Article
Agricultural Engineering
Xiaowei Li, Rui Zhao, Shan Li, Yating Wang, Xinnan Wang, Wenya Yang, Mengchan Yang, Weihua Xiao, Shihui Yang, Xiaoqing Lin, Xiaojie Zheng, Xiaojun Ma, Ling Zhao, Wei Xiao, Limin Cao
Summary: This study focused on improving yeast strains for mixed sugar fermentation in lignocellulosic hydrolysates, with SPT2(OE) showing superior ethanol yield and titer within 24 hours in different hydrolysates.
INDUSTRIAL CROPS AND PRODUCTS
(2022)
Article
Engineering, Environmental
Carmen Padilla-Rascon, Juan Miguel Romero-Garcia, Encarnacion Ruiz, Inmaculada Romero, Eulogio Castro
Summary: The study evaluated an optimized process for obtaining furfural from olive stone, demonstrating its potential as a promising raw material for furfural production.
PROCESS SAFETY AND ENVIRONMENTAL PROTECTION
(2021)
Article
Engineering, Chemical
Elena Dominguez, Pablo G. del Rio, Aloia Romani, Gil Garrote, Lucilia Domingues
Summary: This study utilized Paulownia hardwood as an energy crop to convert hemicellulose into ethanol, successfully using engineered yeast and appropriate detoxification processes to produce ethanol. The results demonstrate the suitability of Paulownia biomass as a renewable source for ethanol production.
Article
Energy & Fuels
Lucia Coimbra, Karen Malan, Alejandra Fagundez, Mairan Guigou, Claudia Lareo, Belen Fernandez, Martin Pratto, Silvia Batista
Summary: Genetically engineered yeast strains were developed for efficient ethanol production from lignocellulosic biomass. The recombinant strain CAT-1-XIT (pRS42K::XI) showed promising results with 74% consumption of D-xylose and production of 12.6 g/L ethanol.
BIOENERGY RESEARCH
(2023)
Article
Green & Sustainable Science & Technology
Boyang He, Bo Hao, Haizhong Yu, Fen Tu, Xiaoyang Wei, Ke Xiong, Yajun Zeng, Hu Zeng, Peng Liu, Yuanyuan Tu, Yanting Wang, Heng Kang, Liangcai Peng, Tao Xia
Summary: This study demonstrates an effective yeast-engineering approach for efficient xylose utilization and provides a powerful strategy for enhancing bioethanol production in bioenergy crops.
Article
Biotechnology & Applied Microbiology
Paola Monteiro de Oliveira, Daria Aborneva, Nemailla Bonturi, Petri-Jaan Lahtvee
Summary: Lignocellulosic biomass is an attractive raw material for sustainable production of chemicals and materials using microbial cell factories. Non-conventional yeasts, such as R. toruloides CCT 7815, show promise in efficiently converting a wide range of substrates into different products, with high tolerance to inhibitors, making them suitable for biorefinery processes. In this study, different non-conventional yeast strains were screened on diluted hemicellulosic hydrolysate from Birch, and R. toruloides CCT 7815 demonstrated the best performance among all tested strains, showing high specific growth and substrate co-consumption rates under high hydrolysate conditions.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Jung-Hoon Bae, Mi-Jin Kim, Bong Hyun Sung, Yong-Su Jin, Jung-Hoon Sohn
Summary: The extracellular conversion of xylose to xylulose before uptake has been found to be effective in xylose fermentation or glucose/xylose co-fermentation, reducing the competition between glucose and xylose for cellular uptake. The engineered XI secretion system developed in this study may enable the simultaneous utilization of C5/C6 sugars from sustainable lignocellulosic biomass.
BIOTECHNOLOGY FOR BIOFUELS
(2021)
Article
Agricultural Engineering
Meilin Kong, Xiaowei Li, Tongtong Li, Xuebing Zhao, Mingjie Jin, Xin Zhou, Hanqi Gu, Vladimir Mrsa, Wei Xiao, Limin Cao
Summary: CCW12(OE) is a Saccharomyces cerevisiae strain constructed by overexpressing CCW12, which shows high ethanol production efficiency in various hydrolysates and exhibits better stress resistance and fermentation performance compared to the control strain WXY70.
BIORESOURCE TECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Yixuan Zhu, Jingtao Zhang, Lang Zhu, Zefang Jia, Qi Li, Wei Xiao, Limin Cao
Summary: Efficient xylose fermentation in budding yeast was achieved through rational promoter elements engineering, with HXT7 showing the best performance among surveyed promoters. The redox balance of the xylose utilization pathway was optimized to achieve a balanced xylose metabolism toward ethanol formation.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Lulu Liu, Mingjie Jin, Mingtao Huang, Yixuan Zhu, Wenjie Yuan, Yingqian Kang, Meilin Kong, Sajid Ali, Zefang Jia, Zhaoxian Xu, Wei Xiao, Limin Cao
Summary: The reported haploid Saccharomyces cerevisiae strain F106 has the ability to utilize xylose for ethanol production, with the XR-K270R mutant showing the best performance after a series of mutations. The study found that the yeast chromosomal copy number is positively correlated with increased ethanol production under the experimental conditions, indicating the potential for improving ethanol yield through genetic modifications.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Getachew Tafere Abrha, Qian Li, Xiaolin Kuang, Difan Xiao, Ellen Ayepa, Jinjian Wu, Huan Chen, Zhengyue Zhang, Yina Liu, Xiumei Yu, Quanju Xiang, Menggen Ma
Summary: This study obtained a yeast strain tolerant to furfural by overexpression and found that the overexpressing RDS1 strain exhibited faster recovery and shorter lag phase compared to its parental strain. The overexpressing RDS1 encoded a novel aldehyde reductase with high specific activity for furfural reduction, and it also showed improved reactive oxygen species resistance and cell wall response. The introduction of RDS1 overexpression promoted the expression level, which differentiated the tolerance phenotypes of RDS1 from the innate stress response of the parental strain. Overexpression of RDS1 involving diversified functional categories accounted for stress tolerance in yeast S. cerevisiae.
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
(2023)
Article
Biotechnology & Applied Microbiology
Andreea Cristina Dobrescu, Henrique Cesar Teixeira Veras, Cristiano Varrone, Jan Dines Knudsen
Summary: Propagating yeast on non-fermentable carbon sources can enhance xylose metabolism and improve efficiency in xylose fermentation.
FERMENTATION-BASEL
(2021)
Article
Agronomy
Heeyoung Park, Sang Un Park, Byeong-Kwan Jang, Jeong Jae Lee, Yong Suk Chung
Summary: Kenaf, an annual fiber crop mainly grown in India and China, is emerging as a promising bio-based energy source due to its rapid growth, CO2 absorption capabilities, and high productivity. The study evaluated 10 different cultivars of kenaf for cellulosic ethanol production, finding that ethanol yield and productivity were significantly impacted by cultivar type, rather than biomass composition. Engineering yeast to utilize xylose under pH control can maximize ethanol production from kenaf.
GLOBAL CHANGE BIOLOGY BIOENERGY
(2021)
Article
Energy & Fuels
Debora Danielle Virginio da Silva, Eduardo Machado, Otavio Danelussi, Miqueias Gomes dos Santos, Silvio Silverio da Silva, Kelly Johana Dussan
Summary: In this study, a repeated-batch fermentation of hemicellulosic sugarcane bagasse hydrolysate was conducted using two xylose-fermenting yeasts, Scheffersomyces stipitis and Scheffersomyces shehatae. The results showed that the fermentative parameters were significantly increased through the repeated-batch operation, and S. shehatae was 85% more efficient in xylose to ethanol conversion compared to S. stipitis.
BIOMASS CONVERSION AND BIOREFINERY
(2022)
Article
Engineering, Environmental
Rylan Cox, Vivek Narisetty, Eulogio Castro, Deepti Agrawal, Samuel Jacob, Gopalakrishnan Kumar, Deepak Kumar, Vinod Kumar
Summary: This study focused on the valorization of xylose derived from sugarcane bagasse and olive pits through hydrothermal and dilute acid pretreatment. The xylose-rich hydrolysate obtained was used for lactic acid (LA) production using a thermophilic Bacillus coagulans strain under non-sterile conditions. The maximum LA titers obtained were 97.8, 52.4, and 61.3 g/L with yields of 0.77, 0.66, and 0.71 g/g using pure xylose, xylose-rich sugarcane bagasse, and olive pit hydrolysates, respectively. A two-step aqueous two-phase system (ATPS) was employed for the recovery of LA from the xylose-rich stream, resulting in a recovery rate of 80-90%.
Article
Biotechnology & Applied Microbiology
Takahiro Yukawa, Takahiro Bamba, Mami Matsuda, Takanobu Yoshida, Kentaro Inokuma, Jungyeon Kim, Jae Won Lee, Yong-Su Jin, Akihiko Kondo, Tomohisa Hasunuma
Summary: Efficient production of 3,4-dihydroxybutyrate from xylose by engineered yeast was achieved through genetic and environmental perturbations. The biosynthetic pathway of 3,4-dihydroxybutyrate was established by deleting ADH6 and overexpressing yneI genes in yeast. Alkaline fermentation was performed to minimize xylonate accumulation and led to the highest combined product yields compared with previous studies.
BIOTECHNOLOGY AND BIOENGINEERING
(2023)
Article
Chemistry, Physical
Si Zhao, Zaosheng Lv, Yang Lei, Yanfen Huang
Summary: In this study, a composite superhydrophobic coating capable of applying to a variety of substrates was developed, which exhibited exceptional superhydrophobicity and self-cleaning properties for the efficient separation of oil/water mixtures. The reagents and instruments used in the procedure are easily available, suggesting the potential for large-scale production.
COLLOID AND POLYMER SCIENCE
(2023)
Article
Biotechnology & Applied Microbiology
Kentaro Inokuma, Shunya Miyamoto, Kohei Morinaga, Yuma Kobayashi, Ryota Kumokita, Takahiro Bamba, Yoichiro Ito, Akihiko Kondo, Tomohisa Hasunuma
Summary: In this study, a bioprocess was developed to produce 4-hydroxybenzoic acid (4-HBA) directly from cellulose using recombinant Pichia pastoris that displays heterologous cellulolytic enzymes on its cell surface. The cellulolytic enzymes, including beta-glucosidase, endoglucanase, and cellobiohydrolase, were co-displayed on the cell surface of P. pastoris. A highly 4-HBA-resistant chorismate pyruvate-lyase was also expressed in the cellulase-displaying strain. This strain achieved a 4-HBA yield of 975 mg/L from phosphoric acid swollen cellulose (PASC), which is over two times higher than the yield from glucose.
BIOTECHNOLOGY AND BIOENGINEERING
(2023)
Article
Chemistry, Multidisciplinary
Shangjie Zhang, Feng Guo, Qiao Yang, Yujia Jiang, Shihui Yang, Jiangfeng Ma, Fengxue Xin, Tomohisa Hasunuma, Akihiko Kondo, Wenming Zhang, Min Jiang
Summary: In this study, the oleaginous yeast Yarrowia lipolytica was rationally engineered to utilize methanol and produce value-added chemicals like succinic acid. Through metabolic pathway engineering and gene regulation, the recombinant strain showed enhanced methanol utilization and cell growth. Transcriptome analysis revealed the rebalancing of methylotrophic metabolism. The study demonstrates the successful biological conversion of methanol into valuable chemicals.
Article
Plant Sciences
Kenya Tanaka, Tomokazu Shirai, Christopher J. Vavricka, Mami Matsuda, Akihiko Kondo, Tomohisa Hasunuma
Summary: Quantitative metabolome dynamics reveal that high accumulation of glycolytic intermediates in darkness enable efficient metabolism switching for the smooth initiation of photosynthesis in cyanobacteria. Dark-to-light transitions in cyanobacteria involve drastic metabolic changes from dark respiratory metabolism to CO2 fixation through the Calvin-Benson-Bassham cycle. This study demonstrates rapid dynamic changes in metabolite concentrations and C-13 tracer incorporation after light irradiation in Synechocystis sp. PCC 6803, allowing estimation of nonstationary metabolic flux underlying CBB cycle activation. Accumulation of glycolytic intermediates is essential for efficient metabolism switching under fluctuating light environments, as indicated by the decrease in the initial oxygen evolution rate in mutants deficient in glycogen degradation or oxidative pentose phosphate pathways.
Article
Energy & Fuels
Daniel A. Alfaro-Sayes, Jerome Amoah, Nova Rachmadona, Shinji Hama, Tomohisa Hasunuma, Akihiko Kondo, Chiaki Ogino
Summary: The aim of this study was to evaluate the effect of alginate immobilization on the growth and lipid productivity of the microalgae Chlorella sorokiniana. The results showed that immobilization technology can enhance the growth and lipid productivity of microalgae.
JOURNAL OF PHYSICS-ENERGY
(2023)
Article
Microbiology
Yuji Haraguchi, Yuichi Kato, Kosuke Inabe, Akihiko Kondo, Tomohisa Hasunuma, Tatsuya Shimizu
Summary: Recently, a circular cell culture (CCC) system using microalgae and animal muscle cells was developed for sustainable food production. However, lactate accumulation from animal cells in the system posed a significant challenge. To address this issue, an advanced CCC system utilizing a lactate-assimilating cyanobacterium was employed. The cyanobacteria and animal cells exchanged substances via their waste media, leading to efficient amplification of animal muscle cells without the need for animal serum.
ARCHIVES OF MICROBIOLOGY
(2023)
Article
Multidisciplinary Sciences
Yuichi Kato, Kosuke Inabe, Yuji Haraguchi, Tatsuya Shimizu, Akihiko Kondo, Tomohisa Hasunuma
Summary: To develop a sustainable animal cell culture system, researchers introduced the lldD gene from Escherichia coli into Synechococcus sp. PCC 7002, enabling the strain to consume l-lactate. This consumption was accelerated by the expression of the lldP gene and an increase in culture temperature. The study provides new insight into the treatment of l-lactate by photosynthetic microorganisms, potentially enhancing the feasibility of animal cell culture industries.
SCIENTIFIC REPORTS
(2023)
Article
Biotechnology & Applied Microbiology
Erina Yoshida, Yuichi Kato, Akihiko Kanamoto, Akihiko Kondo, Tomohisa Hasunuma
Summary: This study analyzed the effect of nitrogen sources on the production of fucoxanthin by OPMS 30543X microalgae. It was found that using NaNO3 as the nitrogen source resulted in higher fucoxanthin content compared to using NH4Cl. This suggests that nitrogen sources can influence the metabolic processes and biosynthesis of fucoxanthin by OPMS 30543X. The findings of this study can guide the commercial production of fucoxanthin using microalgae.
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
(2023)
Article
Immunology
Atomu Yamaguchi, Noriaki Maeshige, Jiawei Yan, Xiaoqi Ma, Mikiko Uemura, Mami Matsuda, Yuya Nishimura, Tomohisa Hasunuma, Hiroyo Kondo, Hidemi Fujino, Zhi-Min Yuan
Summary: This study found that myotube-derived extracellular vesicles (EVs) can suppress macrophage inflammatory responses while activating the IRG1-itaconate pathway. The miRNA composition of EVs may be related to their anti-inflammatory effects.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Ryota Kumokita, Takanobu Yoshida, Tomokazu Shirai, Akihiko Kondo, Tomohisa Hasunuma
Summary: Aromatic secondary metabolites are widely used in various industries and their production currently relies on plant extraction. Microbe-based processes have recently attracted attention as sustainable alternatives. This study found that the yeast Pichia pastoris can produce higher levels of aromatic secondary metabolites from glycerol compared to glucose, and enhanced utilization of specific amino acids further increases the production.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Plant Sciences
Abigail Loren Tung Uy, Atsushi Yamamoto, Mami Matsuda, Toshihiro Arae, Tomohisa Hasunuma, Taku Demura, Misato Ohtani
Summary: Carbon flow shifts from glycolysis to the shikimate pathway during xylem vessel cell differentiation, accompanied by active changes in the levels of cellulose and lignin precursors.
PLANT AND CELL PHYSIOLOGY
(2023)
Article
Multidisciplinary Sciences
Mikiko Uemura, Noriaki Maeshige, Atomu Yamaguchi, Xiaoqi Ma, Mami Matsuda, Yuya Nishimura, Tomohisa Hasunuma, Taketo Inoue, Jiawei Yan, Ji Wang, Hiroyo Kondo, Hidemi Fujino
Summary: This study investigated the effect of electrical stimulation (ES) on the metabolism and inflammatory responses of macrophages. The results showed that ES had an anti-inflammatory effect by reducing inflammation and oxidative stress in macrophages, while increasing antioxidant metabolites. This suggests that ES can improve the function of macrophages.
SCIENTIFIC REPORTS
(2023)
Review
Biotechnology & Applied Microbiology
Shuai Zhao, Ting Zhang, Tomohisa Hasunuma, Akihiko Kondo, Xin-Qing Zhao, Jia-Xun Feng
Summary: This review compares the cellulase and xylanase biosynthesis in two filamentous fungi, P. oxalicum and T. reesei, and highlights the strikingly different modes of genome-wide regulation of these enzymes. The understanding of these differences may lead to improvements in fungal enzyme production through metabolic engineering and synthetic biology.
CRITICAL REVIEWS IN BIOTECHNOLOGY
(2023)
Meeting Abstract
Biotechnology & Applied Microbiology
Koichi Kitagawa, A. Ruhan, Kentaro Inokuma, Yuka Yoshigoe, Tomohisa Hasunuma, Toshiro Shirakawa
