Article
Biochemistry & Molecular Biology
Xiaopeng Lu, Min Xu, Qian Zhu, Jun Zhang, Ge Liu, Yantao Bao, Luo Gu, Yuan Tian, He Wen, Wei-Guo Zhu
Summary: Histone methyltransferase KMT5A interacts with E3 ligases RNF8 and RNF168 to establish histone modification status for DNA damage repair. KMT5A increases H4K20 monomethylation at DSBs and enhances RNF168's activity in catalyzing H2A ubiquitination, linking the two processes. The interaction between H2A acidic patch and KMT5A residues R188/R189 is crucial for KMT5A-mediated regulation of H2A ubiquitination.
Review
Cell Biology
Antonio Rivas-Dominguez, Nuria Pastor, Laura Martinez-Lopez, Julia Colon-Perez, Beatriz Bermudez, Manuel Luis Orta
Summary: The high incidence of colorectal cancer in developed countries suggests a significant role of the environment as a causative factor. Dysbiosis, characterized by an unbalanced microbiota, can lead to intestinal damage and inflammation, promoting the occurrence of CRC.
Article
Microbiology
Linda C. Horianopoulos, Christopher W. J. Lee, Kerstin Schmitt, Oliver Valerius, Guanggan Hu, Melissa Caza, Gerhard H. Braus, James W. Kronstad
Summary: This study identifies a nuclear J domain protein, Dnj4, in the fungal pathogen Cryptococcus neoformans, demonstrating its role as a histone chaperone important for maintaining genome integrity and responding to DNA damage. Dnj4 regulates iron homeostasis and plays a crucial role in DNA damage response in C. neoformans. Additionally, Dnj4 functions as a conserved histone chaperone disrupting endogenous histone chaperoning machinery, suggesting its potential in understanding biological processes across different organisms.
Review
Genetics & Heredity
Yongxin Li, Yan Tong, Jiaqi Liu, Jianlin Lou
Summary: DNA is crucial for organism development and function. MiRNA, a type of non-coding RNA, may play an important role in the DNA damage response by influencing processes like cell cycle, DNA repair, and apoptosis, thereby impacting genomic stability and tumorigenesis.
FRONTIERS IN GENETICS
(2022)
Article
Cell Biology
Marian Kupculak, Fengxiang Bai, Qiang Luo, Yasunaga Yoshikawa, David Lopez-Martinez, Hannan Xu, Stephan Uphoff, Martin A. Cohn
Summary: This study identifies 10 phosphorylation sites on FANCD2, which are phosphorylated by ATR in response to DNA interstrand crosslinks (ICLs). These phosphorylation events are critical for loading of the FANCD2/FANCI complex onto chromosomes and subsequent monoubiquitination. The regulation of these phosphorylation events is important for maintaining the proper function of FANCD2 in cells.
Article
Cell Biology
Benyu Su, David Lim, Chenyang Qi, Zhongwei Zhang, Junxiao Wang, Fengmei Zhang, Chao Dong, Zhihui Feng
Summary: Cell cycle checkpoint kinases play a crucial role in protecting against replicative stress. Valproic acid (VPA), a histone deacetylase inhibitor, was found to promote breast cancer cell progression into G2/M phase while protecting normal cells by regulating PPP2R2A and Chk1 phosphorylation. The expression levels of PPP2R2A and pChk1 were also associated with patient survival.
CELL DEATH & DISEASE
(2023)
Article
Multidisciplinary Sciences
Larissa Milano, Clara F. Charlier, Rafaela Andreguetti, Thomas Cox, Eleanor Healing, Marcos P. Thome, Ruan M. Elliott, Leona D. Samson, Jean-Yves Masson, Guido Lenz, Joao Antonio P. Henriques, Axel Nohturfft, Lisiane B. Meira
Summary: The study investigates the effects of the alkylating agent MMS on gene expression in mouse liver using mice deficient in the enzyme Aag. It shows that Aag deficiency leads to a reduction in the cellular stress response induced by alkylation.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Biochemistry & Molecular Biology
Xinlin Xu, Yixi Xu, Ruiyuan Guo, Ran Xu, Congcong Fu, Mengtan Xing, Hiroyuki Sasanuma, Qing Li, Minoru Takata, Shunichi Takeda, Rong Guo, Dongyi Xu
Summary: Cell-based, in vitro and in vivo assays demonstrate that Fanconi anemia factors participate in a BRCA1-dependent BIR-like pathway, aiding in the restarting of stalled replication forks, while persistent replication stress contributes to FA pathogenesis. This suggests that the FA pathway is one of the BIR-like pathways and replication stress plays a crucial role in FA disease progression.
NATURE STRUCTURAL & MOLECULAR BIOLOGY
(2021)
Article
Pharmacology & Pharmacy
Yu-Fei Chen, Yan-Chun Pang, Han-Chen Wang, Pei-En Wu, Zi-Jie Chen, Da Huang, Dong-Ling Peng, Yong-Ming Yan, Changhui Liu, Li-Chuan Wu, Xiang-Zhen Fan, Yong-Xian Cheng, Yong-Qiang Liu
Summary: The bioactive compound ArC in Centipeda minima extracts has synergistic cytotoxic effects with DNA cross-linking drugs in non-small cell lung cancer cells, by inhibiting the mTOR/E2F1/FANCD2 signaling axis and suppressing DNA damage response.
BRITISH JOURNAL OF PHARMACOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Mi Ae Kang, Jong-Soo Lee
Summary: CTCF, a highly conserved multifunctional DNA-binding protein with 11 zinc fingers, plays crucial roles in diverse genomic processes such as transcriptional regulation, insulation, genome imprinting, and maintenance of genome organization. Recent findings reveal that CTCF is involved in DNA double-strand break (DSB) repair through homologous recombination (HR), facilitating accurate restoration of broken DNA sequences. Understanding the functional crosstalks between CTCF and other HR factors may shed light on the molecular basis of various human diseases and the diverse functions of CTCF in genome biology.
Article
Multidisciplinary Sciences
Raji R. Nair, Joshua Hsu, Justin T. Jacob, Christopher M. Pineda, Ryan P. Hobbs, Pierre A. Coulombe
Summary: The high levels of the intermediate filament protein keratin 17 (K17) are associated with poor prognoses for several human carcinomas. Lack of K17 delays onset of tumorigenesis in mouse models and K17 impacts chromatin architecture, gene expression, and cell proliferation in the nucleus of tumor keratinocytes. The presence of nuclear-localized K17 is required for early stages of DNA damage and repair cascade, affecting cell survival and leading to poor clinical outcomes in human carcinomas.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Review
Biochemistry & Molecular Biology
Chibin Cheng, Daniel Seen, Chunwen Zheng, Ruijie Zeng, Enmin Li
Summary: RhoA plays a crucial role in DNA damage response by interacting with key components of DNA repair and cell cycle control. Understanding the role of RhoA in DDR can provide insights into DDR mechanisms and aid in the development of cancer therapies in the future.
Review
Genetics & Heredity
Annabelle Shaw, Monika Gullerova
Summary: Non-coding RNA plays a crucial role in the DNA damage response, influencing DNA repair through mechanisms such as recruiting repair factors and regulating expression. DDR can be communicated intercellularly via exosomes, resulting in bystander responses in healthy cells and potentially impacting radioresistance and cancer treatment.
Article
Environmental Sciences
Yan Jin, Yiting Li, Shiyi He, Yijun Ge, Yun Zhao, Ke Zhu, Andong He, Siyu Li, Siyu Yan, Chao Cao
Summary: PM2.5 induces airway inflammation and DNA damage, and the ATM gene plays a role in promoting this inflammation.
ENVIRONMENTAL TOXICOLOGY
(2023)
Article
Environmental Sciences
Min Zhang, Hailin Xia, Min Yu, Li Ju, Yun Xiao, Lijin Zhu
Summary: The study revealed that chrysotile caused dose-dependent DNA damage in MeT-5A and BEAS-2B cells, with MeT-5A cells showing more persistent damage. PARP1-deficient cells were found to be more sensitive to chrysotile-induced DNA damage compared to normal cells. Furthermore, the repair experiments showed that all cell lines were able to recover from the damage over time, indicating the important role of PARP1 in early repair of DNA damage in cells exposed to chrysotile.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2021)