Letter
Oncology
Shrikant B. Mali
Summary: Intravital microscopy and optical coherency tomography are powerful imaging tools that have revolutionized cancer diagnosis and therapies. They allow for real-time observation of biological processes in vivo, providing insights into tumor behaviors. Recent developments in techniques for observing deep tissues have improved the detection of subcellular events and made these technologies indispensable in basic sciences and drug development.
Review
Immunology
Anika Gruneboom, Oliver Aust, Zuelal Cibir, Flora Weber, Dirk M. Hermann, Matthias Gunzer
Summary: Innate immune cells play a crucial role in defense against infections and sterile inflammation, with recent advances in microscopy providing insights into their biology. Neutrophils and macrophages have protective roles during tumor growth and ischemia-reperfusion damage, but can also exacerbate diseases. Future directions include enhancing knowledge about innate immune cells and their potential applications in disease diagnostics.
IMMUNOLOGICAL REVIEWS
(2022)
Article
Biochemical Research Methods
Ahmad Shirazi, Tayebeh Sahraeibelverdi, Miki Lee, Haijun Li, Joonyoung Yu, Sangeeta Jaiswal, Kenn R. Oldham, Thomas D. Wang
Summary: We demonstrate a side-view dual axes confocal endomicroscope that can be repetitively inserted in hollow organs of genetically engineered mice for in vivo real-time imaging in both horizontal and vertical planes. Near infrared (NIR) excitation at λex = 785 nm was used, and a monolithic 3-axis parametric resonance scan mirror fabricated using micro-electromechanical systems (MEMS) technology enabled post-objective scanning in the distal end of a 4.19 mm diameter instrument. Torsional and serpentine springs were designed to switch the mode of imaging between vertical and horizontal planes by tuning the actuation frequency. This system achieved real-time in-vivo images with a depth of 310 μm and lateral and axial resolution of 1.75 and 7.5 μm, respectively, in both horizontal and vertical planes, allowing identification of individual cells and discrete mucosal structures.
BIOMEDICAL OPTICS EXPRESS
(2023)
Article
Engineering, Electrical & Electronic
Mitchell Kuss, Ayrianne J. Crawford, Olawale A. Alimi, Michael A. Hollingsworth, Bin Duan
Summary: The development of imaging windows and microscope mounts for longitudinal observation of internal tissues in living mice enhances the understanding of disease progression and cellular changes.
Article
Biotechnology & Applied Microbiology
Andreas Boden, Francesca Pennacchietti, Giovanna Coceano, Martina Damenti, Michael Ratz, Ilaria Testa
Summary: A new 3D pRESOLFT microscope method with sub-80-nm resolution has been developed to visualize the volumetric architecture of organelles and molecules inside whole living cells, allowing for targeted 3D confinement of fluorescence and observation of dynamic structural alterations in cells.
NATURE BIOTECHNOLOGY
(2021)
Article
Chemistry, Analytical
Cory Juntunen, Isabel M. Woller, Yongjin Sung
Summary: Hyperspectral 3D imaging integrates SPOT and FTS technologies for acquiring projection images from different viewing angles instantly and performing high-spectral-resolution imaging, which is validated through imaging performance using fluorescent beads and sunflower pollens.
Article
Optics
Jakob G. P. Lingg, Thomas S. Bischof, Bernardo A. Arus, Emily D. Cosco, Ellen M. Sletten, Christopher J. Rowlands, Oliver T. Bruns, Andriy Chmyrov
Summary: The development of fluorophores with photoemission beyond 1000 nm provides the opportunity to develop novel fluorescence microscopes sensitive to those wavelengths. Imaging at wavelengths beyond the visible spectrum enables imaging depths of hundreds of microns in intact tissue, making this attractive for volumetric imaging applications.
LASER & PHOTONICS REVIEWS
(2023)
Article
Nanoscience & Nanotechnology
Yuan Luo, Ming Lun Tseng, Sunil Vyas, Ting-Yu Hsieh, Jui-Ching Wu, Shang-Yang Chen, Hsiao-Fang Peng, Vin-Cent Su, Tzu-Ting Huang, Hsin Yu Kuo, Cheng Hung Chu, Mu Ku Chen, Jia-Wern Chen, Yu-Chun Chen, Kuang-Yuh Huang, Chieh-Hsiung Kuan, Xu Shi, Hiroaki Misawa, Din Ping Tsai
Summary: Light-sheet fluorescent microscopy (LSFM) is the leading technique for in vivo imaging in the fields of disease, medicine, and cell biology research. This study demonstrates the successful integration of a nanophotonic meta-lens as the illumination component for LSFM, addressing the challenges of high image resolution and optical sectioning. With the meta-lens, the LSFM system complexity is significantly reduced, enabling multicolor fluorescent imaging of live biological specimens with cellular resolution.
Article
Developmental Biology
Juan Eduardo Rodriguez-Gatica, Vira Iefremova, Liubov Sokhranyaeva, Si Wah Christina Au Yeung, Yannik Breitkreuz, Oliver Brustle, Martin Karl Schwarz, Ulrich Kubitscheck
Summary: Organoids, derived from stem cells, provide a new approach to model human development and disease. By using light-sheet fluorescence expansion microscopy, cellular and subcellular structural details of organoids can be observed in three dimensions, allowing for qualitative and quantitative assessment in developmental and disease-related studies.
Article
Urology & Nephrology
Jesus Z. Villarreal, Javiera Perez-Anker, Susana Puig, Marc Xipell, Gerard Espinosa, Esther Barnadas, Ana B. Larque, J. Malvehy, Ricard Cervera, Arturo Pereira, Antonio Martinez-Pozo, Luis F. Quintana, Adriana Garcia-Herrera
Summary: This study demonstrates the feasibility of using ex vivo confocal microscopy for the analysis of basic patterns of lesion in nephropathology. FuCM proves to be as effective as conventional techniques in evaluating acute tubular necrosis and interstitial fibrosis changes, facilitating rapid evaluation of kidney biopsies and restructuring the clinical workflow in renal histopathology.
CLINICAL KIDNEY JOURNAL
(2023)
Article
Multidisciplinary Sciences
Alice Sandmeyer, Lili Wang, Wolfgang Huebner, Marcel Mueller, Benjamin K. Chen, Thomas Huser
Summary: Cost-effective, highly portable, and easy to use high-resolution live-cell imaging systems can revolutionize research in challenging environments, enabling real-time tracking of virus particles and studying infection mechanisms.
Article
Optics
Chang-Soo Kim, Junyoung Kim, Hongki Yoo
Summary: The high-speed color 3D imaging method based on patterned illumination with a negative pinhole array overcomes the limitations of traditional pinhole arrays for brighter color images. Additionally, the proposed 3D image processing algorithm using 2D cross-correlation and multiple filtering masks significantly enhances the lateral resolution of color acquisition. This high-speed color 3D measurement technology with a negative pinhole array is demonstrated to be a useful tool for rapid and accurate non-contact measurements in various fields.
Article
Oncology
Samuel Nussbaum, Mira Shoukry, Mohammed Ali Ashary, Ali Abbaszadeh Kasbi, Mizba Baksh, Emmanuel Gabriel
Summary: This review discusses recent advances in cancer imaging, including artificial intelligence, molecular imaging, and intravital imaging. These technologies have the potential to improve the detection, monitoring, and treatment of cancer.
Review
Chemistry, Multidisciplinary
Norraseth Kaeokhamloed, Samuel Legeay, Emilie Roger
Summary: Advanced drug delivery system using nanocarriers is a major application of nanotechnology in pharmacotherapeutics. However, the clinical translation of nanomedicine faces challenges, and this review focuses on utilizing Fo•rster resonance energy transfer (FRET) as a method to track intact nanocarriers in vivo for studying pharmacokinetics.
JOURNAL OF CONTROLLED RELEASE
(2022)
Article
Urology & Nephrology
Jesus Z. Villarreal, J. Perez-Anker, S. Puig, G. Pellacani, M. Sole, J. Malvehy, Luis F. Quintana, A. Garcia-Herrera
Summary: This study evaluated the feasibility of using ex vivo confocal microscopy in non-neoplastic kidney biopsies, and found that confocal images correlated well with conventional histological sections without introducing artifacts to the samples. The ease and speed of image acquisition, along with the quality of digitally stained images obtained, suggest promise for clinical applications in nephrology and renal transplantation.
JOURNAL OF NEPHROLOGY
(2021)
Article
Engineering, Electrical & Electronic
Simon Lorenzo, Olav Solgaard
Summary: We have developed a wavelength-multiplexed system of optical fiber-based photonic-crystal microphones for acoustic source localization. The system is capable of accurately localizing sound sources in the environment with a wide bandwidth and high sensitivity.
IEEE SENSORS JOURNAL
(2022)
Article
Physics, Applied
Kenneth J. Leedle, Uwe Niedermayer, Eric Skaer, Karel Urbanek, Yu Miao, Payton Broaddus, Olav Solgaard, Robert L. Byer
Summary: This study presents two compact designs of ultrafast electron injectors with integrated focusing, which offer high brightness electron beams. These electron sources are suitable for various applications, including dielectric laser accelerator experiments and ultrafast electron diffraction.
JOURNAL OF APPLIED PHYSICS
(2022)
Article
Nanoscience & Nanotechnology
Nina Vaidya, Olav Solgaard
Summary: This article introduces the use of immersion optics to create systems with improved optical concentration and coupling. The immersion graded index optical concentrators, which do not require tracking of the light source, are described through theory, simulations, and experiments. A generalized design guide equation is presented to create various immersion graded index optics based on concentration, refractive index, height, and efficiency requirements. Glass and polymer fabrication techniques are also introduced for creating broadband transparent graded index materials with large refractive index ranges. Prototype devices demonstrate 3x optical concentration with over 90% efficiency.
MICROSYSTEMS & NANOENGINEERING
(2022)
Editorial Material
Surgery
Sibel Emir Diltemiz, Yavuz Nuri Ertas, Christopher H. Contag, Nureddin Ashammakhi
JOURNAL OF CRANIOFACIAL SURGERY
(2023)
Article
Chemistry, Analytical
Sandra Nicole Manosalvas-Kjono, Ronald Quan, Olav Solgaard
Summary: This study introduces an MEMS amplitude and phase monitor that can simultaneously sense and drive the movement of micro-actuators, providing real-time and accurate position monitoring.
Review
Biotechnology & Applied Microbiology
Nasrullah Jan, Asadullah Madni, Safiullah Khan, Hassan Shah, Faizan Akram, Arshad Khan, Derya Ertas, Mohammad F. Bostanudin, Christopher H. Contag, Nureddin Ashammakhi, Yavuz Nuri Ertas
Summary: This article discusses the preparation methods and applications of cell membrane-coated PLGA nanoparticles, as well as their role in cancer therapy, inflammation management, cardiovascular disease treatment, and infection control. It also highlights the challenges and future research directions of cell membrane-camouflaged nanoparticles.
BIOENGINEERING & TRANSLATIONAL MEDICINE
(2023)
Article
Biophysics
Alexander Farnum, Michael Parnas, Ehsanul Hoque Apu, Elyssa Cox, Noel Lefevre, Christopher H. Contag, Debajit Saha
Summary: There is evidence that cancer alters cellular metabolic processes, which can be detected through the volatile organic compound compositions emitted by cancer cells. This study developed an insect olfactory neural circuit-based VOC sensor to detect oral cancer cells. The results showed that this method can effectively distinguish different oral cancer cell lines from each other and non-cancer cell lines.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Ecology
Sean. M. M. Sultaire, Yuki Kawai-Harada, Ashley Kimmel, Emily. M. M. Greeson, Patrick. J. J. Jackson, Christopher. H. H. Contag, Carl. W. W. Lackey, Jon. P. P. Beckmann, Joshua. J. J. Millspaugh, Robert. A. A. Montgomery
Summary: In the first two decades of the 21st century, the population of American black bear (Ursus americanus) has rebounded and expanded its range into previously extinct areas. Habitat quality and availability are crucial factors limiting the range expansion, especially in western Nevada, USA. Through various data collection methods, including cameras, hair sampling, genotyping, and GPS tracking, we found that black bears preferred the Sierra Nevada with conifer land cover over the Great Basin Desert. The population density of black bears in the Sierra Nevada was over 4 times higher than in desert mountain ranges, indicating limited range expansion into the western Great Basin due to habitat quality and availability.
JOURNAL OF WILDLIFE MANAGEMENT
(2023)
Article
Chemistry, Analytical
Amina Gharbi, Ahmed Yahia Kallel, Olfa Kanoun, Wissem Cheikhrouhou-Koubaa, Christopher H. H. Contag, Iulian Antoniac, Nabil Derbel, Nureddin Ashammakhi
Summary: Monitoring changes in intracranial pressure caused by edema is crucial for improving outcomes in trauma or surgery. Implantable sensors have been explored, but the risk of brain tissue injury during their removal remains. In this study, we demonstrate a bioactive glass-based hydration sensor that can effectively monitor brain edema and provide an alert for increased intracranial pressure.
Article
Nanoscience & Nanotechnology
Sunil Pai, Carson Valdez, Taewon Park, Maziyar Milanizadeh, Francesco Morichetti, Andrea Melloni, Shanhui Fan, Olav Solgaard, David A. B. Miller
Summary: Programmable feedforward photonic meshes of Mach-Zehnder interferometers are computational optical circuits that have many classical and quantum computing applications. A new architecture capable of power monitoring any waveguide segment in a feedforward photonic circuit is designed and experimentally verified. The scheme is experimentally realized in a 6 x 6 triangular mesh silicon photonic chip and can resolve optical powers in a 3 x 3 triangular mesh based on response measurements in only two output detectors.
Article
Materials Science, Biomaterials
Chima V. Maduka, Mohammed Alhaj, Evran Ural, Maxwell M. Kuhnert, Oluwatosin M. Habeeb, Anthony L. Schilmiller, Kurt D. Hankenson, Stuart B. Goodman, Ramani Narayan, Christopher H. Contag
Summary: The stereochemistry of polylactide (PLA) affects its thermal and physicochemical properties, including degradation profiles. However, the role of PLA stereochemistry and its immune responses in vivo and in vitro remains unclear. In this study, a bioenergetic model was used to investigate the immune cellular responses to different PLA stereochemistries. The findings provide mechanistic explanations for the diverse immune responses to PLA and emphasize the importance of immunometabolism in the biocompatibility of biomaterials used in medicine.
ACS BIOMATERIALS SCIENCE & ENGINEERING
(2023)
Review
Chemistry, Multidisciplinary
Mustafa Nakipoglu, Aysen Tezcaner, Christopher H. Contag, Nasim Annabi, Nureddin Ashammakhi
Summary: Bioadhesives with antimicrobial properties enable easier and safer treatment of wounds. However, designing these biomaterials with the required properties can be challenging. Developing bioadhesives with tunable physical, chemical, and biological properties is crucial for future advancements.
ADVANCED MATERIALS
(2023)
Article
Multidisciplinary Sciences
Sunil Pai, Zhanghao Sun, Tyler W. Hughes, Taewon Park, Ben Bartlett, Ian A. D. Williamson, Momchil Minkov, Maziyar Milanizadeh, Nathnael Abebe, Francesco Morichetti, Andrea Melloni, Shanhui Fan, Olav Solgaard, David A. B. Miller
Summary: Integrated photonic neural networks offer a promising and energy-efficient platform for machine learning with wide-ranging scientific and commercial applications. By combining Mach-Zehnder interferometer mesh networks with nonlinearities, photonic neural networks efficiently transform optically encoded inputs. We experimentally trained a three-layer, four-port silicon photonic neural network using programmable phase shifters and optical power monitoring, achieving high accuracy comparable to digital simulations in image recognition tasks.
Article
Optics
Sunil Pai, Taewon Park, Marshall Ball, Bogdan Penkovsky, Michael Dubrovsky, Nathnael Abebe, Maziyar Milanizadeh, Francesco Morichetti, Andrea Melloni, Shanhui Fan, Olav Solgaard
Summary: With the increasing popularity of blockchain technology and cryptocurrency, photonic computing has emerged as an efficient hardware platform to reduce the energy costs of verifying transactions in decentralized cryptonetworks. To mitigate the sensitivity to photonic hardware errors, the proposed LightHash cryptographic scheme implements robust and low-bit precision matrix multiplication in programmable silicon photonic networks. The scheme also includes an error mitigation scheme and simulations to analyze energy-efficiency trade-offs. This hardware solution provides an error-resistant and efficient option for decentralized photonic blockchains.
Article
Chemistry, Multidisciplinary
Ashley Makela, Melissa A. Schott, Olivia C. Sehl, Julia J. Gevaert, Paula J. Foster, Christopher H. Contag
Summary: The use of imaging techniques to detect and monitor the movement and accumulation of tumor cells can provide valuable insights for understanding cancer metastasis and guiding therapeutic development. Magnetic Particle Imaging (MPI) is an emerging imaging technique that allows for specific, quantitative and sensitive detection of iron-labeled cells. Combining multiple imaging modalities such as MPI, in vivo bioluminescent imaging (BLI), and computed tomography (CT) provides information on immune escape, metastasis, and cell accumulation over time, helping to understand cancer progression and develop targeted therapies.
NANOSCALE ADVANCES
(2022)
