Article
Biotechnology & Applied Microbiology
Chaitanya Kumar Reddy Pocha, Wen Yi Chia, Kit Wayne Chew, Heli Siti Halimatul Munawaroh, Pau Loke Show
Summary: This paper discusses the extraction of fucoxanthin, focusing on Phaeodactylum tricornutum and briefly mentioning Chaetoceros calcitrans and Isochrysis galbana. The cultivation, harvesting, and drying processes of these diatoms are briefly discussed, followed by an introduction to traditional and new extraction methods. The limitations of current extraction strategies and the potential for cost-effective technologies are also discussed.
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
(2022)
Article
Multidisciplinary Sciences
A-Hyeon Lee, Hye-Yoon Shin, Jong-Hwi Park, Song Yi Koo, Sang Min Kim, Seung-Hoon Yang
Summary: Pro-inflammatory cytokines are produced and secreted through the activation of NF-kappa B and NLRP3 inflammasome signaling pathways. Fucoxanthin, found in Phaeodactylum tricornutum extracts, has been shown to inhibit the activation of these pathways and decrease the production of major pro-inflammatory cytokines.
SCIENTIFIC REPORTS
(2021)
Article
Biotechnology & Applied Microbiology
J. Camacho-Rodriguez, J. J. Gallardo-Rodriguez, M. C. Ceron-Garcia, F. Garcia-Camacho, E. Molina-Grima
Summary: The nutrient content of a commercial seawater culture medium for growing Isochrysis galbana was optimized using a genetic algorithm-based stochastic strategy, which significantly improved polyunsaturated fatty acids (PUFAs) productivity by 15% and increased carotenoids by 50% dry weight. The genetic algorithm approach also allowed for the reduction of nutrient requirements, such as N (25%), Mo (20%), Mn (60%), Co (60%), and Cu (60%).
JOURNAL OF APPLIED PHYCOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Xinjie Zhao, Liwei Gao, Xiangzhong Zhao
Summary: In this study, a two-step method was used to purify Fucoxanthin from Phaeodactylum tricornutum, resulting in a purity of 92.03% and a recovery rate of 76.67%. The identification and quantification of Fucoxanthin were determined by HPLC and ESI-MS. The purified Fucoxanthin exhibited strong antioxidant properties in vitro.
Article
Chemistry, Applied
Song Yi Koo, Keum Taek Hwang, Soonjae Hwang, Ki Young Choi, Yun Ji Park, Jae-Hyeong Choi, To Quyen Truong, Sang Min Kim
Summary: Nanoencapsulation significantly improves the bioavailability of fucoxanthin in microalgae. Both types of nanoparticles show controlled release of fucoxanthin and enhance its permeability and absorption in cells and plasma.
Article
Agricultural Engineering
Zhi-Peng Wang, Pei-Kang Wang, Yan Ma, Jia-Xue Lin, Cheng-Long Wang, Yu-Xiang Zhao, Xin-Yue Zhang, Bei-Chen Huang, Shou-Geng Zhao, Lei Gao, Jing Jiang, Hai-Ying Wang, Wei Chen
Summary: The study demonstrated that the addition of Laminaria japonica hydrolysate (LPH) is a feasible and efficient strategy to promote fucoxanthin accumulation. In the LPH group, both fucoxanthin content and biomass were significantly increased.
BIORESOURCE TECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Ting-Bin Hao, Yang Lu, Zhong-Hong Zhang, Si-Fen Liu, Xiang Wang, Wei-Dong Yang, Srinivasan Balamurugan, Hong-Ye Li
Summary: This study successfully improved carotenoid production in diatom Phaeodactylum tricornutum through genetic modification, particularly by overexpressing key carotenogenic genes involved in the MEP pathway. The elevated expression of carotenogenic genes led to increased total carotenoid content, with significant enhancement in fucoxanthin content in the engineered lines CMK and CMS.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Genetics & Heredity
Duo Chen, Xue Yuan, Xuehai Zheng, Jingping Fang, Gang Lin, Rongmao Li, Jiannan Chen, Wenjin He, Zhen Huang, Wenfang Fan, Limin Liang, Chentao Lin, Jinmao Zhu, Youqiang Chen, Ting Xue
Summary: This study presents a comprehensive analysis of the regulatory networks for fucoxanthin (Fx) biosynthesis in Isochrysis galbana, a potential bait for functional foods and nutraceuticals. A high-quality genome assembly of I. galbana LG007 was obtained, which revealed its phylogenetic relationship with other Haptophytes. Evolutionary analysis provided insights into the divergence time between I. galbana and Emiliania huxleyi. Gene family and metabolome analyses identified key genes and environmental factors influencing Fx content in I. galbana. These findings contribute to the understanding of Fx biosynthesis and regulation, providing valuable references for food and pharmaceutical applications.
GENOMICS PROTEOMICS & BIOINFORMATICS
(2022)
Article
Biotechnology & Applied Microbiology
Hugo Pereira, Marta Sa, Ines Maia, Alexandre Rodrigues, Iago Teles, Rene H. Wijffels, Joao Navalho, Maria Barbosa
Summary: This study produced two marine microalgae to evaluate biomass and fucoxanthin production, demonstrating the potential for fucoxanthin production from microalgae.
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
(2021)
Article
Biotechnology & Applied Microbiology
Xiaotong Shen, Kehou Pan, Lin Zhang, Baohua Zhu, Yun Li, Jichang Han
Summary: This study investigated the function of a protein called PtABA4 in the synthesis of fucoxanthin, a valuable carotenoid. The overexpression and knockdown of PtABA4 in Phaeodactylum tricornutum showed significant changes in fucoxanthin accumulation. It is speculated that PtABA4 may form multi-enzyme complexes with other membrane associated enzymes and participate in the formation of various carotenoids.
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
(2022)
Article
Biotechnology & Applied Microbiology
Songcui Wu, Wenhui Gu, Shuao Jia, Xiujun Xie, Lijun Wang, Guangce Wang
Summary: This study aimed to develop an efficient and cost-effective method for harvesting Phaeodactylum tricornutum and reusing the culture medium for fucoxanthin production. The results showed that the FeCl3-based flocculation method was highly effective (>93%) in harvesting the microalgae. A 50/50 proportion of recycled medium and fresh medium can be used without significant negative effects on biomass and fucoxanthin yield, while reducing water consumption by approximately 43%. Simple washing with water effectively removed residual iron from the harvested biomass or pigment, resulting in a reduction of approximately 42% and 64% respectively. These findings contribute to the development of a sustainable and cost-effective method for microalgae-based fucoxanthin production.
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
(2023)
Article
Chemistry, Medicinal
Yong Fan, Xiao-Ting Ding, Li-Juan Wang, Er-Ying Jiang, Phung Nghi Van, Fu-Li Li
Summary: This study introduces a new method for screening mutants with high fucoxanthin content, successfully identifying a linear correlation between content and fluorescence intensity in the diatom model organism rich in fucoxanthin. By using flow cytometry, high-fucoxanthin-content mutants were sorted out, with a significant increase in fucoxanthin content compared to the wild type after 20 days of cultivation. This efficient, rapid, and high-throughput approach provides a valuable tool for screening fucoxanthin-overproducing mutants.
Article
Agricultural Engineering
Thomas O. Butler, Gloria Padmaperuma, Alessandro M. Lizzul, Joe McDonald, Seetharaman Vaidyanathan
Summary: The study found that using the commercial powdered media Cell-Hi JWP for the cultivation of P. tricornutum outdoors in the UK yielded good performance characteristics and cost-effectiveness. There was a strong positive correlation between fucoxanthin and chlorophyll a content, and a weak inverse correlation between EPA content and temperature. Commensal bacterial counts showed a sinusoidal growth profile with a transition in community dominance observed.
BIORESOURCE TECHNOLOGY
(2022)
Article
Microbiology
Duo Chen, Huan Li, Jing Chen, Yuying Han, Xuehai Zheng, Yixin Xiao, Xupeng Chen, Tao Chen, Jiannan Chen, Youqiang Chen, Ting Xue
Summary: This study aimed to uncover the mechanism of fucoxanthin biosynthesis in Isochrysis galbana under green light. The results revealed that differentially accessible chromatin regions (DARs)-associated genes were enriched in carotenoid biosynthesis and photosynthesis-antenna protein formation. MYB family motifs were identified as candidates controlling metabolic regulation responses to green light. Differential expression analysis and WGCNA showed that genes related to carotenoid metabolism and photosynthesis exhibited a higher expression level under green light. Integrated analysis of ATAC-seq and RNA-seq identified specific genes for green light that may play a key role in fucoxanthin biosynthesis in I. galbana through a complex regulatory network.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Biochemical Research Methods
Mohammed Rehmanji, Asha Arumugam Nesamma, Nida Jamil Khan, Tasneem Fatma, Pannaga Pavan Jutur
Summary: In this study, the productivity of the marine diatom Phaeodactylum tricornutum, rich in omega-3 polyunsaturated fatty acids and brown pigment, was enhanced through media engineering strategy. The results showed that supplementing the medium with glycerol and urea significantly increased the biomass, proteins, lipids, carbohydrates, fatty acid methyl esters, EPA, and fucoxanthin production. The improved strategy of utilizing cost-effective substrates can provide an efficient biomass valorization paradigm for the production of high-value renewables.
BIOTECHNOLOGY JOURNAL
(2022)
Article
Biotechnology & Applied Microbiology
Jose Omar Garcia Gomez, Nancy Eloisa Rodriguez Olalde, Ana Alejandra Vargas Tah, Marco Rito-Palomares, Fabricio Napoles-Rivera, Agustin Jaime Castro Montoya
Summary: The study proposes an alternative process for the dehydration of acetone by liquid-liquid extraction using an aqueous two-phase system (ATPS). Experimental results on the acetone-water-potassium carbonate ternary system show a good correlation with the ELECNRTL thermodynamic model. The Hunter-Nash method was applied to achieve a concentration greater than 95 wt% of acetone from a 10 wt% mixture at room temperature. Simulation with Aspen Plus's decanter block yielded results very close to the experimental results, indicating potential wide application of ATPSs for dehydration of bioproducts and separation of azeotropic mixtures.
JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY
(2021)
Article
Engineering, Chemical
Antonela Taddia, Marco Rito-Palomares, Karla Mayolo-Deloisa, Gisela Tubio
Summary: This study developed an integrated bioseparation strategy for the purification of fungal cellulase-free xylanase, utilizing liquid-liquid extraction and ion exchange chromatography. The extracted xylanase demonstrated high purity and recovery rate, showing potential for industrial application as a high-quality enzyme.
SEPARATION AND PURIFICATION TECHNOLOGY
(2021)
Article
Engineering, Chemical
Mario A. Torres-Acosta, Nathalia dos Santos, Sonia P. M. Ventura, Joao A. P. Coutinho, Marco Rito-Palomares, Jorge F. B. Pereira
Summary: This study evaluated the potential and commercial applicability of using ATPS for GFP purification, finding that PEG-based ATPS has lower costs but improvements in purity are desired. Through sensitivity analysis, it was discovered that increasing the production titre of the IL-based system can lower production costs compared to the traditional PEG-based system.
SEPARATION AND PURIFICATION TECHNOLOGY
(2021)
Article
Engineering, Chemical
Maximilian Nisslein, Mirna Gonzalez-Gonzalez, Marco Rito-Palomares
Summary: The study investigated the influence of parameters on the partition behavior of human peripheral blood and CD34 antibody in different ATPS systems, suggesting that volume ratio has a stronger effect on the separation behavior.
SEPARATION AND PURIFICATION TECHNOLOGY
(2021)
Review
Biotechnology & Applied Microbiology
Alonso Ornelas-Gonzalez, Mirna Gonzalez-Gonzalez, Marco Rito-Palomares
Summary: Stem cell-based therapies have emerged as an alternative for treating various diseases, with the use of microcarrier culture techniques showing promise for expanding stem cells. However, challenges in production and medical applications remain, highlighting the need for the development of new bioprocesses to simplify stem cell cultures.
CRITICAL REVIEWS IN BIOTECHNOLOGY
(2021)
Review
Biotechnology & Applied Microbiology
Alonso Ornelas-Gonzalez, Karolina Chairez-Cantu, Margarita Ortiz-Martinez, Mirna Gonzalez-Gonzalez, Marco Rito-Palomares
Summary: Stem cells have the potential to be used in cell therapy to treat degenerative diseases, with plant-derived compounds proposed as alternatives to animal-derived growth factors in cell culture. Plant-derived compounds are rich in bioactive substances with mainly anti-inflammatory, anti-oxidative, and immunomodulatory mechanisms. These compounds act as regulators of signaling pathways involved in stem cell proliferation and provide a lower-cost alternative with greater reproducibility in promoting cell proliferation under in vitro conditions.
JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY
(2021)
Review
Biotechnology & Applied Microbiology
Karolina Chairez-Cantu, Alonso Ornelas-Gonzalez, Margarita Ortiz-Martinez, Mirna Gonzalez-Gonzalez, Marco Rito-Palomares
Summary: Plant derivatives act as growth factors that can modulate or promote cell differentiation, which could lead to better treatment outcomes when used to supplement stem cell culture.
JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Mirna Gonzalez-Gonzalez, Guillermo Pico, Alvaro Silva Lima, Cassamo Ussemane Mussagy, Jorge Fernando Brandao Pereira, Hector Marcelo Fernandez-Lahore, Juan A. Asenjo, Marco Rito-Palomares
Summary: Aqueous two-phase systems (ATPS) are a simple and robust bioprocessing technology with high efficiency for concentration, enrichment, recovery, and purification of biological molecules. Four relevant countries in Latin America have played important roles in the practical evolution of ATPS. Novel applications of ATPS are being explored for visionary therapeutic tasks in healthcare, biotechnology, pharmaceutical sectors, and environmental purposes.
JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Mirna Gonzalez-Gonzalez, Marco Rito-Palomares
Summary: Bioseparation is a crucial process in obtaining biological products, with researchers focusing on optimizing upstream and downstream processes leading to a technological revolution. This perspective article summarises recent advances, challenges, and future trends in bioseparation, and proposes a classification of different technologies.
JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY
(2022)
Review
Biotechnology & Applied Microbiology
Calef Sanchez-Trasvina, Miguel Flores-Gatica, Daniela Enriquez-Ochoa, Marco Rito-Palomares, Karla Mayolo-Deloisa
Summary: Proteins with biorecognition properties are widely used as therapeutic agents, and can be modified to improve pharmacokinetic properties and stability. Purification steps are crucial for ensuring the quality and safety of the final product, with chromatographic methods being the gold standard for downstream processing.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Alonso Ornelas-Gonzalez, Margarita Ortiz-Martinez, Mirna Gonzalez-Gonzalez, Marco Rito-Palomares
Summary: Saliva has emerged as a non-invasive and valuable source of biomarker information, containing many of the biomarkers found in blood. While there are opportunities for improvement in terms of cost and complexity, enzymatic assays offer a cost-effective alternative for biomarker quantification in saliva samples. Critical analysis of technical elements for large-scale clinical applications of enzymatic methods is essential for overcoming current limitations.
Article
Pharmacology & Pharmacy
Alejandro Gonzalez-Mora, Kenny Misael Calvillo-Rodriguez, Jesus Hernandez-Perez, Marco Rito-Palomares, Ana Carolina Martinez-Torres, Jorge Benavides
Summary: The study developed a candidate phage-based vaccine displaying a cattle tick antigen, which induced both humoral and cellular immune-specific responses in bovine and murine models. This represents a promising alternative for controlling cattle tick infestations, though further evaluation in a bovine model is necessary.
Review
Endocrinology & Metabolism
Margarita Ortiz-Martinez, Mirna Gonzalez-Gonzalez, Alexandro J. Martagon, Victoria Hlavinka, Richard C. Willson, Marco Rito-Palomares
Summary: Diabetes mellitus is a complex chronic illness characterized by elevated blood glucose levels, with poor control leading to various complications. Type 2 diabetes represents the most common form and its incidence is increasing globally, particularly among children and young adults due to lifestyle factors. Early detection through cost-effective screening strategies and development of new biomarkers is crucial to prevent life-threatening complications.
CURRENT DIABETES REPORTS
(2022)
Article
Biotechnology & Applied Microbiology
Alonso Ornelas-Gonzalez, Mirna Gonzalez-Gonzalez, Marco Rito-Palomares
Summary: The study demonstrates the flexibility of an enzyme-based colorimetric system for the accurate quantification of various clinically relevant biomolecules. The optimized system shows excellent linearity, precision, and sensitivity, with low variability and high recovery rates. This multi-enzyme platform has great potential for quantifying different substrates in non-conventional biofluids.
JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY
(2022)
Editorial Material
Biotechnology & Applied Microbiology
Marco Rito-Palomares, Guillermo Quijano
JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY
(2022)