Article
Dentistry, Oral Surgery & Medicine
Tomoaki Iwayama, Hiromi Sakashita, Masahide Takedachi, Shinya Murakami
Summary: This review summarizes the recent findings on tissue stem cells in the periodontal ligament (PDL) and discusses future research directions for periodontal regenerative therapy.
JAPANESE DENTAL SCIENCE REVIEW
(2022)
Review
Biochemistry & Molecular Biology
Mariacristina Amato, Simona Santonocito, Gaia Viglianisi, Marco Tatullo, Gaetano Isola
Summary: Periodontal disease is a common chronic inflammatory condition that lacks effective treatment options. Utilizing stem cells for periodontal tissue regeneration shows promise as a potential therapeutic approach.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Dentistry, Oral Surgery & Medicine
Shengnan Li, Xiuchen Guan, Wenting Yu, Zeqing Zhao, Yaxi Sun, Yuxing Bai
Summary: This study aimed to investigate the effect of exosomes derived from human periodontal ligament stem cells on the activity of cementoblasts. The results showed that exosomes can promote the migration, proliferation, and mineralization of cementoblasts, and this effect is mediated through the PI3K/AKT signaling pathway. This provides a scientific basis for promoting the repair process in orthodontically induced inflammatory root resorption.
Article
Biochemistry & Molecular Biology
Orie Adachi, Hideki Sugii, Tomohiro Itoyama, Shoko Fujino, Hiroshi Kaneko, Atsushi Tomokiyo, Sayuri Hamano, Daigaku Hasegawa, Junko Obata, Shinichiro Yoshida, Masataka Kadowaki, Risa Sugiura, Mhd Safwan Albougha, Hidefumi Maeda
Summary: The study aims to clarify the biological functions of decorin in the healing and regeneration of wounded periodontal tissue. The results demonstrate that decorin promotes osteoblastic differentiation of periodontal ligament stem cells and contributes to bone healing through ERK1/2 signaling pathway.
Article
Cell & Tissue Engineering
Liya Ma, Nanquan Rao, Hui Jiang, Yuzhe Dai, Songtao Yang, Hefeng Yang, Jiangtian Hu
Summary: This study investigates the potential of DFSCs-sEVs in regenerating damaged periodontal tissue. The results show that DFSCs-sEVs promoted the proliferation, migration, and osteogenic differentiation of PDLSCs, potentially through the activation of the p38 MAPK signaling pathway. DFSCs-sEVs provide a novel strategy for periodontal regeneration.
STEM CELL RESEARCH & THERAPY
(2022)
Review
Chemistry, Multidisciplinary
Ilaria Roato, Beatrice Masante, Giovanni Putame, Diana Massai, Federico Mussano
Summary: This review provides an overview of recent advancements in periodontal tissue engineering, focusing on the use of PDLSCs and 3D scaffolds. The findings demonstrate the potential of these methods for innovative therapies in the future.
Article
Environmental Sciences
Yifen Shen, Chao Liu, Tao Yang, Ying Tang, Yihang Shen, Yongchun Gu
Summary: In this study, the effects of e-cigarette exposure on human gingival mesenchymal stem cells (GMSCs) and periodontal ligament stem cells (PDLSCs) were examined through RNA-seq analysis. The results showed that e-cigarette exposure primarily affected immunoregulation and inflammatory responses in GMSCs, and the microenvironment, differentiation, and corticosteroid response in PDLSCs. Additionally, a novel mechanism involving KDM6B-mediated H3K27me3 on enhancers for gene transcription regulation was identified.
ENVIRONMENTAL POLLUTION
(2023)
Review
Cell & Tissue Engineering
Liangrui Chen, Shasha Zhu, Shujuan Guo, Weidong Tian
Summary: Periodontitis is a common oral disease that causes damage to both hard and soft tissues of the periodontium, leading to tooth mobility and loss. Mesenchymal stem cells (MSCs) have emerged as a promising therapy for periodontitis due to their unique characteristics. Accumulating evidence suggests that the regenerative effects of MSCs are mainly mediated through paracrine signaling, including the release of extracellular vesicles (EVs) that contain bioactive substances. These MSC-EVs play a crucial role in regulating the biological functions of periodontal tissue cells, immune cells, and the local microenvironment, promoting tissue regeneration and repair. As a cell-free therapy, MSC-EVs offer advantages in terms of stability, immune rejection, and ethical concerns, making them potentially valuable for clinical applications in periodontal regeneration.
STEM CELL RESEARCH & THERAPY
(2023)
Review
Cell & Tissue Engineering
Aline Queiroz, Emmanuel Albuquerque-Souza, Leticia Miquelitto Gasparoni, Bruno Nunes de Franca, Cibele Pelissari, Marilia Trierveiler, Marinella Holzhausen
Summary: PDLSCs are considered a promising cell source for regenerating periodontium and have the potential to be used as an effective treatment for periodontitis.
WORLD JOURNAL OF STEM CELLS
(2021)
Review
Biotechnology & Applied Microbiology
Zeqing Zhao, Jin Liu, Michael D. Weir, Abraham Schneider, Tao Ma, Thomas W. Oates, Hockin H. K. Xu, Ke Zhang, Yuxing Bai
Summary: This review article presents the recent advances in PDLSC-based bone regeneration and periodontal tissue regeneration. By combining PDLSCs with various materials and scaffolds, as well as applying different osteoinductive agents and strategies, PDLSCs demonstrate the potential for bone and periodontal tissue regeneration.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Dentistry, Oral Surgery & Medicine
Sinan Mert, Hanna Malyaran, Rogerio B. Craveiro, Michael Wolf, Ali Modabber, Willi Jahnen-Dechent, Sabine Neuss
Summary: Clinical experience suggests that wounds in the maxilla heal faster and more efficiently compared with the mandible. This study aimed to investigate whether differences in the proliferation and differentiation of periodontal ligament stem cells (PDLSC) from the maxilla and mandible contribute to this discrepancy. The results showed that maxillary PDLSC exhibited higher proliferation and differentiation potential compared to mandibular PDLSC, indicating a possible cell-based explanation for the different healing efficacy in the maxilla and mandible.
JOURNAL OF PERIODONTOLOGY
(2023)
Article
Nanoscience & Nanotechnology
Yongxi Liang, Ajay Shakya, Xiaohua Liu
Summary: This study designed and fabricated a bioinspired tubular 3D matrix to guide the migration and growth of human periodontal ligament stem cells (PDLSCs) and form aligned fiber bundles. The tubular matrix controlled cell migration and alignment through inhibiting osteogenic differentiation. Mechanism study revealed involvement of the Yap1/Twist1 signaling pathway.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Dentistry, Oral Surgery & Medicine
A. Nagasaki, K. Nagasaki, E. Y. Chu, B. D. Kear, W. D. Tadesse, S. E. Ferebee, L. Li, B. L. Foster, M. J. Somerman
Summary: Genetic ablation of Ank, Enpp1, or both factors resulted in increased cementum regeneration, suggesting PPi modulation as a potential approach for periodontal regenerative therapies. Differences in cementum and alveolar bone regeneration in response to reduced PPi conditions highlight the need to consider tissue-specific responses in strategies targeting regeneration of the entire periodontal complex.
JOURNAL OF DENTAL RESEARCH
(2021)
Review
Cell & Tissue Engineering
Fariba Mohebichamkhorami, Roya Fattahi, Zahra Niknam, Morteza Aliashrafi, Sahar Khakpour Naeimi, Samira Gilanchi, Hakimeh Zali
Summary: Periodontal ligament stem cells (PDLSCs) have great potential for neural differentiation, capable of differentiating into various neural-like cells without the need for cell division. They can be induced towards neural lineage using a combination of nutritional factors and have significant implications for neural tissue repair when coupled with biomaterials. PDLSCs can be a valuable target for research and treatment of neurological disorders, such as Alzheimer's disease, multiple sclerosis, and cerebral ischemia. They also show beneficial effects on retinal ganglion cell regeneration and photoreceptor survival, and can be used for peripheral nerve repair through the expression of neural growth factors and differentiation into Schwann cells.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Dentistry, Oral Surgery & Medicine
A. Nagasaki, K. Nagasaki, B. D. Kear, W. D. Tadesse, V. Thumbigere-Math, J. L. Millan, B. L. Foster, M. J. Somerman
Summary: This study demonstrated that pharmacologic modulation of Pi/PPi metabolism can overcome periodontal breakdown and achieve regeneration through both in vivo and in vitro approaches.
JOURNAL OF DENTAL RESEARCH
(2021)
Article
Dentistry, Oral Surgery & Medicine
Tomoyuki Iwata, Noriyoshi Mizuno, Takayoshi Nagahara, Eri Kaneda-Ikeda, Mikihito Kajiya, Shinya Sasaki, Katsuhiro Takeda, Mari Kiyota, Ryoichi Yagi, Tsuyoshi Fujita, Hidemi Kurihara
Summary: This study investigated the regulatory genes induced by periodontal regenerative cytokines using MSCs, identifying miR-628-5p as a negative regulator of MSC stemness during periodontal regeneration. Periodontal regenerative cytokines act as suppressors of miR-628-5p to support periodontal regeneration, indicating the importance of selecting effective cytokines for different MSCs based on miRNA profiling in advancing regenerative therapies.
JOURNAL OF PERIODONTOLOGY
(2022)
Article
Cell & Tissue Engineering
Tomoya Ogawa, Mikihito Kajiya, Susumu Horikoshi, Hiroki Yoshii, Mai Yoshino, Souta Motoike, Shin Morimoto, Hisakatsu Sone, Tomoyuki Iwata, Kazuhisa Ouhara, Shinji Matsuda, Noriyoshi Mizuno
Summary: The study suggests that MSCs treated with C-MSCsy can retain their immunosuppressive and osteogenic properties after cryopreservation, providing a promising off-the-shelf cell preparation for bone regenerative therapy.
REGENERATIVE THERAPY
(2022)
Article
Cell & Tissue Engineering
Hisakatsu Sone, Mikihito Kajiya, Katsuhiro Takeda, Shinya Sasaki, Susumu Horikoshi, Souta Motoike, Shin Morimoto, Hiroki Yoshii, Mai Yoshino, Tomoyuki Iwata, Kazuhisa Ouhara, Shinji Matsuda, Noriyoshi Mizuno
Summary: Periodontal tissue regeneration can be achieved by transplanting clumps of mesenchymal stem cells (MSCs) and extracellular matrix (ECM) complexes (C-MSCs) without artificial scaffold. In a rat model, C-MSCs transplantation successfully induced periodontal tissue regeneration, with the donor cells directly involved in tissue formation. This study demonstrates a promising scaffold-free MSCs transplantation strategy for periodontal disease.
JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE
(2022)
Article
Multidisciplinary Sciences
Yuta Hamamoto, Kazuhisa Ouhara, Tsuyoshi Miyagawa, Tomoaki Shintani, Nao Komatsu, Mikihito Kajiya, Shinji Matsuda, Tsuyoshi Fujita, Shinya Sasaki, Tomoyuki Iwata, Haruya Ohno, Masayasu Yoneda, Noriyoshi Mizuno, Hidemi Kurihara
Summary: This study investigated the relationship between masticatory disorders and diabetic microvascular complications. The results showed that patients with type 2 diabetes who had diabetic neuropathy had significantly reduced masticatory efficiency. Effective mastication is an important factor in successful diet therapy for diabetes, and for patients with diabetic neuropathy, it may be necessary to combine individualized therapies from dentists and nutritionists, taking into consideration the level of masticatory dysfunction, to prevent the progression of diabetic complications.
Article
Cell & Tissue Engineering
Daisuke Kamiya, Nana Takenaka-Ninagawa, Souta Motoike, Mikihito Kajiya, Teppei Akaboshi, Chengzhu Zhao, Mitsuaki Shibata, Sho Senda, Yayoi Toyooka, Hidetoshi Sakurai, Hidemi Kurihara, Makoto Ikeya
Summary: In this study, human induced pluripotent stem cells (iPSCs) were successfully induced into mesenchymal stem cells (MSCs) under xeno-free conditions. The xeno-free-induced MSCs (XF-iMSCs) were found to have regenerative potential in bone and skeletal muscle regeneration. They promoted the regeneration of surrounding host cells through the secretion of soluble factors, and the secretion of peroxidasin and IGF2 also contributed to myotube differentiation.
NPJ REGENERATIVE MEDICINE
(2022)
Review
Health Care Sciences & Services
Toshinori Ando, Kento Okamoto, Tomoaki Shintani, Souichi Yanamoto, Mutsumi Miyauchi, J. Silvio Gutkind, Mikihito Kajiya
Summary: Genetic alterations and dysregulation of signaling pathways are crucial for the development of cancer. Precision medicine allows for the selection of appropriate treatments based on genetic variations. Various genetic alterations in HNSCC disrupt the Hippo pathway and lead to hyperactivation of YAP/TAZ.
JOURNAL OF PERSONALIZED MEDICINE
(2022)
Article
Dentistry, Oral Surgery & Medicine
Tomoyuki Iwata, Eri Kaneda-Ikeda, Keita Takahashi, Katsuhiro Takeda, Takayoshi Nagahara, Mikihito Kajiya, Shinya Sasaki, Shu Ishida, Minami Yoshioka, Shinji Matsuda, Kazuhisa Ouhara, Tsuyoshi Fujita, Hidemi Kurihara, Noriyoshi Mizuno
Summary: This study aimed to investigate the regulatory mechanism of bone marrow-derived mesenchymal stem cell (BM-MSC) differentiation by humoral factors derived from human periodontal ligament (HPL) cells and human gingival fibroblasts (HGFs). The results showed that histone deacetylase (HDAC) expression and histone H3 acetylation were involved in the regulation of BM-MSC differentiation. In clinical applications, regulation of HDAC expression holds potential for effective periodontal regeneration, particularly bone regeneration.
JOURNAL OF PERIODONTAL RESEARCH
(2023)
Article
Dentistry, Oral Surgery & Medicine
Tatsuya Akitomo, Yuria Asao, Yuko Iwamoto, Satoru Kusaka, Momoko Usuda, Mariko Kametani, Toshinori Ando, Shinnichi Sakamoto, Chieko Mitsuhata, Mikihito Kajiya, Katsuyuki Kozai, Ryota Nomura
Summary: A 4-year-and-11-month-old boy had three supernumerary teeth, which required surgical treatment and long-term follow-up. The first supernumerary tooth was extracted at 5 years and 5 months, followed by two more at 7 years and 7 months and 8 years and 2 months. Malocclusion may be caused by the supernumerary teeth, and surgical exposure was performed to facilitate the eruption of the permanent teeth.
Article
Dentistry, Oral Surgery & Medicine
Fumitaka Obayashi, Koichi Koizumi, Nanako Ito, Nami Obayashi, Tomoaki Shintani, Mikihito Kajiya, Souichi Yanamoto
Summary: Palmoplantar pustulosis (PPP) is a stubborn skin disease characterized by small aseptic pustules on the palms and soles, which can worsen due to metal and dental focal infections. This study reports a case of PPP exacerbation after orthognathic surgery, attributing it to metal allergy from the plates or screws used.
Article
Cell & Tissue Engineering
Shin Morimoto, Mikihito Kajiya, Hiroki Yoshii, Mai Yoshino, Susumu Horikoshi, Souta Motoike, Tomoyuki Iwata, Kazuhisa Ouhara, Toshinori Ando, Tetsuya Yoshimoto, Tomoaki Shintani, Noriyoshi Mizuno
Summary: Three-dimensional clumps of MSCs/ECM complexes (C-MSCs) can be implanted without artificial scaffolds, and their cellular properties can be regulated in vitro. The study aimed to generate cartilaginous tissue covered with a mineralized bone collar-like structure from human C-MSCs by combining chondrogenic and osteogenic induction, showing potential for bone-regenerative cell therapy.
STEM CELL REVIEWS AND REPORTS
(2023)
Article
Health Care Sciences & Services
Hiromi Nishi, Susumu Horikoshi, Kouji Ohta, Tetsumi Yoshida, Noriyasu Fukushima, Kyoko Oshita, Syuichi Munenaga, Taro Edahiro, Hiroshi Ureshino, Hideo Shigeishi, Yukio Yoshioka, Masaru Konishi, Noriaki Ide, Yuma Ogawa, Rikou Marukawa, Tomoaki Shintani, Natumi Ino, Mikihito Kajiya, Naoya Kakimoto, Hiroki Ohge, Tatsuo Ichinohe, Hiroyuki Kawaguchi
Summary: LLLT has shown significant efficacy in preventing severe oral mucositis among hematologic cancer patients in Japan, leading to a lower incidence rate. This study fills the gap in clinical adoption of LLLT in Japan.
JOURNAL OF PERSONALIZED MEDICINE
(2023)
Article
Cell & Tissue Engineering
Hiroki Yoshii, Mikihito Kajiya, Mai Yoshino, Shin Morimoto, Susumu Horikoshi, Misako Tari, Souta Motoike, Tomoyuki Iwata, Kazuhisa Ouhara, Toshinori Ando, Tetsuya Yoshimoto, Tomoaki Shintani, Noriyoshi Mizuno
Summary: This study investigates the role of mechanotransducer YAP/TAZ and their binding target transcriptional factor, TEAD, in the immunomodulatory capacities of human bone marrow-derived MSCs. The results show that disrupting the YAP/TAZ-TEAD axis can enhance the immunomodulatory properties of MSCs.
STEM CELL REVIEWS AND REPORTS
(2023)