Article
Hematology
Trine A. Kristiansen, Qinyu Zhang, Stefano Vergani, Elena Boldrin, Niklas Krausse, Oscar Andre, Pontus Nordenfelt, Mikael Sigvardsson, David Bryder, Jonas Ungerbaeck, Joan Yuan
Summary: The fetal-to-adult switch in hematopoietic stem cell behavior is characterized by changes in lineage output and entry into deep quiescence. The emergence of megakaryocyte-biased HSCs coincides with this developmental switch. Molecular changes in HSCs during the fetal-to-adult transition include acquisition of megakaryocyte lineage priming signatures and increased amplitude of early megakaryocyte differentiation events. LIN28B, which promotes fetal-like self-renewal, acts as an insulator against the establishment of a megakaryocyte-biased HSC pool. The developmental regulation of megakaryocyte priming may represent a switch for HSCs to prioritize self-renewal in the fetus and increased host protection in postnatal life.
Article
Biotechnology & Applied Microbiology
Bjorn Oback, Daniel A. Cossey
Summary: Animal breeding progresses mainly through the male germline, but this process responds slowly to environmental pressures. New breeding approaches using chimaeras can accelerate breeding and transmit elite male germlines. We propose a novel breeding platform that integrates embryo-based complementation, genomic selection, multiplication, and gene modification.
TRENDS IN BIOTECHNOLOGY
(2023)
Article
Cell & Tissue Engineering
Shuai Ma, Si Wang, Yanxia Ye, Jie Ren, Ruiqing Chen, Wei Li, Jiaming Li, Liyun Zhao, Qian Zhao, Guoqiang Sun, Ying Jing, Yuesheng Zuo, Muzhao Xiong, Yuanhan Yang, Qiaoran Wang, Jinghui Lei, Shuhui Sun, Xiao Long, Moshi Song, Shuyang Yu, Piu Chan, Jianwei Wang, Qi Zhou, Juan Carlos Izpisua Belmonte, Jing Qu, Weiqi Zhang, Guang-Hui Liu
Summary: This study provides a comprehensive framework to explore aging and rejuvenating trajectories at single-cell resolution, revealing cellular and molecular programs that instruct systemic revitalization by blood-borne factors. Specifically, it identified hematopoietic stem and progenitor cells (HSPCs) as one of the most responsive cell types to young blood exposure, initiating a continuum of cell state changes and restoring a youthful transcriptional regulatory program and cell-cell communications in HSPCs.
Review
Dentistry, Oral Surgery & Medicine
Jeeranan Manokawinchoke, Prasit Pavasant, Chalida Nakalekha Limjeerajarus, Nuttapol Limjeerajarus, Thanaphum Osathanon, Hiroshi Egusa
Summary: The studies indicate that cells perceive external stimuli through different mechanical stimulations and transduce them into internal signals via mechanoreceptors. Cell responses to mechanical stimuli depend on various factors, including the type, magnitude, and duration of the stimulation. Different mechanical stimulations lead to distinct cell responses based on factors like cell type and differentiation stage, ultimately modulating stem cell maintenance and differentiation.
ARCHIVES OF ORAL BIOLOGY
(2021)
Review
Medicine, Research & Experimental
Alexander C. Lewis, Lev M. Kats
Summary: The altered capacity for self-renewal and differentiation is a key feature of cancer, particularly in diseases like acute myeloid leukemia. Treatments are shifting towards targeting cancer stem cells and altering cell fate decisions to combat the disease.
EMBO MOLECULAR MEDICINE
(2021)
Article
Multidisciplinary Sciences
Gintautas Vainorius, Maria Novatchkova, Georg Michlits, Juliane Christina Baar, Cecilia Raupach, Joonsun Lee, Ramesh Yelagandula, Marius Wernig, Ulrich Elling
Summary: In this study, the authors show that cells can take two mechanistically distinct paths in the same transition paradigm when driven by the similar proneural factors Ascl1 and Ngn2. Ascl1 dismantles the pluripotency network and installs neuronal and trophoblast cell fates, while Ngn2 generates a neural stem cell-like intermediate supported by incomplete shutdown of the pluripotency network.
NATURE COMMUNICATIONS
(2023)
Review
Cell & Tissue Engineering
Cong Zhao, Wen Xie, Hecheng Zhu, Ming Zhao, Weidong Liu, Zhaoping Wu, Lei Wang, Bin Zhu, Shasha Li, Yao Zhou, Xingjun Jiang, Qiang Xu, Caiping Ren
Summary: Stem cells are unique cells with the ability to self-renew and differentiate into various functional cells. Recent studies have revealed the crucial roles of long non-coding RNAs (lncRNAs) and RNA-binding proteins (RBPs) in the self-renewal and differentiation of stem cells. This review summarizes the functions of lncRNAs and their RBPs in determining the fate of stem cells, focusing on their involvement in epigenetic regulation, signaling pathway regulation, splicing, mRNA stability, and subcellular localization. Understanding the regulatory mechanisms of lncRNAs and RBPs will contribute to the development of clinical regenerative medicine.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Cell & Tissue Engineering
Se-Ra Park, Seong-Kwan Kim, Soo-Rim Kim, Wook-Joon Yu, Seung-Jin Lee, Hwa-Yong Lee
Summary: Smoking has harmful effects on female fertility by inhibiting the functions of resident endometrial stem cells. Exposure to cigarette smoke extracts significantly suppresses various tissue regeneration-associated functions of endometrial stem cells by activating the SERPINB2 gene. Knockdown of SERPINB2 effectively abolishes the inhibitory effects of cigarette smoke on endometrial stem cell functions.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Cell & Tissue Engineering
Shenae L. Cafe, David A. Skerrett-Byrne, Camila Salum De Oliveira, Brett Nixon, Melissa J. Oatley, Jon M. Oatley, Tessa Lord
Summary: The nucleosome remodeling protein CHD4 plays a key role in regulating SSC function, with its loss impairing SSC regenerative capacity. CHD4 affects gene expression in spermatogonia not only through its association with the remodeling complex NuRD but also via interaction with the GDNF-responsive transcription factor SALL4, influencing fate decisions and maintenance in the spermatogonial pool.
Article
Cell & Tissue Engineering
Yuan Liu, Meimei Huang, Xiaodan Wang, Zinan Liu, Siqi Li, Ye-Guang Chen
Summary: This study reveals the critical role of methyltransferase-like 3 (Mettl3) in maintaining intestinal stem cell (ISC) stemness through N6-methyladenosine (m6A) methylation, independent of cell proliferation. The researchers identified four m6A-modified transcription factors that can restore stemness gene expression in Mettl3-deficient organoids, and also discerned 23 genes unrelated to cell proliferation. These findings demonstrate the importance of m6A modification in sustaining ISC stemness and its uncoupling from cell proliferation.
Review
Cell Biology
Feng Li, Ying Ye, Xiaohua Lei, Wensheng Zhang
Summary: With the development of science and technology, mankind's exploration of outer space has increased tremendously. Settling in outer space or on other planets could help solve the Earth's resource crisis, but such settlement will first face the problem of reproduction. There are considerable differences between outer space and the Earth's environment, with the effects of gravity being one of the most significant. Studying the possible effects and underlying mechanisms of microgravity on embryonic stem cell (ESC) differentiation and embryonic development could help provide solutions to healthy living and reproduction in deep space. This article summarizes recent research progress on the effects of microgravity on ESCs and early embryonic development and proposes hypotheses regarding the potential mechanisms. In addition, we discuss the controversies and key questions in the field and indicate directions for future research.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Cell & Tissue Engineering
Xueyue Wang, Yan Ruan, Junlei Zhang, Yanping Tian, Lianlian Liu, JiaLi Wang, Gaoke Liu, Yuda Cheng, Yixiao Xu, Yi Yang, Meng Yu, Binyu Zhao, Yue Zhang, Jiaqi Wang, Jiangjun Wang, Wei Wu, Ping He, Lan Xiao, Jiaxiang Xiong, Rui Jian
Summary: The study identifies two Yap splicing isoforms in ESCs with different distributions and effects on self-renewal and differentiation potential, with Yap472 playing a more essential role in neuroectoderm differentiation.
Article
Genetics & Heredity
Shiyu An, Dan Yao, Wenyi Zhang, Hao Sun, Tianyi Yu, Ruizhe Jia, Yang Yang
Summary: This study discovered the involvement of WDR36 in self-renewal and differentiation potential of human EPS cells, with the effects being reversible by inhibiting P53.
FRONTIERS IN GENETICS
(2022)
Article
Materials Science, Biomaterials
Yujie Zhang, Murielle Remy, Evgeny Apartsin, Emilie Prouve, Cecile Feuillie, Christine Labrugere, Nithavong Cam, Marie-Christine Durrieu
Summary: Ideal bone tissue engineering aims to promote bone regeneration by using biomaterial scaffolds, bone progenitor cells, and bone-forming factors. Biomimetic scaffolds mimic the native extracellular matrix and are used to study cell-ECM interactions. In this study, a unique combination of bioactive micro/nanopatterns on a polymer surface was hypothesized to enhance the differentiation of human mesenchymal stem cells.
BIOMATERIALS SCIENCE
(2023)
Article
Cell Biology
Faxiang Xu, Chunhao Deng, Zhili Ren, Liangyu Sun, Ya Meng, Weiwei Liu, Jianbo Wan, Guokai Chen
Summary: Exogenous lipids such as LPA can influence the metabolism of pluripotent stem cells and induce distinct phenotypes and transcriptome profiles in the cells, altering their primed state.
Article
Materials Science, Multidisciplinary
Claudio Conci, Emanuela Jacchetti, Laura Sironi, Lorenzo Gentili, Giulio Cerullo, Roberto Osellame, Giuseppe Chirico, Manuela Teresa Raimondi
Summary: The current methods for assessing biomaterial biocompatibility are outdated and unethical, and there is a need for a real-time research method. This study develops and validates the Microatlas window, providing a chicken embryo experimental model for biomaterial biocompatibility assessment and microscope field repositioning.
ADVANCED OPTICAL MATERIALS
(2022)
Article
Cell Biology
Maria Garofalo, Cecilia Pandini, Matteo Bordoni, Emanuela Jacchetti, Luca Diamanti, Stephana Carelli, Manuela Teresa Raimondi, Daisy Sproviero, Valeria Crippa, Serena Carra, Angelo Poletti, Orietta Pansarasa, Stella Gagliardi, Cristina Cereda
Summary: This study conducted gene expression analysis on sporadic ALS patients and healthy controls, and found that patients with high and low levels of nSOD1 have different gene expression patterns. High-nSOD1 patients activate pathways related to upregulation of HSP70 molecular chaperones, leading to reduced DNA damage under oxidative stress conditions.
Article
Biochemistry & Molecular Biology
Eveljn Scarian, Matteo Bordoni, Valentina Fantini, Emanuela Jacchetti, Manuela Teresa Raimondi, Luca Diamanti, Stephana Carelli, Cristina Cereda, Orietta Pansarasa
Summary: This study demonstrates the robustness of 3D cultures for the differentiation of stem cells in studying ALS, using bioprinting and iPSCs. The results show that the hydrogel-based constructs do not interfere with the differentiation process and electrophysiological features of the cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Multidisciplinary Sciences
Isabel M. Martin, Michele M. Nava, Sara A. Wickstroem, Frauke Graeter
Summary: This study investigates the mechanotransducing functions of the essential adhesion component integrin-linked kinase (ILK) using force-probe molecular-dynamics simulations. The results suggest that ATP plays a role in facilitating cellular force generation and migration.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Cell Biology
Clara Alice Musi, Luca Colnaghi, Arianna Giani, Erica Cecilia Priori, Giacomo Marchini, Matteo Tironi, Claudio Conci, Giulio Cerullo, Roberto Osellame, Manuela Teresa Raimondi, Andrea Remuzzi, Tiziana Borsello
Summary: The complexity of the human brain has so far hindered the development of in vitro models. However, using the Nichoid microscaffold device to culture brain cells and generate brain micro-tissues provides a new tool to study brain cell interactions and morphology.
Article
Engineering, Biomedical
Francesca Donnaloja, Manuela Teresa Raimondi, Letizia Messa, Bianca Barzaghini, Federica Carnevali, Emanuele Colombo, Davide Mazza, Chiara Martinelli, Lucia Boeri, Federica Rey, Cristina Cereda, Roberto Osellame, Giulio Cerullo, Stephana Carelli, Monica Soncini, Emanuela Jacchetti
Summary: Mechanical stimuli from the extracellular environment affect cell morphology and functionality. The use of a custom-made 3D microscaffold, called the Nichoid, for cultivating mesenchymal stem cells (MSCs) has been shown to enhance the expression of stemness markers. This device appears to regulate gene activity by altering intracellular force transmission. The findings suggest that the Nichoid can influence MSC behavior and gene regulation through mechanical manipulation.
APL BIOENGINEERING
(2023)
Proceedings Paper
Engineering, Biomedical
Claudio Conci, Emanuela Jacchetti, Laura Sironi, Lorenzo Gentili, Giulio Cerullo, Rebeca Martinez, Roberto Osellame, Mario Marini, Margaux Bouzin, Maddalena Collini, Laura D'Alfonso, Elmina Kabouraki, Maria Farsari, Anthi Ranella, Nikos Kehagias, Giuseppe Chirico, Manuela T. Raimondi
Summary: The current protocols for assessing the biocompatibility of biomaterials require sacrificing a large number of laboratory animals, which is ethically burdensome and costly. Intravital microscopy allows real-time study of implantation outcomes, but has limited capabilities for quantification in longitudinal studies. We present a novel chip that enables longitudinal studies of biomaterial implant reactions, and validate its potential for tissue regeneration using fluorescence microscopy in living chicken embryos.
BIOMEDICAL SPECTROSCOPY, MICROSCOPY, AND IMAGING II
(2022)
Meeting Abstract
Cell & Tissue Engineering
Letizia Messa, Bianca Barzaghini, Federica Rey, Cecilia Pandini, Gian Vincenzo Zuccotti, Cristina Cereda, Stephana Carelli, Manuela Teresa Raimondi
TISSUE ENGINEERING PART A
(2022)
Meeting Abstract
Cell & Tissue Engineering
Alessandra Nardini, Claudio Conci, Emanuela Jacchetti, Giulio Cerullo, Rebeca Martinez Vazquez, Roberto Osellame, Gianni Ciofani, Manuela Teresa Raimondi
TISSUE ENGINEERING PART A
(2022)
Meeting Abstract
Cell & Tissue Engineering
Valentina Parodi, Emanuela Jacchetti, Arianna Bresci, Benedetta Talone, Roberto Osellame, Giulio Cerullo, Dario Polli, Manuela Teresa Raimondi
TISSUE ENGINEERING PART A
(2022)
Meeting Abstract
Cell & Tissue Engineering
Simone Perottoni, Nuno Neto, Cesare Di Nitto, Michael Monaghan, Manuela Teresa Raimondi
TISSUE ENGINEERING PART A
(2022)
Meeting Abstract
Cell & Tissue Engineering
Simone Perottoni, Nuno G. Neto, Cesare di Nitto, Ruslan Dmitriev, Manuela T. Raimondi, Michael G. Monaghan
TISSUE ENGINEERING PART A
(2022)
Meeting Abstract
Cell & Tissue Engineering
Manuela T. Raimondi
TISSUE ENGINEERING PART A
(2022)
Meeting Abstract
Cell & Tissue Engineering
Carolina Testa, Stefania Oliveto, Francesca Donnaloja, Pietro Pinoli, Emanuela Jacchetti, Roberto Osellame, Giulio Cerullo, Stefano Biffo, Stefano Ceri, Manuela Teresa Raimondi
TISSUE ENGINEERING PART A
(2022)
Meeting Abstract
Cell & Tissue Engineering
Bianca Barzaghini, Federica Rey, Letizia Messa, Francesca Fanizza, Roberto Osellame, Giulio Cerullo, Gian Vincenzo Zuccotti, Stephana Carelli, Manuela Teresa Raimondi
TISSUE ENGINEERING PART A
(2022)