Article
Biochemistry & Molecular Biology
Maria Ovejero-Sanchez, Gloria Asensio-Juarez, Myriam Gonzalez, Pilar Puebla, Miguel Vicente-Manzanares, Rafael Pelaez, Rogelio Gonzalez-Sarmiento, Ana Belen Herrero
Summary: This study synthesized and characterized a new microtubule-destabilizing agent, PILA9, which showed strong cytotoxicity against OC cells. Additionally, the combination of microtubule-destabilizing agents with Panobinostat synergistically enhanced cytotoxicity, potentially through inducing alpha-tubulin acetylation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Xiaoyu Song, Fengrui Yang, Xu Liu, Peng Xia, Wu Yin, Zhikai Wang, Yong Wang, Xiao Yuan, Zhen Dou, Kai Jiang, Mingming Ma, Bing Hu, Rui Zhang, Chao Xu, Zhiyong Zhang, Ke Ruan, Ruijun Tian, Lin Li, Tao Liu, Donald L. Hill, Jianye Zang, Xing Liu, Jinsong Li, Jinke Cheng, Xuebiao Yao
Summary: This study identified a new mitosis-specific crotonylation-regulated astral microtubule-EB1-NuMA interaction that plays a crucial role in accurate spindle positioning. Real-time imaging of chromosome movements in HeLa cells expressing genetically encoded crotonylated EB1 demonstrated the importance of crotonylation dynamics for precise control of spindle orientation during metaphase-anaphase transition. These findings highlight a general signaling cascade that integrates protein crotonylation with accurate spindle positioning for chromosome stability in mitosis.
NATURE CHEMICAL BIOLOGY
(2021)
Article
Cell Biology
Tobias Kletter, Abin Biswas, Simone Reber
Summary: The article discusses the complexity of spindle construction in eukaryotic cells, as well as the dynamic interactions between spindles and the cytoplasm, emphasizing the importance of understanding microtubules and molecular collective behavior and their connection to the entire cellular environment.
CURRENT OPINION IN CELL BIOLOGY
(2022)
Article
Cell Biology
Danilo Lopes, Alexandre L. Seabra, Bernardo Orr, Helder Maiato
Summary: This study identifies high alpha-tubulin acetylation as a potential biomarker for cancer cell response to taxol and uncovers a mechanistic link between alpha-tubulin detyrosination and the suppression of MCAK activity in taxol-induced cytotoxicity.
JOURNAL OF CELL BIOLOGY
(2022)
Review
Biochemistry & Molecular Biology
John M. Ryniawec, Gregory C. Rogers
Summary: Centrosome instability is closely associated with diseases such as cancer, highlighting the importance of understanding the wide range of centrosome functions for inspiring new discoveries.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2021)
Article
Cell Biology
Emmanuel T. Nsamba, Abesh Bera, Michael Costanzo, Charles Boone, Mohan L. Gupta
Summary: Different α-tubulin isotypes play distinct roles in recruiting spindle positioning mechanisms, optimizing spindle positioning in cells. These findings provide novel mechanistic insights into the function of tubulin isotypes in diverse cellular processes.
JOURNAL OF CELL BIOLOGY
(2021)
Article
Cell Biology
Jose Martinez-Hernandez, Julie Parato, Aditi Sharma, Jean-Marc Soleilhac, Xiaoyi Qu, Ellen Tein, Andrew Sproul, Annie Andrieux, Yves Goldberg, Marie-Jo Moutin, Francesca Bartolini, Leticia Peris
Summary: This study found an accumulation of tubulin acetylation in post-mortem brain tissues from Alzheimer's disease patients and human neurons with the Alzheimer's familial APP-V717I mutation. Impaired tubulin re-tyrosination may contribute to this acetylation accumulation.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Grace Y. Liu, Shiau-Chi Chen, Gang-Hui Lee, Kritika Shaiv, Pin-Yu Chen, Hsuan Cheng, Shi-Rong Hong, Wen-Ting Yang, Shih-Han Huang, Ya-Chu Chang, Hsien-Chu Wang, Ching-Lin Kao, Pin-Chiao Sun, Ming-Hong Chao, Yian-Ying Lee, Ming-Jer Tang, Yu-Chun Lin
Summary: Microtubules, a crucial cellular structure, have been studied using chemo and optogenetics methods to disassemble specific types of microtubules. The results provide insights into their roles in cellular trafficking, organelle reorganization, and cell stiffness.
Review
Neurosciences
Graziella Cappelletti, Alessandra Maria Calogero, Chiara Rolando
Summary: This study discusses the importance of microtubule acetylation in controlling neuronal and glial function, as well as its role in neurodegenerative disorders. Research has found that microtubule acetylation is a key modification that controls intracellular transport and stability, potentially serving as a novel therapeutic intervention for diseases like Parkinson's.
NEUROSCIENCE LETTERS
(2021)
Article
Cell Biology
Nader Rahimi, Rachel X. Y. Ho, Kevin Brown Chandler, Kyle Oliver Corcino De la Cena, Razie Amraei, Ashley J. Mitchel, Nels Engblom, Catherine E. Costello
Summary: TMIGD1 interacts with ERM family proteins moesin and ezrin, regulating their cellular localization and impacting cell morphology, migration, and mitotic spindle organization. The findings provide a molecular framework for understanding the complex functional interplay between TMIGD1 and ERM family proteins in the regulation of cell adhesion and mitotic spindle assembly, with implications in physiological and pathological processes such as cancer progression.
JOURNAL OF BIOMEDICAL SCIENCE
(2021)
Review
Cell Biology
Alexandra P. Navarro, Iain M. Cheeseman
Summary: Faithful chromosome segregation in metazoans requires the establishment of a metaphase plate where all chromosomes are attached to mitotic spindle microtubules and aligned at the cell equator. This process relies on the precise coordination between multiple mechanisms that start upon nuclear envelope breakdown and culminate with chromosome congression to the spindle equator during prometaphase. Error correction mechanisms are in place to sense and correct erroneous kinetochore-microtubule attachments before anaphase.
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2021)
Article
Biology
Thomas S. McAlear, Susanne Bechstedt
Summary: This study reveals that CKAP2 is the most potent microtubule growth factor, as it strongly affects nucleation and growth rates of microtubules, and suppresses catastrophes. These findings are crucial for understanding the important role of CKAP2 in cell division.
Article
Biochemistry & Molecular Biology
Anne Nehlig, Cynthia Seiler, Yulia Steblyanko, Florent Dingli, Guillaume Arras, Damarys Loew, Julie Welburn, Claude Prigent, Marin Barisic, Clara Nahmias
Summary: Depletion of microtubule-associated protein ATIP3 reduces metaphase spindle length by interacting with Kif2A and its partner Dda3 in an Aurora kinase A-dependent manner. The absence of ATIP3 leads to the accumulation of Kif2A and Dda3 at spindle poles, affecting poleward microtubule flux and spindle shortening. ATIP3 maintains Aurora A activity on the poles to control Kif2A targeting and spindle size, highlighting their mutual regulation in controlling spindle length.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2021)
Article
Biology
Mihaela Jagric, Patrik Risteski, Jelena Martincic, Ana Milas, Iva M. Tolic
Summary: The study revealed that bridging fibers play a crucial role in chromosome alignment by promoting alignment through length-dependent forces. It was found that PRC1-mediated crosslinking of bridging microtubules and recruitment of kinesins to the bridging fiber are essential mechanisms for chromosome alignment.
Article
Oncology
Girish Rajendraprasad, Susana Eibes, Claudia Guasch Boldu, Marin Barisic
Summary: TH588 has been identified as a promising anti-cancer compound that stabilizes microtubules in mitotic cells, leading to mitotic arrest or cell death. Its anticancer properties largely depend on its ability to target microtubules.
Article
Cell Biology
Kengo Takahashi, Tomoaki Nagai, Shuhei Chiba, Keiko Nakayama, Kensaku Mizuno
JOURNAL OF CELL SCIENCE
(2018)
Article
Cell Biology
Hiroka Kashihara, Shuhei Chiba, Shin-ichiro Kanno, Koya Suzuki, Tomoki Yano, Sachiko Tsukita
Editorial Material
Cell Biology
Tomoaki Nagai
JOURNAL OF CELL SCIENCE
(2018)
Article
Cell Biology
Izumi Dateyama, Yoshihiro Sugihara, Shuhei Chiba, Reo Ota, Risa Nakagawa, Tetsuo Kobayashi, Hiroshi Itoh
JOURNAL OF CELL SCIENCE
(2019)
Editorial Material
Biochemistry & Molecular Biology
Siew Cheng Phua, Shuhei Chiba, Masako Suzuki, Emily Su, Elle C. Roberson, Ganesh V. Pusapati, Stephane Schurmans, Mitsutoshi Setou, Rajat Rohatgi, Jeremy F. Reiter, Koji Ikegami, Takanari Inoue
Article
Evolutionary Biology
Daiki X. Sato, Yuu Ishii, Tomoaki Nagai, Kazumasa Ohashi, Masakado Kawata
BMC EVOLUTIONARY BIOLOGY
(2019)
Article
Cell Biology
Yohei Katoh, Shuhei Chiba, Kazuhisa Nakayama
MOLECULAR BIOLOGY OF THE CELL
(2020)
Article
Cell Biology
Misato Okazaki, Takuya Kobayashi, Shuhei Chiba, Ryota Takei, Luxiaoxue Liang, Kazuhisa Nakayama, Yohei Katoh
MOLECULAR BIOLOGY OF THE CELL
(2020)
Article
Biochemistry & Molecular Biology
Takashi Yamazoe, Tomoaki Nagai, Shinya Umeda, Yuko Sugaya, Kensaku Mizuno
JOURNAL OF BIOLOGICAL CHEMISTRY
(2020)
Article
Biochemistry & Molecular Biology
Yamato Ishida, Takuya Kobayashi, Shuhei Chiba, Yohei Katoh, Kazuhisa Nakayama
Summary: Compound heterozygous mutations in IFT144 gene can lead to severe ciliary defects through a complex mechanism, where one allele can cause severe ciliary defects when combined with a hypomorphic allele.
HUMAN MOLECULAR GENETICS
(2021)
Article
Biology
Sayaka Fujisawa, Hantian Qiu, Shohei Nozaki, Shuhei Chiba, Yohei Katoh, Kazuhisa Nakayama
Summary: INPP5E is localized on the ciliary membrane via its prenyl moiety, with the ARL3 and ARL13B GTPases contributing to maintaining this localization through different mechanisms, with ARL13B mainly determining the ciliary localization of INPP5E through direct binding to it.
Article
Cell Biology
Shunya Hiyamizu, Hantian Qiu, Laura Vuolo, Nicola L. Stevenson, Caroline Shak, Kate J. Heesom, Yuki Hamada, Yuta Tsurumi, Shuhei Chiba, Yohei Katoh, David J. Stephens, Kazuhisa Nakayama
Summary: The dynein-2 complex is transported anterogradely within cilia to drive retrograde trafficking of the intraflagellar transport (IFT) machinery containing IFT-A and IFT-B complexes. There are multiple interactions between the dynein-2 and IFT-B subunits, including WDR60 and the DYNC2H1-DYNC2LI1 dimer from dynein-2, and IFT54 and IFT57 from IFT-B. These interactions play a crucial role in the connection between dynein-2 and IFT-B.
JOURNAL OF CELL SCIENCE
(2023)
Article
Multidisciplinary Sciences
Mingyue Jin, Sakiko Matsumoto, Takashi Ayaki, Hodaka Yamakado, Tomoyuki Taguchi, Natsuko Togawa, Ayumu Konno, Hirokazu Hirai, Hiroshi Nakajima, Shoji Komai, Ryuichi Ishida, Syuhei Chiba, Ryosuke Takahashi, Toshifumi Takao, Shinji Hirotsune
Summary: Parkinson's disease is a progressive neurodegenerative disorder, and research suggests that tyrosine hydroxylase can facilitate the formation of oligomers of alpha-synuclein through dopamine modification, potentially impacting disease pathogenesis and degeneration of dopaminergic neurons.
NATURE COMMUNICATIONS
(2022)
Article
Multidisciplinary Sciences
Koya Suzuki, Kosuke Yamaga, Reitaro Tokumasu, Tatsuya Katsuno, Hiroo Tanaka, Shuhei Chiba, Takeshi Yagi, Ichiro Katayama, Atsushi Tamura, Hiroyuki Murota, Sachiko Tsukita
Summary: Hair retention in infant mice is regulated by Claudin (CLDN)-1 and CLDN3, which maintain the appropriate layered architecture of hair follicles. Deficiencies in these proteins can lead to hair loss.
ANNALS OF THE NEW YORK ACADEMY OF SCIENCES
(2023)