Article
Chemistry, Multidisciplinary
Veronika M. Shoba, Dhanushka N. P. Munkanatta Godage, Santosh K. Chaudhary, Arghya Deb, Sachini U. Siriwardena, Amit Choudhary
Summary: Phosphorylation-inducing chimeric small molecules (PHICS) can enable protein kinase C (PKC) to have new activities, such as inducing inhibitory neo-phosphorylations on a variant and phosphorylating cytosol-based neo-substrates. PKC with PHICS can also induce death of chronic myeloid leukemia cells. Synthetic chemistry has the power to expand the chemical and functional diversity of proteins in cells using bifunctional molecules.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Article
Biochemistry & Molecular Biology
Jesus Bertran-Gonzalez, Caroline Dinale, Miriam Matamales
Summary: Multidisciplinary evidence suggests that the control of voluntary action is mediated by two major forms of behavioral processing: cognitively guided and autonomously guided. Brain-state irregularities, such as aging, often shift control towards the latter, but the neural mechanisms responsible are still unknown. By combining instrumental conditioning, cell-specific mapping, and chemogenetics, this study investigated strategies to enhance goal-directed control in aged mice. The findings suggest that desensitization of D2-SPN signaling in the striatum can shift behavior towards vigorous, goal-directed action and enhance cognitive functioning in habit-prone brains.
Article
Multidisciplinary Sciences
Kotaro Azuma, Kazuhiro Ikeda, Takashi Suzuki, Kenjiro Aogi, Kuniko Horie-Inoue, Satoshi Inoue, Tak W. Mak
Summary: The study highlights the role of TRIM47 in promoting breast cancer proliferation and endocrine therapy resistance by forming a complex with PKC-ε and PKD3. These findings suggest TRIM47 and its associated kinases as potential diagnostic and therapeutic targets for breast cancer refractory to endocrine therapy.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Multidisciplinary Sciences
Fushi Wang, Chunxiao Zhao, Pinlong Zhao, Fanfan Chen, Dan Qiao, Jiandong Feng
Summary: This study reports the direct identification of single amino acids in MoS2 nanopores, as well as the discrimination of amino acid isomers and phosphorylation. This is a significant breakthrough for protein sequencing.
NATURE COMMUNICATIONS
(2023)
Article
Cell Biology
Zu-Guo Zheng, Yin-Yue Xu, Wen-Ping Liu, Yang Zhang, Chong Zhang, Han-Ling Liu, Xiao-Yu Zhang, Run-Zhou Liu, Yi-Ping Zhang, Meng-Ying Shi, Hua Yang, Ping Li
Summary: Phytochemical atractylenolide II (AT II) has been found to ameliorate obesity-related lipid metabolism disorders by reducing hepatic sn-1,2-DAG levels and deactivating PKCε activity. It acts on a novel drug-binding pocket in DGKQ to regulate its kinase activity, and also activates the DGKQ-AMPK-PGC1a-UCP-1 signaling pathway to promote weight loss in adipose tissue.
Article
Cell Biology
Shaoqing Zhang, Xiuli Gong, Yiye Zhou, Qingwen Ma, Qin Cai, Guanheng Yang, Xinbing Guo, Yanwen Chen, Miao Xu, Yiwen Zhu, Yitao Zeng, Fanyi Zeng
Summary: This study investigates the role of maternal transcripts in early embryonic development by comparing transcriptomes from oocytes of high- or low-competence mouse strains. The results suggest that the transcription level of maternal messages, such as Prkce, in mature oocytes is associated with different 2-cell rates in select inbred mouse strains. Prkce transcript levels could serve as a potential biomarker to characterize high-quality mature oocytes.
CELL PROLIFERATION
(2022)
Article
Pharmacology & Pharmacy
Supachoke Mangmool, Ei Thet Htar Kyaw, Narawat Nuamnaichati, Sudhir Pandey, Warisara Parichatikanond
Summary: This study demonstrates that stimulation of A(1)Rs can attenuate oxidative stress-induced damage in heart cells, upregulate synthesis of antioxidant enzymes and antiapoptotic proteins, and exert cardioprotective effects through G beta gamma subunit-mediated A(1)R signaling pathway as well as PI3K/Akt and ERK1/2-dependent pathways.
TOXICOLOGY AND APPLIED PHARMACOLOGY
(2022)
Article
Orthopedics
Y. Cui, M. Z. Miao, M. Wang, Q. P. Su, K. Qiu, L. Arbeeva, S. Chubinskaya, B. O. Diekman, R. F. Loeser
Summary: This study aimed to investigate the effect of YAP phosphorylation and nuclear translocation on the chondrocyte response to osteoarthritis (OA) relevant stimuli. Normal human articular chondrocytes were treated with various stimuli, and YAP function was assessed. The results showed that YAP nuclear translocation was regulated by phosphorylation in response to anabolic and catabolic stimuli. In OA cartilage, YAP immunostaining was increased but sequestered in the cytosol in areas with more severe damage.
OSTEOARTHRITIS AND CARTILAGE
(2023)
Article
Pharmacology & Pharmacy
Soma Noguchi, Taketoshi Kajimoto, Takuya Kumamoto, Masashi Shingai, Soshi Narasaki, Tomoaki Urabe, Serika Imamura, Kana Harada, Izumi Hide, Sigeru Tanaka, Yuhki Yanase, Shun-Ichi Nakamura, Yasuo M. Tsutsumi, Norio Sakai
Summary: This study investigated the action mechanisms of propofol-induced protein kinase C (PKC) translocation. The results showed that propofol can translocate different subtypes of PKC to various cellular structures and alter the permeability of the nuclear envelope to induce nuclear translocation of PKCs and other proteins.
FRONTIERS IN PHARMACOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Lorena Agudo-Ibanez, Marta Morante, Lucia Garcia-Gutierrez, Andrea Quintanilla, Javier Rodriguez, Alberto Munoz, Javier Leon, Piero Crespo
Summary: The transcription factor MYC is regulated by the kinase activity of ERK2 and plays a role in cell proliferation, transformation, and survival. ERK2 was found to directly regulate MYC transcription by binding to the MYC promoter. ERK2 increased MYC expression and protein abundance in the nucleus, independent of its kinase activity, through interactions with CDK9. These findings demonstrate a kinase-independent role for ERK2 in promoting MYC expression by anchoring CDK9 to the MYC promoter.
Article
Biochemistry & Molecular Biology
Juying Jiao, Linjie Ruan, Chien-shan Cheng, Fengjiao Wang, Peiwen Yang, Zhen Chen
Summary: In this study, aberrantly expressed kinases in pancreatic cancer (PC) were identified, and the role of RIPK2 kinase was validated in promoting cancer cell proliferation, migration, and invasion. RIPK2 knockout inhibited tumor growth and metastasis in PC, and also affected autophagosome formation, ROS production, and cell apoptosis. Additionally, the interaction between oncogenic kinase PRKCI and RIPK2 was found to enhance the downstream phosphorylation of NF-κB, JNK, and ERK.
MOLECULAR MEDICINE
(2023)
Article
Multidisciplinary Sciences
Jia Song, Wei Zhao, Xin Zhang, Wenyu Tian, Xuyang Zhao, Liang Ma, Yongtong Cao, Yuxin Yin, Xuehui Zhang, Xuliang Deng, Dan Lu
Summary: A study has identified frameshift germline mutations of RIG-I in colon cancer patients, which may be associated with increased susceptibility to colon cancer. The study provides insights into the action of mutant RIG-I and proposes a potential strategy for the treatment of colon cancer.
NATURE COMMUNICATIONS
(2022)
Article
Reproductive Biology
Khushbukhat Khan, Sameen Zafar, Yasmin Badshah, Naeem Mahmood Ashraf, Mehak Rafiq, Lubna Danish, Maria Shabbir, Janeen H. Trembley, Tayyaba Afsar, Ali Almajwal, Suhail Razak
Summary: This study used bioinformatics approaches to predict the structure and localization of TPD52 and analyzed its molecular interactions. Real-time PCR-based expression analysis was performed to evaluate its co-expression with other oncogenic pathway proteins in ovarian cancer.
JOURNAL OF OVARIAN RESEARCH
(2023)
Article
Plant Sciences
Hongbo Li, Juan Wei, Yanglan Liao, Xiaoling Cheng, Shuhong Yang, Xiaohong Zhuang, Zhonghui Zhang, Wenjin Shen, Caiji Gao
Summary: This study reveals a previously unidentified function of plant-specific casein kinase I members in attenuating ABA signaling by regulating the phosphorylation and nuclear accumulation of FREE1.
PLANT CELL AND ENVIRONMENT
(2022)
Article
Medicine, Research & Experimental
Bo Zhao, Guang-Ping Li, Jian-Jun Peng, Li-Hui Ren, Li-Cheng Lei, Hui-Ming Ye, Zuo-Yan Wang, Sheng Zhao
Summary: Schizandrin B exerts cardioprotection against H/R injury in H9c2 cells through activation of the AMPK/Nrf2 pathway, demonstrating antioxidant and anti-inflammatory activities.
EXPERIMENTAL AND THERAPEUTIC MEDICINE
(2021)