the gills, head kidney and intestine. CiMetrnl-a producing cells were mainly detected in the mucosal layer of the inflamed intestine caused by A. hydrophila infection. qRT-PCR analysis confirmed the marked induction of CiMetrnl-a mRNA expression. These findings suggest the involvement of CiMetrnl-a in regulating bacterial infection-induced intestine inflammation.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2023)
Article
Microbiology
Yang Wang, Yunlu Wei, Nan Shang, Pinglan Li
Summary: This study showed that the combination of PlnEF and lactic acid had synergistic antimicrobial activity against the Gram-negative pathogen Aeromonas hydrophila. Lactic acid facilitated the interaction between PlnEF and the inner cell membrane of A. hydrophila by inducing the release of lipopolysaccharide (LPS). The combined treatment caused severe morphological and intracellular changes in A. hydrophila, and inhibited its energy metabolism, protein synthesis, protein folding, and DNA replication.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Fisheries
Luke P. Oliver, Timothy J. Bruce, Jie Ma, Evan M. Jones, Kenneth D. Cain
Summary: Burbot have high economic value, low temperature requirements, and fast growth rate, making them a good candidate for cool or cold-water aquaculture. However, limited information exists on the immune systems of this species due to a lack of species-specific tools. In this study, an anti-IgM monoclonal antibody was developed and used to measure the specific antibody response of burbot following exposure to two pathogenic strains. The results demonstrate that the antibody specifically recognized burbot IgM and will be useful in characterizing the immune responses of this fish species.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Weiming Zhong, Puyu Tang, Ting Liu, Tianyu Zhao, Jiajing Guo, Zhipeng Gao
Summary: This study prepared Linalool nano-emulsion (LN) with different medium chain triglycerides (MCT) concentrations using the ultrasonic method. LN showed good stability and strong antibacterial activity against Aeromonas hydrophila, with minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values of 0.3125% and 0.625% v/v, respectively. The nano-emulsion exhibited a disruptive effect on cell membranes, highlighting its potential in controlling and preventing A. hydrophila in aquaculture.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Fisheries
Mustafa Ture, Ayse Cebeci, Ilhan Altinok, Elif Aygur, Nihal Caliskan
Summary: In this study, several lytic phages against A. hydrophila were isolated and characterized. These phages demonstrated cytotoxicity against A. hydrophila strains in vitro and in vivo, but showed no impact on fish or cell lines. Therefore, these phages can be used safely as a cocktail for treating A. hydrophila infection.
Article
Medicine, Research & Experimental
Siqi Xu, Hongsheng Li, Xiaoyan Wu, Jianwei Guo, Jiaoli Zhang, Xuqi Hu
Summary: This study aimed to evaluate the necessity of Western blotting (WB) in samples with inconsistent results in detecting anti-TP antibodies by ELISA and TPPA. The results showed that WB confirmation is necessary when ELISA and TPPA results are inconsistent.
EUROPEAN JOURNAL OF MEDICAL RESEARCH
(2022)
Article
Immunology
Yunchao Cao, Tianshun Kou, Liaotian Peng, Hetron Mweemba Munang'andu, Bo Peng
Summary: Aquatic food is an important source of micronutrients for human beings, and aquaculture plays an increasingly important role in providing this food source. However, bacterial infection poses a severe threat to aquaculture and strategies for managing these infections without antibiotics are lacking. This study investigates the metabolic changes of crucian carp infected with Aeromonas hydrophila and identifies fructose as a crucial biomarker. Supplementation of fructose increases fish survival rate by enhancing the expression of lysozyme and complement 3, leading to reduced bacterial load. These findings demonstrate a metabolism-based approach to manage bacterial infection by modulating the immune response.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Food Science & Technology
Xiaoyi Ma, Cang Wang, Mingcan Qin, Xiaopeng Tian, Shihui Fan, Hangtian Zu, Mingsheng Lyu, Shujun Wang
Summary: A DNAzyme targeting Aeromonas hydrophila was developed through in vitro selection, and a fluorescent biosensor was designed for detecting the bacteria efficiently. The biosensor showed stable performance for more than 6 months and was validated for detecting the pathogen in food samples, demonstrating its potential for use in preventing and detecting foodborne pathogens in the food industry.
Article
Virology
Lingting Pan, Dengfeng Li, Wei Lin, Wencai Liu, Chenxin Qu, Minhua Qian, Ruqian Cai, Qin Zhou, Fei Wang, Yigang Tong
Summary: Ahy-yong1 is a newly discovered phage that can effectively treat multi-antibiotic-resistant pathogen A. hydrophila. It has showed strong biofilm removal capacity and protective effect in fish.
Article
Microbiology
Mariana Lourenco Freire, Felipe Dutra Rego, Karine Ferreira Lopes, Lucelia Antunes Coutinho, Rafaella Fortini Queiroz Grenfell, Daniel Moreira Avelar, Glaucia Cota, Marcelo Antonio Pascoal-Xavier, Edward Oliveira
Summary: Cutaneous leishmaniasis (CL) is a globally spreading public health problem, and Brazil has the greatest number of recorded CL cases. This study developed and validated an immunohistochemistry (IHC) technique for CL diagnosis. The IHC technique showed high sensitivity and specificity, and can be used in combination with other diagnostic approaches for controlling CL.
FRONTIERS IN MICROBIOLOGY
(2022)
Review
Immunology
Mave Harshitha, Ashwath Nayak, Somanath Disha, Uchangi Satyaprasad Akshath, Saurabh Dubey, Hetron Mweemba Munang'andu, Anirban Chakraborty, Indrani Karunasagar, Biswajit Maiti
Summary: The application of nanotechnology in aquaculture for developing efficient vaccines has shown great potential. Nanovaccination, through encapsulating fish pathogen antigens and achieving sustained release, enhances immune responses and protective efficacy of vaccines. However, thorough evaluation of the potential toxicity of nanoparticles is necessary before widespread application.
Article
Medicine, General & Internal
Michael Nweke, Gabriel Ogun, Amos Adeleye, Clement A. Okolo, Adekunle Adesina
Summary: This study aimed to describe the histological features and expression pattern of selected markers in astrocytic neoplasms. The findings showed similarities in epidemiological trends with global instances and suggested that optimal stratification for astrocytomas can be achieved using a combination of IDH-1/EGFR immunohistochemistry.
INTERNATIONAL JOURNAL OF CLINICAL PRACTICE
(2021)
Article
Food Science & Technology
Jing Dong, Tianhui Yan, Qiuhong Yang, Yi Song, Bo Cheng, Shun Zhou, Yongtao Liu, Xiaohui Ai
Summary: Aeromonas hydrophila is a common pathogen in freshwater aquaculture that can also cause health problems in humans. Antibiotic-resistant strains of A. hydrophila are a concern as they limit antibiotic usage and can lead to treatment failure. Additionally, antibiotic residues in aquatic products pose a risk to quality and safety. This study explores alternative strategies to combat infections caused by antibiotic-resistant bacteria, focusing on palmatine, a compound that inhibits the expression of a key virulence factor in A. hydrophila. The findings suggest that palmatine could be a promising treatment for A. hydrophila-associated infections in aquaculture.
Article
Microbiology
Pengji Zhou, Haiyan Huang, Jiaoyang Lu, Zirong Zhu, Junyan Xie, Liqiu Xia, Sisi Luo, Kexuan Zhou, Wenhui Chen, Xuezhi Ding
Summary: The study demonstrated the enhanced antibacterial effect of BaX030 through compound mutagenesis, leading to potential applications in aquaculture. The mutated strain N-11 showed significant increase in the production of macrolactin A and oxydifficidin, as well as elevated expression of immune factors in grass carp. The study identified a probiotic strain with promising antibacterial activity for aquaculture production, offering a new strategy for discovering strains with higher biological activity.
MICROBIOLOGICAL RESEARCH
(2021)
Article
Oncology
Orapan Manajit, Siwaporn Longyant, Paisarn Sithigorngul, Parin Chaivisuthangkura
MOLECULAR MEDICINE REPORTS
(2018)
Article
Fisheries
Pradit Wangman, Siwaporn Longyant, Suparat Taengchaiyaphum, Saengchan Senapin, Paisarn Sithigorngul, Parin Chaivisuthangkura
Article
Fisheries
Tanapon Soonthonsrima, Pradit Wangman, Parin Chaivisuthangkura, Chalinan Pengsuk, Paisarn Sithigorngul, Siwaporn Longyant
AQUACULTURE RESEARCH
(2019)
Article
Fisheries
Akapon Vaniksampanna, Siwaporn Longyant, Walaiporn Charoensapsri, Paisarn Sithigorngul, Parin Chaivisuthangkura
FISH & SHELLFISH IMMUNOLOGY
(2019)
Article
Biophysics
Jantana Kampeera, Patiya Pasakon, Chanpen Karuwan, Narong Arunrut, Assawapong Sappat, Sarawut Sirithammajak, Nipaphorn Dechokiattawan, Thitinun Sumranwanich, Parin Chaivisuthangkura, Puey Ounjai, Surang Chankhamhaengdecha, Anurat Wisitsoraat, Adisorn Tuantranont, Wansika Kiatpathomchai
BIOSENSORS & BIOELECTRONICS
(2019)
Article
Biotechnology & Applied Microbiology
Phongthana Pasookhush, Charles Hindmarch, Paisarn Sithigorngul, Siwaporn Longyant, William G. Bendena, Parin Chaivisuthangkura
Article
Fisheries
Pradit Wangman, Parin Chaivisuthangkura, Suparat Taengchaiyaphum, Chalinan Pengsuk, Paisarn Sithigorngul, Siwaporn Longyant
JOURNAL OF FISH DISEASES
(2020)
Article
Fisheries
Sarocha Jitrakorn, Warachin Gangnonngiw, Malinee Bunnontae, Orapan Manajit, Triwit Rattanarojpong, Parin Chaivisuthangkura, Ha Thanh Dong, Vanvimon Saksmerprome
Article
Fisheries
Phongthana Pasookhush, Akapon Vaniksampanna, Paisarn Sithigorngul, Siwaporn Longyant, Parin Chaivisuthangkura
AQUACULTURE INTERNATIONAL
(2020)
Article
Biotechnology & Applied Microbiology
Chalinan Pengsuk, Pradit Wangman, Parin Chaivisuthangkura, Paisarn Sithigorngul, Siwaporn Longyant
Summary: The study developed a nanogold-based immunochromatographic strip test for rapid and accurate detection of various Vibrio cholerae strains, suitable for preliminary detection of cholera pathogens in fresh seafood. While detection sensitivity can be enhanced through preincubation, the presence of other bacteria in the sample may influence the test results.
JOURNAL OF FOOD SAFETY
(2021)
Article
Fisheries
Terawut Prasitporn, Saengchan Senapin, Akapon Vaniksampanna, Siwaporn Longyant, Parin Chaivisuthangkura
Summary: The study introduced a new method for detecting SDDV virus, utilizing UDG-assisted CPA combined with HNB and LFD technologies, which proved to be rapid, simple, and effective.
JOURNAL OF FISH DISEASES
(2021)
Article
Fisheries
Chutima Srisuk, Chanitcha Choolert, William G. Bendena, Siwaporn Longyant, Paisarn Sithigorngul, Parin Chaivisuthangkura
Summary: This study isolated the cDNA of hemocyanin isoform 2 of the prawn Macrobrachium rosenbergii (MrHc2) and characterized its mRNA expression. The study found that MrHc2 contains three domains and was significantly expressed when challenged with MrNV, suggesting its important role in the innate immune response of M. rosenbergii to MrNV.
JOURNAL OF AQUATIC ANIMAL HEALTH
(2022)
Article
Fisheries
Chanitcha Choolert, Phongthana Pasookhush, Akapon Vaniksampanna, Siwaporn Longyant, Parin Chaivisuthangkura
Summary: In this study, a homolog of TRAF6 called MrTRAF6 was identified and characterized. It was found that MrTRAF6 is involved in various biological processes, including adaptive and innate immunity. Gene expression analysis showed that MrTRAF6 is expressed in all tissues and plays a critical role in antibacterial activity.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Education & Educational Research
Supakan Buatip, Parin Chaivisuthangkura, Pinit Khumwong
INTERNATIONAL JOURNAL OF INSTRUCTION
(2019)
Article
Fisheries
Chutima Srisuk, Saengchan Senapin, William G. Bendena, Siwaporn Longyant, Paisarn Sithigorngul, Parin Chaivisuthangkura
AQUACULTURE RESEARCH
(2018)