Article
Cardiac & Cardiovascular Systems
Marilena Vitale, Kati Hanhineva, Ville Koistinen, Seppo Auriola, Jussi Paananen, Giuseppina Costabile, Giuseppe Della Pepa, Angela A. Rivellese, Gabriele Riccardi, Rosalba Giacco
Summary: This study found that in patients with metabolic syndrome, intake of wholegrain cereals led to an increase in lipid and phenolic compounds, which were associated with lower postprandial insulin and triglyceride responses. These observations suggest a potential role of lipid and polyphenol metabolites in the metabolic benefits of wholegrains in individuals at high risk of cardiovascular disease.
NUTRITION METABOLISM AND CARDIOVASCULAR DISEASES
(2021)
Article
Chemistry, Applied
Alexandra A. Crook, Diana Zamora-Olivares, Fatema Bhinderwala, Jade Woods, Michelle Winkler, Sebastian Rivera, Cassandra E. Shannon, Holden R. Wagner, Deborah L. Zhuang, Jessica E. Lynch, Nathan R. Berryhill, Ron C. Runnebaum, Eric Anslyn, Robert Powers
Summary: The study evaluated three important wine parameters - vineyard, region, and vintage year - by analyzing 15 Pinot noir wines using NMR spectroscopy and a peptide-based sensing array. By combining these techniques, the genetically identical wines were classified based on distinctive metabolic signatures associated with their growth region, vineyard, and vintage year.
Article
Biochemistry & Molecular Biology
Doaa B. Saied, Nehal S. Ramadan, Magdy M. El-Sayed, Mohamed A. Farag
Summary: This study aimed to assess metabolites' heterogeneity in different species and cultivars of five major cereal and leguminous seeds at different maturity stages. Nutrient profiling using gas chromatography mass-spectrometry analysis and sample segregation using chemometric tools were employed. The results revealed differences in nutrient composition among seeds at different maturity stages, providing a novel insight on the metabolomics of cereal and leguminous seeds.
Review
Chemistry, Multidisciplinary
Shi Qiu, Sifan Guo, Qiang Yang, Yiqiang Xie, Songqi Tang, Aihua Zhang
Summary: Metabolites are interrelated genotypes that can provide clear information about the final phenotype. The high-throughput analysis platform can quickly detect metabolic characteristics from large spectral data, which involves peak data preprocessing, statistical analysis, and functional interpretation. Developing a comprehensive strategy for discovering and verifying bioactive metabolites can provide a large number of new functional biomarkers, revealing their functional changes, and having biological significance for disease diagnosis and prognosis treatment.
FRONTIERS IN CHEMISTRY
(2023)
Article
Chemistry, Analytical
Elba Garreta-Lara, Cristian Gomez-Canela, Bruno Campos, Carlos Barata, Roma Tauler, Silvia Lacorte
Summary: This study utilized a combination of target and untargeted analytical approaches to assess the metabolic changes of Daphnia magna exposed to the endocrine disruptor bisphenol A. The results indicated that bisphenol A had an impact on the metabolism of Daphnia magna and potential biomarkers of exposure were discovered.
MICROCHEMICAL JOURNAL
(2021)
Article
Chemistry, Medicinal
Muhammad Safwan Ahamad Bustamam, Hamza Ahmed Pantami, Awanis Azizan, Khozirah Shaari, Chong Chou Min, Faridah Abas, Norio Nagao, Maulidiani Maulidiani, Sanjoy Banerjee, Fadzil Sulaiman, Intan Safinar Ismail
Summary: This study analyzed the metabolites of Isochrysis galbana using NMR and LCMS techniques, identifying diverse metabolites in the ethyl acetate and water extracts, including carotenoids, polyunsaturated fatty acids, and amino acids. Additionally, MS/MS data revealed similar metabolites between the two techniques, as well as the discovery of glycerophospholipids through a molecular network platform. The findings contribute to a better understanding of I. galbana's chemical composition and its potential as a feed ingredient in aquaculture.
Article
Chemistry, Applied
Yan Cui, Kunze Du, Shixin Hou, Rui Yang, Lina Qi, Jin Li, Yanxu Chang
Summary: This study used a strategy that integrated metabolomics with chemometrics to identify quality markers for distinguishing the function-related bioactivity of fruits. Hawthorn fruit was used as an example. The comprehensive process provided guidance for discovering function-related active markers for food assessment.
Article
Nutrition & Dietetics
Margherita Dall'Asta, Donato Angelino, Gaetana Paolella, Rossella Dodi, Nicoletta Pellegrini, Daniela Martini
Summary: The lack of a common legal definition for wholegrains leads to an unclear classification of products in grocery stores. This study analyzed the nutritional quality of wholegrain products compared to products made with partially wholegrain or refined grains in the Italian market. The results showed that the nutritional quality varied among different product categories and that the inclusion of wholegrains is not always a guarantee of overall nutritional quality.
Article
Food Science & Technology
Asmaa M. Otify, Shahira A. ElBanna, Basma M. Eltanany, Laura Pont, Fernando Benavente, Rana M. Ibrahim
Summary: This study characterized the metabolite profiles of six lettuce varieties and established their correlation with antivirulence effects. Various metabolites were identified, and red varieties showed higher levels of chlorogenic and chicoric acids indicating antioxidant properties. Romaine longifolia exhibited enrichment in amino acids and disaccharides, while crispa, capitata, and lolla bionda had a high content of β-carboline alkaloids. All varieties demonstrated antibacterial potential against Staphylococcus aureus strains, and specific bioactives such as sesquiterpenes, β-carboline alkaloids, amino acids, and oxy-fatty acids were identified. The results highlight the significance of lettuce as a functional food source and suggest breeding programs should focus on varieties rich in antibacterial agents.
FOOD RESEARCH INTERNATIONAL
(2023)
Article
Nutrition & Dietetics
Stefania Noerman, Jyrki K. Virtanen, Marko Lehtonen, Carl Brunius, Kati Hanhineva
Summary: The study aimed to identify fasting serum metabolites associated with whole grain intake in a free-living population. The findings suggested strong associations between whole grain intake and several metabolites, which remained significant even after adjusting for potential confounders. These metabolites have the potential to serve as robust biomarkers for whole grain consumption.
EUROPEAN JOURNAL OF NUTRITION
(2023)
Article
Food Science & Technology
Tamyris de Aquino Gondim, Jhonyson Arruda Carvalho Guedes, Maria Francilene Souza Silva, Adenilton Camilo da Silva, Ana Paula Dionisio, Fernanda Vidigal Duarte Souza, Claudia do O. Pessoa, Gisele Simone Lopes, Guilherme Juliao Zocolo
Summary: Pineapple is a widely produced and consumed fruit, resulting in a large amount of agricultural waste. This study aims to evaluate the chemical composition of pineapple leaves and explore their potential as a source of secondary metabolites and minerals. The results show similarities and differences in the chemical composition of pineapple leaves, and their extracts were found to be non-toxic.
FOOD RESEARCH INTERNATIONAL
(2023)
Review
Food Science & Technology
Dilek Eltemur, Peter Robatscher, Michael Oberhuber, Matteo Scampicchio, Alberto Ceccon
Summary: Nuclear magnetic resonance (NMR) spectroscopy is a promising technique for the analysis of bovine milk due to its non-destructive nature, minimal sample preparation requirements, and comprehensive approach. This review provides an overview of the applications of NMR techniques in assessing the quality and authenticity of bovine milk, highlighting key studies and emphasizing the versatility and significance of NMR spectroscopy in milk metabolomics research.
Article
Biochemistry & Molecular Biology
Mohamed A. Farag, Eman M. Kabbash, Ahmed Mediani, Stefanie Doll, Tuba Esatbeyoglu, Sherif M. Afifi
Summary: The present study used UPLC-MS and GC-MS to assess metabolite heterogeneity among four major Cinnamomum species. A new proanthocyanidin was identified for the first time in C. tamala, along with several glycosylated flavonoids and dicarboxylic fatty acids reported for the first time in cinnamon. Multivariate data analysis revealed differences in metabolite composition, including cinnamates, proanthocyanidins, dihydrocoumaroylglycosides, and coumarin, among the species. GC-MS analysis detected different primary metabolites, including sugars, fatty acids, and sugar alcohols, with true cinnamon from Malaysia suggested as a good sugar source for diabetic patients.
Article
Agricultural Engineering
Francesc Puig-Castellvi, Cedric Midoux, Angeline Guenne, Delphine Conteau, Oscar Franchi, Chrystelle Bureau, Celine Madigou, Delphine Jouan-Rimbaud Bouveresse, Pablo Kroff, Laurent Mazeas, Douglas N. Rutledge, Gilberte Gaval, Olivier Chapleur
Summary: The performance of full-scale anaerobic digesters can be regulated by modifying their operational conditions, but little is known about the impact of these modifications on their microbiome. This study used a multi-omics approach to investigate the effects of temperature modification on the microbial community of two mesophilic anaerobic digesters. The results showed that lowering the temperature resulted in an increase in sub-dominant bacterial populations, disrupting the microbial community equilibrium and reducing biogas production. However, the bacterial community showed resilience and recovered its structure and functional activity after restoring the initial mesophilic temperature.
BIORESOURCE TECHNOLOGY
(2022)
Article
Chemistry, Applied
Suyi Liu, Xingyue Jin, Rui Wang, Xue Meng, Kunze Du, Jin Li, Xiumei Gao, Yanxu Chang
Summary: This research evaluated the thrombin inhibition activities of perilla seeds by extracting samples using different polarity solvents. Metabolomics combined with chemometrics revealed the active markers and mechanisms of inhibiting thrombin in perilla seeds. The extraction process of six active markers was optimized using orthogonal experimental design. The results identified functional and active markers in perilla seeds, making it a potential antithrombin agent and a useful ingredient in the food industry. Metabolomics combined with chemometrics contributes to the discovery of active markers and accurate quality control in perilla seeds.
Article
Fisheries
Ashild Krogdahl, Elvis M. Chikwati, Aleksei Krasnov, Anusha Dhanasiri, Gerd M. Berge, Violetta Aru, Bekzod Khakimov, Soren Balling Engelsen, Hilde Vinje, Trond M. Kortner
Summary: This study aimed to investigate whether increased fishmeal levels and supplementation with choline, beta-glucan, and nucleotides could reduce the symptoms of steatosis and inflammation in Atlantic salmon fed plant rich diets. The results showed that increasing fishmeal levels and supplementation reduced steatosis but had no effect on inflammation. Choline level appeared to be related to these effects. The study also found that increasing fishmeal levels increased microbial richness and diversity in the gut, but only for unsupplemented diets. An average choline requirement of 3.5 g/kg was indicated for Atlantic salmon in the present life stage and condition.
AQUACULTURE NUTRITION
(2023)
Article
Chemistry, Applied
Li Ding, Wenxin Liang, Jianzhou Qu, Staffan Persson, Xingxun Liu, Klaus Herburger, Jacob Judas Kain Kirkensgaard, Bekzod Khakimov, Kasper Enemark-Rasmussen, Andreas Blennow, Yuyue Zhong
Summary: The study found that starch phosphate content (SPC) is an important parameter affecting the functionality of potato starch. A higher SPC is associated with more long amylopectin chains, lower double helical content and higher flexibility of the nano-lamellar structure. The phosphate monoesters, C-3 and C-6 phosphate, have different correlations with the average chain lengths of amylopectin chains and the structural characteristics of starch. These findings provide insights for potato breeding to control SPC and generate starch with different functionalities.
CARBOHYDRATE POLYMERS
(2023)
Article
Nutrition & Dietetics
Jacob Bulow, Bekzod Khakimov, Soren Reitelseder, Rasmus Bechshoft, Mikkel Jensen, Gerrit van Hall, Soren Balling Engelsen, Lars Holm
Summary: This study investigated the impact of 12 months of protein supplementation with or without physical exercise training on the basal and postprandial MPS and the skeletal muscle metabolome of healthy older Danes. The results showed that one year of protein supplementation had no effect on the MPS or the skeletal muscle metabolome of older individuals.
EUROPEAN JOURNAL OF NUTRITION
(2023)
Article
Fisheries
Ashild Krogdahl, Anusha K. S. Dhanasiri, Aleksei Krasnov, Violetta Aru, Elvis M. Chikwati, Gerd M. Berge, Soren Balling Engelsen, Trond M. Kortner
Summary: Functional feed ingredients are commonly used in feeds for Atlantic salmon to improve immune functions and reduce gut inflammation. However, their effectiveness is often uncertain. This study evaluated the effects of two packages of functional feed ingredients on inflammation models in salmon. The results showed that the inclusion of these ingredients did not significantly alleviate inflammation in salmon fed soybean meal, but they did alter the microbiota composition. The overall importance of this study is 9 out of 10.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Chemistry, Applied
Wenxin Liang, Li Ding, Ke Guo, Yang Liu, Xiaoxia Wen, Jacob Judas Kain Kirkensgaard, Bekzod Khakimov, Kasper Enemark-Rasmussen, Kim Henrik Hebelstrup, Klaus Herburger, Xingxun Liu, Staffan Persson, Andreas Blennow, Yuyue Zhong
Summary: This study investigated the multi-scale structural dynamics of amylopectin-only barley starch (APBS), normal barley starch (NBS), and amylose-only barley starch (AMBS) during enzymatic digestion. Rapid digestion stages followed by slow digestion stages were identified for all starches. During the rapid digestion stage, the content of short chains and the thickness of crystalline and amorphous nano-lamellae increased, while crystallinity decreased. The slow digestion stage was characterized by changes in branching degree, crystallinity, and structure loss.
FOOD HYDROCOLLOIDS
(2023)
Article
Chemistry, Applied
Zhenyu Liu, Yuyue Zhong, Bekzod Khakimov, Yongxia Fu, Tomasz Pawel Czaja, Jacob Judas Kain Kirkensgaard, Andreas Blennow, Qun Shen, Soren Balling Engelsen
Summary: This study investigates the effects of using High hydrostatic pressure (HHP) as a pre-treatment to promote Maltogenic alpha-amylase (MA) catalysis. The results show that HHP enhances the reactivity of MA by causing more indentations and fissures in starch granules. Furthermore, the combination of HHP and MA treatment increases the proportion of short malto-oligosaccharide chains with degrees of polymerization (DP) 1-9, while decreasing the proportion of longer chains with DP 13-24.
FOOD HYDROCOLLOIDS
(2023)
Article
Agriculture, Multidisciplinary
Shang Lin, Xinming Xu, Jesper Holck, Jane Wittrup Agger, Casper Wilkens, Zhuqing Xie, Bekzod Khakimov, Dennis S. Nielsen, Anne S. Meyer
Summary: Corn bran contains substituted glucuronoarabinoxylan polysaccharides that are cross-linked by diferulic acid moieties. Three enzymatically solubilized corn bran glucuronoarabinoxylans were assessed for their prebiotic activity. The results showed that these compounds have the potential to act as prebiotics, as they induced changes in the gut microbiome composition and stimulated short-chain fatty acid production.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Yongxin Ye, Jiahua Fan, Zhiteng Chen, Xiuwen Li, Maoxiong Wu, Wenhao Liu, Shiyi Zhou, Morten Arendt Rasmussen, Soren Balling Engelsen, Yangxin Chen, Bekzod Khakimov, Min Xia
Summary: This study aimed to identify plasma lipoproteins as potential biomarkers for assessing the severity of coronary lesions in UA patients. Ten important lipoproteins were identified using proton nuclear magnetic resonance spectroscopy. These lipoproteins showed significant discrimination power in distinguishing high GS UA patients from NCA patients.
Article
Chemistry, Applied
Paraskevi Tsermoula, Mie Rostved Bechshoft, Christoffer Friis, Soren Balling Engelsen, Bekzod Khakimov
Summary: Molecular profiling was carried out on side-streams and lactose powder generated from whey permeate production, revealing minor compounds that affect lactose crystallization and lactose powder properties. The analysis identified 110 compounds, with 49 of them quantified using GC-MS and 1H NMR spectroscopy. The study showed the composition of the side-streams changed during the unit operations, and certain compounds co-crystallized with lactose in the production process.
Article
Chemistry, Applied
Jose Manuel Amigo, Birthe Moller Jespersen, Frans van den Berg, Jorgen Juul Jensen, Soren B. Engelsen
Summary: The Danish buttered cookie is a well-known confectionery product that is produced using batch-wise and continuous dough mixing methods. This study analyzed the physicochemical differences of the cookies after baking and found that the changes in texture between the two production methods are mainly attributed to the difference in crystalline sucrose and the presence of migrated butter-fat spots on the surface of the cookies in continuous production. Near Infrared hyperspectral imaging and Chemometrics were used to conduct the analysis.
Article
Engineering, Chemical
Margherita Tonolini, Frans W. J. van den Berg, Peter Baek Skou, Klavs Martin Sorensen, Soren Balling Engelsen
Summary: The combination of near-infrared spectroscopy (NIRS) and chemometric techniques can be used for monitoring protein composition in industrial whey streams. Continuous monitoring using in-line NIRS allows for a comprehensive understanding of the protein fractionation process and provides valuable information for process optimization.
JOURNAL OF FOOD ENGINEERING
(2023)
Article
Chemistry, Multidisciplinary
Yong Zhao, Nikolaj L. Hansen, Yao-Tao Duan, Meera Prasad, Mohammed S. Motawia, Birger L. Moller, Irini Pateraki, Dan Staerk, Soren Bak, Karel Miettinen, Sotirios C. Kampranis
Summary: Researchers have identified a compound called celastrol in the roots of Tripterygium wilfordii, which has potential as an effective treatment for obesity. They have also developed a synthetic method for celastrol, allowing it to be produced from table sugar. This study demonstrates the successful combination of plant biochemistry, metabolic engineering, and chemistry for the scalable synthesis of complex specialized metabolites.
