Article
Veterinary Sciences
Effrosyni Fatira, Milos Havelka, Taiju Saito, Jose Landeira, Marek Rodina, David Gela, Martin Psenicka
Summary: In this study, the researchers applied intracytoplasmic sperm injection (ICSI) technology to endangered sturgeons and found that it successfully produced embryos and larvae. Furthermore, molecular analysis showed genome integration of both egg and sperm donor species, demonstrating the potential of ICSI for assisted reproduction in sturgeons.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Multidisciplinary Sciences
Madara S. B. Ralapanawe, Sugandika L. Gajaweera, Nishendra Karunaratne, Vajira H. W. Dissnayake, Malcolm J. Price, Pedro Melo, Arri Coomarasamy, Ioannis D. Gallos
Summary: Recent studies have suggested an association between chromosomal polymorphic variations and infertility, with higher rates of miscarriage found in assisted reproduction. This study aims to analyze the influence of specific types or number of chromosomal polymorphic variations on reproductive outcomes in couples undergoing ICSI treatment. The analysis of data from 929 fresh and frozen embryo transfer cycles of 692 women revealed no significant difference in reproductive outcomes based on carriers or non-carriers of chromosomal polymorphic variations. Further studies are needed to determine whether detecting these variations before assisted conception is a useful diagnostic tool.
SCIENTIFIC REPORTS
(2023)
Review
Medicine, General & Internal
Sandrine Chamayou, Filippo Giacone, Rossella Cannarella, Antonino Guglielmino
Summary: The intracytoplasmic sperm injection (ICSI) technique was invented to address severe male infertility caused by altered sperm parameters. It is now widely used worldwide for couple infertility treatment. ICSI can be performed using any available spermatozoon, regardless of motility, morphology, or quantity. Published data shows that ICSI accelerates zygote kinetics and leads to higher fertilization rates and more blastocysts for clinical use compared to conventional IVF. Various sperm and spermatozoa characteristics, such as parameters, morphology, vitality, DNA content, RNA content, epigenetics, and recovery site, have different impacts on fertilization, blastocyst rates, and embryo kinetics. While ICSI is the most common solution for couple infertility, understanding the causes of male infertility is crucial for producing competent spermatozoa that contribute to normal embryonic development and healthy offspring.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Endocrinology & Metabolism
Yubin Li, Qiyuan Chang, Qingyun Mai
Summary: This retrospective cohort study investigated the incidence, pregnancy and neonatal outcomes of monozygotic twins (MZTs) following assisted reproductive technology (ART) treatments, including IVF, ICSI, PGT, and TESA. The study found that the rate of MZTs was similar among the four ART groups, but the IVF group had a higher risk of pregnancy loss and early miscarriage, while the TESA group had a higher risk of twin-to-twin transfusion syndrome (TTTS).
REPRODUCTIVE BIOLOGY AND ENDOCRINOLOGY
(2023)
Article
Obstetrics & Gynecology
Adva Aizer, Alon Lazarovich, Olga Dratviman-Storobinsky, Meirav Noach-Hirsh, Jigal Haas, Avi Jacob, Gil Raviv, Raoul Orvieto
Summary: The study found that in ICSI cycles, using motile spermatozoa obtained from TESA or TESE resulted in higher pregnancy and live birth rates compared to using immotile spermatozoa. While fertilization rates and embryo quality decreased with the use of immotile spermatozoa, ICSI remained a valid option for couples.
REPRODUCTIVE BIOMEDICINE ONLINE
(2021)
Review
Genetics & Heredity
Veena Unnikrishnan, John Kastelic, Jacob Thundathil
Summary: In cattle, the efficiency of ICSI is hindered by failed oocyte activation post sperm injection. Research focusing on proteins like Na/K-ATPase and PLC zeta is key to improving the success rate. Enhancing the efficiency of bovine ICSI has significant implications for the cattle breeding industry.
Review
Agriculture, Dairy & Animal Science
Achraf Benammar, Emilie Derisoud, Francois Vialard, Eric Palmer, Jean Marc Ayoubi, Marine Poulain, Pascale Chavatte-Palmer
Summary: Horses serve as a potential animal model for researching artificial reproduction techniques, despite significant differences in reproductive anatomy and processes with humans. However, similarities are found in embryo development and certain reproductive phenomena. Both species share common concerns related to infertility.
Article
Biochemical Research Methods
Sa Xiao, Jason Riordon, Mohammad Simchi, Alexander Lagunov, Thomas Hannam, Keith Jarvi, Reza Nosrati, David Sinton
Summary: FertDish is a new tool for assisted reproduction technology that allows for rapid selection of high quality sperm, resulting in improvements in DNA fragmentation index, viability, and distribution of sperm with high DNA levels. It is faster, more convenient, and easily adaptable to the clinical workflow compared to traditional methods.
Review
Medicine, General & Internal
Lauren Lacey, Sibte Hassan, Sebastian Franik, Mourad W. Seif, M. Ahsan Akhtar
Summary: The effects of assisted hatching (AH) on live birth rates are uncertain and may increase the risk of multiple pregnancies. Although AH may offer a slightly increased chance of achieving a clinical pregnancy, the quality of data is low, and it is uncertain whether it influences miscarriage rates.
COCHRANE DATABASE OF SYSTEMATIC REVIEWS
(2021)
Article
Medicine, General & Internal
Huixiao Chen, Ping Sun, Na Zhang, Shangge Lv, Yongzhi Cao, Lei Yan
Summary: This study assessed the effect of uterine septum resection on reproductive outcomes in patients with secondary infertility undergoing in vitro fertilization/intracytoplasmic sperm injection. The results showed that uterine septum resection improved the pregnancy rates and live birth rates in these patients.
FRONTIERS IN MEDICINE
(2022)
Article
Reproductive Biology
Lachlan G. Howell, Ryan R. Witt
Summary: Assisted reproductive technologies (ARTs) have the potential to contribute significantly to the conservation of endangered wildlife, such as the koala. However, the lack of funding hinders the application of ARTs in koalas and other wildlife. It is important to raise public awareness and secure funding for the development of ARTs.
Review
Genetics & Heredity
Marziyeh Mazaheri Moghaddam, Madiheh Mazaheri Moghaddam, Hamid Hamzeiy, Amir Baghbanzadeh, Fariba Pashazadeh, Ebrahim Sakhinia
Summary: This study found that acephalic spermatozoa syndrome has a genetic origin, and several genetic variations related to the pathogenesis of this anomaly have been identified. ICSI may provide a promising treatment for acephalic spermatozoa-associated male infertility. However, in addition to the importance of sperm head-tail junction integrity, other factors, whether within the sperm cell or female factors, may also be involved in the outcome of ICSI.
JOURNAL OF ASSISTED REPRODUCTION AND GENETICS
(2021)
Article
Obstetrics & Gynecology
Ahmad Aboukhshaba, Nahid Punjani, Sofia Doukakis, Nikica Zaninovic, Gianpiero Palermo, Peter N. Schlegel
Summary: The study aimed to evaluate the impact of sperm characteristics on intracytoplasmic sperm injection (ICSI) outcomes in men with nonobstructive azoospermia (NOA) following microdissection testicular sperm extraction (mTESE). The results showed that specific sperm defects did not completely preclude the chance of clinical pregnancy. This study is the first comprehensive evaluation of the importance of these characteristics in ICSI outcomes.
FERTILITY AND STERILITY
(2022)
Article
Andrology
Lin Huang, Guanping Yao, Gelin Huang, Chuan Jiang, Li Li, Lu Liao, Guiping Yuan, Lijun Shang, Wenming Xu
Summary: High levels of DNA damage and low levels of Zinc and SOD activities in semen of globozoospermic infertile patients may be responsible for the failure of ICSI in these patients.
TRANSLATIONAL ANDROLOGY AND UROLOGY
(2021)
Article
Obstetrics & Gynecology
Yukiko Katagiri, Yuko Tamaki
Summary: Reproductive medicine requires genetic knowledge, including understanding of birth defects, chromosomal abnormalities, Y chromosome microdeletions, chromosomal abnormalities in oligospermia, and epigenetic alterations. Professionals in this field must be well-versed in these areas for successful treatment.
REPRODUCTIVE MEDICINE AND BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Rossella Cannarella, Francesca Mancuso, Nunziata Barone, Iva Arato, Cinzia Lilli, Catia Bellucci, Marco Musmeci, Giovanni Luca, Sandro La Vignera, Rosita A. Condorelli, Aldo E. Calogero
Summary: This study evaluated the expression of the follicle-stimulating hormone receptor (FSHR) in human spermatozoa and its effects on sperm function. The results showed that FSHR is expressed in the post-acrosomal segment, neck, midpiece, and tail of human spermatozoa, with no difference in localization between patients and controls. Incubation with FSH increased FSHR expression in patients. Furthermore, FSH incubation had a negative effect on sperm motility and mitochondrial function in both patients and controls, but more severely in controls. FSH also activated the AKT(473) signaling pathway.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Rossella Cannarella, Carmelo Gusmano, Rosita A. Condorelli, Andrea Bernini, Jurgen Kaftalli, Paolo Enrico Maltese, Stefano Paolacci, Astrit Dautaj, Giuseppe Marceddu, Matteo Bertelli, Sandro La Vignera, Aldo E. Calogero
Summary: This study investigated five unrelated patients with cHH/KS and identified a new pathogenic variant in the CHD7 gene and three new variants of unknown significance in the IL17RD, FGF17, and DUSP6 genes. Further functional studies are needed to confirm their association with cHH.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Biochemistry & Molecular Biology
Rossella Cannarella, Andrea Crafa, Federica Barbagallo, Scott D. D. Lundy, Sandro La Vignera, Rosita A. A. Condorelli, Aldo E. E. Calogero
Summary: This systematic review and meta-analysis examine the differences in H19 gene methylation between patients with abnormal versus normal sperm parameters. The study also explores the impact of age and sperm concentration on H19 methylation using meta-regression analysis. The results indicate that H19 methylation levels are lower in infertile patients, particularly those with oligozoospermia or recurrent pregnancy loss, and are independent of age and sperm concentration.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Medicine, General & Internal
Andrea Crafa, Aldo Eugenio Calogero, Rossella Cannarella, Rosita Angela Condorelli, Antonio Aversa, Sandro La Vignera
Summary: Male infertility and prostate cancer are the most researched andrological diseases, followed by benign prostatic hyperplasia and erectile dysfunction, which are expected to increase in prevalence due to population aging. However, research on inflammation of the accessory sexual glands is relatively scarce.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Medicine, General & Internal
Livia Basile, Rosita A. Condorelli, Aldo E. Calogero, Rossella Cannarella, Federica Barbagallo, Andrea Crafa, Antonio Aversa, Sandro La Vignera
Summary: Red wine is rich in nutrients and has been the focus of numerous scientific studies. It has been reported that moderate consumption of red wine, with its phenolic and antioxidant properties, can have positive effects on various diseases. Additionally, the ethanol content in red wine has shown various biological properties. However, little is known about the correlation between red wine consumption and male sexual function. This review aimed to evaluate the effects of moderate red wine consumption on erectile function and found potential benefits in patients with erectile dysfunction as well as positive influences on reproductive function through vasorelaxant and antioxidant mechanisms.
JOURNAL OF CLINICAL MEDICINE
(2023)
Review
Biochemistry & Molecular Biology
Andrea Crafa, Rossella Cannarella, Federica Barbagallo, Claudia Leanza, Roberto Palazzolo, Hunter Ausley Flores, Sandro La Vignera, Rosita A. Condorelli, Aldo E. Calogero
Summary: Vitamin D deficiency and erectile dysfunction are common in males, especially in the elderly, suggesting a possible relationship between the two conditions. Studies indicate that vitamin D affects erectile function by regulating endothelial function and interfering with comorbidities associated with erectile dysfunction. Low levels of vitamin D may worsen erectile function through various mechanisms. However, the quality and heterogeneity of clinical trials evaluating the effects of vitamin D on erectile function and related comorbidities require further investigation.
Review
Biochemistry & Molecular Biology
Federica Barbagallo, Rossella Cannarella, Andrea Crafa, Sandro La Vignera, Rosita A. Condorelli, Claudio Manna, Aldo E. Calogero
Summary: This systematic review and meta-analysis found that a very short abstinence period (within 4 h) has a positive effect on assisted reproductive technique (ART) outcomes, significantly increasing the implantation rate, clinical pregnancy rate, and live birth rate. However, it does not have a significant impact on fertilization rate and miscarriage rate. It suggests that a second ejaculation shortly after the first one can be a simple strategy to improve ART outcomes, particularly in couples involving patients with abnormal sperm parameters.
Review
Pharmacology & Pharmacy
Rossella Cannarella, Cristina M. B. Petralia, Rosita A. Condorelli, Antonio Aversa, Aldo E. Calogero, Sandro La Vignera
Summary: This article provides an overview of the molecular mechanisms and usage of follicle-stimulating hormone (FSH) in the treatment of male infertility. It also evaluates the predictor factors of FSH administration response and the indications of the latest guidelines on the use of FSH in male infertility treatment.
EXPERT OPINION ON INVESTIGATIONAL DRUGS
(2023)
Article
Biochemistry & Molecular Biology
Alyssa J. J. Paganoni, Rossella Cannarella, Roberto Oleari, Federica Amoruso, Renata Antal, Marco Ruzza, Chiara Olivieri, Rosita A. Condorelli, Sandro La Vignera, Fationa Tolaj, Anna Cariboni, Aldo E. Calogero, Paolo Magni
Summary: Gonadotropin-releasing hormone (GnRH) neurons play a crucial role in regulating reproduction. AMH, GH, and IGF1 were found to enhance the migration and function of GnRH neurons in vitro. However, their involvement in the development and function of GnRH neurons in vivo remains unknown. This study evaluated the expression of AMHR2, GHR, and IGF1R during different developmental stages in mice, revealing differential protein expression patterns.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Genetics & Heredity
Andrea Crafa, Claudia Leanza, Rosita A. Condorelli, Sandro La Vignera, Aldo E. Calogero, Rossella Cannarella
Summary: This study suggests that the methylation degree of the LINE1 gene in sperm does not play a determining role in male infertility and/or abnormal sperm parameters. It also indicates that LINE1 should not be included in the genetic panel for identifying the most representative and cost-effective genes in couples undergoing ART cycles.
JOURNAL OF ASSISTED REPRODUCTION AND GENETICS
(2023)
Article
Andrology
Rossella Cannarella, Matteo Bertelli, Rosita A. Condorelli, Marija Vilaj, Sandro La Vignera, Davor Jezek, Aldo E. Calogero
Summary: This study revealed that different mutations of the same gene can result in various testicular histological pictures. It suggests that it is the type of gene mutation/variation rather than the gene itself that determines the testicular histology. However, designing a genetic panel with prognostic value for testicular sperm extraction in patients with NOA remains challenging.
WORLD JOURNAL OF MENS HEALTH
(2023)