Article
Microbiology
Simran Preet Bhalla, Ann Maria Shaju, Carlos Marcelo da Silva Figueredo, Leticia Algarves Miranda
Summary: C5a levels were significantly increased in both gingival crevicular fluid and saliva of patients with periodontal disease, indicating a potential role of this molecule in the pathogenesis of the disease.
Article
Dentistry, Oral Surgery & Medicine
Hyun-Duck Kim, Sandeep Karna, YooJin Shin, Huong Vu, Hyun-Jae Cho, Sungtae Kim
Summary: This study compared the association and screening ability of S100A8 and S100A9 in saliva, blood, and gingival crevicular fluid for periodontitis status. Salivary S100A8 was significantly higher in established periodontitis, while GCF S100A8 and S100A9 were higher in no or initial periodontitis. Salivary S100A8 could be a potential diagnostic marker for established periodontitis.
Article
Dentistry, Oral Surgery & Medicine
Melissa M. Grant, John J. Taylor, Katrin Jaedicke, Andrew Creese, Catherine Gowland, Bernard Burke, Khawla Doudin, Upen Patel, Paul Weston, Michael Milward, Susan M. Bissett, Helen J. Cooper, Gerben Kooijman, Amir Rmaile, Marko de Jager, Philip M. Preshaw, Iain L. C. Chapple
Summary: Differential protein biomarkers in saliva and gingival crevicular fluid can be used to objectively distinguish between periodontal health and disease states.
JOURNAL OF CLINICAL PERIODONTOLOGY
(2022)
Article
Multidisciplinary Sciences
Mayra A. Tellez Corral, Eddy Herrera Daza, Natalia Arango Jimenez, Darena Z. Morales Vera, Juliana Velosa Porras, Catalina Latorre Uriza, Francina M. Escobar Arregoces, Patricia Hidalgo Martinez, Maria E. Cortes, Liliana Otero, Claudia M. Parra Giraldo, Nelly S. Roa Molina
Summary: This study compares the concentrations of five cytokines in saliva and gingival crevicular fluid (GCF) in patients with OSA and investigates their association with periodontitis. Samples were obtained from 84 patients classified into four groups. The results show that severe OSA patients have the highest proportion of stage III periodontitis. The cytokine profile in saliva and GCF differs between different groups, suggesting an increased risk of developing periodontitis in patients with OSA. These cytokines could serve as biomarkers for OSA and periodontitis.
Article
Multidisciplinary Sciences
Takumi Hiyoshi, Hisanori Domon, Tomoki Maekawa, Hikaru Tamura, Toshihito Isono, Satoru Hirayama, Karin Sasagawa, Fumio Takizawa, Koichi Tabeta, Yutaka Terao
Summary: This study reveals the mechanisms by which neutrophil elastase (NE) exacerbates periodontitis. NE induces increased neutrophil recruitment and activity in the periodontal tissue, leading to worsened periodontal bone loss. Administration of an NE inhibitor reduces NE activity and attenuates periodontal bone loss. NE also promotes bacterial invasion in periodontal tissues by cleaving cell adhesion molecules and disrupting the gingival epithelial barrier.
SCIENTIFIC REPORTS
(2022)
Article
Biochemistry & Molecular Biology
Yong Liu, Renping Zhao, Bashar Reda, Wenjuan Yang, Matthias Hannig, Bin Qu
Summary: This study analyzed the cytokines, chemokines, and growth factors in saliva and GCF, revealing that profiles of cytokines, chemokines, and growth factors are individualized for different donors in saliva, while profiles of cytokines and chemokines are similar in GCF.
Review
Biochemistry & Molecular Biology
Ionut Luchian, Ancuta Goriuc, Darius Sandu, Mihai Covasa
Summary: Severe periodontitis is a destructive inflammatory disease that affects 11% of the global population. Metalloproteinases play a crucial role in cellular development and are associated with periodontal diseases. MMP-8 and MMP-9 are valuable diagnostic tools for periodontitis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Dentistry, Oral Surgery & Medicine
Pingping Han, Corey S. Moran, Srinivas Sulugodu Ramachandra, Laurence J. Walsh, Saso Ivanovski
Summary: This prospective cohort study aimed to evaluate antibody responses in gingival crevicular fluid (GCF) and saliva to the SARS-CoV-2 Spike protein following vaccination. The study found that antibody levels peaked at 3 weeks after vaccination, and the concentration of antibodies in GCF was significantly higher than in saliva.
JOURNAL OF PERIODONTOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Javier Fernandez, Constanza Jimenez, Dafna Benadof, Paulina Morales, Jessica Astorga, Felipe Caceres, Marcela Hernandez, Alejandra Fernandez, Fernando Valenzuela
Summary: The study aimed to evaluate the levels of MMP-2 and MMP-9 in gingival crevicular fluid as novel biomarkers for rosacea. The results showed a significant increase in MMP-9 concentration in the GCF of patients with rosacea compared to healthy patients. MMP-9 levels can be used as a diagnostic biomarker for rosacea.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Dentistry, Oral Surgery & Medicine
Dax Abraham, Arundeep Singh, Anjana Goyal
Summary: This study compared the levels of NLRP3 protein in the saliva and gingival crevicular fluid of patients with symptomatic irreversible pulpitis and healthy controls. The results showed significantly higher levels of NLRP3 in the saliva and GCF of SIP patients compared to healthy controls, indicating its potential as a molecular diagnostic biomarker for pulpal disorders.
JOURNAL OF ENDODONTICS
(2023)
Article
Dentistry, Oral Surgery & Medicine
Zeynep Pinar Keles Yucel, Beral Afacan, Harika Atmaca Ilhan, Timur Kose, Gulnur Emingil
Summary: This study found elevated levels of TFF-1, TFF-3, and IL-1 beta in periodontitis patients compared to healthy controls and gingivitis group across GCF, saliva, and serum samples. These findings suggest the involvement of these peptides in host response during periodontal tissue destruction.
Article
Dentistry, Oral Surgery & Medicine
Nil Yakar, Gulnur Emingil, Asena Turedi, Cagdas Sahi, Timur Kose, Nagihan Bostanci, Angelika Silbereisen
Summary: This study aimed to investigate the association of GCF TREM-1, PGLYRP1, and IL-1 β levels with periodontal health in pre- and postmenopausal women. The results showed that there were no significant differences in GCF levels of TREM-1, PGLYRP1, and IL-1 β between pre- and postmenopausal women. However, higher GCF TREM-1 levels were observed in women with periodontitis, indicating that TREM-1 may be an indicator for periodontitis in both pre- and postmenopausal women.
JOURNAL OF PERIODONTAL RESEARCH
(2023)
Review
Peripheral Vascular Disease
Aleksandra Szustkiewicz-Karon, Martyna Schonborn, Patrycja Pasieka, Anna Plotek, Pawel Maga, Iwona D. Gregorczyk-Maga
Summary: This review summarizes the role of saliva and gingival crevicular fluid (GCF) in screening and early diagnosis of cardiovascular diseases (CVDs). The findings suggest that substances in saliva, including inflammatory markers, enzymes, and hormones, may serve as novel biomarkers for diagnosing and screening CVDs.
Article
Dentistry, Oral Surgery & Medicine
V. Ozgen Ozturk, Pinar Meric, Timo Sorsa, Taina Tervahartiala, Nagihan Bostanci, Solomon O. Nwhator, Gulnur Emingil
Summary: This study investigated the levels of MMPs and TIMPs in the saliva and GCF of pregnant women during the second and third trimester of pregnancy and postpartum. The results showed higher levels of MMP-8 and MMP-9 in the saliva of pregnant women, along with lower levels of TIMP-1, indicating increased inflammatory burden during pregnancy. These findings can be used for monitoring the inflammatory state of gingival tissues during pregnancy.
ARCHIVES OF ORAL BIOLOGY
(2021)
Article
Dentistry, Oral Surgery & Medicine
Sheila L. Arvikar, Hatice Hasturk, Klemen Strle, Danielle Stephens, Marcy B. Bolster, Deborah S. Collier, Alpdogan Kantarci, Allen C. Steere
Summary: Despite receiving routine dental care, RA patients often show inflammation in oral fluids, with inflammatory profiles differing from serum and joints. Neutrophilic inflammatory profiles in oral fluids suggest that gingival tissues are a common and often unrecognized site of extra-articular inflammation in RA.
JOURNAL OF PERIODONTOLOGY
(2021)
Article
Dentistry, Oral Surgery & Medicine
Tomoyuki Iwata, Noriyoshi Mizuno, Takayoshi Nagahara, Eri Kaneda-Ikeda, Mikihito Kajiya, Masae Kitagawa, Katsuhiro Takeda, Minami Yoshioka, Ryoichi Yagi, Takashi Takata, Hidemi Kurihara
Summary: The study aims to identify regulatory genes involved in periodontal cell differentiation and clarify the differentiation mechanism for effective periodontal regenerative therapy.
JOURNAL OF PERIODONTAL RESEARCH
(2021)
Editorial Material
Biochemistry & Molecular Biology
Mikihito Kajiya, Hidemi Kurihara
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Saki Hatano, Shinji Matsuda, Ai Okanobu, Daisuke Furutama, Takumi Memida, Mikihito Kajiya, Kazuhisa Ouhara, Tsuyoshi Fujita, Noriyoshi Mizuno, Hidemi Kurihara
Summary: The study revealed the significant role of NR4A1 in drug-induced gingival overgrowth, with its agonist inhibiting growth development. Additionally, NFAT and calcium levels were found to be related to this process.
Article
Dentistry, Oral Surgery & Medicine
Tomoyuki Iwata, Noriyoshi Mizuno, Takayoshi Nagahara, Eri Kaneda-Ikeda, Mikihito Kajiya, Shinya Sasaki, Katsuhiro Takeda, Mari Kiyota, Ryoichi Yagi, Tsuyoshi Fujita, Hidemi Kurihara
Summary: This study investigated the regulatory genes induced by periodontal regenerative cytokines using MSCs, identifying miR-628-5p as a negative regulator of MSC stemness during periodontal regeneration. Periodontal regenerative cytokines act as suppressors of miR-628-5p to support periodontal regeneration, indicating the importance of selecting effective cytokines for different MSCs based on miRNA profiling in advancing regenerative therapies.
JOURNAL OF PERIODONTOLOGY
(2022)
Article
Cell & Tissue Engineering
Tomoya Ogawa, Mikihito Kajiya, Susumu Horikoshi, Hiroki Yoshii, Mai Yoshino, Souta Motoike, Shin Morimoto, Hisakatsu Sone, Tomoyuki Iwata, Kazuhisa Ouhara, Shinji Matsuda, Noriyoshi Mizuno
Summary: The study suggests that MSCs treated with C-MSCsy can retain their immunosuppressive and osteogenic properties after cryopreservation, providing a promising off-the-shelf cell preparation for bone regenerative therapy.
REGENERATIVE THERAPY
(2022)
Article
Cell & Tissue Engineering
Hisakatsu Sone, Mikihito Kajiya, Katsuhiro Takeda, Shinya Sasaki, Susumu Horikoshi, Souta Motoike, Shin Morimoto, Hiroki Yoshii, Mai Yoshino, Tomoyuki Iwata, Kazuhisa Ouhara, Shinji Matsuda, Noriyoshi Mizuno
Summary: Periodontal tissue regeneration can be achieved by transplanting clumps of mesenchymal stem cells (MSCs) and extracellular matrix (ECM) complexes (C-MSCs) without artificial scaffold. In a rat model, C-MSCs transplantation successfully induced periodontal tissue regeneration, with the donor cells directly involved in tissue formation. This study demonstrates a promising scaffold-free MSCs transplantation strategy for periodontal disease.
JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE
(2022)
Article
Cell & Tissue Engineering
Daisuke Kamiya, Nana Takenaka-Ninagawa, Souta Motoike, Mikihito Kajiya, Teppei Akaboshi, Chengzhu Zhao, Mitsuaki Shibata, Sho Senda, Yayoi Toyooka, Hidetoshi Sakurai, Hidemi Kurihara, Makoto Ikeya
Summary: In this study, human induced pluripotent stem cells (iPSCs) were successfully induced into mesenchymal stem cells (MSCs) under xeno-free conditions. The xeno-free-induced MSCs (XF-iMSCs) were found to have regenerative potential in bone and skeletal muscle regeneration. They promoted the regeneration of surrounding host cells through the secretion of soluble factors, and the secretion of peroxidasin and IGF2 also contributed to myotube differentiation.
NPJ REGENERATIVE MEDICINE
(2022)
Review
Health Care Sciences & Services
Toshinori Ando, Kento Okamoto, Tomoaki Shintani, Souichi Yanamoto, Mutsumi Miyauchi, J. Silvio Gutkind, Mikihito Kajiya
Summary: Genetic alterations and dysregulation of signaling pathways are crucial for the development of cancer. Precision medicine allows for the selection of appropriate treatments based on genetic variations. Various genetic alterations in HNSCC disrupt the Hippo pathway and lead to hyperactivation of YAP/TAZ.
JOURNAL OF PERSONALIZED MEDICINE
(2022)
Article
Dentistry, Oral Surgery & Medicine
Tomoyuki Iwata, Eri Kaneda-Ikeda, Keita Takahashi, Katsuhiro Takeda, Takayoshi Nagahara, Mikihito Kajiya, Shinya Sasaki, Shu Ishida, Minami Yoshioka, Shinji Matsuda, Kazuhisa Ouhara, Tsuyoshi Fujita, Hidemi Kurihara, Noriyoshi Mizuno
Summary: This study aimed to investigate the regulatory mechanism of bone marrow-derived mesenchymal stem cell (BM-MSC) differentiation by humoral factors derived from human periodontal ligament (HPL) cells and human gingival fibroblasts (HGFs). The results showed that histone deacetylase (HDAC) expression and histone H3 acetylation were involved in the regulation of BM-MSC differentiation. In clinical applications, regulation of HDAC expression holds potential for effective periodontal regeneration, particularly bone regeneration.
JOURNAL OF PERIODONTAL RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Tetsuya Tamura, Ruoqi Zhai, Tasuku Takemura, Kazuhisa Ouhara, Yuri Taniguchi, Yuta Hamamoto, Ryousuke Fujimori, Mikihito Kajiya, Shinji Matsuda, Syuichi Munenaga, Tsuyoshi Fujita, Noriyoshi Mizuno
Summary: The study demonstrates that geniposidic acid (GPA) has anti-inflammatory effects and can inhibit Porphyromonas gingivalis-induced periodontitis, suggesting its potential in preventing periodontal disease.
Article
Dentistry, Oral Surgery & Medicine
Tatsuya Akitomo, Yuria Asao, Yuko Iwamoto, Satoru Kusaka, Momoko Usuda, Mariko Kametani, Toshinori Ando, Shinnichi Sakamoto, Chieko Mitsuhata, Mikihito Kajiya, Katsuyuki Kozai, Ryota Nomura
Summary: A 4-year-and-11-month-old boy had three supernumerary teeth, which required surgical treatment and long-term follow-up. The first supernumerary tooth was extracted at 5 years and 5 months, followed by two more at 7 years and 7 months and 8 years and 2 months. Malocclusion may be caused by the supernumerary teeth, and surgical exposure was performed to facilitate the eruption of the permanent teeth.
Article
Dentistry, Oral Surgery & Medicine
Fumitaka Obayashi, Koichi Koizumi, Nanako Ito, Nami Obayashi, Tomoaki Shintani, Mikihito Kajiya, Souichi Yanamoto
Summary: Palmoplantar pustulosis (PPP) is a stubborn skin disease characterized by small aseptic pustules on the palms and soles, which can worsen due to metal and dental focal infections. This study reports a case of PPP exacerbation after orthognathic surgery, attributing it to metal allergy from the plates or screws used.
Article
Cell & Tissue Engineering
Shin Morimoto, Mikihito Kajiya, Hiroki Yoshii, Mai Yoshino, Susumu Horikoshi, Souta Motoike, Tomoyuki Iwata, Kazuhisa Ouhara, Toshinori Ando, Tetsuya Yoshimoto, Tomoaki Shintani, Noriyoshi Mizuno
Summary: Three-dimensional clumps of MSCs/ECM complexes (C-MSCs) can be implanted without artificial scaffolds, and their cellular properties can be regulated in vitro. The study aimed to generate cartilaginous tissue covered with a mineralized bone collar-like structure from human C-MSCs by combining chondrogenic and osteogenic induction, showing potential for bone-regenerative cell therapy.
STEM CELL REVIEWS AND REPORTS
(2023)
Article
Health Care Sciences & Services
Hiromi Nishi, Susumu Horikoshi, Kouji Ohta, Tetsumi Yoshida, Noriyasu Fukushima, Kyoko Oshita, Syuichi Munenaga, Taro Edahiro, Hiroshi Ureshino, Hideo Shigeishi, Yukio Yoshioka, Masaru Konishi, Noriaki Ide, Yuma Ogawa, Rikou Marukawa, Tomoaki Shintani, Natumi Ino, Mikihito Kajiya, Naoya Kakimoto, Hiroki Ohge, Tatsuo Ichinohe, Hiroyuki Kawaguchi
Summary: LLLT has shown significant efficacy in preventing severe oral mucositis among hematologic cancer patients in Japan, leading to a lower incidence rate. This study fills the gap in clinical adoption of LLLT in Japan.
JOURNAL OF PERSONALIZED MEDICINE
(2023)
Article
Cell & Tissue Engineering
Hiroki Yoshii, Mikihito Kajiya, Mai Yoshino, Shin Morimoto, Susumu Horikoshi, Misako Tari, Souta Motoike, Tomoyuki Iwata, Kazuhisa Ouhara, Toshinori Ando, Tetsuya Yoshimoto, Tomoaki Shintani, Noriyoshi Mizuno
Summary: This study investigates the role of mechanotransducer YAP/TAZ and their binding target transcriptional factor, TEAD, in the immunomodulatory capacities of human bone marrow-derived MSCs. The results show that disrupting the YAP/TAZ-TEAD axis can enhance the immunomodulatory properties of MSCs.
STEM CELL REVIEWS AND REPORTS
(2023)