期刊
NUCLEIC ACIDS RESEARCH
卷 42, 期 W1, 页码 W408-W415出版社
OXFORD UNIV PRESS
DOI: 10.1093/nar/gku428
关键词
-
资金
- Novo Nordisk Foundation
- National Institutes of Health Director's Early Independence Award [1DP5OD009172-01]
- Lundbeck Foundation [R140-2013-13496, R77-2010-6772] Funding Source: researchfish
- Novo Nordisk Fonden [NNF13SA0006019, NNF14OC0011335, NNF10CC1016517, NNF13SA0009311] Funding Source: researchfish
Recombineering and multiplex automated genome engineering (MAGE) offer the possibility to rapidly modify multiple genomic or plasmid sites at high efficiencies. This enables efficient creation of genetic variants including both single mutants with specifically targeted modifications as well as combinatorial cell libraries. Manual design of oligonucleotides for these approaches can be tedious, time-consuming, and may not be practical for larger projects targeting many genomic sites. At present, the change from a desired phenotype (e.g. altered expression of a specific protein) to a designed MAGE oligo, which confers the corresponding genetic change, is performed manually. To address these challenges, we have developed the MAGE Oligo Design Tool (MODEST). This web-based tool allows designing of MAGE oligos for (i) tuning translation rates by modifying the ribosomal binding site, (ii) generating translational gene knockouts and (iii) introducing other coding or non-coding mutations, including amino acid substitutions, insertions, deletions and point mutations. The tool automatically designs oligos based on desired genotypic or phenotypic changes defined by the user, which can be used for high efficiency recombineering and MAGE.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据