Article
Biochemical Research Methods
Ji-Ying Pei, Wen-Feng Yu, Jing-Jing Zhang, Ting-Hao Kuo, Hsin-Hsiang Chung, Jun-Jie Hu, Cheng-Chih Hsu, Ke-Fu Yu
Summary: This study investigates the physiological responses of Pavona decussata coral species under thermal stress using untargeted mass spectrometry-based metabolomics. The findings reveal enhanced lipid hydrolysis and aminolysis, improved energy production efficiency, strengthened immune response, and disrupted holobiont homeostasis as the physiological adaptations of the coral to high-temperature stress.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Review
Chemistry, Analytical
Hugo M. Santos, Luis B. Carvalho, Carlos Lodeiro, Goncalo Martins, Ines L. Gomes, Wilson D. T. Antunes, Vanessa Correia, Maria M. Almeida-Santos, Helena Rebelo-de-Andrade, Antonio P. A. Matos, J. L. Capelo
Summary: This work presents a tutorial on the capabilities of bioanalytical mass spectrometry in detecting and differentiating viruses at the peptide level, as well as providing diagnosis and prognosis for infected patients. It offers insights into the possibilities of current high-resolution mass spectrometry technology and bioinformatics.
MICROCHEMICAL JOURNAL
(2023)
Review
Medical Laboratory Technology
Philippe Massonnet, Elodie Grifnee, Jordi Farre-Segura, Justine Demeuse, Loreen Huyghebaert, Thomas Dubrowski, Patrice Dufour, Matthieu Schoumacher, Stephanie Peeters, Caroline Le Goff, Etienne Cavalier
Summary: Immunocapture is a mature method for sample preparation in quantitation of peptides and proteins. This review provides an overview of clinical applications of immunocapture methods, including protocols with and without enzymatic digestion. The advantages and limitations of these approaches are discussed, along with challenges related to mass spectrometer selection. The potential of these techniques in proteomics-based clinical medicine is demonstrated, paving the way for personalized medicine.
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
(2023)
Review
Medical Laboratory Technology
Stephanie Rankin-Turner, Liam M. Heaney
Summary: Mass spectrometry (MS) has become a powerful and versatile tool in clinical laboratory, with advancements in biomarker detection, protein measurement, therapeutic drug monitoring, and automation leading to potential revolution in clinical testing.
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
(2023)
Article
Biochemical Research Methods
Runmin Yang, Jingjing Ma, Shu Zhang, Yu Zheng, Lusheng Wang, Daming Zhu
Summary: This study introduces a new storage format called mzMD for MS datasets and presents an algorithm to query this format for summarizing a given data window. Experimental results demonstrate the high speed and stability of mzMD in retrieving high-quality data window summaries.
Article
Chemistry, Multidisciplinary
Hao Gu, Guoxin Dai, Siyu Liu, Yanbing Zhai, Qian Xu, Hongqiang Wu, Wei Xu
Summary: Liquid chromatography tandem mass spectrometry (LC-MS/MS) is an important analytical technique in clinical diagnostics. A compact LC-MS instrument was developed, consisting of an assembled liquid chromatograph and a miniature ion trap mass spectrometer. The use of LC improved the mixture analysis capability and detection sensitivity of the miniature mass spectrometer. This compact LC-MS instrument shows potential for clinical diagnosis.
CHINESE CHEMICAL LETTERS
(2023)
Article
Medical Laboratory Technology
Jordi Farre-Segura, Neus Fabregat-Cabello, Chiara Calaprice, Laurent Nyssen, Stephanie Peeters, Caroline Le Goff, Etienne Cavalier
Summary: A rapid and specific LC-MS/MS method for glucagon quantitation has been developed and validated, showing stability and accuracy in comparison to other immunoassay methods. This method could potentially establish a standardized approach for glucagon analysis in routine care settings.
CLINICA CHIMICA ACTA
(2021)
Article
Biochemical Research Methods
Armaghan Shafaei, Veronica Vamathevan, Jessica Pandohee, Nathan G. Lawler, David Broadhurst, Mary C. Boyce
Summary: This study presents a method for determining short-chain fatty acids in serum using high-resolution mass spectrometry and gas chromatography to identify isomeric interferences, which are overcome through a combination of chromatographic separation and judicious choice of MS fragment ion. The LODs and LOQs of SCFAs using this method were in the range 1 to 7 ng mL(-1) and 3 to 19 ng mL(-1), with a recovery ranging from 94 to 114% over the three concentration ranges tested.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Yuan-Chih Chen, Hsin-Yi Wu, Chih-Wei Chang, Pao-Chi Liao
Summary: Conjugation reactions play a critical role in human metabolism, but identifying conjugated metabolites is challenging. In this study, a strategy called PDMS2E-DIA was proposed for comprehensive profiling of the glucuronide-conjugated metabolome. It successfully identified a large number of glucuronide-conjugated metabolites in human urine samples.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Sakshi Gautam, Alireza Banazadeh, Byeong Gwan Cho, Mona Goli, Jieqiang Zhong, Yehia Mechref
Summary: Researchers successfully achieved efficient separation of glycan isomers using mesoporous graphitized carbon (MGC) material in a 1 cm-long column, showing reproducibility and stability in both capillary columns and packed nanospray emitters.
ANALYTICAL CHEMISTRY
(2021)
Article
Plant Sciences
Trevor B. Romsdahl, Jean-Christophe Cocuron, Mackenzie J. Pearson, Ana Paula Alonso, Kent D. Chapman
Summary: This study introduces a stepwise targeted lipidomics approach to characterize polar and non-polar lipid classes. By using hydrophilic interaction chromatography and reverse phase chromatography, changes among different lipid molecular species were found, and potential metabolic explanations were discussed.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Isabel Ten-Domenech, Alvaro Solaz-Garcia, Inmaculada Lara-Canton, Alejandro Pinilla-Gonzalez, Anna Parra-Llorca, Maximo Vento, Guillermo Quintas, Julia Kuligowski
Summary: The GSH-to-GSSG ratio is critical for intracellular redox status. A new LC-MS method for measuring GSH and GSSG in DBS samples was developed, providing a convenient alternative to liquid blood samples. The levels of GSH-NEM and GSSG measured in DBS samples were comparable to those obtained from liquid blood samples in clinical scenarios.
Article
Plant Sciences
Ilaria Chiocchio, Nerea Perez Andres, Redouan Adam Anaia, Nicole M. van Dam, Fredd Vergara
Summary: There are qualitative and quantitative differences in the steroidal glycosides profile between primary and adventitious roots of Solanum dulcamara, which can be used to evaluate the bioactivity of these molecules in belowground ecosystems.
Article
Chemistry, Analytical
Lauren M. Bishop, Tong Shen, Oliver Fiehn
Summary: Accurate and reproducible quantification of endogenous lipids is crucial for large-scale lipidomic analyses. Many lipids in liquid chromatography-electrospray ionization-mass spectrometry form various adducts with buffer components, and the mechanisms and conditions affecting adduct formation are poorly understood. Variations in adduct ratios can lead to significant inaccuracies during quantification, and the use of multiple adduct ions improves accuracy compared to using a single adduct ion.
ANALYTICAL CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Moritz Lasse, Anja R. Stampfli, Thomas Orban, Roshit K. Bothara, Juliet A. Gerrard, Antony J. Fairbanks, Neil R. Pattinson, Renwick C. J. Dobson
Summary: The study identified acrolein modifications on hemoglobin using mass spectrometry and found that these modifications have minimal impact on the secondary structure of hemoglobin. Acrolein modifications promote the formation of crosslinked octamers and alter the response to O2 binding. The study provides qualitative evidence for potential targeted mass spectrometric or immunometric assays for acrolein modified hemoglobin as an oxidative stress marker.
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2021)
Article
Multidisciplinary Sciences
Jean-Baptiste Raina, Bennett S. Lambert, Donovan H. Parks, Christian Rinke, Nachshon Siboni, Anna Bramucci, Martin Ostrowski, Brandon Signal, Adrian Lutz, Himasha Mendis, Francesco Rubino, Vicente I. Fernandez, Roman Stocker, Philip Hugenholtz, Gene W. Tyson, Justin R. Seymour
Summary: This study demonstrates the chemotactic responses of marine bacteria and archaea towards microscale hotspots of phytoplankton-derived dissolved organic matter (DOM). The specific chemical cues attract different prokaryotic populations, which play important roles in biogeochemical processes and ecological interactions in the marine food web.
Article
Microbiology
Madeleine J. H. van Oppen, Jean-Baptiste Raina
ENVIRONMENTAL MICROBIOLOGY
(2023)
Article
Environmental Sciences
Michaela E. Miller, Cherie A. Motti, Mark Hamann, Frederieke J. Kroon
Summary: This study examined the ecological risk of microplastics (MPs) in coral reef ecosystems by comparing MP contamination in a simple food web with that in the surrounding environment. Samples were collected from different trophic levels and environmental matrices, and MPs were found to bioconcentrate but not biomagnify. The complexity of marine food webs and the heterogeneity of MPs in the environment highlight the importance of trophic transfer as a pathway of exposure.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Engineering, Environmental
Amanda L. Dawson, Marina F. M. Santana, Joost L. D. Nelis, Cherie A. Motti
Summary: Significant progress has been made in the harmonisation of methods for microplastic analysis, but the analysis and interpretation of control data has been overlooked. Current consensus on the best method for utilizing control data is lacking, resulting in arbitrary use of methods. This study tested 51 methods and found that only 7 methods showed promise in correcting background contamination, suggesting LOD/LOQ methods or statistical analysis comparing means should be used in future microplastic studies.
JOURNAL OF HAZARDOUS MATERIALS
(2023)
Article
Environmental Sciences
Omar Mendoza-Porras, Thao V. Nguyen, Rohan M. Shah, Peter Thomas -Hall, Lee Bastin, Dione J. Deaker, Cherie A. Motti, Maria Byrne, David J. Beale
Summary: This study investigated the metabolite and lipid profiles of Crown-of-Thorns Starfish (COTS) tissues, including eggs. A total of 410 metabolites and 367 lipids were identified, with amino acids and peptides being the most abundant. COTS eggs had more diverse asterosaponins compared to other tissues. Metabolite and lipid profiles provided insights into tissue-specific processes and potential control strategies of COTS populations. Additionally, COTS was found to be a novel source of molecules with therapeutic and cosmetic properties.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Environmental Sciences
Alexandra M. Gulizia, Kishan Patel, Bronson Philippa, Cherie A. Motti, Lynne van Herwerden, George Vamvounis
Summary: Plastic pollution in the oceans is a growing concern, especially due to the presence of toxic additives. This study aims to understand the leaching properties of plasticisers from microplastics. The leaching behaviors of different plasticisers were investigated under controlled aqueous conditions, and their release rates were modeled using diffusion and boundary layer models. The results provide valuable information for predicting plasticiser concentration and assessing toxicity responses and environmental water quality.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Ecology
Florian U. U. Moeller, Craig W. W. Herbold, Arno Schintlmeister, Maria Mooshammer, Cherie Motti, Bettina Glasl, Katharina Kitzinger, Faris Behnam, Margarete Watzka, Thomas Schweder, Mads Albertsen, Andreas Richter, Nicole S. S. Webster, Michael Wagner
Summary: Marine sponges play a critical role in marine ecosystems as filter-feeders and reef-builders, and they harbor dense communities of microbes. These microbes contribute to the processing of dissolved organic matter (DOM). A study using metaproteogenomics and laboratory incubations found that a dominant gammaproteobacterial symbiont in a marine sponge expressed a pathway for the import and dissimilation of taurine, a common metabolite in marine sponges. The symbiont also imported and used DMSP as a carbon and sulfur source. These findings highlight the importance of biogenic sulfur compounds in the interaction between marine sponges and their microbial symbionts.
Article
Plant Sciences
Caleb C. Butler, Kira E. Turnham, Allison M. Lewis, Matthew R. Nitschke, Mark E. Warner, Dustin W. Kemp, Ove Hoegh-Guldberg, William K. Fitt, Madeleine J. H. van Oppen, Todd C. LaJeunesse
Summary: This study identified five new species in the genus Cladocopium, which are widespread and have diverse hosts. These findings are important for understanding ecological research, genetic research, and conservation efforts in the face of environmental changes.
JOURNAL OF PHYCOLOGY
(2023)
Article
Multidisciplinary Sciences
Meaghan K. Smith, Bronwyn A. Rotgans, Tomas Lang, Ryan Johnston, Tianfang Wang, Saowaros Suwansa-ard, Utpal Bose, Nori Satoh, Michaela Egertova, Michael R. Hall, Maria Bryne, Maurice R. Elphick, Cherie A. Motti, Scott F. Cummins
Summary: This study investigates the ultrastructure of the radial nerve cords (RNCs) in the Pacific crown-of-thorns starfish and identifies various secreted proteins and neuropeptides, providing insight into the ecological impact, movement, and regeneration of this coral predator.
SCIENTIFIC REPORTS
(2023)
Correction
Multidisciplinary Sciences
Meaghan K. K. Smith, Bronwyn A. A. Rotgans, Tomas Lang, Ryan Johnston, Tianfang Wang, Saowaros Suwansa-ard, Utpal Bose, Nori Satoh, Michaela Egertova, Michael R. R. Hall, Maria Byrne, Maurice R. R. Elphick, Cherie A. A. Motti, Scott F. F. Cummins
SCIENTIFIC REPORTS
(2023)
Article
Biology
Wing Yan Chan, David Rudd, Madeleine J. H. van Oppen
Summary: Microbial symbionts in multicellular hosts often localize within specific body structures or cell types, playing critical roles in host health and nutrient exchange. However, conventional methods for measuring host-microbe metabolite exchange have limitations. In this study, we developed a mass spectrometry imaging workflow that allows for in situ analysis of host and symbiont metabolomes in a cnidarian animal without the need for isotopic labeling or skeleton decalcification. Our findings reveal the importance of specific lipids in regulating symbiont acquisition and distribution, and demonstrate the impact of symbiont identity on host metabolism.
LIFE SCIENCE ALLIANCE
(2023)
Article
Evolutionary Biology
Hugo J. Scharfenstein, Carlos Alvarez-Roa, Lesa M. Peplow, Patrick Buerger, Wing Yan Chan, Madeleine J. H. van Oppen
Summary: Through chemical mutagenesis and thermal selection, Durusdinium trenchii, Fugacium kawagutii, and Symbiodinium pilosum strains of Symbiodiniaceae showed increased photosynthetic efficiency under thermal stress. However, there were trade-offs in the growth rates of the heat-evolved D. trenchii lineage at both ambient and elevated temperatures. Reduced nutrient uptake rates in F. kawagutii and S. pilosum heat-evolved lineages suggest changes in nutrition resource usage and allocation processes. These findings enhance our understanding of the physiological responses and adaptive capacity of Symbiodiniaceae cultures to thermal selection.
EVOLUTIONARY APPLICATIONS
(2023)
Article
Biodiversity Conservation
Wing Yan Chan, Luka Meyers, David Rudd, Sanjida H. Topa, Madeleine J. H. van Oppen
Summary: Experimental evolution of coral microalgal symbionts can enhance the thermotolerance of adult corals without trade-off against growth under ambient temperature. This study demonstrates that heat-evolved symbionts can persist in corals for a long time and potentially provide long-term benefits to coral thermotolerance.
GLOBAL CHANGE BIOLOGY
(2023)
Article
Ecology
Cathie A. Page, Christine Giuliano, Kelley Meehan, Rebecca Fisher, Cherie A. Motti, Andrew P. Negri, Carly J. Randall
Summary: High macroalgae cover can reduce coral larval settlement and hinder coral recovery. In this study, the allelopathic effect of Lobophora sp. on the settlement of 9 coral species was tested. The results showed that Lobophora extracts had varied effects on different coral species, indicating interspecific variation in Lobophora sensitivity.
MARINE ECOLOGY PROGRESS SERIES
(2023)
Article
Ecology
Keren Maor-Landaw, Marion Eisenhut, Giada Tortorelli, Allison van de Meene, Samantha Kurz, Gabriela Segal, Madeleine J. H. van Oppen, Andreas P. M. Weber, Geoffrey I. McFadden
Summary: The symbiotic partnership between corals and dinoflagellate algae is crucial for coral reefs. The transporter protein BmSWEET1 in dinoflagellate cells is responsible for transferring glucose from algae to the host coral tissue, providing the necessary energy for coral reefs. This study provides insights into the mechanism of nutrient exchange in coral-algae symbiosis.
ISME COMMUNICATIONS
(2023)
Article
Ecology
Katja J. Geiger, Julio Arrontes, Antonella Rivera, Consolacion Fernandez, Jorge Alvarez, Jose Luis Acuna
Summary: A two-year experiment was conducted to investigate the impact of Pollicipes pollicipes harvest on intertidal community structure and ecological diversity. The study found that intensive exploitation resulted in a decrease in P. pollicipes and Mytilus spp. coverage, while Chthamalus spp. and Corallina spp. increased. The recovery of P. pollicipes aggregations was slow and variable, but their coverage increased under non-extracted conditions.
JOURNAL OF EXPERIMENTAL MARINE BIOLOGY AND ECOLOGY
(2024)
Article
Ecology
Daniel Castro Martignago, Leandro Godoy, Amanda Pereira Amaral, Guendalina Turcato Oliveira
Summary: This study evaluates the effects of bleaching on the oocytes of the Mussismilia harttii coral and investigates the strategies employed by these cells to maintain antioxidant balance and cellular homeostasis. The research finds that bleached coral oocytes experience lipid damage, but are still able to maintain their quality and potentially elongate their lifespan and fertilization capability. This response may be linked to an intensification of heterotrophy in bleached corals.
JOURNAL OF EXPERIMENTAL MARINE BIOLOGY AND ECOLOGY
(2024)
