Article
Neurosciences
Che-Wei Chang, Yu-Tien Hsiao, Meyer B. Jackson
Summary: This study demonstrates that Synaptophysin (Syp) and synaptogyrin (Syg) play a regulatory role in dense-core vesicle function at various stages, affecting fusion initiation, mode selection between full fusion and kiss-and-run, and influencing the dynamics of both initial and late-stage fusion pores. The transmembrane domain (TMD) of Syp influences small initial fusion pores, while the C-terminal domain influences large late-stage fusion pores, possibly through an interaction with dynamin.
JOURNAL OF NEUROSCIENCE
(2021)
Article
Biology
Manindra Bera, Sathish Ramakrishnan, Jeff Coleman, Shyam S. Krishnakumar, James E. Rothman
Summary: In neurotransmitter release, Complexin regulates rapid vesicle fusion by clamping the SNARE complex, with a fusion delay likely induced by Synaptotagmin-1. The accessory-central domains of Complexin are crucial for its inhibitory function, with specific interactions with SNAREpins enhancing its effectiveness. The C-terminal domain promotes clamping by interacting with membranes to locally elevate Complexin concentration.
Article
Multidisciplinary Sciences
Pengcheng Zhang, David Rumschitzki, Robert H. Edwards
Summary: This study investigates the release of secretory vesicles in mouse adrenal chromaffin cells and discovers diverse modes of release. Through comprehensive analysis, it is revealed that the variations in release cannot be solely explained by stochasticity, but involve distinct mechanisms. Furthermore, the loss of calcium sensor synaptotagmin 7 increases the proportion of slow events without altering the intrinsic properties of either class, suggesting the potential for independent regulation.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Biochemistry & Molecular Biology
Emily M. Grasso, Mayu S. Terakawa, Alex L. Lai, Ying Xue Xie, Trudy F. Ramlall, Jack H. Freed, David Eliezer
Summary: Complexins are important regulators of SNARE-mediated exocytosis of synaptic vesicles. The structural and functional characterization of mCpx1 C-terminal domain (CTD) reveals differences in inhibitory function compared to the worm counterpart wCpx1 CTD. The mCpx1 CTD shows structural divergences in regions critical for inhibiting spontaneous fusion of synaptic vesicles, suggesting a potential basis for evolutionary divergences in complexin function.
JOURNAL OF MOLECULAR BIOLOGY
(2023)
Article
Cell Biology
Guoyi Yang, Liuju Li, Yanmei Liu, Kuo Liang, Lisi Wei, Liangyi Chen
Summary: Impaired insulin release in type 2 diabetes is closely related to chronically elevated glucose concentrations. This study found that high glucose delays fusion events, reduces full fusion, and decreases the size of fusion pores, all contributing to compromised insulin secretion.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Neurosciences
Hui-Ju Yang, Pin-Chun Chen, Chien-Ting Huang, Tzu-Lin Cheng, Sheng-Ping Hsu, Chien-Yu Chen, Juu-Chin Lu, Chih-Tien Wang
Summary: The study demonstrated that Syn Ia regulates DCV exocytosis through phosphorylation, with a decrease in secretion rate observed when phosphodeficient Syn Ia is overexpressed. Additionally, Syn Ia may modulate the fusion pore dynamics of DCVs via specific phosphorylation sites, potentially interacting with proteins involved in fusion pore regulation. Immunoprecipitation and proximity ligation assays confirmed the phosphorylation-dependent interaction of Syn Ia with proteins like Syp on DCVs, highlighting a phosphorylation-mediated regulatory mechanism.
JOURNAL OF NEUROSCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Josh Pierson, Yeon-Kyun Shin
Summary: This study investigated the influence of complexin-1 (cpx1) on the expansion of SNARE-dependent fusion pores in neurotransmitter release. The results showed that cpx1 significantly increased the probability of large fusion pore formation and promoted faster release of a polymer probe. This demonstrated a reciprocal relationship between event duration and the size of the fusion pore.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2021)
Article
Plant Sciences
Martiniano Maria Ricardi, Niklas Wallmeroth, Cecilia Cermesoni, Dietmar Gerald Mehlhorn, Sandra Richter, Lei Zhang, Josephine Mittendorf, Ingeborg Godehardt, Kenneth Wayne Berendzen, Edda von Roepenack-Lahaye, York-Dieter Stierhof, Volker Lipka, Gerd Juergens, Christopher Grefen
Summary: The final step in secretion is membrane fusion facilitated by SNARE proteins that reside in opposite membranes. The formation of a trans-SNARE complex between one R and three Q coiled-coiled SNARE domains drives the final approach of the membranes providing the mechanical energy for fusion. Biological control of this mechanism is exerted by additional domains within some SNAREs.
Article
Cell Biology
Micaela Vanina Buzzatto, Maria Victoria Berberian, Ary Lautaro Di Bartolo, Diego Masone, Claudia Nora Tomes
Summary: The sperm acrosome releases its contents through fusion pores between the acrosomal and plasma membranes, and the dilation of these pores leads to the release of membranes and granule contents. α-Synuclein, a cytosolic protein, is found in the acrosomal domain of human sperm and is required for acrosomal release. Stabilization of fusion pores is responsible for the secretion blockage.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Multidisciplinary Sciences
Rohith K. Nellikka, Bhavya R. Bhaskar, Kinjal Sanghrajka, Swapnali S. Patil, Debasis Das
Summary: Alpha-synuclein plays a crucial role in the pathogenesis of Parkinson's disease by directly regulating individual exocytotic release events, controlling vesicular secretion. It forms an inhibitory complex to decrease the open probability of fusion pores, influenced by various factors. This study reveals the key role of alpha-synuclein in modulating pore properties and highlights its regulatory action in membrane fusion.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Biology
Sebastien Houy, Joana S. Martins, Noa Lipstein, Jakob Balslev Sorensen
Summary: This study investigated the relationship between phorbolesters and Munc13-1 and ubMunc13-2, and found that phorbolesters only stimulated secretion when ubMunc13-2 was dominant. The results showed that DAG/phorbolesters, ubMunc13-2, and Syt7 are important factors for stimulating dense-core vesicle priming.
Article
Neurosciences
Joshua Brady, Alexander Vasin, Maria Bykhovskaia
Summary: The study developed and validated an atomic model of the Cpx-mediated clamped state of the SNARE complex, using a combination of electrophysiology, molecular modeling, and site-directed mutagenesis in Drosophila. The research revealed that the clamped state of the SNARE complex has two or three unraveled helical turns of synaptobrevin (Syb), and that Cpx's clamping function is predominantly maintained by its accessory helix (AH). Molecular modeling suggested that the Cpx AH interacts with the unraveled C terminus of Syb and the SV lipid bilayer.
Review
Biology
Matjaz Stenovec
Summary: Ketamine exerts rapid and potent antidepressant effects by elevating intracellular cAMP concentration in astrocytes, affecting astrocyte calcium signaling, and hindering vesicle fusion pore configuration. It also impacts astrocyte control of extracellular K+ and redistributes cholesterol in astrocyte membranes, potentially modulating processes contributing to its antidepressant action.
Article
Computer Science, Artificial Intelligence
Szymon Szczesny, Pawel Pietrzak
Summary: This study introduces a system for monitoring vesicle fusions during the exocytosis process, aiming to detect precancerous conditions in cells. Utilizing multiple small perceptron neural networks in parallel on the Jetson TX2 platform, it can process approximately 140,000 sensors in real time with 99% accuracy.
NEURAL COMPUTING & APPLICATIONS
(2022)
Article
Chemistry, Multidisciplinary
Xiulan He, Andrew G. Ewing
Summary: This study examined the temporal and concentration effects of chemical stimulation on exocytotic molecular plasticity. The results showed that catecholamine release and release dynamics depended on the concentration of the stimulant solution. Increased release was observed at 30 and 50 mM stimulation, while decreased release was observed at 100 mM stimulation. These findings suggest a possible link between stimulation intensity and synaptic (or adrenal) plasticity.
Article
Cell Biology
Hsin-Fang Chang, Stefanie Mannebach, Andreas Beck, Keerthana Ravichandran, Elmar Krause, Katja Frohnweiler, Claudia Fecher-Trost, Claudia Schirra, Varsha Pattu, Veit Flockerzi, Jens Rettig
JOURNAL OF CELL BIOLOGY
(2018)
Article
Neurosciences
Ali H. Shaib, Angelina Staudt, Ali Harb, Margarete Klose, Ahmed Shaaban, Claudia Schirra, Ralf Mohrmann, Jens Rettig, Ute Becherer
FRONTIERS IN CELLULAR NEUROSCIENCE
(2018)
Article
Neurosciences
Olga Ratai, Claudia Schirra, Elvin Rajabov, Irene Brunk, Gudrun Ahnert-Hilger, Praneeth Chitirala, Ute Becherer, David R. Stevens, Jens Rettig
JOURNAL OF NEUROSCIENCE
(2019)
Article
Cell Biology
Nicole Scholz, Nadine Ehmann, Divya Sachidanandan, Cordelia Imig, Benjamin H. Cooper, Olaf Jahn, Kerstin Reim, Nils Brose, Jutta Meyer, Marius Lamberty, Steffen Altrichter, Anne Bormann, Stefan Hallermann, Martin Pauli, Manfred Heckmann, Christian Stigloher, Tobias Langenhan, Robert J. Kittel
JOURNAL OF CELL BIOLOGY
(2019)
Article
Cell Biology
Francisco Jose Lopez-Murcia, Kerstin Reim, Olaf Jahn, Holger Taschenberger, Nils Brose
Article
Immunology
Praneeth Chitirala, Keerthana Ravichandran, Donatella Galgano, Marwa Sleiman, Elmar Krause, Yenan T. Bryceson, Jens Rettig
FRONTIERS IN IMMUNOLOGY
(2019)
Article
Multidisciplinary Sciences
Hao Jiang, Thomas J. Esparza, Terrance T. Kummer, Haining Zhong, Jens Rettig, David L. Brody
SCIENTIFIC REPORTS
(2020)
Article
Biochemistry & Molecular Biology
Michael Estl, Pascal Blatt, Xuemei Li, Ute Becherer, Hsin-Fang Chang, Jens Rettig, Varsha Pattu
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Article
Immunology
Praneeth Chitirala, Keerthana Ravichandran, Claudia Schirra, Hsin-Fang Chang, Elmar Krause, Uli Kazmaier, Marcel A. Lauterbach, Jens Rettig
JOURNAL OF IMMUNOLOGY
(2020)
Article
Immunology
Marwa Sleiman, David R. Stevens, Praneeth Chitirala, Jens Rettig
FRONTIERS IN IMMUNOLOGY
(2020)
Editorial Material
Cell Biology
Jens Rettig, Cosima T. Baldari
IMMUNOLOGY AND CELL BIOLOGY
(2020)
Article
Biochemistry & Molecular Biology
Hsin-Fang Chang, Marie-Louise Wirkner, Elmar Krause, Jens Rettig
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Article
Biology
Praneeth Chitirala, Hsin-Fang Chang, Paloma Martzloff, Christiane Harenberg, Keerthana Ravichandran, Midhat H. Abdulreda, Per-Olof Berggren, Elmar Krause, Claudia Schirra, Trese Leinders-Zufall, Fritz Benseler, Nils Brose, Jens Rettig
Article
Immunology
Donatella Galgano, Tayebeh Soheili, Matthias Voss, Lamberto Torralba-Raga, Bianca Tesi, Frank Cichocki, Isabelle Andre, Jens Rettig, Marina Cavazzana, Yenan Bryceson
FRONTIERS IN IMMUNOLOGY
(2020)
Article
Biochemistry & Molecular Biology
Uwe Thorsten Lux, Johanna Ehrenberg, Anneka Joachimsthaler, Jenny Atorf, Bianca Pircher, Kerstin Reim, Jan Kremers, Andreas Giessl, Johann Helmut Brandstaetter
Summary: The study found that Cplx 2, not Cplx 1, is the main isoform expressed in mouse retinas, and knockout of Cplx 1 altered retinal function. These results provide an important basis for future research on the function of Cplxs 1 and 2 in processing visual signals in the mammalian retina.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)