Review
Nutrition & Dietetics
Bo Wang, Jia Xiang, Binsheng He, Songwen Tan, Wenhu Zhou
Summary: Natural ingredients in modern medicine and pharmaceutical projects often face challenges such as low solubility, poor chemical stability, and low bioavailability. Spray drying technology can improve the properties of natural ingredients and optimize their performance through flexible drug delivery systems. Dry powder inhalation (DPI) has gained significant attention in pharmaceutical research, and this review focuses on the synthesis and application of spray drying for natural ingredients in DPI.
FRONTIERS IN NUTRITION
(2023)
Article
Engineering, Chemical
Dlivan Fattah Aziz, Mostafa Rostamnezhad, Kimia Azad, Yehia Ismail Khalil, Alireza Vatanara
Summary: The aim of this study was to develop a respiratory drug delivery system utilizing montelukast sodium (MTK) microparticles as a target site, using the spray freeze drying (SFD) process. Various sugars and cyclodextrins (CDs) were tested as carriers in order to find compatible excipients for dry powder inhalers (DPIs). The physical characteristics of the collected powders were examined, and the aerodynamic behavior of the particles was assessed. The results showed that CDs were excellent excipients for use with MTK for respiratory drug delivery.
ADVANCED POWDER TECHNOLOGY
(2023)
Article
Pharmacology & Pharmacy
Anna Lechanteur, Erwan Plougonven, Luisa Orozco, Geoffroy Lumay, Nicolas Vandewalle, Angelique Leonard, Brigitte Evrard
Summary: In this study, engineered inhalation powders were developed using the spray-drying technique, combining multiple carbohydrates excipients with two active pharmaceutical ingredients for asthma therapy. By adjusting the excipients, the morphology of inhalable powders could be finely tuned, leading to stable inhalation powders that induced high fine particle fractions for two drugs. The stability of these powders has been evaluated for up to 2 months at room temperature.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2022)
Article
Pharmacology & Pharmacy
E. de Pablo, P. O'Connell, R. Fernandez-Garcia, S. Marchand, A. Chauzy, F. Tewes, M. A. Dea-Ayuela, D. Kumar, F. Bolas, M. P. Ballesteros, J. J. Torrado, A. M. Healy, D. R. Serrano
Summary: A carbohydrate-based AmB dry powder inhaler (DPI) formulation was developed to address the lack of effective antifungal pulmonary treatments.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2023)
Article
Pharmacology & Pharmacy
Mani Ordoubadi, Kimberly B. Shepard, Hui Wang, Zheng Wang, Amanda M. Pluntze, Joseph P. Churchman, Reinhard Vehring
Summary: Carrier-free spray-dried dispersions for pulmonary delivery often require the addition of excipients such as L-leucine to improve efficacy. However, the presence of leucine fibers during stability has been observed. In this study, the origin of these fibers was determined to be the presence of amorphous or less stable crystalline polymorphs of leucine, but they did not affect the stability or aerosol performance of the powders.
Article
Pharmacology & Pharmacy
Susan Boc, Mohammad A. M. Momin, Dale R. Farkas, Worth Longest, Michael Hindle
Summary: This study aimed to develop an efficient dry powder inhaler (DPI) capable of delivering spray-dried Survanta-EEG powders with low volumes of dispersion air. Through iterative design modifications, an optimized DPI was achieved with a powder chamber volume of 0.21 mL, straight through airflow path, three 0.60 mm air inlet holes, and a 90 mm outlet delivery tube. The optimal device demonstrated consistent aerosol performance independent of fill mass, emitting 5.3 mg of powder at a fill mass of 10 mg. With micronized albuterol sulfate, the optimal DPI emitted over 72% of the nominal fill mass in one 3 mL actuation.
Article
Pharmacology & Pharmacy
Diana A. Fernandes, Eunice Costa, Paula Leandro, M. Luisa Corvo
Summary: This study presents an integrated methodology for the rapid selection of the optimal dry powder inhaler excipient system to develop a spray dried formulation of inhalable enzymes. By screening different sugars and amino acids, excipients that stabilize the quaternary structure of enzymes were selected. After spray drying, the resulting powders exhibited good aerodynamic performance.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2022)
Article
Pharmacology & Pharmacy
Petra Party, Csilla Bartos, Arpad Farkas, Piroska Szabo-Revesz, Rita Ambrus
Summary: The study focused on developing inhalable nano-in-micro dry powder inhalers containing meloxicam (MX) and additives, targeting the respiratory zone for improved therapeutic effect. By increasing the surface area and amorphization, the dissolution and diffusion of the drug were enhanced to achieve higher drug concentration in the deeper airways, showing suitable aerodynamical properties for treating local lung diseases.
Article
Food Science & Technology
Sandra Maria Oses, Leire Cantero, Miranda Crespo, Guillermo Puertas, Lara Gonzalez-Ceballos, Saul Vallejos, Miguel Angel Fernandez-Muino, Maria Teresa Sancho
Summary: This study evaluated the effects of three dehydration methods and three carrier agents on the preparation of ling-heather honey powder. Results showed that using maltodextrin as the carrier agent, vacuum drying and freeze drying yielded the best honey powder.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2021)
Article
Engineering, Chemical
Aijun Di, Shengyu Zhang, Xuan Liu, Zhenbo Tong, Siping Sun, Zengchao Tang, Xiao Dong Chen, Winston Duo Wu
Summary: This study developed microparticles with improved solubility and antioxidant activity using resveratrol as a model drug. Chitosan played a key role in adjusting drug release behavior and enhancing mucoadhesion properties.
Article
Food Science & Technology
Sandra Maria Oses, Leire Cantero, Guillermo Puertas, Miguel Angel Fernandez-Muino, Maria Teresa Sancho
Summary: Honey powder is widely used in food, cosmetic and pharmaceutical industries. This study evaluated the effects of different drying methods and carriers on the quality of honey powder and found that the drying procedures and carriers had minimal impact on the parameters and properties of honey powder.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2022)
Article
Engineering, Chemical
Annika Rautenberg, Alf Lamprecht
Summary: In this study, spray-freeze-dried (SFD) particles were analyzed for their flowability, mechanical stability, product properties, and physicochemical properties of the spraying solution. The results showed that SFD is a suitable method to produce freeze-dried flowable products that maintain their mechanical stability. Manipulating the nozzle diameter and solid content of the solution allows for control over particle size and the production of free-flowable powder.
Article
Pharmacology & Pharmacy
Mai Babenko, Raid G. Alany, Gianpiero Calabrese, Waseem Kaialy, Amr ElShaer
Summary: This study aimed to develop carrier-free and carrier-based dry powder inhaler (DPI) formulations for pulmonary delivery of glucagon-like peptide-1 (GLP-1) in Type 2 diabetes. The addition of a glycine-mannitol carrier improved the delivered dose of GLP-1, while the carrier-free formulation had higher fine particle fraction and smaller mass median aerodynamic diameter (MMAD).
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2022)
Article
Pharmacology & Pharmacy
Hairui Zhang, Yajie Zhang, Robert O. Williams, Hugh D. C. Smyth
Summary: The study focuses on using thin-film freeze-drying (TFFD) to prepare gene editing dry powder formulations containing PEGylated chitosan/CRISPR-Cas9 nanocomplexes. Six potential DPI formulations were identified and characterized, with two formulations containing 3% mannitol found to be suitable for inhalation with desired aerodynamic performance. The flow rate and inhaler dependency of these formulations were also evaluated at different conditions, demonstrating the suitability of TFFD-processed CRISPR-Cas9 polymer nanocomplexes for dry powder inhalation.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2021)
Article
Biochemistry & Molecular Biology
Aleksandra A. Jovanovic, Steva M. Levic, Vladimir B. Pavlovic, Smilja B. Markovic, Rada V. Pjanovic, Verica B. Dordevic, Viktor Nedovic, Branko M. Bugarski
Summary: The study compared freeze drying and spray drying for processing wild thyme drugs, finding that freeze drying was more effective in terms of total powder yield and polyphenol content. Gelatin as a coating agent improved TPC recovery and reduced material deposition on the drying chamber wall, leading to differences in polyphenol content in the resulting powders.
Article
Biochemical Research Methods
Kohei Miyata, Masami Ukawa, Kohta Mohri, Kozue Fujii, Mako Yamada, Sohei Tanishita, Satoshi Higashitarumi, Seiya Ishizaki, Hironori Kumagai, Kyohei Ochiai, Ken-ichiro Hiwatari, Kazufumi Tsubaki, Koichi Shigeno, Etsuo Tobita, Hideo Kobayashi, Shinji Sakuma
BIOCONJUGATE CHEMISTRY
(2018)
Article
Pharmacology & Pharmacy
Takehiro Noda, Tomoyuki Okuda, Ryota Mizuno, Tetsuya Ozeki, Hirokazu Okamoto
BIOLOGICAL & PHARMACEUTICAL BULLETIN
(2018)
Article
Medicine, Research & Experimental
Takaaki Ito, Tomoyuki Okuda, Yoshimasa Takashima, Hirokazu Okamoto
MOLECULAR PHARMACEUTICS
(2019)
Article
Medicine, Research & Experimental
Qui Ren, Kohta Mohri, Shota Warashina, Yasuhiro Wada, Yasuyoshi Watanabe, Hidefumi Mukai
MOLECULAR PHARMACEUTICS
(2019)
Article
Pharmacology & Pharmacy
Hironori Kumagai, Kosuke Yamada, Kanako Nakai, Tokio Kitamura, Kohta Mohri, Masami Ukawa, Takumi Tomono, Takaaki Eguchi, Testuya Yoshizaki, Takumi Fukuchi, Takuya Yoshino, Minoru Matsuura, Etsuo Tobita, Wellington Pham, Hiroshi Nakase, Shinji Sakuma
EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS
(2019)
Article
Chemistry, Medicinal
Takaaki Ito, Tomoyuki Okuda, Ryo Takayama, Hirokazu Okamoto
JOURNAL OF PHARMACEUTICAL SCIENCES
(2019)
Article
Pharmacology & Pharmacy
Tomoyuki Okuda, Yoko Toyoda, Takashi Murakami, Hirokazu Okamoto
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2019)
Article
Pharmacology & Pharmacy
Takaaki Ito, Maino Fukuhara, Tomoyuki Okuda, Hirokazu Okamoto
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2020)
Review
Chemistry, Medicinal
Tomoyuki Okuda, Hirokazu Okamoto
CHEMICAL & PHARMACEUTICAL BULLETIN
(2020)
Article
Pharmacology & Pharmacy
Kohta Mohri, Emi Hayashi, Manato Nishino, Nao Matsushita, Sohei Tanishita, Makiya Nishikawa, Shinji Sakuma
Summary: In this study, Guanine-rich oligonucleotide (GRO) was developed as an effective anticancer agent with high selectivity and antiproliferative activity in cancer cells. By incorporating GRO29A into polypod-like structured DNA, the potency of the drug was increased, showing high cytotoxicity against nucleolin-positive cancer cells.
JOURNAL OF DRUG TARGETING
(2021)
Article
Biotechnology & Applied Microbiology
Andrew Tae-Jun Kwon, Kohta Mohri, Satoshi Takizawa, Takahiro Arakawa, Maiko Takahashi, Bogumil Kaczkowski, Masaaki Furuno, Harukazu Suzuki, Shunsuke Tagami, Hidefumi Mukai, Erik Arner
Summary: This study demonstrated the creation of multiple mutant variants of U87MG cells using gene editing techniques, showing that loss of p53 function led to major transcriptional differences in the cells. Transcriptome data was used to predict mutant clones with less divergent phenotypes, which served as the best candidates for drug delivery testing platforms. In vitro and in vivo experiments supported these predictions, ultimately leading to the successful selection of the best qualifying mutant clone.
JOURNAL OF BIOTECHNOLOGY
(2021)
Article
Pharmacology & Pharmacy
Kana Suenaga, Daiki Hira, Eri Ishido, Hiroyoshi Koide, Satoshi Ueshima, Tomoyuki Okuda, Masafumi Yamaguchi, Shin-ya Morita, Hirokazu Okamoto, Tomonobu Okano, Yasutaka Nakano, Tomohiro Terada, Mikio Kakumoto
Summary: The study revealed that 33.3% of participants held the inhaler at an incorrect angle during the drug-loading step. In vitro experiments showed that holding the inhaler at an incorrect angle significantly decreased OE (an index of the loaded dose) by 73.3%, while St2 (an index of the deagglomerating efficiency) remained stable regardless of the holding angle. OE x St2, indicating the bronchial and pulmonary drug delivery amount, decreased by 76.9%. Holding the inhaler at an incorrect angle reduces the loaded dose, leading to decreased pulmonary delivery.
BIOLOGICAL & PHARMACEUTICAL BULLETIN
(2021)
Article
Multidisciplinary Sciences
Misa Ichikawa, Naomi Muramatsu, Wataru Matsunaga, Takahiro Ishikawa, Tomoyuki Okuda, Hirokazu Okamoto, Akinobu Gotoh
Summary: This study developed an orally inhalable gene therapy drug for the treatment of lung cancer. The drug effectively decreased cell proliferation and directly impacted the lungs. It provides a novel gene therapy option that patients can self-administer.
SCIENTIFIC REPORTS
(2022)
Article
Pharmacology & Pharmacy
Shota Warashina, Maki Zouda, Kohta Mohri, Yasuhiro Wada, Kazuya Maeda, Yasuyoshi Watanabe, Hidefumi Mukai
Summary: A method of labeling small extracellular vesicles (sEVs) with Cu-64 was developed and applied to pharmacokinetics study. The labeled sEVs rapidly disappeared from the systemic blood circulation and mainly accumulated in the liver and spleen, suggesting the involvement of macrophages.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2022)
Article
Pharmacology & Pharmacy
Kohta Mohri, Kim Phuong Huynh Nhat, Maki Zouda, Shota Warashina, Yasuhiro Wada, Yasuyoshi Watanabe, Shunsuke Tagami, Hidefumi Mukai
Summary: In this study, we generated a PET probe based on MccJ25 analog with an RGD motif and investigated its utility for tumor imaging. The probe showed high accumulation in tumors with integrin alpha v beta 3 expression and low non-specific accumulation in other tissues. These results suggest that MccJ25 variants can be useful tools for cancer molecular imaging.
EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES
(2023)
Review
Chemistry, Multidisciplinary
Zhiyuan Shi, Yong Hu, Xin Li
Summary: Controlled drug delivery systems that can respond to mechanical force offer a unique solution for on-demand drug activation and release. Among various mechanical stimuli, ultrasound (US) has advantages in achieving spatiotemporally controlled drug release. Traditional US-triggered drug release relies on heat-induced phase transitions or chemical transformations, while the cutting-edge approach of Sonopharmacology leverages polymer mechanochemistry. The remaining challenges and potential future directions in this field are also discussed.
JOURNAL OF CONTROLLED RELEASE
(2024)
Article
Chemistry, Multidisciplinary
Lijie Zheng, Yuanzheng Chen, Xun Gu, Yingying Li, Hanqing Zhao, Wenjun Shao, Tao Ma, Chuanbin Wu, Qingqing Wang
Summary: In this study, a novel dosage form consisting of dissolving microneedles and an adhesive transdermal patch was developed for the treatment of rheumatoid arthritis. In vitro and in vivo experiments demonstrated that the combination of drugs delivered by this dosage form effectively reduced joint inflammation and damage.
JOURNAL OF CONTROLLED RELEASE
(2024)
Article
Chemistry, Multidisciplinary
Kyungjik Yang, Hwa Seung Han, Seung Hwan An, Kyung Hoon Park, Keonwook Nam, Shinha Hwang, Yuyeon Lee, Sung Yeon Cho, Taehyung Kim, Deokyeong Choe, Sang Won Kim, Wonkyu Yu, Hyunah Lee, Jiyong Park, Sangguan You, Dong- Gyu Jo, Ki Young Choi, Young Hoon Roh, Jae Hyung Park
Summary: This study developed CP-loaded CS microcapsules to enhance the oral bioavailability of CP through controlled gastrointestinal delivery. The optimized microcapsules exhibited desirable physicochemical properties, showed anti-photoaging effects via antioxidant activity, and achieved controlled release in the gastrointestinal tract. This research provides a simple and economical approach for enhancing the oral bioavailability of CP for customized bioactive compound administration.
JOURNAL OF CONTROLLED RELEASE
(2024)
Article
Chemistry, Multidisciplinary
Huiyang Li, Shuo Liu, Wenjin Dai, Bingmei Yao, Yong Zhou, Sujia Si, Hairong Yu, Riguang Zhao, Fang Jin, Liqun Jiang
Summary: Changes in bodily fluid pressures are crucial in diseases like high-altitude pulmonary edema (HAPE). Researchers have developed hydrostatic pressure-sensitive multivesicular liposomes (PSMVLs) that can release drugs in response to pressure changes, with potential applications in HAPE treatment. Animal experiments showed that this system provides better protection for lung tissues and respiratory function, reducing the occurrence of pulmonary edema.
JOURNAL OF CONTROLLED RELEASE
(2024)
Article
Chemistry, Multidisciplinary
Qian Hu, Hongbing Lan, Yinmei Tian, Xiaonan Li, Mengmeng Wang, Jiao Zhang, Yulin Yu, Wei Chen, Li Kong, Yuanyuan Guo, Zhiping Zhang
Summary: Coacervate droplets formed through liquid-liquid phase separation have potential as delivery vesicles for therapeutics. However, their lack of physiological stability and membranes are challenges. In this study, polylysine-polynucleotide complex coacervate droplets with favorable stability were formulated to concentrate molecules and nanoparticles. Phospholipid membranes were further coated on the droplets to create coacervate-based artificial protocells (ArtPC) with membrane-like structures. These biofunctional ArtPC effectively reduced blood uric acid levels and prevented renal injuries.
JOURNAL OF CONTROLLED RELEASE
(2024)
Article
Chemistry, Multidisciplinary
Xiaowei Wang, Hongwei Lu, Fang Luo, Dan Wang, Apeng Wang, Xuelei Wang, Wenkai Feng, Xiaobo Wang, Jiayi Su, Mingliang Liu, Guimin Xia
Summary: Four novel lipid-like GEM diesters were synthesized and encapsulated into liposomes to improve the antitumor efficacy of Gemcitabine. The liposomes loaded with dimyristoyl GEM (LipodmGEM) showed enhanced cellular uptake, improved inhibition of cell migration, and a greatly extended half-life compared to free Gemcitabine. LipodmGEM successfully enriched the drug in the tumor and exhibited excellent anticancer efficacy in vivo with negligible systemic toxicity.
JOURNAL OF CONTROLLED RELEASE
(2024)
Article
Chemistry, Multidisciplinary
Pengyu Li, Jieyi Pan, Yating Dong, Yingying Sun, Yalong Wang, Kang Liao, Yili Chen, Xin Deng, Shihui Yu, Haiyan Hu
Summary: Chronic pulmonary infection caused by Pseudomonas aeruginosa is a serious public health problem with high mortality rates. In this study, infection-microenvironment responsive nanoparticles were developed to eradicate biofilms and inhibit virulence. These nanoparticles showed promising results in treating chronic pulmonary infections.
JOURNAL OF CONTROLLED RELEASE
(2024)
Article
Chemistry, Multidisciplinary
Yajing Sun, Ze Lu, John A. Taylor, Jessie L. S. Au
Summary: A recent development in cancer chemotherapy is the use of cytotoxics to induce tumor-specific immune response through immunogenic cell death (ICD). This study describes a method that utilizes immunostaining and machine-learning to identify cells with ecto-CRT in intact 3-dimensional tissues. The method was successfully applied to study drug-induced ICD in human bladder cancer.
JOURNAL OF CONTROLLED RELEASE
(2024)
Review
Chemistry, Multidisciplinary
Rafat Ali, Shantanu Sen, Rohil Hameed, Aamir Nazir, Sandeep Verma
Summary: This review provides a focused overview of emerging strategies for delivering gasotransmitters in a controlled and sustained manner to reestablish neurophysiological homeostasis.
JOURNAL OF CONTROLLED RELEASE
(2024)
Article
Chemistry, Multidisciplinary
Jing Chen, Xinyi Zhang, Jinshen Zhang, Zhaoxia Wang, Guilan Zhu, Ming Geng, Jinmiao Zhu, Yajun Chen, Wei Wang, Youcui Xu
Summary: In this study, a multifunctional responsive hydrogel system was developed for synergistic reoxygenation and chemo/photothermal therapy. The hydrogel system showed both therapeutic effects against metastatic breast cancer and wound infection, making it a promising strategy for treating and preventing tumor recurrence and associated wound infection.
JOURNAL OF CONTROLLED RELEASE
(2024)
Article
Chemistry, Multidisciplinary
Baoshan Huang, Na Zhang, Xinying Qiu, Rui Zeng, Shuimiao Wang, Mengxia Hua, Qing Li, Kaihui Nan, Sen Lin
Summary: This study revealed that robust ROS can oxidize mitochondrial DNA (ox-mtDNA) and cause its release into the cytosol, resulting in the activation of NLRP3 inflammasome. By using the mitochondria-targeted antioxidant SkQ1 and a novel mitochondria-targeted nanoparticle (SkQ1 NP), it was found that mitochondrial ROS scavenging could in situ inhibit DED-induced mtDNA oxidation and suppress NLRP3-mediated inflammation.
JOURNAL OF CONTROLLED RELEASE
(2024)
Article
Chemistry, Multidisciplinary
Wenqi Liu, Cheng Hu, Linyu Long, Shuyi He, Wen Zhang, Zhicun Wang, Li Yang, Yunbing Wang
Summary: Myocardial infarction is the leading cause of cardiovascular mortality, and current treatment methods have limitations. This study developed a smart carrier that can release different therapeutic substances for different pathological processes, effectively improving cardiac function, promoting cardiac repair, and preventing ventricular remodeling.
JOURNAL OF CONTROLLED RELEASE
(2024)