Review
Nanoscience & Nanotechnology
Yifan Da, Shihua Luo, Yang Tian
Summary: This Review focuses on the progress in the construction of sensors for in vivo analysis of neurotransmitters in the brain and summarizes current attempts to address key issues in the development of sensors with high selectivity, sensitivity, and stability. Several strategies for sensor construction are provided for recording chemical signal changes in the complex environment of the brain, combined with the latest advances in technologies and methods.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Multidisciplinary Sciences
Shigetaka Nishiguchi, Tadaomi Furuta, Takayuki Uchihashi
Summary: Classical cadherins are important in cell-cell adhesion. High-speed atomic force microscopy (HS-AFM) was used to observe full-length ectodomains of E-cadherin and discovered previously unreported dimeric structures, with about half of the cadherin dimers showing S-shaped conformations and greater dynamics than SS and X-like dimers. Mutational and molecular modeling analyses revealed that the S-shaped dimer had a different binding interface from SS and X-dimers. The formation of SS-dimer from S-shaped and X-like dimers was directly visualized.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Chemistry, Multidisciplinary
Silan Toy, Joern Dietz, Peter Naumann, Janina Trothe, Franziska Thomas, Ulf Diederichsen, Claudia Steinem, Andreas Janshoff
Summary: Cell adhesion molecules, such as E-cadherin, play a crucial role in various biological processes. Researchers synthesized E-cadherin mimetic peptides and attached them to solid-supported membranes on colloidal probes to identify this molecule on cell surfaces. Two different functionalization strategies were established, and binding studies showed successful attachment of the peptides to the membrane surface. The presence of E-cadherin on living cells was confirmed using colloidal probes functionalized with HAV-peptides.
CHEMISTRY-A EUROPEAN JOURNAL
(2023)
Article
Pharmacology & Pharmacy
Yuchen Zhang, Zhongwang Dou, Jean-Christophe Veilleux, Galen H. Shi, David S. Collins, Pavlos P. Vlachos, Sadegh Dabiri, Arezoo M. Ardekani
Summary: The collapse of cavitation bubbles induced by abrupt acceleration of the syringe in an autoinjector device can lead to protein aggregation. The phenomenon is more prominent for bubbles positioned closer to the bottom wall, growing to a larger maximum radius. The strain rate induced by the bubble collapse is not large enough to unfold the proteins.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2021)
Review
Biochemistry & Molecular Biology
Huichen Zhao, Huihui Hu, Beibei Chen, Weifeng Xu, Jing Zhao, Chen Huang, Yishu Xing, Huifang Lv, Caiyun Nie, Jianzheng Wang, Yunduan He, Sai-Qi Wang, Xiao-Bing Chen
Summary: Gastric cancer ranks fifth in global cancer incidence and third in cancer mortality. E-cadherin is crucial in tumorigenesis, tumor progression, metastasis, with soluble E-cadherin playing a role in promoting invasion and metastasis in gastric cancer.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2021)
Article
Multidisciplinary Sciences
Giovanna Grimaldi, Angela Filograna, Laura Schembri, Matteo Lo Monte, Rosaria Di Martino, Marinella Pirozzi, Daniela Spano, Andrea R. Beccari, Seetharaman Parashuraman, Alberto Luini, Carmen Valente, Daniela Corda
Summary: This study reveals that the mono-ADP-ribosylation of Golgin-97 by PARP12 selectively regulates the transport of specific basolateral proteins, contributing to the maintenance of cell polarity and cell-cell junctions. The enzymatic activity of PARP12 depends on the activation of protein kinase D, which is essential for its catalytic activity and involvement in the basolateral transport pathway.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Biochemistry & Molecular Biology
Man Lee Yuen, Ling Zhuang, Emma M. Rath, Takun Yu, Ben Johnson, Kadir Harun Sarun, Yiwei Wang, Steven Kao, Anthony Linton, Candice Julie Clarke, Brian C. McCaughan, Ken Takahashi, Kenneth Lee, Yuen Yee Cheng
Summary: Research on the role of E-cadherin and microRNA in the efficacy of FAK inhibitors in MPM revealed that MPM cells with high CDH1 mRNA levels exhibited resistance to the FAK inhibitor PND-1186. Additionally, PND-1186 induced cell cycle disruption by inducing the G2/M arrest of MPM cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Ariana Abawi, Xiaoyi Wang, Julien Bompard, Anna Berot, Valentina Andretto, Leslie Gudimard, Chloe Devillard, Emma Petiot, Benoit Joseph, Giovanna Lollo, Thierry Granjon, Agnes Girard-Egrot, Ofelia Maniti
Summary: Novel nanomedicines have been developed to deliver therapeutic molecules effectively, with lipid-based carriers like liposomes being one of the most advanced drug delivery systems. By modulating the liposome composition, selective uptake from tumor cells while sparing normal cells can be achieved, demonstrating the potential of fluid liposomes grafted with MMAE as an interesting drug carrier option.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Annett Petrich, Amit Koikkarah Aji, Valentin Dunsing, Salvatore Chiantia
Summary: Protein-protein interactions are crucial for cellular functions, and non-invasive techniques are used to evaluate these interactions in living cells for a better understanding of biological processes. Fluorescence fluctuation spectroscopy is a quantitative microscopy approach that characterizes molecular interactions at single cell resolution. Evaluating the photophysical behavior of fluorescent proteins is critical for selecting appropriate tags for live cell imaging applications.
Article
Multidisciplinary Sciences
Raudel Avila, Chenhang Li, Yeguang Xue, John A. Rogers, Yonggang Huang
Summary: Drug delivery systems with electrochemical actuation offer programmable volume/flowrate capabilities for localized delivery. A new analytical model considering microfluidic resistance and membrane stiffness provides scalable understanding of key variables influencing the delivery process, allowing faster system optimization. The results show relevance to emerging applications of programmable delivery in clinical studies within the neuroscience and biomedical communities.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Chemistry, Multidisciplinary
Ikuhiko Nakase, Moe Miyai, Kosuke Noguchi, Mamoru Tamura, Yasuyuki Yamamoto, Yushi Nishimura, Mika Omura, Kota Hayashi, Shiroh Futaki, Shiho Tokonami, Takuya Iida
Summary: In this study, we demonstrate the application of light-induced assembly for drug delivery system which enhances the accumulation and penetration of biofunctional molecules in cell membranes through superradiance-mediated photothermal convection.
Article
Pharmacology & Pharmacy
Christina Kovar, Lukas Kovar, Simeon Ruedesheim, Dominik Selzer, Boian Ganchev, Patrick Kroener, Svitlana Igel, Reinhold Kerb, Elke Schaeffeler, Thomas E. Muerdter, Matthias Schwab, Thorsten Lehr
Summary: This study developed a whole-body PBPK model to describe and predict the effects of drug-gene interactions and drug-drug interactions on the exposure of clomiphene and its metabolites. The model showed good performance in predicting the effects with clarithromycin and paroxetine, and quantitatively assessed the contributions of different CYP enzymes to the metabolic pathways of clomiphene and its metabolites.
Article
Pharmacology & Pharmacy
Helen Farrants, Alison G. Tebo
Summary: Fluorescent indicators and actuators are useful tools for observing and perturbing dynamic events in living animals. The emerging strategy of chemigenetics combines small molecule dyes with genetically encoded protein domains, providing many advantages.
CURRENT OPINION IN PHARMACOLOGY
(2022)
Article
Multidisciplinary Sciences
Elias Amselem, Bo Broadwater, Tora Haevermark, Magnus Johansson, Johan Elf
Summary: This study presents a 3D tracking method based on cross-entropy minimization and the true excitation point spread function, which enables sub-millisecond tracking of individual molecules in living cells. The method achieves high precision and time resolution in tracking beads and has been successfully applied to track a specific protein in living bacterial cells. However, it is still challenging to resolve state transitions based on diffusivity at this time scale.
NATURE COMMUNICATIONS
(2023)
Article
Engineering, Marine
Ling Zhu, Qin Chen, Hongqing Wang, Nan Wang, Kelin Hu, William Capurso, Lukasz Niemoczynski, Gregg Snedden
Summary: This study utilizes physics-based models and the bagged regression tree (BRT) machine learning algorithm to simulate wave dynamics at a living shoreline composed of constructed oyster reefs (CORs) in upper Delaware Bay. The results show that CORs effectively stabilize shorelines and reduce erosion. The BRT algorithm improves the model accuracy and identifies key processes responsible for simulation errors, providing important information for the assessment and adaptive management of CORs-based living shoreline restoration projects under climate change.
Letter
Gastroenterology & Hepatology
Stephan B. Dreyer, Rosie Upstill-Goddard, Assya Legrini, Andrew V. Biankin, Nigel B. Jamieson, David K. Chang, Sarah Allison, Andrew V. Biankin, Dario Beraldi, Euan Cameron, David K. Chang
Review
Microscopy
Georgina Fletcher, Kurt I. Anderson
Summary: Core Facilities and Technology Platforms play a crucial role in scientific research, but there is a lack of data to guide their development. In this survey, we examined 114 individuals working in 46 light microscopy facilities in the UK and explored issues related to career advancement, facility operations, and funding. The data revealed that these facilities serve as significant repositories of equipment and knowledge, adapting to meet local needs. Our report emphasizes the challenges faced by facility managers, institutions, and funders in evaluating facility performance and maximizing research funding returns.
JOURNAL OF MICROSCOPY
(2022)
Article
Oncology
John C. Dawson, Alison Munro, Kenneth Macleod, Morwenna Muir, Paul Timpson, Robert J. Williams, Margaret Frame, Valerie G. Brunton, Neil O. Carragher
Summary: Studied the mechanism of action of SRC inhibitor AZD0424 in cancer cells, confirming its ability to inhibit cell viability and reduce cell signaling pathway activation, especially with synergistic effects when used in combination with MEK inhibitors. Additionally, demonstrated that SRC inhibition is an effective way to block resistance to MEK inhibitors.
MOLECULAR ONCOLOGY
(2022)
Article
Multidisciplinary Sciences
Jaimy Jose, Monira Hoque, Johanna Engel, Syed S. Beevi, Mohamed Wahba, Mariya Ilieva Georgieva, Kendelle J. Murphy, William E. Hughes, Blake J. Cochran, Albert Lu, Francesc Tebar, Andrew J. Hoy, Paul Timpson, Kerry-Anne Rye, Carlos Enrich, Carles Rentero, Thomas Grewal
Summary: This study investigates the impact of low-density lipoproteins (LDL) on cell migration and invasion, as well as the intracellular transport pathways involving cholesterol. The results show that LDL stimulates cell migration and invasion, but blocking cholesterol export from late endosomes affects cell migration behavior. Additionally, AnxA6 is found to play a regulatory role in cell migration by promoting cholesterol delivery to focal adhesion structures.
SCIENTIFIC REPORTS
(2022)
Review
Biochemistry & Molecular Biology
Kendelle J. Murphy, Jessie Zhu, Michael Trpceski, Brooke A. Pereira, Paul Timpson, David Herrmann
Summary: The dense desmoplastic and fibrotic stroma in pancreatic ductal adenocarcinoma (PDAC) plays a role in disease progression and treatment response. Reciprocal interactions between the tumor and stroma through integrin-mediated signaling, particularly Focal Adhesion Kinase (FAK), are important in regulating stromal remodeling and tissue stiffness. Targeting the PDAC stroma, including transient stromal manipulation via short-term FAK inhibition, may enhance the vulnerability of PDAC cells to chemotherapy. This article highlights the significance of treatment timing and suggests further exploration of anti-fibrotic therapies for PDAC and other fibrotic diseases.
BIOCHEMICAL SOCIETY TRANSACTIONS
(2022)
Article
Multidisciplinary Sciences
Michael Papanicolaou, Amelia L. Parker, Michelle Yam, Elysse C. Filipe, Sunny Z. Wu, Jessica L. Chitty, Kaitlin Wyllie, Emmi Tran, Ellie Mok, Audrey Nadalini, Joanna N. Skhinas, Morghan C. Lucas, David Herrmann, Max Nobis, Brooke A. Pereira, Andrew M. K. Law, Lesley Castillo, Kendelle J. Murphy, Anaiis Zaratzian, Jordan F. Hastings, David R. Croucher, Elgene Lim, Brian G. Oliver, Fatima Valdes Mora, Benjamin L. Parker, David Gallego-Ortega, Alexander Swarbrick, Sandra O'Toole, Paul Timpson, Thomas R. Cox
Summary: The distribution and organization of matrix molecules, particularly collagen I, in the tumor stroma play an important role in breast cancer progression. Cancer-associated fibroblasts (CAFs) remodel the extracellular matrix (ECM) to promote or inhibit tumor growth. It has been found that CAF-secreted collagen XII can alter the organization of collagen I, creating a pro-invasive microenvironment that supports metastatic dissemination. Collagen XII may serve as an indicator of breast cancer patients at high risk of metastatic relapse.
NATURE COMMUNICATIONS
(2022)
Article
Oncology
Nicola S. Meagher, Kylie L. Gorringe, Matthew Wakefield, Adelyn Bolithon, Chi Nam Ignatius Pang, Derek S. Chiu, Michael S. Anglesio, Kylie-Ann Mallitt, Jennifer A. Doherty, Holly R. Harris, Joellen M. Schildkraut, Andrew Berchuck, Kara L. Cushing-Haugen, Ksenia Chezar, Angela Chou, Adeline Tan, Jennifer Alsop, Ellen Barlow, Matthias W. Beckmann, Jessica Boros, David D. L. Bowtell, Alison H. Brand, James D. Brenton, Ian Campbell, Dane Cheasley, Joshua Cohen, Cezary Cybulski, Esther Elishaev, Ramona Erber, Rhonda Farrell, Anna Fischer, Zhuxuan Fu, Blake Gilks, Anthony J. Gill, Charlie Gourley, Marcel Grube, Paul R. Harnett, Arndt Hartmann, Anusha Hettiaratchi, Claus K. Hogdall, Tomasz Huzarski, Anna Jakubowska, Mercedes Jimenez-Linan, Catherine J. Kennedy, Byoung-Gie Kim, Jae-Weon Kim, Jae-Hoon Kim, Kayla Klett, Jennifer M. Koziak, Tiffany Lai, Angela Laslavic, Jenny Lester, Yee Leung, Na Li, Winston Liauw, Belle W. X. Lim, Anna Linder, Jan Lubinski, Sakshi Mahale, Constantina Mateoiu, Simone McInerny, Janusz Menkiszak, Parham Minoo, Suzana Mittelstadt, David Morris, Sandra Orsulic, Sang-Yoon Park, Celeste Leigh Pearce, John Pearson, Malcolm C. Pike, Carmel M. Quinn, Ganendra Raj Mohan, Jianyu Rao, Marjorie J. Riggan, Matthias Ruebner, Stuart Salfinger, Clare L. Scott, Mitul Shah, Helen Steed, Colin J. R. Stewart, Deepak Subramanian, Soseul Sung, Katrina Tang, Paul Timpson, Robyn L. Ward, Rebekka Wiedenhoefer, Heather Thorne, Paul A. Cohen, Philip Crowe, Peter A. Fasching, Jacek Gronwald, Nicholas J. Hawkins, Estrid Hogdall, David G. Huntsman, Paul A. James, Beth Y. Karlan, Linda E. Kelemen, Stefan Kommoss, Gottfried E. Konecny, Francesmary Modugno, Sue K. Park, Annette Staebler, Karin Sundfeldt, Anna H. Wu, Aline Talhouk, Paul D. P. Pharoah, Lyndal Anderson, Anna DeFazio, Martin Kobel, Michael L. Friedlander, Susan J. Ramus
Summary: Clinical and gene expression data were analyzed to identify prognostic and diagnostic features of advanced-stage mucinous ovarian carcinoma (MOC) and differentiate it from gastrointestinal (GI) metastases. An infiltrative growth pattern was associated with poor prognosis, and high expression of THBS2 and TAGLN was linked to adverse prognosis in MOC. HER2 amplification or high mRNA expression was detected in some MOC cases, suggesting the potential of HER2-targeted therapy. MOC samples clustered with upper GI tumors, indicating similar biology and treatment strategies.
CLINICAL CANCER RESEARCH
(2022)
Article
Biochemical Research Methods
Jayne Culley, Peter W. Nagle, John C. Dawson, Neil O. Carragher
Summary: This article describes a method to recreate aspects of tumor biology using three-dimensional cell culture models that cannot be replicated by traditional two-dimensional techniques. The authors introduce a protocol for automated high throughput phenotypic profiling across panels of patient-derived glioma stem cell spheroid models. By using cell lines expressing the FUCCI cell cycle reporter, they demonstrate the monitoring of both live/dead cell endpoints and dynamic changes in the cell cycle. These assays provide additional insights into the mechanism of action of compound treatments, surpassing traditional cell viability assay endpoints.
Article
Multidisciplinary Sciences
Jordan F. Hastings, Sharissa L. Latham, Alvin Kamili, Madeleine S. Wheatley, Jeremy Z. R. Han, Marie Wong-Erasmus, Monica Phimmachanh, Max Nobis, Chiara Pantarelli, Antonia L. Cadell, Yolande E. I. O'Donnell, King Ho Leong, Sophie Lynn, Fan-Suo Geng, Lujing Cui, Sabrina Yan, Joanna Achinger-Kawecka, Clare Stirzaker, Murray D. Norris, Michelle Haber, Toby N. Trahair, Frank Speleman, Katleen De Preter, Mark J. Cowley, Ozren Bogdanovic, Paul Timpson, Thomas R. Cox, Walter Kolch, Jamie I. Fletcher, Dirk Fey, David R. Croucher
Summary: Gene expression noise promotes stochastic drug resistance in rare cancer cells. However, when integrated across multiple components of an apoptotic signaling network, the influence of noise leads to a higher frequency of chemoresistant neuroblastoma cells. These cells are characterized by JNK impairment and retain a memory of their resistant state even after chemotherapy treatment.
Article
Biochemical Research Methods
Gregory P. Way, Heba Sailem, Steven Shave, Richard Kasprowicz, Neil O. Carragher
Summary: The field of high content imaging has steadily evolved and expanded across many industry and academic research institutions since the early 1990s. High content imaging enables automated acquisition and analysis of microscopic images from various biological samples, allowing for large-scale screening and research on complex biological systems.
Article
Biochemical Research Methods
Steven Shave, John C. Dawson, Abdullah M. Athar, Cuong Q. Nguyen, Richard Kasprowicz, Neil O. Carragher
Summary: Phenonaut is a Python software package designed to address the data workflow needs of migration, control, integration, and auditability in the application of literature and proprietary techniques for data source and structure agnostic workflow creation.
Article
Biochemistry & Molecular Biology
Daniel J. Baillache, Teresa Valero, Alvaro Lorente-Macias, David Jonathan Bennett, Richard J. R. Elliott, Neil O. Carragher, Asier Unciti-Broceta
Summary: This study explored antiproliferative compounds for GBM treatment and established structure-antiproliferative activity relationships through the synthesis and screening of small compound libraries. Through a series of design, synthesis, and screening, potent CSF-1R inhibitors with significant antiproliferative activity were discovered.
RSC MEDICINAL CHEMISTRY
(2023)
Article
Biology
Kendelle J. Murphy, Daniel A. Reed, Cecilia R. Chambers, Jessie Zhu, Astrid Magenau, Brooke A. Pereira, Paul Timpson, David Herrmann
Summary: The extracellular matrix (ECM) plays a crucial role in regulating cellular function and tissue tension, with even subtle changes impacting disease development. Cell-derived matrix (CDM) models are valuable for studying cell-ECM interactions, offering insights into cancer progression.
Article
Multidisciplinary Sciences
Colinda L. G. J. Scheele, David Herrmann, Erika Yamashita, Cristina Lo Celso, Craig N. Jenne, Maja H. Oktay, David Entenberg, Peter Friedl, Roberto Weigert, Franck L. B. Meijboom, Masaru Ishii, Paul Timpson, Jacco van Rheenen
Summary: Studying the behavior and fate of individual cells in heterogeneous tissues is crucial for understanding disease development and treatment. Intravital microscopy techniques, such as multiphoton microscopy, provide a means to visualize the ultrastructure of live tissues and reveal insights into dynamic cell-cell interactions, as well as solutions for data reproducibility and handling large imaging datasets.
NATURE REVIEWS METHODS PRIMERS
(2022)