Article
Biochemistry & Molecular Biology
Yang Zhou, Yanhua Cui, Chao Suo, Qian Wang, Xiaojun Qu
Summary: The study showed that EPS-M2 produced by Streptococcus thermophilus CS6 exhibited good rheology, thermostability, and antioxidant activity, mainly composed of galactose, arabinose, and glucose. The EPS-M2 has potential applications in the food industry as a natural thickener, stabilizer, and antioxidant agent.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Biochemistry & Molecular Biology
Wei Xia, Jin Han, Shiming Zhu, Yilin Wang, Wenqing Zhang, Zhengjun Wu
Summary: EPS53, a homogeneous exopolysaccharide (EPS), was isolated from Streptococcus thermophiles XJ53 fermented in skimmed milk and had a high molecular weight and a specific repeating structural unit. EPS53-D, obtained by deproteinization using TCA, had a lower molecular weight but the same repeating structural unit. EPS53 exhibited stronger immune activity than EPS53-D, indicating the importance of molecular weight in EPS activity. TCA treatment may affect the activities of native EPSs produced by fermentation.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Agriculture, Multidisciplinary
Ling Li, Ling Zhou, Xuemin Liu, Jinyan Gong, Gongnian Xiao
Summary: This study isolated and characterized Streptococcus thermophilus with high β-galactosidase activity and used it as a starter culture with Lactobacillus delbrueckii subsp. bulgaricus to make yogurt. The optimal fermentation ratio and temperature were found to be 2:1 and 42℃, respectively, leading to better organoleptic and physical properties of the yogurt. Higher lactobacilli population was observed in yogurt fermented at 37℃, and yogurt with a ratio of 2:1 starter cultures utilized lactose more effectively. The sensory properties of the yogurt remained unaffected at lower fermentation temperatures of 30 and 37℃.
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
(2023)
Article
Agriculture, Dairy & Animal Science
Yunchao Wa, Chenchen Zhang, Gulin Sun, Hengxian Qu, Dawei Chen, Yujun Huang, Ruixia Gu
Summary: Increasing the concentrations of histidine, isoleucine, and glutamate can significantly enhance the production of free exopolysaccharide (f-EPS) and the viability of Streptococcus thermophilus cells. This increase is achieved by up-regulating the transcription of EPS biosynthesis genes and enhancing the activity of key enzymes involved in sugar nucleotide synthesis. Furthermore, the higher concentrations of histidine, isoleucine, and glutamate result in increased lactose consumption and decreased galactose secretion, indicating the improved production of f-EPS.
JOURNAL OF DAIRY SCIENCE
(2022)
Article
Food Science & Technology
Xinyi Gu, Rongling Zhang, Jiancun Zhao, Cunjie Li, Tingting Guo, Suzhen Yang, Tingting Han, Jian Kong
Summary: This study reveals that fast-acidification promotes GABA biosynthesis in Streptococcus thermophilus, subsequently protecting the strains against acid stress. These findings provide theoretical foundations for making GABA-enriched yogurt.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2022)
Article
Food Science & Technology
Evelin Korcz, Laszlo Varga, Zoltan Kerenyi
Summary: This study established a correlation between total bacterial count (TBC) and exopolysaccharide (EPS) production in a specific Streptococcus thermophilus strain, showing that EPS biosynthesis is growth-related and can be monitored through TBC for quantification purposes.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Feiwei Cao, Mingming Liang, Jianxin Liu, Yu Liu, John A. Renye Jr, Phoebe X. Qi, Daxi Ren
Summary: A novel Streptococcus thermophilus strain, ZJUIDS-2-01, and an exopolysaccharide (EPS-3A) produced by it were identified and characterized from traditional yak yogurt. EPS-3A displayed superior emulsion stability, flocculating capacity, antioxidant activity, and potential antibacterial properties compared to commercial xanthan gum (XG), indicating its potential for food and industrial applications.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Agriculture, Multidisciplinary
Zhiqiang Xiong, Huanlan Chen, Xin Song, Yongjun Xia, Lianzhong Ai
Summary: The designed molecular marker epsA can be used to rapidly screen EPS+ S. thermophilus, demonstrating its potential application in the dairy and other food industries. The specificity of the epsA probe to EPS+ S. thermophilus was confirmed, and 23 positive strains were successfully identified using this molecular marker. This method shows promise for efficient screening of EPS-producing strains for industrial applications.
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
(2022)
Article
Agriculture, Dairy & Animal Science
Z. P. Xue, X. Cu, K. Xu, J. H. Peng, H. R. Liu, R. T. Zhao, Z. Wang, T. Wang, Z. S. Xu
Summary: The synthesis of glutathione (GSH) by Streptococcus thermophilus during co-cultivation effectively enhanced the growth and stress tolerance of Lactobacillus delbrueckii ssp. bulgaricus. This resulted in an increase in the biomass and improved quality of fermented milk compared to the mutant strain without GSH synthesis.
JOURNAL OF DAIRY SCIENCE
(2023)
Article
Microbiology
Zhengyuan Zhai, Shuxin Xie, Hongxing Zhang, Huaxi Yi, Yanling Hao
Summary: Homologous over-expression of EpsC in Streptococcus thermophilus can increase the molecular weight of EPS and improve the microrheological or physical properties of yogurt.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Agriculture, Multidisciplinary
Yang Zhou, Yanhua Cui, Xiaojun Qu
Summary: This study investigated the regulatory mechanisms of EPS biosynthesis in Streptococcus thermophilus CS6 and found that soy peptone and moderate pH conditions contribute to high EPS yield. Factors such as maltose, soy peptone, and initial pH greatly influence the EPS production in CS6. Differential gene expression related to carbohydrate metabolism plays a crucial role in regulating EPS biosynthesis.
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
(2022)
Article
Food Science & Technology
Zhiqiang Xiong, Xin Tian, Guangqiang Wang, Xin Song, Yongjun Xia, Hui Zhang, Lianzhong Ai
Summary: A high-throughput screening method was developed for the rapid selection of high EPS-producing strains, which proved to be simple and efficient for the isolation of EPS-producing S. thermophilus.
FOOD RESEARCH INTERNATIONAL
(2022)
Article
Agriculture, Dairy & Animal Science
Linghui Kong, Zhiqiang Xiong, Xin Song, Yongjun Xia, Lianzhong Ai
Summary: This study developed a CRISPRi system for gene transcriptional modulation in S. thermophilus. By modulating gene expression in the EPS synthesis module, the production of EPS was increased.
JOURNAL OF DAIRY SCIENCE
(2022)
Article
Chemistry, Multidisciplinary
Jorge Yanez-Fernandez, Mirna Griselda Herrera Ovando, Larissa Patlan Ramirez, Guadalupe Ramirez-Sotelo, Cesar A. Guarin, Diana C. Castro-Rodriguez
Summary: The study aimed to characterize the dextran of Leuconostoc mesenteroides SF3, isolated from aguamiel of Agave salmiana, and optimized fermentation conditions to achieve maximum yield of dextran. The dextran produced by L. mesenteroides SF3 showed high water absorption capacity, oil absorption capacity, and emulsion activity, indicating its potential applications in the food industry. The optimized conditions for production could be utilized for commercial production of this high-quality polysaccharide.
Article
Microbiology
Rodrigo Achigar, Martina Scarrone, Genevieve M. Rousseau, Cecile Philippe, Felipe Machado, Valentina Duvos, Maria Pia Campot, Moira B. Dion, Yuyu Shao, Maria Julia Pianzzola, Sylvain Moineau
Summary: The study demonstrates that both CRISPR1 and CRISPR3 systems in Streptococcus thermophilus exhibit ectopic spacer acquisition, providing phage resistance. This phenomenon also appears to occur naturally in some strains of Streptococcus pyogenes, suggesting it is a general feature in type II-A systems.
Review
Biochemical Research Methods
Jeffrey K. Cornuault, Gabriel Byatt, Marie-Eve Paquet, Paul De Koninck, Sylvain Moineau
Summary: The zebrafish model is gaining attention for investigating microbiota-host interactions due to its low cost, ability to assess large cohorts, and availability of optical methodologies. Recent findings have revealed the modulation of zebrafish gut microbiota by exogenous microbes, nutrition, and environmental factors. This model also shows potential for assessing the impact of gut microbiota on brain development.
CURRENT OPINION IN BIOTECHNOLOGY
(2022)
Article
Multidisciplinary Sciences
Cecile Philippe, Carlee Morency, Pier-Luc Plante, Edwige Zufferey, Rodrigo Achigar, Denise M. Tremblay, Genevieve M. Rousseau, Adeline Goulet, Sylvain Moineau
Summary: This study reveals that CRISPR-Cas systems in prokaryotic cells can block ACR-containing phages by targeting the acr gene, and this selection process leads to phage mutants that can interfere without acquiring new immunity. This provides an example of ACR specifically inhibiting spacer acquisition.
NATURE COMMUNICATIONS
(2022)
Correction
Multidisciplinary Sciences
Cecile Philippe, Carlee Morency, Pier-Luc Plante, Edwige Zufferey, Rodrigo Achigar, Denise M. Tremblay, Genevieve M. Rousseau, Adeline Goulet, Sylvain Moineau
NATURE COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
Frank Oechslin, Xiaojun Zhu, Moira B. Dion, Rong Shi, Sylvain Moineau
Summary: Endolysins, produced by bacteriophages, exhibit high diversity and play a crucial role in phage-host adaptation and evolution. Genetic exchange of endolysin genes showed minimal fitness costs when recipient and donor phages infected the same bacterial strain, but increased costs when infection occurred in different strains or species. Homologous recombination between co-infecting phages allowed for natural exchange of endolysins, while adaptive mutations enabled endolysins to adapt to new phage/host environments. These findings highlight the remarkable ability of phage lytic systems to recombine and adapt, explaining their diversity and mosaicism. This knowledge has implications for the engineering of antimicrobial agents.
Article
Ecology
Martin Guillemet, Helene Chabas, Antoine Nicot, Francois Gatchich, Enrique Ortega-Abboud, Cornelia Buus, Lotte Hindhede, Genevieve M. Rousseau, Thomas Bataillon, Sylvain Moineau, Sylvain Gandon
Summary: This experimental study demonstrates how coevolution between bacteria and phages, along with competition among bacterial genotypes, leads to increased diversity of bacterial CRISPR immunity. The negative-frequency-dependent selection generated by coevolution plays a crucial role in maintaining host resistance diversity and driving the emergence of new resistance mutations. Furthermore, the asymmetries in competitive abilities among different host genotypes contribute to the evolution of hosts, allowing even the fittest genotypes to escape extinctions through the acquisition of new CRISPR immunity.
NATURE ECOLOGY & EVOLUTION
(2022)
Article
Microbiology
K. M. Damitha Gunathilake, Denise M. Tremblay, Pier-Luc Plante, Ellen C. Jensen, Kenneth W. Nickerson, Sylvain Moineau
Summary: Phage SN1 infects Sphaerotilus natans and Pseudomonas aeruginosa strains. Its genome is similar to that of Pseudomonas phage M6 and contains similar base-modifying genes.
MICROBIOLOGY RESOURCE ANNOUNCEMENTS
(2022)
Article
Biochemistry & Molecular Biology
Tue Kjaergaard Nielsen, Laura Milena Forero-Junco, Witold Kot, Sylvain Moineau, Lars Hestbjerg Hansen, Leise Riber
Summary: RNA and DNA modifications are common in all organisms and play various roles. Recent advancements in sequencing techniques have led to increased interest in studying nucleotide modifications. The use of third generation sequencing platforms allows direct detection of modified bases. These modifications are particularly prevalent in bacteriophage genomes, where they primarily function to protect DNA from degradation.
Article
Virology
Amos Lucky Mhone, Angela Makumi, Josiah Odaba, Linda Guantai, K. M. Damitha Gunathilake, Stephanie Loignon, Caroline Wangari Ngugi, Juliah Khayeli Akhwale, Sylvain Moineau, Nicholas Svitek
Summary: This study analyzed the stability of 10 different Salmonella-enteritidis phages isolated from Kenyan poultry farms, finding that they were relatively stable within a range of pHs and temperatures. The phages showed different survival times in simulated gastric and intestinal fluids, and different water sources had varying effects on their viability. These phages may be administered to chickens through drinking water and can survive in the gastrointestinal tract to prevent salmonellosis in poultry.
Article
Microbiology
Andre da Silva Xavier, Alessandra G. de Melo, Connor G. Hendrich, Denise M. Tremblay, Genevieve M. Rousseau, Pier-Luc Plante, Katrina T. Forest, Poliane Alfenas-Zerbini, Caitilyn Allen, Sylvain Moineau
Summary: Ralstonia solanacearum is a destructive plant pathogen that causes lethal bacterial wilt disease. Phages that kill R. solanacearum could offer effective and environmentally friendly wilt disease control, but only if the bacterium cannot easily evolve resistance. Research has shown that the resistance of Ralstonia solanacearum to phages is related to its Type II Secretion System (T2SS), and the phage also affects the pathogenicity of R. solanacearum.
Article
Ecology
Torben Solbeck Rasmussen, Anna Kirstine Koefoed, Ling Deng, Musemma K. Muhammed, Genevieve M. Rousseau, Witold Kot, Sabrina Sprotte, Horst Neve, Charles M. A. P. Franz, Axel Kornerup Hansen, Finn Kvist Vogensen, Sylvain Moineau, Dennis Sandris Nielsen
Summary: The CRISPR-Cas system of Eggerthella lenta has been shown to target and cleave foreign DNA in vitro and acquire new immunities in vivo using gnotobiotic mice. However, this system only provides partial immunity in the gut.
Article
Nanoscience & Nanotechnology
Abolfazl Alizadeh Sahraei, Barbara Mejia Bohorquez, Denise Tremblay, Sylvain Moineau, Alain Garnier, Faical Larachi, Patrick Lague
Summary: This study aimed to evaluate the interactions of peptides with quartz as potential flotation collectors, in order to find biofriendly alternatives to biohazardous chemical reagents in mining practices. The results showed that the flexibility of peptide chains directly affected their adsorption behavior. Overall, this study is of great significance for the rational design of peptide sequences for mineral processing.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Review
Microbiology
Cecile Philippe, Jeffrey K. Cornuault, Alessandra G. de Melo, Rachel Morin-Pelchat, Alice P. Jolicoeur, Sylvain Moineau
Summary: Over the past few decades, there has been a growing interest in lactic acid bacteria (LAB) due to their industrial use, health benefits, and ecological importance. However, phage infection poses a significant risk to LAB production and use. This review discusses the defense systems employed by LAB against phage infections and the strategies used by phages to overcome these defenses. It also explores the impact of phage-host interactions on their evolution and highlights the potential of novel defense systems discovered in other bacteria for phage research in LAB.
FEMS MICROBIOLOGY REVIEWS
(2023)
Article
Virology
Zoe A. Campbell, Nelly Njiru, Amos Lucky Mhone, Angela Makumi, Sylvain Moineau, Nicholas Svitek
Summary: Women and men in Kenya who keep chickens want to earn income, provide healthy food for their families, and expand their businesses. This study recommends design opportunities for a veterinary product that contains bacteriophages (phages) targeting pathogenic Salmonella strains, based on qualitative research. The findings highlight the interplay between gender and chicken production systems, and suggest that phages combined with oral Newcastle disease vaccine or used as a treatment for fowl typhoid could benefit both men and women in different production systems. The study also emphasizes the need for a phage product to have both preventive and treatment capabilities to compete with antibiotics in the Kenyan market.
Article
Engineering, Electrical & Electronic
S. Nazila Hosseini, Mohammad Makhdoumi Akram, Partha Sarati Das, Vahid Khojasteh Lazarjan, Denise M. Tremblay, Sylvain Moineau, Younes Messaddeq, Benoit Gosselin
Summary: This article introduces a new biosensor prototype that uses microscale electrodes to measure impedance, pH, and temperature changes caused by bacterial growth. The prototype utilizes multiple sensor geometries to optimize sensitivity and includes custom circuits such as an integrated CMOS lock-in amplifier for multifrequency impedance measurement. The system successfully monitors bacterial growth by measuring impedance, pH, and temperature, and demonstrates precise and accurate measurements.
IEEE SENSORS JOURNAL
(2023)
Article
Biochemistry & Molecular Biology
Liang Cui, Seetharamsing Balamkundu, Chuan-Fa Liu, Hong Ye, Jacob Hourihan, Astrid Rausch, Christopher Hauss, Emelie Nilsson, Matthias Hoetzinger, Karin Holmfeldt, Weijia Zhang, Laura Martinez-Alvarez, Xu Peng, Denise Tremblay, Sylvain Moinau, Natalie Solonenko, Matthew B. Sullivan, Yan-Jiun Lee, Andrew Mulholland, Peter R. Weigele, Valerie de Crecy-Lagard, Peter C. Dedon, Geoffrey Hutinet
Summary: Bacteriophages and bacteria constantly evolve new molecular tools in their arms race, and this study identifies various modifications of 7-deazaguanines and their related enzymes.
NUCLEIC ACIDS RESEARCH
(2023)
