Article
Polymer Science
M. Bhagya Dharmasiri, T. Kuruwita Mudiyanselage
Summary: Highly porous and fast-responding PNIPAM hydrogels were prepared by emulsion-templated polymerization using virgin coconut oil, showing approximately seven times faster response rate than conventional PNIPAM hydrogels.
Article
Polymer Science
Fang Yin, Pascale Laborie, Barbara Lonetti, Yannick Coppel, Nancy Lauth-de Viguerie, Jean-Daniel Marty, Stephane Gineste
Summary: Doubly thermo-and pH-responsive PNIPAM-b-PDEAEAM polymers were synthesized by RAFT polymerization. The properties of these polymers and corresponding homopolymers in solution depend on pH and the protonation degree of the diethylamino moiety. The behavior of the polymers is also influenced by the nature of the polymer chain ends. At pH 10, both PNIPAM and PDEAEAM blocks contribute to phase transition and form stable spherical nanoobjects. At pH 4, PNIPAM-b-PDEAEAM with longer PDEAEAM length exhibits a two-step thermoresponsive behavior and forms large aggregates with a positively charged shell.
Article
Polymer Science
Jun He, Dongni Lin, Ying Chen, Li Zhang, Jianbo Tan
Summary: A new method has been developed in this study to prepare PNIPAM-based block copolymer nanoparticles with different morphologies through aqueous photoinitiated polymerization-induced self-assembly, and it was found that the reaction temperature plays a crucial role in both morphology and polymerization kinetics of the nanoparticles.
MACROMOLECULAR RAPID COMMUNICATIONS
(2021)
Article
Materials Science, Paper & Wood
Muhammad Asif Iqbal, Toheed Akhter, Muhammad Faheem, Asif Mahmood, Waheed Al-Masry, Sohail Nadeem, Sadaf Ul Hassan, Chan Ho Park
Summary: We synthesized a thermoresponsive polymer with a narrow molecular weight distribution using organic photo-redox-catalyzed atom transfer radical polymerization (O-ATRP). The O-ATRP was mediated by a photoredox catalyst 1,2,3,5-tetrakis (carbazol-9-yl)-4,6-dicyanobenzene (4CzIPN) under visible light irradiation. The polymerization of polymethyl methacrylate (PMMA) chains from a hydroxypropyl cellulose (HPC)-based macroinitiator was characterized using various analytical methods. The resulting PMMA-grafted HPC (PMMA-g-HPC) showed a widened lower critical solution temperature (LCST) window and an inverse pH dependency of the LCST, making it promising for various applications including drug delivery systems.
Article
Engineering, Chemical
Hui Ye, Yining Zhou, Guodong Yang, Tengfei Yu, Yuzhong Zhang, Lizhi Zhao, Qingping Xin, Shurui Han
Summary: In this study, a pH-responsive membrane was developed for protein fractionation. The membrane successfully separated different milk proteins by controlling the pH value.
POLYMER ENGINEERING AND SCIENCE
(2022)
Article
Polymer Science
Isaac J. Gresham, Ben A. Humphreys, Joshua D. Willott, Edwin C. Johnson, Timothy J. Murdoch, Grant B. Webber, Erica J. Wanless, Andrew R. J. Nelson, Stuart W. Prescott
Summary: The structure of grafted-from poly(N-isopropylacrylamide) brushes under confinement shows a uniform internal structure and specific volume fraction distribution, eliminating the critical transition point in their thermal response. Different experimental methods find qualitative agreement, but variations exist due to path-dependent behavior.
Article
Engineering, Chemical
Sumito Ishii, Akinori Sezai, Yasuhiro Kohsaka, Shigeru Deguchi, Mitsumasa Osada
Summary: This study achieved high-speed radical polymerization of N,N-diethyl acrylamide in high-temperature water without using organic solvents. The resulting polymer had comparable molecular weight to that obtained by typical polymerization, but with lower environmental impact.
INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
(2022)
Article
Polymer Science
Gianluca Ciarleglio, Elisa Toto, Maria Gabriella Santonicola
Summary: In this study, conductive composite hydrogels were synthesized by embedding carboxyl-functionalized multi-walled carbon nanotubes (MWCNT-COOH) in a poly(N-isopropylacrylamide) (PNIPAM) matrix through a two-step photopolymerization method. The concentration of the carbon nanotubes was varied to obtain thermo-responsive hydrogels with controlled hydrophilicity and conductivity. The results showed that increasing the nanotube concentration shifted the endothermic peak associated with the temperature-induced volume phase transition (VPT) to higher temperatures, indicating a stronger hydrogen bond dissociation. In PBS solution, the swelling ratios and VPT temperatures were reduced, and the electrical resistivity decreased by a factor of 10 compared to water-swollen hydrogels.
Article
Chemistry, Analytical
Yaping Li, Ningning Sun, Songxin Ma, Xin Zhang, Yingfeng Wang, Xingru Li
Summary: A magnetic thermo-responsive branched polymer was synthesized and applied for the extraction and analysis of phenolic acids in olive oil samples. The extraction performance was enhanced by controlling the molecular weight and thermo-responsive characteristics. The microwave-assisted method achieved a rapid sample pretreatment process.
ANALYTICA CHIMICA ACTA
(2022)
Article
Nanoscience & Nanotechnology
Kai Wang, Guoqi Chen, Sen Weng, Linxi Hou, Dezhan Ye, Xiancai Jiang
Summary: A thermo-responsive PNIPAM/HPMC hydrogel was prepared using a simple one-step low-temperature polymerization method, achieving high transmittance, high solar modulation, low phase-transition temperature, and excellent high-temperature thermal stability simultaneously. The hydrogel exhibited high light transmittance (90.82%) and good mechanical properties and thermal stability. It also showed a low phase-transition temperature (about 32 degrees C) and high solar modulation (81.52%), making it an ideal material for thermochromic smart windows.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Chemistry, Multidisciplinary
Yulia Eygeris, Noah Ulery, Ilya Zharov
Summary: Novel pH-responsive nanoporous membranes were prepared by self-assembly, showing controllable permeability and pore size. Water flux measurements revealed that the pore size of the membranes was mostly determined by the swelling of PDMAEMA brushes and their permeability to water, while hexane flux measurements were more strongly influenced by the length of the polymer brush. The flux and pore size of the membranes also exhibited significant changes under different pH conditions.
Article
Chemistry, Analytical
Kikuo Komori, Ryo Ihara, Seiryu Hirao, Minghao Liu, Yuki Toyota, Mitsutoshi Nakata, Yuta Tani, Kohei Shiraishi
Summary: The reversible activation and inhibition of electrochemical reactions can be achieved by synthesizing a thermo-responsive polymer on the surface of a cup-stacked carbon nanofiber-modified electrode. The phase transition of the polymer can be controlled by adjusting the temperature and using near-infrared light irradiation, enabling the tunability of the electron transfer reaction of ions.
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
(2022)
Article
Polymer Science
Ryo Satoh, Saika Honma, Hiroyuki Arafune, Ryo Shomura, Toshio Kamijo, Takashi Morinaga, Takaya Sato
Summary: This study developed a novel method of in situ surface-initiated atom-transfer radical polymerization using nonvolatile ionic liquid components to form concentrated polymer brushes on a silicon wafer, which was confirmed by gel permeation chromatography. The effectiveness of in situ preparation for CPBs using the coating technique was established for the first time in this paper.
Article
Polymer Science
Aggeliki Stamou, Hermis Iatrou, Constantinos Tsitsilianis
Summary: This study developed PLL-g-NIPAm with LCST thermosensitivity by grafting N-isopropylacrylamide (NIPAm) onto polylysine (PLL), and the LCST is influenced by pH and ionic strength.
Article
Chemistry, Analytical
Hiroki Toda, Wataru Iwasaki, Nobutomo Morita, Taisei Motomura, Kenshin Takemura, Masaya Nagano, Yoshitaka Nakanishi, Yuta Nakashima
Summary: A thermo-responsive valve was fabricated by plasma-induced graft polymerization, allowing for control of opening and closing based on temperature. Results suggest that this valve can effectively open and close without subsequent loss of performance.
Article
Multidisciplinary Sciences
Kenichi Nagase, So Inoue, Masakazu Inoue, Hideko Kanazawa
Summary: In this study, a new two-dimensional chromatography system using PNIPAAm-modified silica beads was developed for effective and safe drug monitoring. The system separates serum proteins and drugs by adjusting the temperature and uses all-aqueous mobile phases.
SCIENTIFIC REPORTS
(2022)
Article
Engineering, Chemical
Kenichi Nagase, Sakiko Kitazawa, Toshihiko Kogure, Sota Yamada, Kazuhiro Katayama, Hideko Kanazawa
Summary: A temperature-modulated viral vector purification column using a mixed polymer brush as packing material was developed in this study. The column successfully eluted the AAV2 vector and separated it from bovine serum proteins.
SEPARATION AND PURIFICATION TECHNOLOGY
(2022)
Article
Multidisciplinary Sciences
Kenichi Nagase, Kosuke Matsumoto, Hideko Kanazawa
Summary: In this study, mixed-mode chromatography columns were developed using PNIPAAm and PAPTAC brush-modified silica beads and evaluated on various analytes. The results showed that the columns could effectively separate different compounds through modulating hydrophobic and electrostatic interactions.
SCIENTIFIC REPORTS
(2022)
Article
Multidisciplinary Sciences
Kenichi Nagase, Hikaru Takagi, Hideo Nakada, Haruki Ishikawa, Yoshiko Nagata, Tohru Aomori, Hideko Kanazawa
Summary: Therapeutic drug monitoring is critical in pharmacological therapy. In this study, thermoresponsive chromatography columns with various cationic properties were developed for effective drug monitoring.
SCIENTIFIC REPORTS
(2022)
Article
Biochemistry & Molecular Biology
Kento Takaya, Ayano Sunohara, Noriko Aramaki-Hattori, Shigeki Sakai, Keisuke Okabe, Hideko Kanazawa, Toru Asou, Kazuo Kishi
Summary: This study analyzed the expression and localization of Wnt proteins in a mouse fetal-wound-healing model and found their association with scar formation. The results indicated that Wnt signaling may be involved in wound healing and scar formation. Regulation of Wnt signaling could be a possible mechanism to control scar formation.
Article
Biophysics
Kenichi Nagase, Akari Okada, Junnosuke Matsuda, Daiju Ichikawa, Yutaka Hattori, Hideko Kanazawa
Summary: In this study, a thermoresponsive cationic block copolymer brush-grafted beads were developed as packing materials for ADSC separation columns. These columns can adsorb ADSCs at 37 degrees C and elute them at 4 degrees C, while other cells are not adsorbed. This separation column can be used to isolate ADSCs without cell surface modification, while maintaining cell activity.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2022)
Article
Chemistry, Physical
Hironobu Takahashi, Haruno Wakayama, Kenichi Nagase, Tatsuya Shimizu
Summary: This study presents a multilayering approach to produce engineered human muscle tissue with sufficient contractile force. The tissue shows unidirectional contraction and can quantitatively measure drug effects.
Article
Engineering, Biomedical
Kenichi Nagase, Kaichi Yamazaki, Yutaro Maekawa, Hideko Kanazawa
Summary: Temperature-modulated exosome-capturing materials were developed using thermoresponsive polymers and peptides with affinity for exosomes. The copolymer-grafted silica beads successfully captured and released targeted exosomes via temperature modulation.
MATERIALS TODAY BIO
(2023)
Article
Engineering, Biomedical
Kenichi Nagase, Haruno Wakayama, Junnosuke Matsuda, Naoto Kojima, Hideko Kanazawa
Summary: Thermoresponsive mixed polymer brushes were developed to modulate cell adhesion through temperature change. These brushes enhanced cell adhesion and exhibited thermoresponsive adhesion and detachment properties with different cell types.
MATERIALS TODAY BIO
(2023)
Article
Cell & Tissue Engineering
Jun Kobayashi, Teruo Okano
Summary: Hepatocyte transplantation has been used for the treatment of congenital metabolic liver diseases and liver failure support. Hepatocyte sheet technology using PIPAAm-grafted dishes provides an efficient method for cell transplantation. Shortening the medium depth can maintain hepatocyte monolayer and improve oxygen supply to the cells. The hepatocyte sheets can be easily detached from the dishes and transferred to target sites.
TISSUE ENGINEERING PART C-METHODS
(2023)
Article
Chemistry, Physical
Kenichi Nagase, Junnosuke Matsuda, Ayako Takeuchi, Yuka Ikemoto
Summary: The hydration and dehydration behaviors of PNIPAAm-grafted silica beads were analyzed using FTIR spectroscopy. The results showed that the peak intensity and position of the FTIR spectra varied with different relative humidity and temperature conditions, indicating the strong hydration and dehydration properties of PNIPAAm.
SURFACES AND INTERFACES
(2023)
Article
Materials Science, Biomaterials
Kenichi Nagase, Naoto Kojima, Mitsuaki Goto, Toshihiro Akaike, Hideko Kanazawa
Summary: In this study, a hepatocyte cell separation material was developed using a thermoresponsive polymer and a polymer with high affinity to hepatocytes. The temperature modulation allowed for the effective separation of hepatocytes from other cells. This material holds significance for cell therapy and liver tissue engineering.
JOURNAL OF MATERIALS CHEMISTRY B
(2022)
Correction
Biochemical Research Methods
Yong Wang, Suhua Yu, Rui Li, Jing Wan, Yuheng Wang, Zongli Huo, Chunyong Wu, Li Mi, Songqin Liu
JOURNAL OF CHROMATOGRAPHY A
(2024)
Review
Biochemical Research Methods
Israel D. Souza, Maria Eugenia C. Queiroz
Summary: Organic-silica hybrid monolithic materials have gained significant attention in separation science and sample preparation due to their unique properties. This review summarizes the characteristics, current status, and recent developments of these materials, as well as discusses synthesis methods, characterization techniques, and strategies for improving extraction efficiency in various applications.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Bhagwat Upreti, Bhanu Prasad Vempatapu, Jagdish Kumar, Pankaj K. Kanaujia
Summary: Lubricating oils are essential for the effective performance of internal combustion engines. This study presents a new HPLC method for determining additives in finished lubricating oils and polar substances in used engine oils.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zongqing Huang, Haoju Hua, Xiuzhen Du, Zipeng Zhen, Wei Zhao, Jun Feng, Ji-an Li
Summary: This study successfully isolated VHHs against the SUMO protein for the first time using biopanning of an immune camelid nanobody library. The VHHs were coupled to agarose resins to form affinity chromatography resins, allowing for one-step purification of various SUMO fusions.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Xueting Qiu, Xuben Hou, Yue Yang, Hao Fang, Fei Cui, Xinying Yang
Summary: In this study, a new method based on electrophoretically mediated microanalysis was developed for the investigation of PTPRO enzymatic activity. The method was successfully used to determine the kinetics of PTPRO and the inhibitory concentration of PTPRO inhibitors. The method exhibited improved accuracy and reduced consumption compared to traditional methods.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Feifei Luo, Mengmeng Hao, Lei Zhang, Yangguo Xie, Wei Hou, Hongya Wang, Zhongli Zhang
Summary: In this study, a comprehensive screening and structure elucidation of 23 nonvolatile organic compounds (NVOCs) in two antibody drugs was conducted. Seven previously unreported compounds were identified. A quantitative method with high sensitivity, accuracy, and precision was developed for evaluating the safety of NVOCs in drug products.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zuzana Vosahlova, Kveta Kalfkova, Martin Gilar, Jakub Szymarek, Maria Mazurkiewicz-Beldzinska, Sylwia Studzi
Summary: A hydrophilic interaction liquid chromatography method was developed for the separation and identification of oligonucleotide impurities and metabolites, and successfully applied to the analysis of serum samples from Spinraza-treated patients.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Aswanidevi Kongasseri, Thirumalai Madhesan, Sangeetha Krishna Kumar, Sushmitha Pedugu Sivaraman, Suchashrita Mitra, Pitchaiah Kancharlapalli Chinaraga, C. V. S. Brahmmananda Rao, Sivaraman Nagarajan, Prabhakaran Deivasigamani, Akhila Maheswari Mohan
Summary: This study focuses on the sequential separation of trivalent lanthanides using modified C-18 silica-packed supports and reversed-phase HPLC technique. By tuning the column modifiers and separation parameters, the researchers achieved systematic separation of Ln(3+). The results showed that the choice of column modifiers and fine-tuning of the modification procedure were crucial for achieving excellent separation.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Raquel Perez-Robles, Szabolcs Fekete, Robert Kormany, Natalia Navas, Davy Guillarme
Summary: There is a mismatch between the sample diluent and the mobile phase in HILIC analysis, leading to analyte breakthrough and peak distortion. Adding a weak solvent for pre-injection can effectively prevent these issues and improve sensitivity.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Oleg Pokrovskiy, Irina Rostovschikova, Konstantin Ustinovich, Ilya Voronov, Dmitry Kosyakov
Summary: A chromatography-like propagation of water through the material bed was observed during supercritical fluid extraction of aroma plants with high moisture content, which could be significant for modeling the kinetics of supercritical fluid extraction of non-volatile compounds.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Yasar Kemal Recepoglu, Ozguer Arar, Asli Yuksel
Summary: Biosorption using phosphorylated hazelnut shell waste (FHS) is a promising method for lithium removal and recovery. This study investigated the performance of a continuous-flow packed-bed column system using FHS for ultra-low level lithium removal. The results showed that the lihium biosorption capacity of the FHS column was unaffected by the bed height, but decreased with increasing flow rate.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Jian Sun, Zheheng Zheng, Zixiao Jia, Jiabin Wang, Xucong Lin
Summary: In this study, an ionic covalent organic framework (iCOF) surface grafting monolithic sorbent was prepared for in-tube solid-phase microextraction (SPME) of aristolochic acids (AAs) in various samples. The density of quaternary ammonium ions in the iCOF was modulated for enhanced adsorption of AAs. The iCOF surface grafting monolithic sorbent was confirmed by different means. Several operating conditions were optimized to develop the online in-tube SPME-HPLC method, which showed good linearity, low limits of detection, and high recoveries for AAs in serum samples. This study presents a feasible approach for the application of COF-based monolithic sorbents in sample preparation.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zeng Wen, Ruihan Peng, Die Gao, Jing Lin, Jia Zeng, Zhou Li, Famin Ke, Zhining Xia, Dandan Wang
Summary: A chitosan-alginate sponge with multiple cross-linking networks was developed for adsorption and enrichment of anionic dyes from food samples. The sponge showed better three-dimensional network structure, mechanical behavior, and stability compared to pure chitosan and alginate sponges. The adsorption performance and selectivity of the sponge were systematically studied, and it maintained high adsorption capacity and selectivity for anionic dyes. The sponge also showed satisfactory reusability and was successfully combined with high-performance liquid chromatography for the enrichment and detection of anionic dyes in candy and cocktail samples.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Hairong Zhang, Xiaojuan Jiang, Dandan Zhang, Yuexin Yang, Qiang Xie, Caisheng Wu
Summary: Analysis of in vivo exposure to traditional Chinese medicine using mass spectrometry is helpful for identifying effective ingredients and developing new drugs. This study focused on Forsythia suspensa (Lian Qiao) and used metabolomics technology to explore its in vivo exposure. By comparing the differences before and after administration, a complete set of nontargeted analysis strategies for TCM exposure was established. The study identified 393 Lian Qiao-related chemical components in mice after administration, including prototypes and metabolites. This study provides a reference for research on the active ingredients of Lian Qiao in vivo.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Oscar Castejon-Musulen, Ricardo Lopez, Ignacio Ontan, Vicente Ferreira
Summary: A quantitative analytical method was developed and validated in this study to determine the concentrations of 81 aroma-relevant wine volatiles. The method utilizes stir bar sorptive extraction and multidimensional gas chromatography with flame ionization detector or mass spectrometer. The method demonstrated good linearity, reproducibility, and recovery, and was successfully applied to analyze 20 wine samples.
JOURNAL OF CHROMATOGRAPHY A
(2024)
