Imaging molecular dynamics in vivo – from cell biology to animal models

Actomyosin-Mediated Cellular Tension Drives Increased Tissue Stiffness and β-Catenin Activation to Induce Epidermal Hyperplasia and Tumor Growth

(2011) Michael S. Samuel et al.   CANCER CELL

Spatial Regulation of RhoA Activity during Pancreatic Cancer Cell Invasion Driven by Mutant p53

(2011) P. Timpson et al.   CANCER RESEARCH

Self-organizing optic-cup morphogenesis in three-dimensional culture

(2011) Mototsugu Eiraku et al.   NATURE

4-Methylumbelliferone inhibits tumour cell growth and the activation of stromal hyaluronan synthesis by melanoma cell-derived factors

(2010) M. Edward et al.   BRITISH JOURNAL OF DERMATOLOGY

Quantitative In vivo Imaging of the Effects of Inhibiting Integrin Signaling via Src and FAK on Cancer Cell Movement: Effects on E-cadherin Dynamics

(2010) M. Canel et al.   CANCER RESEARCH

Designing multifunctional quantum dots for bioimaging, detection, and drug delivery

(2010) Pavel Zrazhevskiy et al.   CHEMICAL SOCIETY REVIEWS

Quantitative understanding of cell signaling: the importance of membrane organization

(2010) Krishnan Radhakrishnan et al.   CURRENT OPINION IN BIOTECHNOLOGY

The Rho kinase Rock2b establishes anteroposterior asymmetry of the ciliated Kupffer's vesicle in zebrafish

(2010) G. Wang et al.   DEVELOPMENT

Differential Regulation of Protrusion and Polarity by PI(3)K during Neutrophil Motility in Live Zebrafish

(2010) Sa Kan Yoo et al.   DEVELOPMENTAL CELL

p21 loss blocks senescence following Apc loss and provokes tumourigenesis in the renal but not the intestinal epithelium

(2010) Alicia M. Cole et al.   EMBO Molecular Medicine

Optical imaging of tumor hypoxia dynamics

(2010) Gregory M. Palmer et al.   JOURNAL OF BIOMEDICAL OPTICS

A guide to super-resolution fluorescence microscopy

(2010) Lothar Schermelleh et al.   JOURNAL OF CELL BIOLOGY

Intravital imaging of DSS-induced cecal mucosal damage in GFP-transgenic mice using two-photon microscopy

(2010) Yuji Toiyama et al.   JOURNAL OF GASTROENTEROLOGY

Optimizing multiphoton fluorescence microscopy light collection from living tissue by noncontact total emission detection (epiTED)

(2010) C.A. COMBS et al.   JOURNAL OF MICROSCOPY

p53 mutation and loss have different effects on tumourigenesis in a novel mouse model of pleomorphic rhabdomyosarcoma

(2010) Brendan Doyle et al.   JOURNAL OF PATHOLOGY

Anisotropies in cortical tension reveal the physical basis of polarizing cortical flows

(2010) Mirjam Mayer et al.   NATURE

Light-mediated activation reveals a key role for Rac in collective guidance of cell movement in vivo

(2010) Xiaobo Wang et al.   NATURE CELL BIOLOGY

Reducing background fluorescence reveals adhesions in 3D matrices

(2010) Kristopher E. Kubow et al.   NATURE CELL BIOLOGY

Rapid blue-light–mediated induction of protein interactions in living cells

(2010) Matthew J Kennedy et al.   NATURE METHODS

β-Catenin activation synergizes with PTEN loss to cause bladder cancer formation

(2010) I Ahmad et al.   ONCOGENE

Ex vivo live imaging of melanoblast migration in embryonic mouse skin

(2010) Richard L. Mort et al.   Pigment Cell & Melanoma Research

In Vivo Imaging of HIF-Active Tumors by an Oxygen-Dependent Degradation Protein Probe with an Interchangeable Labeling System

(2010) Takahiro Kuchimaru et al.   PLoS One

A Novel Technique for the In Vivo Imaging of Autoimmune Diabetes Development in the Pancreas by Two-Photon Microscopy

(2010) Ken Coppieters et al.   PLoS One

Monomeric red fluorescent proteins with a large Stokes shift

(2010) K. D. Piatkevich et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Live imaging of Drosophila imaginal disc development

(2010) S. Aldaz et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Cell Lineage Reconstruction of Early Zebrafish Embryos Using Label-Free Nonlinear Microscopy

(2010) N. Olivier et al.   SCIENCE

Video-Rate Molecular Imaging in Vivo with Stimulated Raman Scattering

(2010) B. G. Saar et al.   SCIENCE

Intravascular Danger Signals Guide Neutrophils to Sites of Sterile Inflammation

(2010) B. McDonald et al.   SCIENCE

Wide-field fluorescence lifetime imaging of cancer

(2010) James McGinty et al.   Biomedical Optics Express

Stretching the timescale of intravital imaging in tumors

(2010) Bojana Gligorijevic et al.   Cell Adhesion & Migration

Quantitative real-time imaging of molecular dynamics during cancer cell invasion and metastasis in vivo

(2010) Paul Timpson et al.   Cell Adhesion & Migration

Cdc42-dependent leading edge coordination is essential for interstitial dendritic cell migration

(2009) T. Lammermann et al.   BLOOD

Real-time Study of E-Cadherin and Membrane Dynamics in Living Animals: Implications for Disease Modeling and Drug Development

(2009) A. Serrels et al.   CANCER RESEARCH

Adaptive optics for deeper imaging of biological samples

(2009) John M Girkin et al.   CURRENT OPINION IN BIOTECHNOLOGY

Infrared multiphoton microscopy: subcellular-resolved deep tissue imaging

(2009) Volker Andresen et al.   CURRENT OPINION IN BIOTECHNOLOGY

Fluorescence lifetime imaging microscopy of chemotherapy-induced apoptosis resistance in a syngenic mouse tumor model

(2009) M. Keese et al.   INTERNATIONAL JOURNAL OF CANCER

Spatiotemporal control of cell signalling using a light-switchable protein interaction

(2009) Anselm Levskaya et al.   NATURE

A genetically encoded photoactivatable Rac controls the motility of living cells

(2009) Yi I. Wu et al.   NATURE

Single Lgr5 stem cells build crypt-villus structures in vitro without a mesenchymal niche

(2009) Toshiro Sato et al.   NATURE

A role for Rho GTPases and cell–cell adhesion in single-cell motility in vivo

(2009) Elena Kardash et al.   NATURE CELL BIOLOGY

Localized and reversible TGFβ signalling switches breast cancer cells from cohesive to single cell motility

(2009) Silvia Giampieri et al.   NATURE CELL BIOLOGY

Three-dimensional microscopy of the tumor microenvironment in vivo using optical frequency domain imaging

(2009) Benjamin J Vakoc et al.   NATURE MEDICINE

Modular scanning FCS quantifies receptor-ligand interactions in living multicellular organisms

(2009) Jonas Ries et al.   NATURE METHODS

In vivo imaging of extracellular matrix remodeling by tumor-associated fibroblasts

(2009) Jean Y Perentes et al.   NATURE METHODS

Mutant p53 drives metastasis and overcomes growth arrest/senescence in pancreatic cancer

(2009) J. P. Morton et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Mammalian Expression of Infrared Fluorescent Proteins Engineered from a Bacterial Phytochrome

(2009) X. Shu et al.   SCIENCE

Inhibition of Hedgehog Signaling Enhances Delivery of Chemotherapy in a Mouse Model of Pancreatic Cancer

(2009) K. P. Olive et al.   SCIENCE

Collagen-based cell migration models in vitro and in vivo

(2009) Katarina Wolf et al.   SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY

The potential of optical proteomic technologies to individualize prognosis and guide rational treatment for cancer patients

(2009) Muireann T. Kelleher et al.   Targeted Oncology

Time-lapse Live Cell Imaging and Flow Analysis of Multidrug Resistance Reversal by Verapamil in Bladder Cancer Cell Lines

(2009) Jonathan M. Featherstone et al.   UROLOGY

Dendra2 Photoswitching through the Mammary Imaging Window

(2009) Bojana Gligorijevic et al.   Jove-Journal of Visualized Experiments

Fluorescence lifetime imaging distinguishes basal cell carcinoma from surrounding uninvolved skin

(2008) N.P. Galletly et al.   BRITISH JOURNAL OF DERMATOLOGY

Multiphoton microscopy and fluorescence lifetime imaging microscopy (FLIM) to monitor metastasis and the tumor microenvironment

(2008) Paolo P. Provenzano et al.   CLINICAL & EXPERIMENTAL METASTASIS

Visualizing and quantifying adhesive signals

(2008) Mohsen Sabouri-Ghomi et al.   CURRENT OPINION IN CELL BIOLOGY

Visualizing stromal cell dynamics in different tumor microenvironments by spinning disk confocal microscopy

(2008) M. Egeblad et al.   Disease Models & Mechanisms

Real-time imaging of the cellular interactions underlying tolerance, priming, and responses to infection

(2008) Paul Garside et al.   IMMUNOLOGICAL REVIEWS

A two-tiered mechanism for stabilization and immobilization of E-cadherin

(2008) Matthieu Cavey et al.   NATURE

Myelomonocytic cell recruitment causes fatal CNS vascular injury during acute viral meningitis

(2008) Jiyun V. Kim et al.   NATURE

Rapid leukocyte migration by integrin-independent flowing and squeezing

(2008) Tim Lämmermann et al.   NATURE

PDK1 regulates cancer cell motility by antagonising inhibition of ROCK1 by RhoE

(2008) Sophie Pinner et al.   NATURE CELL BIOLOGY

Detection of colonic dysplasia in vivo using a targeted heptapeptide and confocal microendoscopy

(2008) Pei-Lin Hsiung et al.   NATURE MEDICINE

Improving the photostability of bright monomeric orange and red fluorescent proteins

(2008) Nathan C Shaner et al.   NATURE METHODS

Intravital imaging of metastatic behavior through a mammary imaging window

(2008) Dmitriy Kedrin et al.   NATURE METHODS

Reconstruction of Zebrafish Early Embryonic Development by Scanned Light Sheet Microscopy

(2008) P. J. Keller et al.   SCIENCE

Image Correlation Spectroscopy of Multiphoton Images Correlates with Collagen Mechanical Properties

(2007) Christopher B. Raub et al.   BIOPHYSICAL JOURNAL