Article
Biochemistry & Molecular Biology
Grigor Varuzhanyan, Hsiuchen Chen, Rebecca Rojansky, Mark S. Ladinsky, J. Michael McCaffery, David C. Chan
Summary: The study revealed that Mff plays an essential role in regulating mitochondrial fragmentation and organization in mouse sperm development. Mutations in Mff resulted in mitochondrial abnormalities, affecting sperm morphology, motility, and fertility. Mitochondrial fission is crucial for maintaining mitochondrial organization during complex developmental processes.
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2021)
Article
Andrology
Opeyemi Olotu, Mark Dowling, David Homolka, Magdalena N. Wojtas, Panyi Tran, Tiina Lehtiniemi, Matteo Da Ros, Ramesh S. Pillai, Noora Kotaja
Summary: In this study, the function of the intermitochondrial cement (IMC) in piRNA biogenesis was clarified by identifying proteins that form complexes with PIWIL2/MILI. The two identified proteins, EXD1 and EXD2, were found to be involved in piRNA biogenesis and located in the IMC. Furthermore, the mutation of Exd2 exonuclease domain was shown to disrupt the regulation of specific pachytene piRNA clusters but not male fertility.
Article
Cell Biology
Elham Alzyoud, Viktor Vedelek, Zsuzsanna Rethi-Nagy, Zoltan Lipinszki, Rita Sinka
Summary: Microtubule nucleation in eukaryotes is primarily promoted by gamma-tubulin and the evolutionary conserved protein complex, gamma-Tubulin Ring Complex (gamma-TuRC). A tissue-specific gamma-TuRC has been identified in Drosophila, essential for male fertility. Testis-specific gene products, including t-gamma-TuRC, orchestrate the composition of gamma-TuRC during spermatogenesis and the formation of different post-meiotic microtubule organizing centers.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Multidisciplinary Sciences
Keisuke Shimada, Soojin Park, Haruhiko Miyata, Zhifeng Yu, Akane Morohoshi, Seiya Oura, Martin M. Matzuk, Masahito Ikawa
Summary: The study revealed the crucial role of ARMC12 in sperm mitochondrial sheath formation, with its absence leading to reduced sperm motility and male sterility. ARMC12, a mitochondrial peripheral membrane protein, functions by interacting with mitochondrial membrane proteins VDAC2 and VDAC3 to form the mitochondrial sheath.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Genetics & Heredity
Shihao Hong, Xiaozhong Shen, Jinmei Cheng, Hanyu Tang, Fei Sun
Summary: This study provides new insights into the molecular mechanisms of m6A modification in germ cell development and spermatogenesis by comprehensively analyzing MeRIP-seq and RNA-seq data. The findings suggest that m6A modification plays an important role in spermatogenesis and provide new information on the mechanism of m6A modification in gene expression regulation.
FRONTIERS IN GENETICS
(2022)
Article
Biochemistry & Molecular Biology
Wan-Sheng Liu, Chen Lu, Bhavesh V. Mistry
Summary: PRAME proteins, encoded by the PRAME gene family, are involved not only in transcriptional regulation in the nucleus but may also participate in nucleocytoplasmic transport and in the formation and function of germ cell-specific organelles during spermatogenesis.
CELL AND BIOSCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Tong He, Huanping Guo, Lin Xia, Xipeng Shen, Yun Huang, Xiao Wu, Xuelin Jiang, Yinying Xu, Yi Tan, Yunfang Zhang, Dongmei Tan
Summary: Hypoxia has been shown to potentially impair male fertility by causing spermatogenic dysfunction and decreased semen quality. This study investigated the molecular mechanisms via which RNA modifications influence spermatogenesis under hypoxic stress conditions. The results suggested that RNA modifications may be involved in cellular responses and provide a basis for understanding the molecular mechanisms underlying male infertility.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2022)
Article
Cell Biology
Rajakumar Anbazhagan, Raghuveer Kavarthapu, Ryan Dale, Kiersten Campbell, Fabio R. Faucz, Maria L. Dufau
Summary: GRTH/DDX25 is a testis-specific RNA helicase that plays a crucial role in spermatogenesis and male fertility. By analyzing mRNA-seq and miRNA-seq data, we identified several miRNAs, including miR146, miR122a, miR26a, miR27a, miR150, miR196a, and miR328, that are associated with spermatogenesis. These miRNAs target genes involved in various processes such as ubiquitination, chromatin remodeling, reversible protein phosphorylation, and acrosome stability. Our findings suggest that the post-transcriptional and translational regulation of germ-cell-specific mRNAs by miRNAs is important for spermatogenesis.
Article
Genetics & Heredity
Clara Gobe, Come Ialy-Radio, Remi Pierre, Julie Cocquet
Summary: In this study, a new spermatid-specific Cre transgenic mouse line was generated, which expresses the improved iCre recombinase under the control of the acrosomal vesicle protein 1 gene promoter. The expression of Cre protein was found to be restricted to the testis and detected only in round spermatids of stage V to VIII seminiferous tubules. The Acrv1-iCre line can efficiently conditionally knockout a gene during spermiogenesis with a >95% efficiency, providing a valuable tool to investigate gene function during the late stage of spermatogenesis.
Article
Biochemistry & Molecular Biology
Pui Yuen Chung, Keisuke Shoji, Natsuko Izumi, Yukihide Tomari
Summary: This study reveals that in silkworm cells, piRNA factors are actively compartmentalized into nuage and P-bodies, and this segregation requires the specific action of protein factors. Disruption of these actions leads to promiscuous overproduction of piRNAs deriving from non-transposable elements.
Article
Cell Biology
Sara Bilekova, Balma Garcia-Colomer, Alberto Cebrian-Serrano, Silvia Schirge, Karsten Krey, Michael Sterr, Thomas Kurth, Stefanie M. Hauck, Heiko Lickert
Summary: Spermatogenesis is a crucial biological process that produces functional sperm for successful reproduction. The acrosome, a lysosome-related organelle in the sperm head, is essential for egg-sperm recognition and fusion. The role of the insulin inhibitory receptor (inceptor) in acrosome formation was investigated, and its absence resulted in acrosomal malformation and male infertility.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Review
Cell Biology
Xiangchuan Wang, Boyi Hu, Zhongying Zhao, Yu Chung Tse
Summary: Researchers have uncovered the complex regulatory mechanisms of cytokinesis and cytoplasmic remodeling in the development of the Caenorhabditis elegans germline, highlighting the role of contractility proteins in faithful cell division and intercellular bridge stability.
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2022)
Article
Geriatrics & Gerontology
Heather E. Fice, Bernard Robaire
Summary: The effects of aging on the reproductive health of men and the consequences for their offspring are being recognized. Paternal age is correlated with genetic disorders in children. This study analyzed gene expression in round spermatids of young and aged rats and found increased expression of genes involved in sperm functions, as well as dysregulated genes related to germ cell epigenetic marks and communication. These findings contribute to understanding the genetic origin of fertility and epigenetic effects with advanced paternal aging.
EXPERIMENTAL GERONTOLOGY
(2023)
Review
Endocrinology & Metabolism
Mengchao He, Kun Liu, Jia Cao, Qing Chen
Summary: This review summarizes the current knowledge about the role and potential mechanisms of clock genes in regulating spermatogenesis. Rhythmic oscillation in semen parameters and physiological events of spermatogenesis have been observed. Disturbances in clock gene expression due to genetic mutations or environmental changes can significantly impair spermatogenesis, although the mechanisms of spermatogenesis regulation by clock genes remain unclear.
REVIEWS IN ENDOCRINE & METABOLIC DISORDERS
(2023)
Article
Multidisciplinary Sciences
Odei Barrenada, Eduardo Larriba, Daniel Fernandez-Perez, Miguel angel Brieno-Enriquez, Jesus del Mazo Martinez
Summary: Recent studies have shown that mitochondrial small noncoding RNAs (sncRNAs) play important roles in germ cell development and differentiation. By analyzing sequencing data, we identified the origins, expression levels, and potential functions of piRNAs, as well as their association with other sncRNAs and the mitochondrial regulatory region. Our findings suggest that mitochondria-associated piRNAs may mediate nucleo-mitochondrial communication.
SCIENTIFIC REPORTS
(2022)
Article
Hematology
Yu-Tzu Tai, Chirag Acharya, Gang An, Michele Moschetta, Mike Y. Zhong, Xiaoyan Feng, Michele Cea, Antonia Cagnetta, Kenneth Wen, Hans van Eenennaam, Andrea van Elsas, Lugui Qiu, Paul Richardson, Nikhil Munshi, Kenneth C. Anderson
Article
Biotechnology & Applied Microbiology
Chen Chen, Iris Pla-Palacin, Pedro M. Baptista, Peng Shang, Loes A. Oosterhoff, Monique E. van Wolferen, Louis C. Penning, Niels Geijsen, Bart Spee
BIOTECHNOLOGY AND BIOENGINEERING
(2018)
Article
Oncology
Deepak Mittal, Dipti Vijayan, Joost Neijssen, Joost Kreijtz, Maurice M. J. M. Habraken, Hans Van Eenennaam, Andrea Van Elsas, Mark J. Smyth
Article
Multidisciplinary Sciences
Bas Castelijns, Mirna L. Baak, Ilia S. Timpanaro, Caroline R. M. Wiggers, Marit W. Vermunt, Peng Shang, Ivanela Kondova, Geert Geeven, Valerio Bianchi, Wouter de Laat, Niels Geijsen, Menno P. Creyghton
NATURE COMMUNICATIONS
(2020)
Article
Oncology
Erik Voets, Marc Parade, David Lutje Hulsik, Sanne Spijkers, Wout Janssen, Joost Rens, Inge Reinieren-Beeren, Gilbert van den Tillaart, Sander van Duijnhoven, Lilian Driessen, Maurice Habraken, Peter van Zandvoort, Joost Kreijtz, Paul Vink, Andrea van Elsas, Hans van Eenennaam
JOURNAL FOR IMMUNOTHERAPY OF CANCER
(2019)
Article
Oncology
Beth Hutchins, Gary C. Starling, Mark A. McCoy, Danuta Herzyk, Frederique M. Poulet, John Dulos, Liming Liu, Soonmo Peter Kang, Laurence Fayadat-Dilman, Mark Hsieh, Christine L. Andrews, Gulesi Ayanoglu, Constance Cullen, Rene de Waal Malefyt, Robert A. Kastelein, Sabine Le Saux, Julie Lee, Sophie Li, Dan Malashock, Svetlana Sadekova, George Soder, Hans van Eenennaam, Aarron Willingham, Ying Yu, Michel Streuli, Gregory J. Carven, Andrea van Elsas
MOLECULAR CANCER THERAPEUTICS
(2020)
Article
Immunology
Julia Busselaar, Sun Tian, Hans van Eenennaam, Jannie Borst
FRONTIERS IN IMMUNOLOGY
(2020)
Article
Chemistry, Multidisciplinary
Glenn A. O. Cremers, Bas J. H. M. Rosier, Ab Meijs, Nicholas B. Tito, Sander M. J. van Duijnhoven, Hans van Eenennaam, Lorenzo Albertazzi, Tom F. A. de Greef
Summary: The study investigated the tunable design parameters of antibody-functionalized DNA nanostructures binding to therapeutically relevant receptors, revealing key insights into receptor binding efficiency governed mainly by nanostructure size and DNA handle location.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2021)
Article
Biochemistry & Molecular Biology
Zhihan Zhao, Peng Shang, Fanny Sage, Niels Geijsen
Summary: CRISPR/Cas12a is a nuclease that generates sticky-ended DNA double strand breaks, which can be utilized for precise genome editing through a strategy called 'Ligation-Assisted Homologous Recombination' (LAHR). LAHR achieves high editing efficiency by using a short double-stranded DNA repair template with sticky ends and homologous arms. This study expands the repertoire of genome editing technologies and provides insights into DNA repair mechanisms involved in genome editing.
NUCLEIC ACIDS RESEARCH
(2022)
Article
Genetics & Heredity
Thomas Swartjes, Peng Shang, Dennis T. M. van den Berg, Tim Kunne, Niels Geijsen, Stan J. J. Brouns, John van der Oost, Raymond H. J. Staals, Richard A. Notebaart
Summary: This study demonstrates that DNA oligonucleotides can effectively inhibit the activity of Cas9, reducing both on- and off-target cleavage in genome editing applications.
Article
Oncology
Lars Guelen, Thierry O. Fischmann, Jerelyn Wong, Smita Mauze, Marco Guadagnoli, Nikolina Babala, Jozef Wagenaars, Veronica Juan, David Rosen, Winnie Prosise, Maurice Habraken, Imke Lodewijks, Danling Gu, Judith Stammen-Vogelzangs, Ying Yu, Jeanne Baker, David Lutje Hulsik, Lilian Driessen-Engels, Dan Malashock, Joost Kreijtz, Astrid Bertens, Evert de Vries, Astrid Bovens, Arne Bramer, Yiwei Zhang, Richard Wnek, Sean Troth, Elliot Chartash, Konstantin Dobrenkov, Svetlana Sadekova, Andrea van Elsas, Jason K. Cheung, Laurence Fayadat-Dilman, Jannie Borst, Amy M. Beebe, Hans Van Eenennaam
Summary: MK-5890, a novel CD27 agonistic antibody, has the potential to complement PD-1 checkpoint inhibition in cancer immunotherapy and is currently undergoing clinical evaluation.
JOURNAL FOR IMMUNOTHERAPY OF CANCER
(2022)
Article
Biochemical Research Methods
Erik van Buijtenen, Wout Janssen, Paul Vink, Maurice J. M. Habraken, Laura J. A. Wingens, Andrea van Elsas, Wilhelm T. S. Huck, Jessie A. G. L. van Buggenum, Hans van Eenennaam
Summary: Researchers integrated multi-omics single-cell sequencing technologies with an in vitro differentiation method to analyze the phenotypic characteristics of individual cells, revealing differences in immunoglobulin subclass-specific surface markers, transcriptional profiles, and signaling transduction pathway components. They also identified differential expression and sensitivity to cytokine stimulation in different ASCs, highlighting the importance of SYK, NF-kappa B, and mTOR activity in plasma cell differentiation and IgG secretion. This multi-omics approach provides valuable insights into human ASCs in healthy and diseased samples, and offers a useful tool for identifying novel biomarkers and potential drug targets.
MOLECULAR & CELLULAR PROTEOMICS
(2023)
Article
Multidisciplinary Sciences
Xin Lei, Indu Khatri, Tom de Wit, Iris de Rink, Marja Nieuwland, Ron Kerkhoven, Hans van Eenennaam, Chong Sun, Abhishek D. Garg, Jannie Borst, Yanling Xiao
Summary: Despite their low abundance, cDC1s play a crucial role in anti-cancer immunity and are positively associated with patient survival. This study demonstrates that contact with activated CD4(+) T-cells enables cDC1s to induce a CTL response. The transcriptomic signature of helped cDC1s correlates with tumor infiltration by CTLs, Thelper(h)-1 cells, and overall survival.
NATURE COMMUNICATIONS
(2023)
Article
Oncology
Arjan Kol, Xiaoyu Fan, Marta. A. A. Wazynska, Sander M. J. van Duijnhoven, Danique Giesen, Annechien Plat, Hans Van Eenennaam, Philip. H. H. Elsinga, Hans. W. W. Nijman, Marco de Bruyn
Summary: This study focuses on generating and radiolabeling monoclonal antibodies targeting human CD103 for non-invasive immune PET imaging, demonstrating their potential as biomarkers for effective anticancer immune responses. The results show high specificity and sensitivity of the CD103 immuno-PET tracers in visualizing T cell infiltration, indicating their suitability for future applications in cancer immunotherapy assessment.
JOURNAL FOR IMMUNOTHERAPY OF CANCER
(2022)
Article
Biochemical Research Methods
Francesca Rivello, Erik van Buijtenen, Kinga Matula, Jessie A. G. L. van Buggenum, Paul Vink, Hans van Eenennaam, Klaas W. Mulder, Wilhelm T. S. Huck
Summary: This study introduces a new single-cell multi-omics method, QuRIE-seq, for quantifying RNA and intracellular and extracellular proteins, revealing changes in signaling networks and gene expression profiles at different time scales, and the impact of an inhibitory drug on signaling pathways and gene expression profiles.
CELL REPORTS METHODS
(2021)