Article
Multidisciplinary Sciences
Stephanie Lebreton, Simona Paladino, Dandan Liu, Maria Nitti, Julia von Blume, Paolo Pinton, Chiara Zurzolo
Summary: The levels of calcium within cisternae directly affect GPI-AP homocluster formation in the Golgi, with TGN calcium/manganese pump SPCA1 and calcium-binding protein Cab45 being essential for this process. Down regulation of SPCA1 or Cab45 leads to impaired GPI-AP oligomerization in the Golgi and missorting to the basolateral surface in polarized epithelial cells.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Endocrinology & Metabolism
Petrina Koumarianou, Celia Fernandez-Mendez, Danae Fajardo-Delgado, Lidia Mirella Mielu, Pilar Santisteban, Antonio De la Vieja
Summary: This study reveals the involvement of the clathrin-coated machinery in basolateral trafficking of NIS (sodium/iodide symporter), providing further understanding of NIS subcellular sorting mechanisms. These findings will help uncover new targets in thyroid cancer treatment.
Article
Neurosciences
Lars Binkle, Marcel Klein, Uwe Borgmeyer, Dietmar Kuhl, Guido Hermey
Summary: SorLA is an important receptor involved in neuronal endosomal sorting, with the ability to interact with various ligands, including those related to Alzheimer's disease. It plays a crucial role in neuronal endosomal sorting mechanisms and may function as a multifunctional receptor with intracellular sorting capacities.
Article
Biology
Ritvija Agrawal, John P. Gillies, Juliana L. Zang, Jingjing Zhang, Sharon R. Garrott, Hiroki Shibuya, Jayakrishnan Nandakumar, Morgan E. DeSantis
Summary: This study identifies KASH5 as the first transmembrane dynein activating adaptor and provides molecular insights into how it activates dynein during meiosis.
Review
Biochemistry & Molecular Biology
Ivana Strazic Geljic, Paola Kucan Brlic, Lucija Musak, Dubravka Karner, Andreja Ambriovic-Ristov, Stipan Jonjic, Peter Schu, Tihana Lenac Rovis
Summary: Viruses manipulate cellular protein trafficking pathways, particularly adaptor protein complexes (AP), to facilitate viral replication and evade host immune defenses. The interactions between viruses and AP complexes are found across major viral families, with well-documented cases in human immunodeficiency virus and human herpesviruses. High priority disease-causing agents like Ebola, Nipah, and SARS-CoV-2 underscore the importance of further understanding virus-AP interplay for designing novel antiviral therapies.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Yinsuo Zhao, Eli Song, Wenjuan Wang, Chung-Han Hsieh, Xinnan Wang, Wei Feng, Xiangming Wang, Kang Shen
Summary: The trafficking of mitochondria into dendrites and axons is essential in the physiology and pathophysiology of neurons. Metaxin-1 and metaxin-2 play a crucial role in mitochondrial transport, forming complexes with Miro and motor proteins to facilitate movement along microtubules. These proteins have evolutionarily conserved functions and are required for proper mitochondrial trafficking in both C. elegans and human neurons.
NATURE COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
David Akopian, Colleen A. McGourty, Michael Rape
Summary: Cuilin-RING E3 ligases (CRLs) are essential ubiquitylation enzymes and the formation of CRL complexes is a tightly regulated process, with co-adaptors playing an important role in CRL assembly.
Review
Cell Biology
Mabruka Alfaidi, Matthew L. Scott, Anthony Wayne Orr
Summary: The Nck family of adaptor proteins, Nck1 and Nck2, play crucial roles in linking phosphotyrosine signaling to changes in cytoskeletal dynamics and gene expression, affecting cellular phenotype. While they have redundant roles in vascular development and angiogenesis, recent studies suggest that Nck1 and Nck2 may differentially regulate cell phenotype in the adult vasculature. Domain-specific interactions likely mediate these isoform-selective effects, and could potentially be targeted for therapeutic interventions.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biochemical Research Methods
Wentao Gao, Dali Xu, Hongfei Li, Junping Du, Guohua Wang, Dan Li
Summary: Adaptor proteins play an important role in signal transduction pathways and are closely related to diseases. Previous studies used PSSM and neural network methods to identify adaptor proteins, but ordinary neural networks cannot handle the contextual information in PSSM profiles well. This study proposes an efficient method combining 1-D CNN and biLSTM, which shows better performance in identifying adaptor proteins.
Review
Cell Biology
Masatoshi Takeichi
Summary: Animal tissues are comprised of diverse cells that need to be sorted into different tissue compartments for organogenesis. This sorting process is achieved by cells themselves, as they can self-organize into tissue-like structures in vitro. Cell sorting is regulated by biochemical mechanisms, such as adhesive proteins that connect cells based on their specific binding properties, and physical forces, like cell-cortical tension, that control cell cohesion and assembly patterns. This article focuses on the role of the 'cadherin' family of adhesion molecules in cell sorting, either independently or in cooperation with other factors. It also discusses novel ideas beyond traditional models of cell sorting.
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2023)
Review
Chemistry, Multidisciplinary
Junlie Yao, Aoran Zhang, Yue Qiu, Zihou Li, Xiaoxia Wu, Zhouhua Li, Aiguo Wu, Fang Yang
Summary: This review focuses on the recent representative modalities of emerging Zn-involved oncology nanomedicine, mainly emphasizing the rational design, biological effect and biosafety, and therapeutic strategies. The underlying critical obstacles and future perspectives of Zn-involved oncology nanomedicines, primarily focusing on the chances and challenges of clinical translation, are also discussed.
Article
Biochemistry & Molecular Biology
Halesha D. Basavarajappa, Jose M. Irimia, Brandon M. Bauer, Patrick T. Fueger
Summary: Avoiding the loss of functional beta cell mass is crucial for diabetes prevention or treatment. This study investigated the molecular mechanisms linking diabetogenic stimuli to beta cell death and identified the dynamic interaction between Mig6 and NumbL. Under glucolipotoxic conditions, siRNA-mediated suppression of NumbL expression prevented apoptosis by blocking NF-KB signaling activation. The interactions among Mig6, NumbL, and TRAF6 activated pro-apoptotic NF-KB signaling and inhibited pro-survival EGF signaling, leading to beta cell apoptosis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Multidisciplinary Sciences
Carley Snoznik, Valentina Medvedeva, Jelena Mojsilovic-Petrovic, Paige Rudich, James Oosten, Robert G. Kalb, Todd Lamitina
Summary: The SPOP protein plays a crucial role in regulating diseases associated with the C9orf72 gene. Inhibiting SPOP can alleviate the toxicity of DPR proteins, potentially offering a new therapeutic approach for treating ALS and FTD.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Plant Sciences
Woo-Jong Hong, Eui-Jung Kim, Jinmi Yoon, Jeniffer Silva, Sunok Moon, Cheol Woo Min, Lae-Hyeon Cho, Sun Tae Kim, Soon Ki Park, Yu-Jin Kim, Ki-Hong Jung
Summary: TAPE is an essential myosin XI adaptor in rice pollen tube elongation, and its loss can affect the development of pollen tubes and reproductive ability of plants.
Article
Cell Biology
Shimin Wang, Longfeng Yao, Wenjuan Zhang, Zihang Cheng, Can Hu, Hang Liu, Yanling Yan, Anbing Shi
Summary: The deficiency of SMAP-1 leads to mislocalization of apical and basolateral proteins on the trans-Golgi network (TGN), with SMAP-1 colocalizing with AP-1. The AP-1 complex regulates the TGN localization of SMAP-1, promoting the assembly of clathrin to ensure polarized conventional secretion in C. elegans intestinal epithelia.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Carlos M. Guardia, Raffaella De Pace, Aritra Sen, Amra Saric, Michal Jarnik, David A. Kolin, Ambarish Kunwar, Juan S. Bonifacino
Article
Virology
Elodie Mailler, Abdul A. Waheed, Sang-Yoon Park, David C. Gershlick, Eric O. Freed, Juan S. Bonifacino
Article
Cell Biology
Morie Ishida, Juan S. Bonifacino
JOURNAL OF CELL BIOLOGY
(2019)
Article
Multidisciplinary Sciences
Qi Zhang, Yue Xu, Juhyung Lee, Michal Jarnik, Xufeng Wu, Juan S. Bonifacino, Jingshi Shen, Yihong Ye
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2020)
Review
Biochemistry & Molecular Biology
Rafael Mattera, Raffaella De Pace, Juan S. Bonifacino
BIOCHEMICAL SOCIETY TRANSACTIONS
(2020)
Article
Multidisciplinary Sciences
Amra Saric, Spencer A. Freeman, Chad D. Williamson, Michal Jarnik, Carlos M. Guardia, Michael S. Fernandopulle, David C. Gershlick, Juan S. Bonifacino
Summary: Endolysosomes are tethered to the endoplasmic reticulum (ER) by the sorting nexin SNX19, reducing their motility and concentrating them in the perinuclear area. This mechanism involves specific protein domains and contributes to the regulation of organelle dynamics.
NATURE COMMUNICATIONS
(2021)
Article
Multidisciplinary Sciences
Elodie Mailler, Carlos M. Guardia, Xiaofei Bai, Michal Jarnik, Chad D. Williamson, Yan Li, Nunziata Maio, Andy Golden, Juan S. Bonifacino
Summary: ATG9A is a transmembrane protein involved in lipid mobilization, essential for autophagosome expansion, mitochondrial fatty acid import, and beta-oxidation. Depletion of ATG9A inhibits autophagy, increases lipid droplet size/number, and blocks transfer of fatty acids to mitochondria. ATG9A localizes to vesicular-tubular clusters associated with TMEM41B and plays a critical role in lipid mobilization from lipid droplets to autophagosomes and mitochondria.
NATURE COMMUNICATIONS
(2021)
Article
Cell Biology
Tanveer Ahmad, Detlef Vullhorst, Rituparna Chaudhuri, Carlos M. Guardia, Nisha Chaudhary, Irina Karavanova, Juan S. Bonifacino, Andres Buonanno
Summary: This study investigates the processing and sorting of NRG3 in neurons. It finds that NRG3 is cleaved in the trans-Golgi network and released, and then re-endocytosed and transported into axons. NRG3 accumulates at presynaptic terminals through stable interaction with its receptor. This trans-synaptic retention mechanism may explain the polarized expression of other neuronal transmembrane ligands and receptors.
JOURNAL OF CELL BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Tal Keren-Kaplan, Amra Saric, Saikat Ghosh, Chad D. Williamson, Rui Jia, Yan Li, Juan S. Bonifacino
Summary: This study identifies RUFY3 and RUFY4 as ARL8 effectors that promote retrograde transport of endolysosomes along microtubules by interacting with dynein-dynactin. RUFY3 and RUFY4 also play a role in concentration and redistribution of endolysosomes in different cellular contexts. These findings highlight the importance of ARL8 in regulating both anterograde and retrograde transport of endolysosomes.
NATURE COMMUNICATIONS
(2022)
Article
Cell Biology
Rafael Mattera, Raffaella De Pace, Juan S. Bonifacino
Summary: Adaptor protein 4 (AP-4) is a complex that binds with AAGAB to promote its assembly and plays a role in the development of the central nervous system.
MOLECULAR BIOLOGY OF THE CELL
(2022)
Article
Cell Biology
Juan S. Bonifacino
Summary: This passage describes the application of electron microscopy in studying eukaryotic cells and the importance of this development in understanding the internal organization and function of these cells. The author's long-term research focuses on protein sorting and organelle transport in the cytoplasm, and has revealed various mechanisms and defects leading to human diseases.
MOLECULAR BIOLOGY OF THE CELL
(2022)
Article
Cell Biology
Ariane Schleinitz, Lara-Alina Poettgen, Tal Keren-Kaplan, Jing Pu, Paul Saftig, Juan S. Bonifacino, Albert Haas, Andreas Jeschke
Summary: This study biochemically characterized endosomes purified from untreated or experimentally manipulated cells to establish the functional interdependence of HOPS and interacting proteins. The results showed that Rab2a and Rab7 are present in late endosomes, while Arl8 and BORC are present in lysosomes, and HOPS drives LE-LYS fusion by bridging late endosomal Rab2a with lysosomal BORC-anchored Arl8. Additionally, Rab7 promotes fusion by moving LEs towards LYSs via dynein.
Article
Cell Biology
Ganesh Vilas Shelke, Chad D. Williamson, Michal Jarnik, Juan S. Bonifacino
Summary: This study shows that disruption of the BORC-ARL8-HOPS pathway increases exosome secretion by impairing fusion of multivesicular endosomes with lysosomes. This leads to the release of intraluminal vesicles into the extracellular space. Therefore, suppressing the BORC-ARL8-HOPS pathway could be used to enhance exosome yields.
JOURNAL OF CELL BIOLOGY
(2023)
Article
Medicine, Research & Experimental
Xin Chen, Thomas Dong, Yuhui Hu, Raffaella De Pace, Rafael Mattera, Kathrin Eberhardt, Marvin Ziegler, Terry Pirovolakis, Mustafa Sahin, Juan S. Bonifacino, Darius Ebrahimi-Fakhari, Steven J. Gray
Summary: Spastic paraplegia 50 (SPG50) is an ultrarare childhood-onset neurological disorder caused by biallelic loss-of-function variants in the AP4M1 gene. In preclinical studies, adeno-associated virus (AAV)/AP4M1 gene therapy showed promising results in rescuing the symptoms of SPG50. The therapy was effective in transducing patient-derived fibroblasts and demonstrated dose-dependent effects in Ap4m1-KO mice, supporting the potential for a clinical trial in treating SPG50 through intrathecal administration of AAV9/AP4M1.
JOURNAL OF CLINICAL INVESTIGATION
(2023)
Article
Biochemistry & Molecular Biology
Rui Jia, Juan S. Bonifacino
Summary: The ubiquitination of LC3B is reversed by the deubiquitinating enzyme USP10, thereby controlling the levels of LC3B and autophagic activity.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)