Article
Biotechnology & Applied Microbiology
Xubing Long, Ziwei Yang, Yang Li, Qinqin Sun, Xiaojuan Li, Ersheng Kuang
Summary: The study reveals that BRLF1 regulates cellular and viral gene expression and transcription during EBV primary infection, impacting pathways such as DNA replication and metabolism. BRLF1 binds to transcription factors and spliceosome components, influencing RNA polymerase II-dependent transcription and pre-mRNA splicing.
Review
Biochemistry & Molecular Biology
Jessica J. Roetman, Minna K. Apostolova, Mary Philip
Summary: Thirteen percent of cancers worldwide are associated with viral infections, but few infected individuals develop cancer. Viral infections trigger immune signaling pathways and activate tumor suppressors, indicating that tumor suppressors may have evolved primarily to combat viral infections.
Article
Virology
Mingqian Xu, Liang Zhang, Jinfu Feng, Shuaibing Yang, Yixuan Wang, Yuyi Wang, Meiyang Chen, Li Zhou, Junjie Zhang, Qingsong Qin
Summary: EBV-associated gastric cancer is a subtype of gastric cancer characterized by the presence of EBV genome and limited viral gene expression in malignant epithelial cells. Two EBV-positive gastric cancer cell lines have been established to study the role of EBV in different contexts of gastric carcinogenesis. These cell lines can serve as models to investigate the role of EBV in gastric cancer and identify new therapeutics against EBVaGC.
JOURNAL OF MEDICAL VIROLOGY
(2023)
Article
Multidisciplinary Sciences
Deborah K. Johnson, Kaylia M. Reynolds, Brian D. Poole, Matthew D. Montierth, Vera M. Todd, April Barnado, Mary F. Davis
Summary: Patients with autoimmune disorders have altered cancer risks compared to the general population, with a strong association between viral infection and increased cancer risk. SLE and MS patients are more susceptible to viral infections, and preventing viral infections through vaccination may help control cancer risk for these patients.
Article
Microbiology
Katherine A. Willard, Ashley P. Barry, Cliff I. Oduor, John Michael Ong'echa, Jeffrey A. Bailey, Ann M. Moormann, Micah A. Luftig
Summary: In this study, we describe an unusual spontaneous lytic phenotype in EBV strains isolated from Kenyan endemic Burkitt lymphoma patients. These findings provide insight into viral and host factors involved in this process and the potential contribution of promiscuous lytic activity to the formation of EBV-associated diseases.
Article
Immunology
Mariann Gyoengyoesi, Dominika Lukovic, Julia Mester-Tonczar, Katrin Zlabinger, Patrick Einzinger, Andreas Spannbauer, Victor Schweiger, Katharina Schefberger, Eslam Samaha, Jutta Bergler-Klein, Martin Riesenhuber, Christian Nitsche, Christian Hengstenberg, Patrick Mucher, Helmuth Haslacher, Monika Breuer, Robert Strassl, Elisabeth Puchhammer-Stoeckl, Christian Loewe, Dietrich Beitzke, Ena Hasimbegovic, Thomas A. Zelniker
Summary: This study evaluated the reactivation of parvovirus-B19 and Herpesviridae family members in patients with long-COVID syndrome and found that vaccination with monovalent COVID-19 vaccines was associated with reduced fatigue and multiorgan symptoms. It also led to lower levels of DNA virus-related IgM, plasma IgG subfractions, cytomegalovirus IgG and IgM, and EBV IgM titers.
Article
Microbiology
Rachel Hood Edwards, Nancy Raab-Traub
Summary: This study evaluated the impact of EBV and BART lncRNAs on cellular expression, showing that they induce changes in cellular miRs and lncRNAs. In vivo expression changes reflect increased cellular miRs in all tumors, while the BART lncRNA may inhibit the function of induced cellular miRs. The study provides insights into the total effects of EBV and BART lncRNA on cellular RNA expression.
Article
Multidisciplinary Sciences
Emmanuela N. Bonglack, Joshua E. Messinger, Jana M. Cable, James Ch'ng, K. Mark Parnell, Nicolas M. Reinoso-Vizcaino, Ashley P. Barry, Veronica S. Russell, Sandeep S. Dave, Heather R. Christofk, Micah A. Luftig
Summary: The study found that EBV infection directly induced temporal expression of MCT1 and MCT4 in B lymphocytes, with MCT1 being crucial for early B cell proliferation and MCT4 upregulation leading to acquired resistance to MCT1 antagonism in LCLs. Dual inhibition of MCT1/4 resulted in growth arrest and lactate buildup in LCLs, suggesting a potential therapeutic approach for targeting viral lymphomas associated with EBV and KSHV.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Microbiology
Quincy Rosemarie, Elijah Kirschstein, Bill Sugden
Summary: We have discovered how Epstein-Barr virus (EBV) induces the reorganization of cellular chromatin, with viral DNA replication occurring in the chromatin-free regions. Five families of DNA viruses induce similar reorganization, and they infect a variety of hosts. The viral genes and processes required for EBV's chromatin reorganization have been identified, and a two-step model for the process has been proposed. The importance of chromatin reorganization in viral life cycles is emphasized. Overall, a 9 out of 10 score is given for the importance of this research.
Article
Chemistry, Analytical
Yunyun Hu, Ye Tian, Haonan Di, Chengfeng Xue, Yanping Zheng, Bin Hu, Qin Lin, Xiaomei Yan
Summary: This study utilized nanoflow cytometry to examine surface proteins on extracellular vesicles and found that EVSUM5 and EVSUM2 can effectively differentiate NPC patients from NPG patients, providing a more accurate diagnostic method.
ANALYTICAL CHEMISTRY
(2022)
Review
Virology
Guo-Long Bu, Chu Xie, Yin-Feng Kang, Mu-Sheng Zeng, Cong Sun
Summary: This review article discusses the tropism and molecular mechanism of EBV infection. The interactions between the virus and B cells and epithelial cells differ, and the entry process relies on heterogenous glycoprotein-receptor interactions.
Review
Biochemistry & Molecular Biology
Leila Sawada, Antonio Carlos Rosario Vallinoto, Igor Brasil-Costa
Summary: EBV is an oncovirus associated with various types of cancer, with the reason for cancer development in only some infected individuals still unknown. EBV-associated cancers are more aggressive and resistant to treatment compared to EBV-negative cancers, making monoclonal antibodies targeting immune checkpoints a potential therapy.
Article
Gastroenterology & Hepatology
Miki Miura, Hirotaka Shimizu, Daisuke Saito, Jun Miyoshi, Minoru Matsuura, Takahiro Kudo, Daisuke Hirayama, Masashi Yoshida, Katsuhiro Arai, Itaru Iwama, Hiroshi Nakase, Toshiaki Shimizu, Tadakazu Hisamatsu
Summary: This study was the first multicenter survey of EBV infection in Japanese patients with IBD, revealing that approximately 30% of the patients were EBV-uninfected. Age was found to be a significant factor for anti-VCA IgG seropositivity.
JOURNAL OF GASTROENTEROLOGY
(2021)
Article
Biochemistry & Molecular Biology
Andrii A. Zaremba, Polina Y. Zaremba, Maxim O. Platonov
Summary: Epstein-Barr virus is a DNA-containing virus associated with approximately 1% of all cancers. This study focuses on a low molecular weight inhibitor called EBAI, which shows high affinity for the BHRF1 protein of the virus. In vitro studies are needed to fully evaluate EBAI's potential as an inhibitor of the virus's antiapoptotic protein.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2023)
Article
Oncology
Atsushi Kondo, Aya Shinozaki-Ushiku, Hirofumi Rokutan, Akiko Kunita, Masako Ikemura, Hiroharu Yamashita, Yasuyuki Seto, Genta Nagae, Kenji Tatsuno, Hiroyuki Aburatani, Daizo Koinuma, Tetsuo Ushiku
Summary: In this study, we report eight cases of EBV-associated gastric carcinoma (EBVaGC) with heterogenous EBER expression. Through detailed histological assessment and genetic/epigenetic analysis, we identified collision tumors of different origins in two patients and shared alterations between EBER-positive and -negative components in the other six patients. This study provides important insights into the histological features and genetic/epigenetic alterations in EBVaGC and the clinical significance of losing EBV in tumor progression and immune evasion.
JOURNAL OF PATHOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Jiayu Wang, Noemi Nagy, Maria G. Masucci
Article
Oncology
Sylvain Peuget, Jiawei Zhu, Gema Sanz, Madhurendra Singh, Massimiliano Gaetani, Xinsong Chen, Yao Shi, Amir Ata Saei, Torkild Visnes, Mikael S. Lindstrom, Ali Rihani, Lidia Moyano-Galceran, Joseph W. Carlson, Elisabet Hjerpe, Ulrika Joneborg, Kaisa Lehti, Johan Hartman, Thomas Helleday, Roman Zubarev, Galina Selivanova
Article
Medicine, Research & Experimental
Shi Yong Neo, Ying Yang, Julien Record, Ran Ma, Xinsong Chen, Ziqing Chen, Nicholas P. Tobin, Emily Blake, Christina Seitz, Ron Thomas, Arnika Kathleen Wagner, John Andersson, Jana de Boniface, Jonas Bergh, Shannon Murray, Evren Alici, Richard Childs, Martin Johansson, Lisa S. Westerberg, Felix Haglund, Johan Hartman, Andreas Lundqvist
JOURNAL OF CLINICAL INVESTIGATION
(2020)
Article
Cell Biology
Paivi Yla-Anttila, Soham Gupta, Maria G. Masucci
Summary: Macroautophagy plays a critical role in controlling viral infections, with viruses evolving strategies to interfere with this process. The Epstein-Barr virus-encoded BPLF1 protein regulates selective autophagy, promoting infection and viral production by targeting the cellular defense mechanism.
Article
Biochemistry & Molecular Biology
Linnea Hases, Ahmed Ibrahim, Xinsong Chen, Yanghong Liu, Johan Hartman, Cecilia Williams
Summary: The study reveals sex differences in the transcriptome of normal colon and CRC tissue, with some biomarkers showing sex-specific prognostic value. 20 sex-specific CRC prognostic biomarkers, including ESM1, GUCA2A, and VWA2 for males and CLDN1 and FUT1 for females, are proposed, which can help improve CRC survival rates.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Paivi Yla-Anttila, Maria G. Masucci
Summary: Autophagy is a crucial protein degradation and recycling mechanism that plays a significant role in cellular homeostasis and defense against microbial invasion. It can be non-selective or selective, with different autophagy receptors recognizing specific targets for degradation. Some viruses, like EBV, KSHV, and HCMV, interfere with selective autophagy by regulating the activity of autophagy receptors such as SQSTM1/p62.
BIOCHEMICAL JOURNAL
(2021)
Article
Microbiology
Jinlin Li, Noemi Nagy, Jiangnan Liu, Soham Gupta, Teresa Frisan, Thomas Hennig, Donald P. Cameron, Laura Baranello, Maria G. Masucci
Summary: The study reveals that EBV's BPLF1 can alter the structure of TOP2 in cells, preventing DNA damage response and promoting cell survival and virus replication. This protective effect relies heavily on the expression of TDP2, which aids in DNA repair.
Article
Oncology
Apple Hui Min Tay, Ruben Prieto-Diaz, Shiyong Neo, Le Tong, Xinsong Chen, Valentina Carannante, Bjorn Onfelt, Johan Hartman, Felix Haglund, Maria Majellaro, Jhonny Azuaje, Xerardo Garcia-Mera, Jose M. Brea, Maria Loza, Willem Jespers, Hugo Gutierrez-de-Teran, Eddy Sotelo, Andreas Lundqvist
Summary: This study synthesized five potent A(2B)AR antagonists and a dual A(2A)AR/A(2B)AR antagonist, and evaluated their therapeutic potential in cancer immunotherapy. The results showed that A(2B)AR antagonists can enhance T and NK cell proliferation, increase cytokine production, and boost lymphocyte infiltration into tumor spheroids without altering adhesion molecule expression.
JOURNAL FOR IMMUNOTHERAPY OF CANCER
(2022)
Meeting Abstract
Oncology
X. Chen, E. G. Sifakis, S. Robertson, S. Y. Neo, S-H. Jun, L. Tong, J. Lovrot, A. Tay, R. Hellgren, S. Margolin, J. Bergh, T. Foukakis, J. Lagergren, A. Lundqvist, R. Ma, J. Hartman
ANNALS OF ONCOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Enric Llorens-Bobadilla, Margherita Zamboni, Maja Marklund, Nayanika Bhalla, Xinsong Chen, Johan Hartman, Jonas Frisen, Patrik L. Stahl
Summary: Spatial ATAC is a method that combines transposase-accessible chromatin profiling with barcoded solid-phase capture to study spatially resolved epigenomics. It has been used to uncover the regulatory programs underlying spatial gene expression during mouse organogenesis, lineage differentiation, and human pathology.
NATURE BIOTECHNOLOGY
(2023)
Article
Multidisciplinary Sciences
Xinsong Chen, Emmanouil G. Sifakis, Stephanie Robertson, Shi Yong Neo, Seong-Hwan Jun, Le Tong, Apple Tay Hui Min, John Lovrot, Roxanna Hellgren, Sara Margolin, Jonas Bergh, Theodoros Foukakis, Jens Lagergren, Andreas Lundqvist, Ran Ma, Johan Hartman
Summary: Breast cancer is a complex disease with multiple subtypes, and personalized therapy is needed due to patient-to-patient variability in drug response. This study establishes a whole-tumor cell culture (WTC) ex vivo model from breast tumors, which can accurately predict drug response and has potential for individualized therapy. The WTC model can also help stratify patients for clinical trials and validate biomarkers during drug development.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Multidisciplinary Sciences
Seong-Hwan Jun, Hosein Toosi, Jeff Mold, Camilla Engblom, Xinsong Chen, Ciara O'Flanagan, Michael Hagemann-Jensen, Rickard Sandberg, Samuel Aparicio, Johan Hartman, Andrew Roth, Jens Lagergren
Summary: Functional characterization of cancer clones is important for understanding cancer's evolutionary mechanisms. The authors introduce PhylEx, a method that combines bulk genomics and single-cell RNA sequencing data to reconstruct clonal trees and identify clones. PhylEx outperforms other methods and allows for accurate inference of clonal trees and analysis of cancer.
NATURE COMMUNICATIONS
(2023)
Article
Biotechnology & Applied Microbiology
Caterina Collodet, Kelly Blust, Savvini Gkouma, Emmy Stahl, Xinsong Chen, Johan Hartman, My Hedhammar
Summary: Traditional 2D and 3D cancer models fail to replicate cell-extracellular matrix interactions. Hydrogel-based alternatives lack mechanical support and reproducibility. This study presents a novel strategy using recombinant spider silk to create 3D breast cancer models with a well-defined network environment. The models maintain characteristic expression of markers while demonstrating distinct morphology. Transcriptomic analysis reveals the impact of culture in the silk networks on cell adhesion and migration processes, as well as physiological events related to malignancy. Integration in the silk networks enhances the viability of cells obtained from patients' breast tumors.
BIOENGINEERING & TRANSLATIONAL MEDICINE
(2023)
Meeting Abstract
Oncology
Ioannis Zerdes, Dimitrios Salgkamis, Alexios Matikas, Lars Selander, Kang Wang, Evangelos Tzoras, Emmanouil Sifakis, Susanne Agartz, Xinsong Chen, Mats Hellstrom, Johan Hartman, Jonas Bergh, Theodoros Foukakis, Thomas Hatschek
Article
Oncology
Madhurendra Singh, Xiaolei Zhou, Xinsong Chen, Gema Sanz Santos, Sylvain Peuget, Qing Cheng, Ali Rihani, Elias S. J. Arner, Johan Hartman, Galina Selivanova
BREAST CANCER RESEARCH
(2020)