Article
Biochemistry & Molecular Biology
Jingze Zang, Sandra Klemm, Charlotte Pain, Patrick Duckney, Zhiru Bao, Gina Stamm, Verena Kriechbaumer, Katharina Buerstenbinder, Patrick J. Hussey, Pengwei Wang
Summary: This study identified a crucial cytoskeletal-associated complex that is essential for maintaining and organizing cytoskeletal structure and ER morphology in plant cells.
Article
Multidisciplinary Sciences
Yun Xiang, Rui Lyu, Junjie Hu
Summary: Membrane curvature is generated by constructing a curved oligomeric scaffold formed by endoplasmic reticulum tubule-forming proteins. The mechanism of curvature generation remains elusive.
NATURE COMMUNICATIONS
(2023)
Article
Multidisciplinary Sciences
Yong Xue, Jiang-Guo Meng, Peng-Fei Jia, Zheng-Rong Zhang, Hong-Ju Li, Wei-Cai Yang
Summary: This study reports a mechanism for the sorting of cell surface receptors by three ER membrane localized proteins. The mechanism is crucial for the proper sorting of leucine-rich repeat receptor kinases (LRR-RKs) to the plasma membrane.
NATURE COMMUNICATIONS
(2022)
Article
Multidisciplinary Sciences
Marika K. Kucinska, Juliette Fedry, Carmela Galli, Diego Morone, Andrea Raimondi, Tatiana Solda, Friedrich Forster, Maurizio Molinari
Summary: This study reveals that expansion of the mammalian ER leads to the disassembly of LINC complexes and swelling of ONM. Upon resolution of ER stress, the physiologic distance between ONM and INM is restored through a catabolic pathway called micro-ONM-phagy.
NATURE COMMUNICATIONS
(2023)
Article
Cell Biology
Shanna L. Bowman, Linh Le, Yueyao Zhu, Dawn C. Harper, Anand Sitaram, Alexander C. Theos, Elena Sviderskaya, Dorothy C. Bennett, Graca Raposo-Benedetti, David J. Owen, Megan K. Dennis, Michael S. Marks
Summary: The vSNARE VAMP7 is sorted into transport carriers in complex with the tSNARE STX13, with sorting requiring recognition by either AP-3 delta or pallidin subunit of BLOC-1, but not both. This mechanism allows for redundant recognition of sorting determinants on a SNARE complex by an AP-3-BLOC-1 super-complex.
JOURNAL OF CELL BIOLOGY
(2021)
Article
Multidisciplinary Sciences
Qi Yang, Anju Kelkar, Anirudh Sriram, Ryoma Hombu, Thomas A. Hughes, Sriram Neelamegham
Summary: N-linked glycans on the SARS-CoV-2 Spike protein play a critical role in viral function and infectivity, making them potential drug targets for COVID-19.
Article
Cell Biology
Nan Liu, Hongyu Zhao, Yan G. Zhao, Junjie Hu, Hong Zhang
Summary: This study demonstrates the crucial role of ER-localized transmembrane proteins Atlastin 2 and 3 in recruiting and stabilizing ULK1 and ATG101 for autophagosome formation. In ATL2/3 KO cells, ULK1 and ATG101 targeting is severely impaired, affecting the initiation and speed of IM formation. ATL2/3 interact directly with ULK1 and ATG13, facilitating the recruitment/stabilization of ULK1 and ATG101 by ATG13, and also participate in forming ER-IM tethering complexes.
JOURNAL OF CELL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Yonis Bare, Tamas Matusek, Sophie Vriz, Maika S. Deffieu, Pascal P. Therond, Raphael Gaudin
Summary: This study demonstrates that newly-synthesised Sonic Hedgehog (SHH) is trafficked through the classical biosynthetic secretory pathway using TMED10 as a cargo receptor, and Rab6 vesicles for Golgi-to-cell surface trafficking. Depletion of TMED10 delays SHH loading onto ER exit sites (ERES) and impairs SHH release. Additionally, the homologue of TMED10, Baiser, is found to participate in Hedgehog (Hh) secretion and signalling in Drosophila wing imaginal disc model. Therefore, TMED10 plays a crucial role in SHH secretion and its interaction with other proteins affects embryonic development.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2023)
Article
Cell Biology
Yuki Ishiwata-Kimata, Quynh Giang Le, Yukio Kimata
Summary: This article investigates the synthesis pathways of phosphatidylcholine (PC) and its role in endoplasmic reticulum (ER) stress. The study found that the opi3 gene mutation can induce ER stress even when free choline is sufficiently supplied. Additionally, monomethylethanolamine produced through the Kennedy pathway may act as a molecule that induces ER stress.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Sascha Waidmann, Chloe Beziat, Jonathan Ferreira Da Silva Santos, Elena Feraru, Mugurel I. Feraru, Lin Sun, Seinab Noura, Yohann Boutte, Juergen Kleine-Vehn
Summary: This study reveals a mechanism that integrates multiple external cues into auxin-dependent growth programs in Arabidopsis thaliana. This mechanism involves the regulation of PIN-LIKES (PILS) protein abundance and turnover, which affects nuclear auxin signaling and growth rates, contributing to stress-induced growth adaptation in plants.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Review
Biochemistry & Molecular Biology
Martin Jung, Richard Zimmermann
Summary: Through quantitative mass spectrometry analysis, the redundancy in the components for the targeting and insertion of membrane proteins into the endoplasmic reticulum (ER) was studied. Different signal peptide sequences were found to affect the targeting and insertion of membrane proteins, and some auxiliary components were found to share similar client spectra with the Sec61 complex.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell Biology
Pawel Leznicki, Hayden O. Schneider, Jada Harvey, Wei Q. Shi, Stephen High
Summary: Membrane proteins destined for lipid droplets can be delivered to the endoplasmic reticulum either co- or post-translationally. The co-translational pathway relies on the ER membrane protein complex for membrane insertion, and may result in structural rearrangements.
JOURNAL OF CELL SCIENCE
(2022)
Review
Biochemistry & Molecular Biology
Sarah R. Barger, Lauren Penfield, Shirin Bahmanyar
Summary: The nuclear envelope serves as a protective barrier for the cell's genome, undergoing dynamic remodeling processes essential for cell growth and maintenance. Recent studies have shown that lipids produced in the endoplasmic reticulum are utilized to remodel nuclear membranes, with lipid species and lipid-protein interactions playing crucial roles in controlling nuclear envelope dynamics. Coordination of lipid biosynthesis is important for ensuring genome protection through nuclear envelope remodeling.
TRENDS IN BIOCHEMICAL SCIENCES
(2022)
Article
Cell Biology
Banafsheh Golchoubian, Andreas Brunner, Helena Bragulat-Teixidor, Annett Neuner, Busra A. Akarlar, Nurhan Ozlu, Anne-Lore Schlaitz
Summary: Nuclear pore complexes (NPCs) are channels within the nuclear envelope that mediate nucleocytoplasmic transport, forming through a coordination of protein complex assembly and membrane deformation. REEP4 plays an important role in NPC formation during mitosis, being recruited by the NPC biogenesis factor ELYS. Their cooperation may provide high-curvature ER membrane to the nascent NPC.
JOURNAL OF CELL BIOLOGY
(2021)
Article
Virology
Yingyi Duan, Xinhui Wang, Kehui Sun, Yuezhi Lin, Xuefeng Wang, Kewei Chen, Guangpu Yang, Xiaojun Wang, Cheng Du
Summary: This study found that SYNJ2BP can promote the production of lentiviral Env proteins by increasing the formation of MAMs and enhance the infectivity of the virus. Additionally, MAMs can provide ATP and calcium ions to the ER, reduce ER stress, and increase the production of Env.
JOURNAL OF VIROLOGY
(2022)
Article
Biochemistry & Molecular Biology
Anja Nylander, Pawel Leznicki, Karina Vidovic, Stephen High, Martin L. Olsson
BIOSCIENCE REPORTS
(2020)
Article
Biochemistry & Molecular Biology
Pawel Leznicki, Stephen High
Editorial Material
Biochemistry & Molecular Biology
Sarah O'Keefe, Stephen High
Article
Biochemistry & Molecular Biology
Maurice Steenhuis, Gregory M. Koningstein, Julia Oswald, Tillman Pick, Sarah O'Keefe, Hans-Georg Koch, Adolfo Cavalie, Roger C. Whitehead, Eileithyia Swanton, Stephen High, Joen Luirink
Summary: ES24 is an inhibitor that affects bacterial protein secretion and insertion by targeting the SecYEG translocon. This compound has a complex mode of action, potentially involving multiple targets, and exhibits antibacterial activity which requires activation by nitroreductases.
MOLECULAR MICROBIOLOGY
(2021)
Article
Cell Biology
Sarah O'Keefe, Peristera Roboti, Kwabena B. Duah, Guanghui Zong, Hayden Schneider, Wei Q. Shi, Stephen High
Summary: The study found that the small molecule inhibitor Ipom-F can effectively block the synthesis of SARS-CoV-2 infection proteins to reduce pathogenicity, suggesting a new strategy for antiviral drugs.
JOURNAL OF CELL SCIENCE
(2021)
Review
Biochemistry & Molecular Biology
Sarah O'Keefe, Martin R. Pool, Stephen High
Summary: The Sec61 complex in the ER plays a central role in protein translocation, recruiting accessory complexes and coordinating the co-translational insertion of proteins. Some transmembrane proteins preferentially utilize alternative pathways for insertion. Our understanding of post-insertion events has improved, with embedded chaperone complexes aiding in the assembly of multi-span proteins.
Article
Cell Biology
Pawel Leznicki, Hayden O. Schneider, Jada Harvey, Wei Q. Shi, Stephen High
Summary: Membrane proteins destined for lipid droplets can be delivered to the endoplasmic reticulum either co- or post-translationally. The co-translational pathway relies on the ER membrane protein complex for membrane insertion, and may result in structural rearrangements.
JOURNAL OF CELL SCIENCE
(2022)
Article
Multidisciplinary Sciences
Peristera Roboti, Sarah O'Keefe, Kwabena B. Duah, Wei Q. Shi, Stephen High
Summary: Ipomoeassin-F (Ipom-F) selectively inhibits protein entry into the ER lumen, affecting the in vitro translocation of certain secretory proteins and leading to incomplete folding of some proteins in the ER lumen. This is likely due to Ipom-F preventing the cell from replenishing its ER lumenal chaperones, resulting in an atypical unfolded protein response (UPR).
SCIENTIFIC REPORTS
(2021)
Article
Biology
Sarah O'Keefe, Guanghui Zong, Kwabena B. Duah, Lauren E. Andrews, Wei Q. Shi, Stephen High
Summary: The integration of type III single-pass transmembrane proteins targeted to the ER and the roles of the ER membrane complex, Sec61 complex and signal recognition particle receptor were investigated by O'Keefe et al. The authors proposed an alternative mechanism of insertion, reconciling the ability of type III transmembrane proteins to bypass an ipomoeassin-F-mediated blockade of membrane integration.
COMMUNICATIONS BIOLOGY
(2021)
Article
Cell Biology
Gabrielle Parkinson, Peristera Roboti, Ling Zhang, Sandra Taylor, Philip Woodman
Summary: HD-PTP collaborates with ESCRTs to sort endosomal cargo into MVB, and is crucial for endosomal maturation. Depletion of HD-PTP inhibits MVB sorting and causes cargo to be trapped in Rab5-rich endosomes.
JOURNAL OF CELL SCIENCE
(2021)
Article
Cell Biology
Peristera Roboti, Craig Lawless, Stephen High
Summary: The heterotrimeric BAG6 complex plays a role in coordinating the transport of newly synthesised membrane proteins and processing defective precursors. It also interacts with the mitochondrial antiviral-signalling protein MAVS and regulates its role in the cellular response to viral infection.
JOURNAL OF CELL SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Sarah O'Keefe, Pratiti Bhadra, Kwabena B. Duah, Guanghui Zong, Levise Tenay, Lauren Andrews, Hayden Schneider, Ashley Anderson, Zhijian Hu, Hazim S. Aljewari, Belinda S. Hall, Rachel E. Simmonds, Volkhard Helms, Stephen High, Wei Q. Shi
Summary: This study demonstrates that macrocyclic integrity is not necessary for the cytotoxic inhibition of Sec61-dependent protein translocation by Ipom-F. The researchers find that open-chain analogues can interact with multiple sites on the Sec61 alpha subunit. Additionally, they discover a stereochemically simplified analogue that is more potent and easier to synthesize.
Article
Cell Biology
Andrea Tirincsi, Sarah O'Keefe, Duy Nguyen, Mark Sicking, Johanna Dudek, Friedrich Forster, Martin Jung, Drazena Hadzibeganovic, Volkhard Helms, Stephen High, Richard Zimmermann, Sven Lang
Summary: Importing proteins into the endoplasmic reticulum is crucial for approximately 30% of the human proteome, involving precursor protein targeting and insertion or translocation across the ER membrane with the help of signals in precursor polypeptides. Little is known about the SRP-independent/SND pathway, and our study aimed to identify additional components and characterize the client spectrum of this pathway, finding that SND clients are predominantly membrane proteins.
Biographical-Item
Biochemistry & Molecular Biology
Ineke Braakman, Stephen High, Karl Kadler, Roberto Sitia, Kostas Tokatlidis, Philip Woodman