Article
Veterinary Sciences
Jordi Ribas-Maynou, Marc Llavanera, Yentel Mateo-Otero, Estela Garcia-Bonavila, Ariadna Delgado-Bermudez, Marc Yeste
Summary: This study analyzed eight techniques assessing chromatin damage in pig sperm and found that the extent of chromatin damage is significantly correlated with the percentage of sperm with fragmented DNA in direct methods, but not in indirect ones. The results suggest that there are differences in interpreting sperm chromatin damage between indirect and direct techniques.
FRONTIERS IN VETERINARY SCIENCE
(2021)
Article
Agriculture, Dairy & Animal Science
John Watts
Summary: The study aimed to compare measurements of spermatozoal membrane status in dogs using CASQ and CFDA/PI staining methods. Results showed that measuring MIS percentage with red and red-green filter CASQ appeared to be the most reliable automated methods.
REPRODUCTION IN DOMESTIC ANIMALS
(2021)
Article
Reproductive Biology
Jordi Ribas-Maynou, Estela Garcia-Bonavila, Sergi Bonet, Jaime Catalan, Albert Salas-Huetos, Marc Yeste
Summary: The relationship between sperm DNA fragmentation and reproductive outcomes is inconsistent, and concerns have been raised about its value as a sperm quality parameter. This study found that increasing chromatin decondensation makes the TUNEL assay more sensitive to detect DNA damage in pig sperm.
Article
Chemistry, Inorganic & Nuclear
Manikantha Dunna, Saraswathi Kailasa, Lavudi Saida, P. Jeevan Kumar, Tumma Gurukiran Babu, Kalyani Chepuri, K. Venkateswara Rao
Summary: Nanomedicine shows promise for targeted cancer therapy with improved efficacy and reduced side effects. CeO2 nanostructures have gained attention due to their unique properties for cancer treatment. This study examined the synthesis methods of biocompatible cerium oxide nanoparticles and their cytotoxic activity against cancer cell lines. Results showed that CeO2-P nanoparticles exhibited better performance in inducing apoptosis and had lower toxicity towards lymphocytes compared to CeO2-C nanoparticles. These findings suggest the potential use of CeO2 nanostructures as a promising therapeutic agent in cancer treatment.
INORGANIC CHEMISTRY COMMUNICATIONS
(2023)
Article
Microbiology
Yabin Zhang, Weihua Fan, Chunhong Shao, Jiajia Wang, Yan Jin, Jing Shao, Ying Zhang, Yong Wang
Summary: The study demonstrates that the ultra-rapid antibiotic resistance detection method using SYBR Green I and propidium iodide shows high accuracy and reliability in Klebsiella pneumoniae. This rapid AST can help enable targeted therapy, reduce empirical use of antibiotics, and ultimately alleviate the health and economic burdens of antibiotic resistance.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Multidisciplinary Sciences
Aditya Sarnaik, Apurv Mhatre, Muhammad Faisal, Dylan Smith, Ryan Davis, Arul M. Varman
Summary: The study found that ultra-low temperature storage affects cell viability and protein extraction efficiency, with longer storage leading to lower cell viability but higher protein extraction efficiency.
Article
Andrology
Akiyoshi Osaka, Hiroshi Okada, Sae Onozuka, Takashi Tanaka, Toshiyuki Iwahata, Yukihito Shimomura, Yoshitomo Kobori, Kazutaka Saito, Kouhei Sugimoto
Summary: Sperm DNA fragmentation analysis can be used to assess male fertility. However, there have been no large-scale studies on the sperm DNA fragmentation index (DFI) of Japanese men. This study demonstrates the feasibility of using flow cytometry-based sperm DFI analysis to evaluate male fertility in Japan. Sperm DNA fragmentation analysis can assess sperm functions that cannot be evaluated by ordinary semen analysis.
ASIAN JOURNAL OF ANDROLOGY
(2022)
Article
Environmental Sciences
Kamila Jessie Sammarro Silva, Lyda Patricia Sabogal-Paz
Summary: Giardia and Cryptosporidium are resistant pathogenic protozoa that are important for environmental quality monitoring and sanitation. Viability assessment can be conducted by evaluating membrane integrity and enzyme activity, using different staining methods. Simultaneous staining with a commercial live/dead kit impairs (oo)cyst detection by immunofluorescence assay (IFA) and has poor labeling efficiency. Live G. muris cysts and C. parvum oocysts do not exhibit esterase metabolism fluorescence, possibly due to low enzyme activity and/or thick parasite walls. Further analysis is needed to consider different incubation conditions.
ENVIRONMENTAL TECHNOLOGY
(2022)
Article
Multidisciplinary Sciences
Elham Shojaei, Sona Zare, Afshan Shirkavand, Esmaeil Eslami, Sara Fathollah, Parvin Mansouri
Summary: This study investigates the effects of non-thermal atmospheric pressure plasma on human adipose tissue-derived stem cells and explores the feasibility of using optical spectroscopy as a non-destructive method for cell analysis. The results show that exposed stem cells maintain their stemness and short exposure to plasma is relatively non-toxic. In addition, the plasma can accelerate the cell cycle and promote proliferation, and reflectance spectroscopy proves to be an efficient method for evaluating treatment response.
SCIENTIFIC REPORTS
(2022)
Article
Biochemical Research Methods
Jeremy J. John, Christopher J. May, John G. Bruno
Summary: This method simplifies the rapid detection of live Legionella pneumophila in mixed bacterial populations by using a combined immunofluorescence and fluorescence viability stain technique. By using a cocktail staining solution, it efficiently differentiates between live and dead cells, providing quick analysis results under a microscope.
JOURNAL OF FLUORESCENCE
(2021)
Article
Agriculture, Dairy & Animal Science
Kathan Raval, Nilendu Paul, Pradeep Nag, K. Elango, Yash Pal, R. A. Legha, T. R. Talluri, Arumugam Kumaresan
Summary: The integrity of sperm acrosome is crucial for fertility. This study used FITC-PNA+PI to assess acrosome status in frozen-thawed stallion spermatozoa with varying motility levels. Results showed a strong correlation between LAI population and post-thaw sperm motility.
INDIAN JOURNAL OF ANIMAL SCIENCES
(2022)
Article
Genetics & Heredity
Alberto Ferrigno, Giovanni Ruvolo, Giuseppina Capra, Nicola Serra, Liana Bosco
Summary: The study demonstrated a strong correlation between DFI and sperm morphology, suggesting that spermatozoa with abnormal morphology are more likely to contain DNA damage.
JOURNAL OF ASSISTED REPRODUCTION AND GENETICS
(2021)
Article
Biochemical Research Methods
Hayley-Ann Bennett, Alyssa McAdorey, Hongbin Yan
Summary: The properties of six commonly used, commercially available, fluorescent dyes were compared in staining right-handed B-DNA and left-handed Z-DNA. All showed different degree of fluorescence turn-on in the presence of B-DNA, but very little in the presence of Z-DNA. The optimal range of dye-DNA ratios of DNA was determined. While these dyes do not provide a turn-on type probe for Z-DNA, staining between B- and Z-DNA using dyes such as SYBR Green I was shown to be useful in tracking the kinetics of conformational changes between these two forms of DNA. Finally, SYBR Green I showed unique circular dichroism patterns in 4 M NaCl that change in the presence of double stranded DNA, both in the visible and UV range.
JOURNAL OF FLUORESCENCE
(2023)
Review
Andrology
Rakesh Sharma, Concetta Iovine, Ashok Agarwal, Ralf Henkel
Summary: Sperm DNA integrity is crucial for normal reproductive functions, and sperm DNA fragmentation is common in infertile men. The TUNEL assay, which measures both single- and double-strand breaks in DNA, is becoming popular for assessing sperm DNA fragmentation. Various factors can affect the results, and challenges and clinical implications of TUNEL in male infertility evaluation are briefly discussed.
Article
Public, Environmental & Occupational Health
Dicky Faizal Irnandi, Aucky Hinting, Rina Yudiwati
Summary: This study found that chronic exposure to paint thinner among male workers in an automobile workshop in Malang increased the risk of DNA fragmentation in spermatozoa. Health education is recommended to minimize exposure risks for these workers.
TOXICOLOGY AND INDUSTRIAL HEALTH
(2021)
Review
Andrology
Andrea Crafa, Rosita Angela Condorelli, Sandro La Vignera, Aldo Eugenio Calogero, Rossella Cannarella
Summary: This article presents a clinical case of a globozoospermic patient with a new mutation of the DPY19L2 gene and provides recommendations based on a systematic literature review on gene mutations, assisted reproductive techniques, and the risk of genetic abnormalities transmission. The study highlights the importance of genetic evaluation and specific assisted reproductive techniques in managing globozoospermia patients.
WORLD JOURNAL OF MENS HEALTH
(2023)
Review
Andrology
Aldo E. Calogero, Rossella Cannarella, Ashok Agarwal, Taha Abo-Almagd Abdel-Meguid Hamoda, Amarnath Rambhatla, Ramadan Saleh, Florence Boitrelle, Imad Ziouziou, Tuncay Toprak, Murat Gul, Tomer Avidor-Reiss, Parviz Kavoussi, Eric Chung, Ponco Birowo, Ramy Abou Ghayda, Edmund Ko, Giovanni Colpi, Fotios Dimitriadis, Giorgio Ivan Russo, Marlon Martinez, Gokhan Calik, Hussein Kandil, Gianmaria Salvio, Taymour Mostafa, Haocheng Lin, Hyun Jun Park, Nazim Gherabi, Nguyen Ho Vinh Phuoc, Nguyen Quang, Ricky Adriansjah, Sandro La Vignera, Sava Micic, Damayanthi Durairajanayagam, Ege Can Serefoglu, Vilvapathy Senguttuvan Karthikeyan, Priyank Kothari, Widi Atmoko, Rupin Shah
Summary: Infertility affects nearly 186 million people worldwide, with male infertility accounting for about half of the cases. There has been a decline in sperm concentration and total count over the past four decades, emphasizing the need for further research in Andrology.
WORLD JOURNAL OF MENS HEALTH
(2023)
Article
Biochemistry & Molecular Biology
Rossella Cannarella, Francesca Mancuso, Nunziata Barone, Iva Arato, Cinzia Lilli, Catia Bellucci, Marco Musmeci, Giovanni Luca, Sandro La Vignera, Rosita A. Condorelli, Aldo E. Calogero
Summary: This study evaluated the expression of the follicle-stimulating hormone receptor (FSHR) in human spermatozoa and its effects on sperm function. The results showed that FSHR is expressed in the post-acrosomal segment, neck, midpiece, and tail of human spermatozoa, with no difference in localization between patients and controls. Incubation with FSH increased FSHR expression in patients. Furthermore, FSH incubation had a negative effect on sperm motility and mitochondrial function in both patients and controls, but more severely in controls. FSH also activated the AKT(473) signaling pathway.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Rossella Cannarella, Carmelo Gusmano, Rosita A. Condorelli, Andrea Bernini, Jurgen Kaftalli, Paolo Enrico Maltese, Stefano Paolacci, Astrit Dautaj, Giuseppe Marceddu, Matteo Bertelli, Sandro La Vignera, Aldo E. Calogero
Summary: This study investigated five unrelated patients with cHH/KS and identified a new pathogenic variant in the CHD7 gene and three new variants of unknown significance in the IL17RD, FGF17, and DUSP6 genes. Further functional studies are needed to confirm their association with cHH.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Biochemistry & Molecular Biology
Rossella Cannarella, Andrea Crafa, Federica Barbagallo, Scott D. D. Lundy, Sandro La Vignera, Rosita A. A. Condorelli, Aldo E. E. Calogero
Summary: This systematic review and meta-analysis examine the differences in H19 gene methylation between patients with abnormal versus normal sperm parameters. The study also explores the impact of age and sperm concentration on H19 methylation using meta-regression analysis. The results indicate that H19 methylation levels are lower in infertile patients, particularly those with oligozoospermia or recurrent pregnancy loss, and are independent of age and sperm concentration.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Medicine, General & Internal
Andrea Crafa, Aldo Eugenio Calogero, Rossella Cannarella, Rosita Angela Condorelli, Antonio Aversa, Sandro La Vignera
Summary: Male infertility and prostate cancer are the most researched andrological diseases, followed by benign prostatic hyperplasia and erectile dysfunction, which are expected to increase in prevalence due to population aging. However, research on inflammation of the accessory sexual glands is relatively scarce.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Medicine, General & Internal
Livia Basile, Rosita A. Condorelli, Aldo E. Calogero, Rossella Cannarella, Federica Barbagallo, Andrea Crafa, Antonio Aversa, Sandro La Vignera
Summary: Red wine is rich in nutrients and has been the focus of numerous scientific studies. It has been reported that moderate consumption of red wine, with its phenolic and antioxidant properties, can have positive effects on various diseases. Additionally, the ethanol content in red wine has shown various biological properties. However, little is known about the correlation between red wine consumption and male sexual function. This review aimed to evaluate the effects of moderate red wine consumption on erectile function and found potential benefits in patients with erectile dysfunction as well as positive influences on reproductive function through vasorelaxant and antioxidant mechanisms.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Medicine, General & Internal
Francesca Privitera, Danilo Centonze, Sandro La Vignera, Rosita Angela Condorelli, Costanza Distefano, Rossella Gioco, Alba Ilari Civit, Giordana Riccioli, Ludovica Stella, Andrea Cavallaro, Matteo Angelo Cannizzaro, Massimiliano Veroux
Summary: This study evaluated the incidence and potential risk factors for postoperative hypoparathyroidism after thyroid surgical procedures in a single high-volume center. The results showed that young patients undergoing neck dissection and with incidental parathyroidectomy have the highest risk of postoperative hypoparathyroidism.
JOURNAL OF CLINICAL MEDICINE
(2023)
Review
Biochemistry & Molecular Biology
Andrea Crafa, Rossella Cannarella, Federica Barbagallo, Claudia Leanza, Roberto Palazzolo, Hunter Ausley Flores, Sandro La Vignera, Rosita A. Condorelli, Aldo E. Calogero
Summary: Vitamin D deficiency and erectile dysfunction are common in males, especially in the elderly, suggesting a possible relationship between the two conditions. Studies indicate that vitamin D affects erectile function by regulating endothelial function and interfering with comorbidities associated with erectile dysfunction. Low levels of vitamin D may worsen erectile function through various mechanisms. However, the quality and heterogeneity of clinical trials evaluating the effects of vitamin D on erectile function and related comorbidities require further investigation.
Review
Biochemistry & Molecular Biology
Federica Barbagallo, Rossella Cannarella, Andrea Crafa, Sandro La Vignera, Rosita A. Condorelli, Claudio Manna, Aldo E. Calogero
Summary: This systematic review and meta-analysis found that a very short abstinence period (within 4 h) has a positive effect on assisted reproductive technique (ART) outcomes, significantly increasing the implantation rate, clinical pregnancy rate, and live birth rate. However, it does not have a significant impact on fertilization rate and miscarriage rate. It suggests that a second ejaculation shortly after the first one can be a simple strategy to improve ART outcomes, particularly in couples involving patients with abnormal sperm parameters.
Review
Pharmacology & Pharmacy
Rossella Cannarella, Cristina M. B. Petralia, Rosita A. Condorelli, Antonio Aversa, Aldo E. Calogero, Sandro La Vignera
Summary: This article provides an overview of the molecular mechanisms and usage of follicle-stimulating hormone (FSH) in the treatment of male infertility. It also evaluates the predictor factors of FSH administration response and the indications of the latest guidelines on the use of FSH in male infertility treatment.
EXPERT OPINION ON INVESTIGATIONAL DRUGS
(2023)
Article
Biochemistry & Molecular Biology
Alyssa J. J. Paganoni, Rossella Cannarella, Roberto Oleari, Federica Amoruso, Renata Antal, Marco Ruzza, Chiara Olivieri, Rosita A. Condorelli, Sandro La Vignera, Fationa Tolaj, Anna Cariboni, Aldo E. Calogero, Paolo Magni
Summary: Gonadotropin-releasing hormone (GnRH) neurons play a crucial role in regulating reproduction. AMH, GH, and IGF1 were found to enhance the migration and function of GnRH neurons in vitro. However, their involvement in the development and function of GnRH neurons in vivo remains unknown. This study evaluated the expression of AMHR2, GHR, and IGF1R during different developmental stages in mice, revealing differential protein expression patterns.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Genetics & Heredity
Andrea Crafa, Claudia Leanza, Rosita A. Condorelli, Sandro La Vignera, Aldo E. Calogero, Rossella Cannarella
Summary: This study suggests that the methylation degree of the LINE1 gene in sperm does not play a determining role in male infertility and/or abnormal sperm parameters. It also indicates that LINE1 should not be included in the genetic panel for identifying the most representative and cost-effective genes in couples undergoing ART cycles.
JOURNAL OF ASSISTED REPRODUCTION AND GENETICS
(2023)
Review
Andrology
Rossella Cannarella, Claudia Leanza, Andrea Crafa, Federica Barbagallo, Sandro La Vignera, Rosita Angela Condorelli, Aldo Eugenio Calogero
Summary: This study examined the impact of MEST gene methylation pattern on sperm parameters in infertile patients and found that MEST gene methylation was higher in infertile patients compared to normal men.
WORLD JOURNAL OF MENS HEALTH
(2023)
Article
Andrology
Rossella Cannarella, Matteo Bertelli, Rosita A. Condorelli, Marija Vilaj, Sandro La Vignera, Davor Jezek, Aldo E. Calogero
Summary: This study revealed that different mutations of the same gene can result in various testicular histological pictures. It suggests that it is the type of gene mutation/variation rather than the gene itself that determines the testicular histology. However, designing a genetic panel with prognostic value for testicular sperm extraction in patients with NOA remains challenging.
WORLD JOURNAL OF MENS HEALTH
(2023)
