Article
Chemistry, Applied
Junxin Zhao, Maomao Ma, Xianghui Yan, Dongman Wan, Zheling Zeng, Ping Yu, Deming Gong
Summary: The study successfully immobilized lipase on functionalized magnetic nanocomposites, showing higher stability and activity. The immobilized lipase exhibited good activity and stability under high temperature and alkaline conditions.
Article
Biotechnology & Applied Microbiology
Yuan Ji, Zhengzhang Wu, Peng Zhang, Meng Qiao, Yi Hu, Baoxing Shen, Bingzhi Li, Xing Zhang
Summary: The study successfully encapsulated lipase and magnetic nanoparticles into ZIF-8 composite material using a mild and quick one-pot strategy, achieving high immobilization yield. The resulting magnetic MOF composite demonstrated excellent resistance to harsh environments, simplifying complex downstream operations, and maintaining high activity even after multiple reuse cycles.
BIOCHEMICAL ENGINEERING JOURNAL
(2021)
Article
Electrochemistry
Davino M. Andrade Neto, Luelc S. da Costa, Camila P. Sousa, Helena Becker, Paulo N. S. Casciano, Helio O. Nascimento, Joao R. Bezerra Neto, Pedro de Lima-Neto, Ronaldo F. Nascimento, Jhonyson A. C. Guedes, Raissa C. de Oliveira, Davila Zampieri, Adriana N. Correia, Pierre B. A. Fechine
Summary: Functionalized magnetic nanoparticles were used to modify glassy carbon electrodes for the development of electrochemical sensors. The electrooxidation reaction pathway of carbendazim was proposed based on electrochemical measurements and mass spectrometry. The proposed sensor exhibited low detection and quantitation limits, as well as stability and anti-interference ability.
ELECTROCHIMICA ACTA
(2022)
Article
Chemistry, Multidisciplinary
Alina Gabriela Rusu, Aurica P. Chiriac, Loredana Elena Nita, Vera Balan, Alexandru Mihail Serban, Alexandra Croitoriu
Summary: This study aims to determine the possibility of substituting GA with SA as a crosslinking agent in the process of enzyme immobilization. A comparison between SA- and GA-functionalized magnetic nanoparticles was conducted, and the results showed that the SA-functionalized MNPs had better stability and catalytic performance compared to the GA-modified MNPs. Therefore, they have greater potential as catalytic nanodevices for biomedical purposes.
Review
Biochemistry & Molecular Biology
Rafael Leandro Fernandes Melo, Misael Bessa Sales, Viviane de Castro Bizerra, Paulo Goncalves de Sousa Junior, Antonio Luthierre Gama Cavalcante, Tiago Melo Freire, Francisco Simao Neto, Muhammad Bilal, Teofil Jesionowski, Joao Maria Soares, Pierre Basilio Almeida Fechine, Jose Cleiton Sousa dos Santos
Summary: Magnetic biocatalysts, combining magnetic properties and enzyme catalytic activity, offer easy recovery and reuse. Lipases immobilized by magnetic nanoparticles dominate research in this field. This review provides a bibliometric analysis and overview of the area, highlighting research advances and hotspots, as well as the diverse applications of magnetic biocatalysts.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Biophysics
Hongbo Suo, Moju Li, Renmin Liu, Lili Xu
Summary: In this study, imidazolium-based ionic liquid was successfully grafted to magnetic polydopamine nanoparticles for lipase immobilization. The immobilized lipase showed excellent activity and stability, with increased affinity to substrate. Additionally, the immobilized lipase can be easily separated from the reaction system with a magnet, providing new ideas for further studies in this field.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2021)
Article
Biotechnology & Applied Microbiology
Shushu Wang, Shan Li, Runtang Liu, Wei Zhang, Huajin Xu, Yi Hu
Summary: In this study, Candida rugosa lipase (CRL) was activated and covalently immobilized on a nanocomposite material using surfactants. The nanocomposite material, fabricated by combining magnetic nanoparticles with chitosan, proved to be an ideal support for enzyme immobilization. The immobilized CRL showed improved stability and reusability.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Nanoscience & Nanotechnology
Jiawei Lu, Youran Li, Huilin Zhu, Guiyang Shi
Summary: The study successfully synthesized magnetic hollow composite beads with nanoscale porous structures for enzyme immobilization, demonstrating improved stability and functionality compared to traditional methods. The immobilized lipase in the composite beads showed higher thermal stability, resistance to alkali, and reusability in ester hydrolysis cycles, making it a promising candidate for industrial applications.
ACS APPLIED NANO MATERIALS
(2021)
Article
Engineering, Chemical
Xuewu Liu, Xingfei Li, Yuxiang Bai, Xing Zhou, Long Chen, Chao Qiu, Cheng Lu, Zhengyu Jin, Jie Long, Zhengjun Xie
Summary: This study presents a simple method to immobilize beta-agarase onto amino-modified magnetic nanoparticles using biotin/streptavidin recognition. The length of PEG played a crucial role in the catalytic performance of the immobilized enzyme, with longer PEG (PEG(24)) improving the stability and activity retention of the enzyme. The introduction of PEG(24) significantly enhanced the stability of beta-agarase and increased the yield of neoagarobiose.
INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Hui Guo, Bingshuang Lei, Jianwei Yu, Yunfei Chen, Junqing Qian
Summary: The study successfully immobilized Rhizopus lipase on magnetic dialdehyde cellulose nanoparticles, optimizing the immobilization conditions to improve enzyme stability and activity. Changes in the secondary structures of the immobilized enzyme revealed increased conformational rigidity, leading to enhanced stability at different temperatures and pH levels, as well as improved tolerance to organic reagents. After storage for 30 days and 6 cycles, the immobilized enzyme still retained high recovery activity, indicating the promising application prospect of MDC in immobilized enzymes.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Materials Science, Biomaterials
Yasemin Ispirli Dogac, Gokmen Arabaci, Hanife Erden, Funda Ozturk
Summary: In this study, NiFe2O4/Polyhydroxymethyl methacrylate (NF-pHEMA) and Fe3O4/Polyhydroxymethyl methacrylate (M-pHEMA) magnetic nanogel structures were optimized and characterized, and their usability for immobilizing lipase enzyme was investigated. The synthesized magnetic nanogels showed improved thermal and pH stability compared to the free enzyme, with lipase-immobilized M-pHEMA losing 50% of its activity after 15 uses and lipase-immobilized NF-pHEMA after 16 uses. The results indicate that immobilization onto magnetic nanogels enhances the properties of the lipase enzyme, suggesting potential applications in drug delivery systems.
INTERNATIONAL JOURNAL OF POLYMERIC MATERIALS AND POLYMERIC BIOMATERIALS
(2023)
Article
Engineering, Chemical
Saphan O. Akiri, Markus Schmitz, Stephen O. Ojwach
Summary: This paper describes the use of immobilized palladium catalysts on Fe3O4 magnetic nanoparticles for the methoxycarbonylation of 1-hexene. The immobilized Pd(II) complexes showed good catalytic activity and could be recycled multiple times without significant loss.
Article
Biochemistry & Molecular Biology
Maria Belen Abdulhamid, Johan Sebatian Hero, Mariana Zamora, Maria Ines Gomez, Maria Carolina Navarro, Cintia Mariana Romero
Summary: The research investigates the immobilization of lipase using adsorption on magnetic nanoparticles, cross-linked enzyme aggregates (CLEA), and their combination. The study highlights the importance of pH and ionic strength in the immobilization process and shows that the use of functionalized support leads to the best activity performance.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Biochemistry & Molecular Biology
Moju Li, Xusheng Dai, Aifeng Li, Qi Qi, Wenhui Wang, Jia Cao, Zhenting Jiang, Renmin Liu, Hongbo Suo, Lili Xu
Summary: In this study, an ionic liquid-modified magnetic metal-organic framework composite was prepared and used as a support for enzyme immobilization. The immobilized enzyme showed higher catalytic activity, thermal stability, and anti-denaturant properties compared to soluble enzymes. The results demonstrated that the ionic liquid-modified Fe3O4@UiO-66-NH2 has great potential for immobilized enzymes.
Article
Chemistry, Physical
Elif Ozyilmaz, Sebahat Ascioglu, Mustafa Yilmaz
Summary: Immobilization of enzymes to magnetic metal-organic frameworks enhances enzyme activity, stability, and reusability, with excellent enantioselectivity demonstrated. It may have implications for pharmaceutical industry due to increased reusability and stability, enantioselectivity exhibited by MOF-enzyme biocomposites, and significant differences in biological activities of enantiomers.
Article
Biology
Masoomeh Fooladi, Alireza Shirazi, Peyman Sheikhzadeh, Mahsa Amirrashedi, Fatemeh Ghahramani, Mohsen Cheki, Mehdi Khoobi
Summary: This study investigated the efficacy of telmisartan in the recovery process of intestinal tissue after in vivo irradiation using F-18-FDG microPET imaging. The results showed that telmisartan effectively attenuated radiation-induced injury in the intestine, which was consistent with histological analysis.
INTERNATIONAL JOURNAL OF RADIATION BIOLOGY
(2023)
Article
Polymer Science
Farzaneh Sorouri, Pegah Azimzadeh Asiabi, Parastoo Hosseini, Ali Ramazani, Sahar Kiani, Tayebeh Akbari, Mohammad Sharifzadeh, Marzieh Shakoori, Alireza Foroumadi, Loghman Firoozpour, Mohsen Amin, Mehdi Khoobi
Summary: This study introduces a simple strategy for preparing a multifunctional dressing for burn wound treatment using carnosine-enriched bentonite loaded into Carbopol gel. The results suggest that the Carb-CEB gel has improved physicochemical properties and antioxidant activity, leading to accelerated wound healing, tissue repair, and angiogenesis.
Article
Chemistry, Multidisciplinary
Saeed Babaee, Mohammad Ali Zolfigol, Gholamabbas Chehardoli, Mohammad Ali Faramarzi, Somayeh Mojtabavi, Tahmineh Akbarzadeh, Roshanak Hariri, Arezoo Rastegari, Farshad Homayouni Moghadam, Mohammad Mahdavi, Zahra Najafi
Summary: A new class of indolotacrine hybrids was designed, synthesized, and assessed as acetylcholinesterase inhibitors (AChEIs). Some of the derivatives showed good inhibitory effect against acetylcholinesterase. The cyclopenta-indolotacrine hybrids demonstrated slightly better anti-AChE activity than cyclohexa-indolotacrine hybrids. Compound 8g showed the best AChE inhibitory activity and acted as a competitive inhibitor.
JOURNAL OF THE IRANIAN CHEMICAL SOCIETY
(2023)
Article
Biology
Hossein Rabbi Angourani, Armin Zarei, Maryam Manafi Moghadam, Ali Ramazani, Andrea Mastinu
Summary: Phytochemicals from three different Achillea genera were analyzed for their interactions with the SARS-CoV-2 main protease. Their antiviral potential against both SARS-CoV-2 and SARS-CoV-1 main proteases was investigated. The study identified specific compounds that bind to the active site of the coronaviruses, blocking their progression. The findings suggest a potential for further preclinical studies on natural inhibitors of the main SARS-CoV-2 protease.
Review
Multidisciplinary Sciences
Ali Bodaghi, Nadia Fattahi, Ali Ramazani
Summary: The use of biomarkers as early warning systems in disease risk evaluation has greatly increased in the last decade. Biomarkers are indicators of typical biological processes, pathogenic processes, or pharmacological reactions to therapy. The application and identification of biomarkers in the medical and clinical fields have had a tremendous impact on society. This review discusses the history, definitions, classifications, characteristics, and discovery of biomarkers, as well as their potential application in the diagnosis, prognosis, and treatment of various diseases over the last decade. It aims to inspire readers to explore new avenues in biomarker research and development.
Article
Multidisciplinary Sciences
Mina Saeedi, Roshanak Hariri, Aida Iraji, Ali Ahmadi, Somayeh Mojtabavi, Shiva Golshani, Mohammad Ali Faramarzi, Tahmineh Akbarzadeh
Summary: In this study, various N'-substituted benzylidene benzohydrazide-1,2,3-triazoles were designed, synthesized, and evaluated for their inhibitory activity against alpha-glucosidase. The structures of the derivatives were confirmed using multiple spectroscopic techniques. All derivatives exhibited potent inhibition, with IC50 values ranging from 0.01 to 648.90 μM, compared to acarbose as the positive control (IC50 = 752.10 μM). Compounds 7a and 7h showed significant potency, with IC50 values of 0.02 and 0.01 μM, respectively. Further studies revealed that these compounds are noncompetitive inhibitors of alpha-glucosidase. Fluorescence quenching and molecular docking were also used to investigate the interaction and binding mechanisms between the inhibitors and the target enzyme.
SCIENTIFIC REPORTS
(2023)
Article
Multidisciplinary Sciences
Sara Moghadam Farid, Milad Noori, Mohammad Nazari Montazer, Minoo Khalili Ghomi, Marjan Mollazadeh, Navid Dastyafteh, Cambyz Irajie, Kamiar Zomorodian, Seyedeh Sara Mirfazli, Somayeh Mojtabavi, Mohammad Ali Faramarzi, Bagher Larijani, Aida Iraji, Mohammad Mahdavi
Summary: Different s-substituted benzimidazole-thioquinoline derivatives were synthesized and evaluated for their potential alpha-glucosidase inhibitory activities. The most active compound, 6j (X = 4-bromobenzyl), exhibited significant potency with an IC50 value of 28.0 +/- 0.6 μM, compared to the positive control acarbose with an IC50 value of 750.0 μM. Molecular dynamics simulations and in silico pharmacodynamics and ADMET properties were performed to assess the interactions and druggability of the derivatives. Overall, these derivatives show promise as potential alpha-glucosidase inhibitors.
SCIENTIFIC REPORTS
(2023)
Article
Chemistry, Multidisciplinary
Mahtab Rahmani Gavanrudi, Mohammad Halimi, Hossein Najafzadehvarzi, Majid Alikhani, Ali Moazzam, Soghra Farzipour, Samanesadat Hosseini, Somayeh Mojtabavi, Mohammad Ali Faramarzi, Ensieh Nasli-Esfahani, Maryam Mohammadi-Khanaposhtani, Mohammad Mahdavi
Summary: Thirteen new derivatives of phthalimide-1,2,3-triazole-N-phenylacetamide hybrids were synthesized and evaluated for their inhibitory potential against a-glucosidase. Seven compounds showed excellent inhibition (IC50 values = 1.30-31.7 μM) compared to the positive control acarbose (IC50 value = 750.1 μM), with the compound containing a 2,4-dichloro substituent on the phenyl ring being the most potent inhibitor. Molecular docking and dynamics studies were conducted to understand the interaction mode, stability, and flexibility of the most potent compounds, and in silico studies predicted their drug-likeness, pharmacokinetic, and toxicity properties.
Article
Environmental Sciences
Ghazaleh Royatvand-Ghiyasvand, Somayeh Mojtabavi, Anita Azmi, Hoda Jahandar, Mohammad Ali Faramarzi
Summary: From an environmental perspective, azo dye industrial effluent poses a significant risk to public health due to its toxic, carcinogenic, and teratogenic properties. However, enzyme-based technologies offer a promising and controllable method for removing synthetic dyes from wastewater. This study demonstrates the synthesis of hybrid nanoparticles (HNPs) with laccase as the catalyst, showing efficient removal of azo dyes and potential for wastewater treatment. Evaluation: 8 out of 10.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2023)
Article
Chemistry, Multidisciplinary
Sajedeh Safapoor, Mohammad Halimi, Minoo Khalili Ghomi, Milad Noori, Navid Dastyafteh, Shahrzad Javanshir, Samanesadat Hosseini, Somayeh Mojtabavi, Mohammad Ali Faramarzi, Ensieh Nasli-Esfahani, Bagher Larijani, Azadeh Fakhrioliaei, Mohammad G. Dekamin, Maryam Mohammadi-Khanaposhtani, Mohammad Mahdavi
Summary: A new series of quinoline-quinazolinone-thioacetamide derivatives 9a-p were designed and synthesized. These compounds showed significant anti-a-glucosidase activity, with compound 9g being the most potent inhibitor. Molecular simulation studies revealed that compound 9g occupied the active site of a-glucosidase with favorable binding energy. In silico ADMET studies of the most potent compounds predicted their drug-likeness, pharmacokinetic, and toxicity properties.
Article
Chemistry, Multidisciplinary
Sobhan Rezayati, Fatemeh Kalantari, Ali Ramazani
Summary: A green and facile method was proposed for designing a catalyst with Ni(ii) attached to a picolylamine complex on 1,3,5-triazine-immobilized Fe3O4 magnetic nanoparticles. The synthesized nanocatalyst had a high specific area and mesoporous structure, with a particle size distribution of 23-33 nm. The successful attachment of Ni(ii) on the surface of the nanoparticles was confirmed by XPS analysis. The catalyst exhibited high activity and recyclability in the synthesis of pyridine derivatives, achieving a yield of 97% and TOF of 823 h(-1).
Article
Nanoscience & Nanotechnology
Aram Rezaei, Huajun Zheng, Shiva Majidian, Saadi Samadi, Ali Ramazani
Summary: This study reports a chiral pseudohomogeneous catalyst that combines the advantages of both homogeneous and heterogeneous catalysts to control the enantioselectivity of the Kharasch-Sosnovsky reaction. The impact of various factors on the allylic oxidation reaction is investigated, and the catalyst is shown to be readily recyclable, making it promising for practical applications.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Chemistry, Applied
Farzaneh Moradnia, Saeid Taghavi Fardood, Ali Ramazani
Summary: In this study, NiFe2O4@ZnMn2O4 magnetic nanocomposites (MNCs) were successfully synthesized using a simple green sol-gel method. The MNCs were comprehensively characterized using various analytical techniques. The results showed that the MNCs exhibited good catalytic activity in the production of tetrahydropyrimidine and polyhydroquinoline derivatives, and their efficacy remained stable after four cycles.
APPLIED ORGANOMETALLIC CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Mahdiyeh Partovi, Sobhan Rezayati, Ali Ramazani, Yavar Ahmadi, Hooman Taherkhani
Summary: In this study, an efficient and magnetically recoverable nanocatalyst was successfully synthesized by modifying the surface of magnetic nanoparticles with silica and GPTMS, and treating it with the antioxidant mesalamine. The nanocatalyst exhibited high thermal stability and magnetization properties, and was used in the tandem Knoevenagel-Michael cyclocondensation to produce 2-amino-4H-benzo[b]pyran derivatives with good yields. The method offers several advantages, including gram-scale synthesis, good green chemistry metrics, easy fabrication of the catalyst, atom economy, no use of column chromatography, and avoidance of toxic materials.
Article
Chemistry, Applied
Maryam Keykhaee, Mahban Rahimifard, Alireza Najafi, Maryam Baeeri, Mohammad Abdollahi, Fatemeh Mottaghitalab, Mehdi Farokhi, Mehdi Khoobi
Summary: The study proposes a novel approach using a multifunctional hydrogel to manage diabetic foot ulcers (DFUs). The hydrogel, made from a combination of alginate and gum arabic, is enhanced with nerve growth factor (NGF) immobilized in specially designed mesoporous silica nanoparticles (MSN). The hydrogel shows sustained release of NGF and significantly improves wound healing. The hydrogel also reduces inflammation, increases angiogenesis, and improves tissue pathology. This newly developed composite system holds promise as a potential treatment for non-healing diabetic wounds.
CARBOHYDRATE POLYMERS
(2023)
