Article
Cell Biology
Nan Tang, Kai Zhu, Cheng Jiang, Zhiyong Xiong, Qiangping Wang, Junjun Li, Weiming Xu
Summary: This study revealed the important role of RNF7 in the development of glioma. It was found that RNF7 promotes cell proliferation and inhibits apoptosis through activating the PI3K/AKT signaling pathway. Targeting the RNF7/PI3K/AKT axis may provide a new perspective on the prevention or treatment of glioma.
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
(2023)
Article
Multidisciplinary Sciences
Feng Zhi, Bowen Li, Chuanxia Zhang, Fan Xia, Rong Wang, Weihong Xie, Sihui Cai, Dawei Zhang, Ren Kong, Yiqiao Hu, Yilin Yang, Ya Peng, Jun Cui
Summary: NLRP6 enhances the PI3K/AKT pathway by binding and degrading p85 alpha, promoting tumorigenesis. The interaction between NLRP6 and p85 alpha offers a potential therapeutic target for tumor treatment.
NATURE COMMUNICATIONS
(2023)
Article
Oncology
Honglan Qu, Gao-wa Hasen, Yanyan Hou, Mengwei Ren, Jun Li, Baoshong Jing, YanDan Du
Summary: TRIM37 is upregulated in T-cell acute lymphoblastic leukemia (T-ALL), and it promotes the proliferation and reduces the sensitivity of T-ALL cells to bortezomib treatment by enhancing PTEN degradation and activating PI3K/AKT signaling pathway. These findings suggest that TRIM37 could be a potential therapeutic target for T-ALL.
FRONTIERS IN ONCOLOGY
(2023)
Article
Oncology
Liliana Ordonez, Giusy Tornillo, Howard Kendrick, Trevor Hay, Matthew John Smalley
Summary: The interaction between NOTCH and PI3K/AKT signaling pathways determines the development and type of mammary tumors in mice.
Review
Biochemistry & Molecular Biology
Sushmaa Chandralekha Selvakumar, K. Auxzilia Preethi, Durairaj Sekar
Summary: Cancer is a global threat and current therapeutics lack specificity and have side effects. Researchers have been studying the PTEN/PI3K/AKT pathway and microRNAs as potential targets for novel cancer therapeutics. This review focuses on the role of miRNAs in regulating the PTEN/PI3K/AKT axis in various cancers.
BIOCHIMICA ET BIOPHYSICA ACTA-REVIEWS ON CANCER
(2023)
Article
Biotechnology & Applied Microbiology
Xuehan Bi, Xiao Lv, Dajiang Liu, Hongtao Guo, Guang Yao, Lijuan Wang, Xiaolei Liang, Yongxiu Yang
Summary: METTL3 promotes the maturation of miR-126-5p through m6A modification, which in turn targets PTEN to activate the PI3K/Akt/mTOR pathway in ovarian cancer progression. This study provides potential targets for future ovarian cancer treatment and reveals tumorigenic mechanisms regulated by m6A modification.
CANCER GENE THERAPY
(2021)
Article
Cell Biology
Yan Sun, Huan Zhang, Junpeng Meng, Feng Guo, Dianyun Ren, Heshui Wu, Xin Jin
Summary: This study demonstrates that aberrant PCSK9 upregulation promotes cell proliferation and sorafenib resistance in HCC by inducing AKT activation. Additionally, competitive inhibition of PCSK9 palmitoylation enhances the antitumor effects of sorafenib in HCC.
Article
Cell Biology
Shusheng Wu, Lulu Cao, Lihong Ke, Ying Yan, Huiqin Luo, Xiaoxiu Hu, Jiayu Niu, Huimin Li, Huijun Xu, Wenju Chen, Yueyin Pan, Yifu He
Summary: The study revealed that CENPK is overexpressed in gastric cancer and may promote gastric carcinogenesis through the PTEN-PI3K-AKT signaling pathway, indicating it as a potential target for cancer therapeutics in GC.
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
(2021)
Article
Medicine, Research & Experimental
Wei Wang, Ruiying Sun, Lizhong Zeng, Yang Chen, Na Zhang, Shiguang Cao, Shanshan Deng, Xia Meng, Shuanying Yang
Summary: The study revealed that GALNT2 is highly expressed in LUAD and is associated with poor prognosis. GALNT2 promotes cancer cell proliferation and migration by activating the Notch/Hes1-PTEN-PI3K/Akt signaling axis, suggesting a potential new therapeutic target for LUAD.
Article
Environmental Sciences
Yiming Zhang, Zhaoyi Liu, Jing Lan, Jinliang Wang, Zhiqiang Shen, Guangliang Shi, Shu Li
Summary: Cadmium accumulation in pigs can induce apoptosis in thymus cells and activate oxidative stress pathways, leading to oxidative damage to immune tissues. Additionally, exposure to cadmium can affect antioxidant indices and alter the levels of related genes in the PTEN/PI3K/AKT pathway, ultimately impacting cell survival.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2021)
Article
Medicine, General & Internal
Dongliang Liu, Xiulan Ma
Summary: This study investigated the regulatory effect of miR-508-3p in middle ear cholesteatoma, revealing that miR-508-3p overexpression can promote cell proliferation, while PTEN can reverse its effect on cell proliferation. The findings suggest a potential regulatory axis involving hsa_circ_0000007/miR-508-3p/ PTEN/ PI3K/Akt in the development of cholesteatoma.
INTERNATIONAL JOURNAL OF MEDICAL SCIENCES
(2021)
Article
Environmental Sciences
Shengchen Wang, Hao Wu, Xu Shi, Yue Wang, Shiwen Xu
Summary: The production of plastics worldwide has played a crucial role in advancing modern society, but the increasing accumulation of plastics waste in oceans, soils, and other areas has become a major environmental and animal health concern. Our understanding of the toxicological effects of plastics, particularly microplastics (MPs), is still limited. This study revealed a hepatotoxicity phenomenon dominated by polystyrene (PS) MPs in mouse models and cellular assays. It was found that the level of liver cell injury caused by PS-MPs was negatively correlated with the size of the particles, with small-sized particles inducing necroptosis and large-sized particles inducing apoptosis. Inhibition of autophagy alleviated PS-MPs-induced cell death and altered the form of death injury to some extent. This study opens up new avenues for investigating the biological and toxicological effects of MPs, and provides valuable insights for preventing and mitigating the health hazards associated with MPs.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Oncology
Xi Yu, Shenglan Li, Mingrui Pang, Yang Du, Tao Xu, Tao Bai, Kang Yang, Juncheng Hu, Shaoming Zhu, Lei Wang, Xiuheng Liu
Summary: This study revealed that downregulation of TSPAN7 in bladder cancer is associated with tumor progression and poor prognosis, and demonstrated the inhibitory effects of TSPAN7 on cancer cell proliferation and migration through multiple experiments. TSPAN7 inhibits bladder cancer cell growth by activating Bax, cleaved caspase-3, and PTEN pathways.
FRONTIERS IN ONCOLOGY
(2021)
Article
Environmental Sciences
Hongmin Lu, Kai Yin, Heng Su, Dongxu Wang, Yue Zhang, Lulu Hou, Jun Bo Li, Yu Wang, Mingwei Xing
Summary: This study found that polystyrene microplastics (PS-MPs) can cause lung injury in broiler chickens, leading to oxidative stress through inhibition of the PTEN/PI3K/AKT pathway and induction of autophagy and cell apoptosis. These results are of great significance to the toxicological study of PS-MPs and the protection of the ecosystem.
ENVIRONMENTAL TOXICOLOGY
(2023)
Article
Oncology
Haowei Wang, Yujia Chen, Qinzi Yuan, Lixia Chen, Peiling Dai, Xuenong Li
Summary: This study found that HRK is lowly expressed in colorectal cancer tissues. For the first time, it was shown that HRK promotes apoptosis and inhibits proliferation of colorectal cancer cells by inhibiting the PI3K/AKT/mTOR signaling pathway. HRK represents a potential target for the treatment of colorectal cancer.
FRONTIERS IN ONCOLOGY
(2022)
Article
Immunology
Matteo Stravalaci, Isabel Pagani, Elvezia Maria Paraboschi, Mattia Pedotti, Andrea Doni, Francesco Scavello, Sarah N. Mapelli, Marina Sironi, Chiara Perucchini, Luca Varani, Milos Matkovic, Andrea Cavalli, Daniela Cesana, Pierangela Gallina, Nicoletta Pedemonte, Valeria Capurro, Nicola Clementi, Nicasio Mancini, Pietro Invernizzi, Rafael Bayarri-Olmos, Peter Garred, Rino Rappuoli, Stefano Duga, Barbara Bottazzi, Mariagrazia Uguccioni, Rosanna Asselta, Elisa Vicenzi, Alberto Mantovani, Cecilia Garlanda
Summary: This study demonstrates that selected human humoral fluid-phase pattern recognition molecules can interact with SARS-CoV-2 in COVID-19, including PTX3 and MBL, exerting antiviral activity and being associated with disease severity. These findings have important implications for resistance and pathogenesis of COVID-19.
Editorial Material
Immunology
Rosanna Asselta, Elvezia Maria Paraboschi, Matteo Stravalaci, Pietro Invernizzi, Paolo Bonfanti, Andrea Biondi, Isabel Pagani, Mattia Pedotti, Andrea Doni, Francesco Scavello, Sarah N. Mapelli, Marina Sironi, Chiara Perucchini, Luca Varani, Milos Matkovic, Andrea Cavalli, Daniela Cesana, Pierangela Gallina, Nicoletta Pedemonte, Valeria Capurro, Nicola Clementi, Nicasio Mancini, Rafael Bayarri-Olmos, Peter Garred, Rino Rappuoli, Stefano Duga, Barbara Bottazzi, Mariagrazia Uguccioni, Elisa Vicenzi, Alberto Mantovani, Cecilia Garlanda
Article
Multidisciplinary Sciences
L. Del Core, D. Cesana, P. Gallina, Y. N. Serina Secanechia, L. Rudilosso, E. Montini, E. C. Wit, A. Calabria, M. A. Grzegorczyk
Summary: The study developed an advanced method to evaluate the clonal diversity of the hematopoietic system in gene therapy. By analyzing vector integration sites, the number of clones in the blood can be determined, providing a normalized entropy index.
SCIENTIFIC REPORTS
(2022)
Article
Multidisciplinary Sciences
Sebastian Wagner, Christoph Baldow, Andrea Calabria, Laura Rudilosso, Pierangela Gallina, Eugenio Montini, Daniela Cesana, Ingmar Glauche
Summary: High transduction rates of viral vectors ensure good gene delivery; however, multiple integration events can occur in the same cell. Here the authors use correlations between repeated measurements of integration site abundances to estimate their mutual similarity and identify clusters of co-occurring sites.
NATURE COMMUNICATIONS
(2022)
Article
Cell & Tissue Engineering
Samuele Ferrari, Aurelien Jacob, Daniela Cesana, Marianne Laugel, Stefano Beretta, Angelica Varesi, Giulia Unali, Anastasia Conti, Daniele Canarutto, Luisa Albano, Andrea Calabria, Valentina Vavassori, Carlo Cipriani, Maria Carmina Castiello, Simona Esposito, Chiara Brombin, Federica Cugnata, Oumeya Adjali, Eduard Ayuso, Ivan Merelli, Anna Villa, Raffaella Di Micco, Anna Kajaste-Rudnitski, Eugenio Montini, Magalie Penaud-Budloo, Luigi Naldini
Summary: This study reveals the unexpected load and persistence of AAV genomes and their fragments in gene editing using viral transduction. The accumulation of viral DNA triggers a sustained DNA damage response, leading to frequent integration in cells. Using an integrase-defective lentiviral vector can mitigate the adverse effects and improve gene editing efficiency.
Article
Biochemical Research Methods
Giulia Pais, Giulio Spinozzi, Daniela Cesana, Fabrizio Benedicenti, Alessandra Albertini, Maria Ester Bernardo, Bernhard Gentner, Eugenio Montini, Andrea Calabria
Summary: Longitudinal clonal tracking studies in gene therapy applications require comprehensive and high-throughput analysis tools. ISAnalytics is a new R package that allows users to assess safety and efficacy of treatments and analyze hematopoietic stem cell biology using vector integration sites as markers. It provides efficient data structures, reproducibility, and analytical control, making it suitable for various clonal tracking applications.
BRIEFINGS IN BIOINFORMATICS
(2022)
Article
Hematology
Andrea Calabria, Carlo Cipriani, Giulio Spinozzi, Laura Rudilosso, Simona Esposito, Fabrizio Benedicenti, Alessandra Albertini, Marie Pouzolles, Mirko Luoni, Serena Giannelli, Vania Broccoli, Mickael Guilbaud, Oumeya Adjali, Naomi Taylor, Valerie S. Zimmermann, Eugenio Montini, Daniela Cesana
Summary: Adeno-associated virus (AAV) vectors have been successfully used in gene therapy for genetic disorders. AAV can integrate into the host cell genome, and our study found that in T cells, AAV integrations mainly occur within the T-cell receptor genes. This finding suggests new possibilities for long-term AAV-mediated gene transfer in dividing cells.
Meeting Abstract
Biotechnology & Applied Microbiology
Andrea Calabria, Giulio Spinozzi, Daniela Cesana, Fabrizio Benedicenti, Giulia Pais, Serena Scala, Maria Rosa Lidonnici, Samantha Scaramuzza, Sarah Marktel, Bernhard Rudolf Gentner, Fabio Ciceri, Luigi M. Naldini, Giuliana Ferrari, Alessandro Aiuti, Eugenio Montini
Meeting Abstract
Hematology
Andrea Calabria, Giulio Spinozzi, Daniela Cesana, Fabrizio Benedicenti, Giulia Pais, Serena Scala, Maria Rosa Lidonnici, Samantha Scaramuzza, Alessandra Albertini, Simona Esposito, Fabiola De Mattia, Daniele Canarutto, Francesca Tucci, Maryam Omrani, Francesca Dionisio, Stefania Giannelli, Sarah Marktel, Valeria Calbi, Francesca Ferrua, Bernhard Gentner, Fabio Ciceri, Luigi Naldini, Giuliana Ferrari, Alessandro Aiuti, Eugenio Montini
Meeting Abstract
Hematology
Ingmar Glauche, Sebastian Wagner, Christoph Baldow, Andrea Calabria, Laura Rudilosso, Pierangela Gallina, Eugenio Montini, Daniela Cesana
EXPERIMENTAL HEMATOLOGY
(2022)
Meeting Abstract
Biotechnology & Applied Microbiology
Fabrizio Benedicenti, Andrea Calabria, Daniela Cesana, Alessandra Albertini, Simona Esposito, Giulia Pais, Erika Tenderini, Giulio Spinozzi, Victor Neduva, Ashkenaz Richard, Martijn H. Brugman, David J. Dow, Magnani F. Chiara, Maximilian Witzel, Christoph Klein, Marie Pouzolles, Naomi Taylor, Valerie Zimmermann, Andrea Biondi, Steven J. Howe, Luigi Naldini, Alessandro Aiuti, Eugenio Montini
Meeting Abstract
Biotechnology & Applied Microbiology
Andrea Calabria, Carlo Cipriani, Giulio Spinozzi, Simona Esposito, Fabrizio Benedicenti, Alessandra Albertini, Samuele Ferrari, Aurelien Jacob, Maria Carminia Castiello, Marie Pouzolles, Naomi Taylor, Valerie S. Zimmermann, Anna Villa, Luigi Naldini, Eugenio Montini, Daniela Cesana
Meeting Abstract
Biotechnology & Applied Microbiology
Andrea Calabria, Giulio Spinozzi, Fabrizio Benedicenti, Daniela Cesana, Giulia Pais, Serena Scala, Samantha Scaramuzza, Maria Rosa Lidonnici, Alessandra Albertini, Simona Esposito, Valeria Calbi, Francesca Fumagalli, Francesca Ferrua, Sara Marktel, Luigi Naldini, Giuliana Ferrari, Alessandro Aiuti, Eugenio Montini
Meeting Abstract
Biotechnology & Applied Microbiology
Cristina Colleoni, Daniela Cesana, Pierangela Gallina, Laura Rudilosso, Eugenio Montini
Meeting Abstract
Biotechnology & Applied Microbiology
Aurelien Jacob, Samuele Ferrari, Daniela Cesana, Marianne Laugel, Stefano Beretta, Angelica Varesi, Daniele Canarutto, Luisa Albano, Anastasia Conti, Ivan Merelli, Raffaella Di Micco, Eugenio Montini, Magalie Penaud-Budloo, Luigi Naldini