Review
Environmental Sciences
Thivaharan Varadavenkatesan, Shraddha Pai, Ramesh Vinayagam, Arivalagan Pugazhendhi, Raja Selvaraj
Summary: Aqueous two-phase system (ATPS) is a technique that is increasingly being recognized for its ability to improve yield and purity in a single step, making it a preferred choice for concentrating, separating, and purifying target products from industrial waste discharges. Novel approaches and optimization techniques are being actively explored to enhance the yield and purity of ATPS, with a growing focus on drawing attention from academia and industries towards developing innovative tweaks to current practices in ATPS.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Article
Chemistry, Multidisciplinary
Thi D. Ta, A. Kiet Tieu, Bach H. Tran
Summary: This research investigates the adsorption behaviors of two monomers of triblock copolymers on a TiO2 surface in aqueous solution using a multiscale theoretical framework. The study shows that the adsorption of these monomers on a hydroxylated surface is energetically more favorable than on a pure oxide surface. Different adsorption behaviors between the two monomers are observed due to the covalent bonding with the TiO2 surface. The presence of water affects the adsorption process, with 1,2-DME exhibiting weaker adsorption compared to 1,2-DMP.
Article
Polymer Science
Uddhab Kalita, Sarthik Samanta, Sovan Lal Banerjee, Narayan C. Das, Nikhil K. Singha
Summary: A novel thermoplastic elastomer (TPE) composed of polystyrene and poly(beta-myrcene) was successfully prepared using the RAFT polymerization method. This TPE showed good physical properties and sustainable recyclability.
Article
Polymer Science
Anabella A. Abate, Cristian M. Piqueras, Daniel A. Vega
Summary: When a cylinder forming triblock copolymer thin film is exposed to supercritical carbon dioxide, it undergoes an order-order phase transition from parallel to perpendicularly oriented cylinders. Defects modify the energetic barriers for nucleation during the phase transition, with topological defects in the primitive phase surviving the transition to provide a lower bound for the density of defects in the post-transitional phase. Control over the orientational order in the primitive phase can be used to manipulate order and defects in the transversely isotropic hexagonal pattern.
Article
Chemistry, Multidisciplinary
Tengfei Miao, Xiaoxiao Cheng, Gong Zhang, Yuqing Wang, Zixiang He, Zhao Wang, Wei Zhang
Summary: The controllable chiral microphase separation in an achiral amphiphilic block copolymer has been demonstrated, allowing for the fabrication of helical nanostructures. The chirality can be transferred to the nanostructures by doping with chiral additives, but can be completely dispensed with after their removal and cross-linking. These supramolecular chirality and microphase structures can be maintained under UV irradiation and heating-cooling treatment, enabling a reversible chiroptical switch feature. This work shows potential for applications in chiral separation and avoids the need for tedious synthesis and expensive raw materials.
Article
Chemistry, Physical
Qun Yu, De-chang Li, Zhengbin Tian, Chongzhi Zhu, Chengli Jiao, Qian Zhang, Yanping Chen, Yihan Zhu, Heqing Jiang, Jian Liu, Guang-Hui Wang
Summary: In this study, a facile and scalable method for synthesizing single-hole hollow carbon nanospheres (HCH) using poly(ethylene glycol) (PEG)-assisted emulsion templating is developed. The HCH exhibits higher adsorption capacities and faster adsorption rates, indicating the enhancement of liquid-phase adsorption induced by the single-hole hollow structure.
CHEMISTRY OF MATERIALS
(2022)
Article
Chemistry, Multidisciplinary
Sanyukta Bhattacharjee, Souvik Pandit, Debabrata Seth
Summary: This study characterizes the water dynamics of Poloxamer 407 (P-407) using spectroscopic techniques and investigates the effects of osmolytes on the critical micelle temperature (CMT) and polarity of the copolymer.
Article
Polymer Science
Berta Tinao, Juan L. L. Aragones, Laura R. R. Arriaga
Summary: This study uses microfluidic technologies to create vesicles that efficiently encapsulate mixtures of macromolecules, providing a versatile platform to determine the phase behavior of aqueous two-phase systems (ATPSs). It is found that a membrane selectively permeable to one component of the ATPS allows for spontaneous reversal of phase separation. This dynamics could be utilized by cells to separate and connect metabolic and signaling routes depending on external conditions.
Review
Biochemistry & Molecular Biology
Lirong Nie, Ziwei Zheng, Mingxia Lu, Shun Yao, Dong Guo
Summary: This article summarizes the phase behavior of IL-based ATPSs in chemical reactions and separation processes, and highlights the possible future directions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Polymer Science
Niamh Bayliss, Gokhan Yilmaz, Alexander Plucinski, C. Remzi Becer, Bernhard V. K. J. Schmidt
Summary: Aqueous two-phase systems (ATPS) have gained attention in polymer chemistry due to their biocompatibility and applications in biomaterials and biology. This study investigated ATPS formed from poly(N,N-diethylacrylamide) (PDEA) with dextran and poly(ethylene glycol), and observed a significant shift in PDEA's cloud point temperature in the ATPS compared to pure aqueous solutions. This effect on thermoresponsive behavior opens up new possibilities for water-based polymer systems in areas such as multicompartment hydrogels or biotechnology.
Article
Engineering, Chemical
Tian Yao, Hongmei Li, Yuanhang Ren, Meilin Feng, Yichen Hu, Huiling Yan, Lianxin Peng
Summary: The report introduces thermo-separating magnetic ionic liquids (MILs) for the first time, detailing the synthesis, characterization, and application of two PPG600-based thermo-separating MILs, which were used to create a novel MILATPS system. The system showed high extraction efficiencies for phenolic compounds, exceeding 90%, and demonstrated potential for industrial treatment of phenolic wastewater with efficient recovery processes.
SEPARATION AND PURIFICATION TECHNOLOGY
(2022)
Article
Environmental Sciences
Pixiang Wang, Sarah Tyndall, Tawsif Rahman, Poulami Roy, Hossein Jahromi, Sushil Adhikari, Melissa Boersma
Summary: This study investigated the sorption of phenolic compounds by bio-char and found that the pseudo-second-order kinetic model described the experimental results well. The Langmuir isotherm model was suitable at high sorption temperatures, while the Freundlich isotherm model was better at low temperatures. Additionally, alkaline desorption and regeneration experiments were conducted, and the eluents with phenolic compounds were characterized using a liquid chromatography-mass spectrometer.
Article
Engineering, Chemical
Yuqiu Chen, Xianglei Meng, Yingjun Cai, Xiaodong Liang, Georgios M. Kontogeorgis
Summary: A machine learning-based method was proposed to design and optimize ionic liquid-based aqueous biphasic systems, achieving efficient recovery of hydrophilic ILs. Through the combination of machine learning models and optimization algorithms, the recovery efficiency was improved and the usage of salting-out agents was reduced.
INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
(2021)
Article
Food Science & Technology
Kar Yeen Chong, Yury Yuryev, Abhinav Jain, Beth Mason, Marianne Su-Ling Brooks
Summary: Pea protein films incorporating haskap leaf extracts from aqueous two-phase systems showed improved water vapor permeability and flexibility, along with thermal stability and UV protection. The films also demonstrated potential for extending shelf life and enhancing antioxidant properties of packaged products, without the need for additional processing steps, thus reducing costs.
FOOD AND BIOPROCESS TECHNOLOGY
(2021)
Article
Chemistry, Physical
Guilherme Max Dias Ferreira, Maria C. Hespanhol, Luis H. M. da Silva, Pamela R. Patricio, Roselaine C. da Cunha
Summary: Polymer-electrolyte aqueous biphasic systems (ABS) are environmentally and economically sustainable alternatives for metal extraction. The efficiency of metal extraction depends on the hydrophobicity and size of the polymer, electrolyte structure, and pH. Fe(III) and Co(II) can be extracted at high rates up to 100% and 97%, respectively, without the use of any auxiliary complexing agent in ABS.
JOURNAL OF MOLECULAR LIQUIDS
(2021)
Review
Food Science & Technology
Cassamo U. Mussagy, Jorge F. B. Pereira, Laurent Dufosse, Vijaya Raghavan, Valeria C. Santos-Ebinuma, Adalberto Pessoa Jr
Summary: This article reviews the main methods for producing Astaxanthin (AXT) using the yeast Phaffia rhodozyma strains, emphasizing the impact of conventional and non-conventional procedures for extracting AXT from yeast cells. Furthermore, future research directions are identified, including the use of low-cost residues to improve the economic and environmental sustainability of the bioprocess, the use of environmentally-friendly and low-energy operations for the extraction and purification of AXT, as well as the need for further human clinical trials using yeast-based AXT.
CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION
(2023)
Article
Biochemical Research Methods
Henrique P. Biasoto, Cristina B. Hebeda, Sandra H. P. Farsky, Adalberto Pessoa, Tales A. Costa-Silva, Gisele Monteiro
Summary: L-asparaginase (ASNase) is an effective inhibitor of tumor development and is used in chemotherapy for acute lymphoblastic leukemia. L-asparaginase II from Saccharomyces cerevisiae shows potential to replace bacterial ASNase for patients with hypersensitivity. In this study, we describe a genetic process to produce extracellular ScASNaseII, which exhibits higher stability and lower cross-reaction with antibodies compared to commercially available ASNase.
PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Lucas Silva de Sousa, Flaviana S. Chaves, Rafael B. Ferraro, Adalberto Pessoa Jr, Luis Antonio Minim
Summary: In this study, a supermacroporous cryogel activated with polyethyleneimine and quaternized with epichlorohydrin was used to capture L-asparaginase from unclarified broth. The activated cryogel showed a ligand density of 121.6 mmol/L and the HETP ranged from 0.18 to 0.27 cm. Optimum yield and purification fold were achieved at 56% and 7.6, respectively, through response-surface methodology optimization. The activated cryogel exhibited high convective mass transfer property and can be used with high flow rate to reduce unit production cost.
PROCESS BIOCHEMISTRY
(2023)
Article
Biotechnology & Applied Microbiology
Cassamo U. Mussagy, Fabiane O. Farias, Valeria C. Santos-Ebinuma, Jorge F. B. Pereira, Adalberto Pessoa Jr
Summary: Microbial-based pigments, such as astaxanthin and β-carotene produced by P. rhodozyma, have attracted attention as greener and more effective alternatives in the cleaning and cosmeceutical industries. However, the optimization of downstream processing operations for carotenoid recovery from P. rhodozyma has not been fully achieved. This study proposes a sustainable one-pot process using biocompatible and renewable solvents for the recovery of astaxanthin and β-carotene from P. rhodozyma biomass and formulation of bioactive soaps.
ENVIRONMENTAL TECHNOLOGY & INNOVATION
(2023)
Article
Biochemistry & Molecular Biology
Alejandro Pedroso, Lisandra Herrera Belen, Jorge F. F. Beltran, Rodrigo L. L. Castillo, Adalberto Pessoa, Enrique Pedroso, Jorge G. G. Farias
Summary: Acute lymphoblastic leukemia (ALL) is a common childhood cancer characterized by overproduction of undifferentiated lymphoblasts. The enzyme L-asparaginase (ASNase) from bacterial sources is the preferred treatment option for this disease. However, the current ASNase formulations have significant adverse effects, including immunogenicity. In this study, a humanized chimeric enzyme was developed by replacing the immunogenic epitopes of E. coli L-asparaginase with those of a less immunogenic Homo sapiens asparaginase, aiming to reduce immunological problems and improve patient safety.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Environmental Sciences
Higor Tulio Correa, William Fernando Vieira, Adalberto Pessoa Jr, Vicelma Luiz Cardoso, Ubirajara Coutinho Filho
Summary: Insects, especially Tenebrio molitor, are being explored as a potential source for the production of enzymes and biosurfactants due to their low cost and ease of production. This study investigated the production of l-asparaginase and biosurfactants using T. molitor biomass as the primary source, and the results showed promising outcomes.
WASTE AND BIOMASS VALORIZATION
(2023)
Article
Biotechnology & Applied Microbiology
Juan Gabriel Puentes, Soledad Mateo, Sebastian Sanchez, Ines C. Roberto, Alberto J. Moya
Summary: This study utilized hemicellulosic biomass from olive-tree pruning to produce a hemicellulosic hydrolysate for fermentation. The non-traditional yeast Candida guilliermondii FTI 20037 was used to obtain ethanol and xylitol. The research focused on studying relevant kinetic parameters and comparing different fermentation scenarios.
FERMENTATION-BASEL
(2023)
Article
Biotechnology & Applied Microbiology
Flavio Cardozo, Valker Feitosa, Omar Pillaca-Pullo, Adalberto Pessoa
Summary: This study demonstrates the potential to maximize the production of endochitinase (EnCh) and chitobiosidase (ChB) by supplementing nitrogen sources in the culture media of A. caviae CHZ306. The optimal conditions for enzyme production were observed at 12 h using corn-steep solids and peptone A, resulting in a 5-fold increase in EnCh activity and a 3-fold increase in ChB activity compared to the control condition.
BIOENGINEERING-BASEL
(2023)
Article
Microbiology
Flavio Augusto Cardozo, Valker Feitosa, Carlos Miguel Nobrega Mendonca, Francisco Vitor Santos da Silva, Attilio Converti, Ricardo Pinheiro de Souza Oliveira, Adalberto Pessoa Jr
Summary: In this study, GlcNAcase production by marine Aeromonas caviae CHZ306 was enhanced through optimization of medium composition and physicochemical conditions. The resulting increase in enzyme productivity provides a basis for future industrial applications.
BRAZILIAN JOURNAL OF MICROBIOLOGY
(2023)
Article
Agricultural Engineering
Alana M. M. Maia, Adalberto Pessoa-Junior, Ines C. Roberto
Summary: In this study, ferulic acid (FA) and p-coumaric acid (pCA) were extracted from rice straw using an aqueous two-phase system (ATPS) based on the cloud point of surfactant/copolymer Pluronic F-127 (PF-127). The extraction process resulted in 75.8% yield for FA and 74.3% yield for pCA at a concentration of 9.4% w/w of PF-127. Furthermore, the extracted FA and pCA were concentrated 2.3 and 2.5-fold, respectively, compared to their original concentrations, resulting in a PF-127 gel containing 0.12% w/w of hydroxycinnamic acids (HAs). This material shows potential for application in the cosmetics industry for high value-added products in rice straw-based biorefineries.
INDUSTRIAL CROPS AND PRODUCTS
(2023)
Article
Biochemistry & Molecular Biology
Iris Munhoz Costa, Brian Effer, Tales Alexandre Costa-Silva, Chen Chen, Michael F. Ciccone, Adalberto Pessoa, Camila O. dos Santos, Gisele Monteiro
Summary: L-Asparaginase (ASNase) is an important drug for the treatment of acute lymphoblastic leukemia (ALL), but some ALL cases are naturally resistant to ASNase treatment. The REH ALL cell line, which is resistant to ASNase treatment, was used to investigate the role of cathepsin B (CTSB) in ASNase resistance. The deletion of CTSB in REH ALL cells did not confer sensitivity to ASNase treatment, suggesting that intrinsic expression of CTSB is not a mechanism driving resistance in these ALL cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Pharmacology & Pharmacy
Samuel Leite Cardoso, Paula Monteiro Souza, Kelly Rodrigues, Isabella de Souza Mota, Edivaldo Ferreira Filho, Leia Cecilia de Lima Favaro, Felipe Saldanha-Araujo, Mauricio Homem-de-Mello, Adalberto Pessoa, Damaris Silveira, Yris Maria Fonseca-Bazzo, Perola Oliveira Magalhaes
Summary: The search for new drug-producing microorganisms is highly promising in the world of science today. This study successfully cloned and purified L-asparaginase from Fusarium proliferatum in Pichia pastoris, and analyzed its enzymatic activity and predicted its monomeric structure based on the analysis of amino acid residue positions.
Article
Chemistry, Multidisciplinary
Cassamo U. Mussagy, Fabiane O. Farias, Josana C. Sasaki, Mateus Scontri, Flavio Picheli, Valeria C. Santos-Ebinuma, Henriette M. C. de Azeredo, Adalberto Pessoa, Rondinelli D. Herculano
Summary: Food waste and consumer safety are important issues that require improved packaging systems. A new packaging material formulated with bio-based sodium alginate and choline-based deep eutectic solvents has been created to enhance food safety and longevity. Coated strawberries using this material showed longer shelf life, inhibited microbial proliferation, and had less weight loss.
MATERIALS TODAY CHEMISTRY
(2023)
Review
Oncology
Maria Tosta Perez, Lisandra Herrera Belen, Pablo Letelier, Yolanda Calle, Adalberto Pessoa, Jorge G. Farias
Summary: L-Asparaginase is an approved antileukemic drug for childhood acute lymphoblastic leukemia, but its efficacy and use have been debated due to adverse effects, rapid plasma elimination, multiple administrations, and allergic reactions. Finding less immunogenic variants has been a focus of study. This review presents the aspects of L-asparaginase enzyme from structural, pharmacological, and clinical perspectives in chemotherapy protocols with other drugs.
