Article
Veterinary Sciences
Elena De Angelis, Roberta Saleri, Paolo Martelli, Lisa Elviri, Annalisa Bianchera, Carlo Bergonzi, Marta Pirola, Roberta Romeo, Melania Andrani, Valeria Cavalli, Virna Conti, Ruggero Bettini, Benedetta Passeri, Francesca Ravanetti, Paolo Borghetti
Summary: The study demonstrates that 3D printed CH scaffolds have good porosity for colonization and synthetic activity of chondrocytes. Enrichment with hyaluronic acid provides a more stable chondrocyte phenotype. The use of 3D CH scaffolds with PL instead of FBS in the medium results in increased gene expression of relevant ECM components.
FRONTIERS IN VETERINARY SCIENCE
(2021)
Article
Biotechnology & Applied Microbiology
Nadine Frerker, Tommy A. A. Karlsen, Maria Stensland, Tuula A. A. Nyman, Simon Rayner, Jan E. E. Brinchmann
Summary: Focal lesions of articular cartilage can cause pain and reduced joint function, leading to osteoarthritis if untreated. In vitro generated, scaffold-free autologous cartilage discs made from articular chondrocytes were compared to those made from bone marrow-derived mesenchymal stromal cells. Articular chondrocytes produced more extracellular matrix and contained more articular cartilage proteins, while mesenchymal stromal cells had more proteins associated with cartilage hypertrophy and bone formation. MicroRNA analysis suggested that differential expression of microRNAs played a key role in the differential synthesis of proteins. Therefore, articular chondrocytes are preferred for engineering articular cartilage.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Review
Biotechnology & Applied Microbiology
Xia Zhao, Daniel A. Hu, Di Wu, Fang He, Hao Wang, Linjuan Huang, Deyao Shi, Qing Liu, Na Ni, Mikhail Pakvasa, Yongtao Zhang, Kai Fu, Kevin H. Qin, Alexander J. Li, Ofir Hagag, Eric J. Wang, Maya Sabharwal, William Wagstaff, Russell R. Reid, Michael J. Lee, Jennifer Moriatis Wolf, Mostafa El Dafrawy, Kelly Hynes, Jason Strelzow, Sherwin H. Ho, Tong-Chuan He, Aravind Athiviraham
Summary: Cartilage, especially articular cartilage, is crucial for joint function and mobility, but has limited self-repair capacity. Cartilage tissue engineering (CTE) holds promise for repair, requiring effective chondrogenic factors, ample chondrogenic progenitors, and cell-friendly, biocompatible scaffold materials for success. Progress in these areas, along with advancements in 3D bio-printing, may improve clinical outcomes of cartilage repair.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
R. Olmos-Juste, G. Larranaga-Jaurrieta, I. Larraza, S. Ramos-Diez, S. Camarero-Espinosa, N. Gabilondo, A. Eceiza
Summary: In this study, functional alginate and waterborne polyurethane scaffolds were developed for articular cartilage regeneration using 3D bioprinting technology. It was found that scaffolds with a higher alginate content showed better 3D printing performance, structural integrity, and cell viability. After 28 days of experiments, these scaffolds were capable of maintaining cell numbers and synthesizing glycosaminoglycans, indicating their potential application for in vitro regeneration of articular cartilage tissue.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Nanoscience & Nanotechnology
Zhiyao Liao, Liwei Fu, Pinxue Li, Jiang Wu, Xun Yuan, Chao Ning, Zhengang Ding, Xiang Sui, Shuyun Liu, Quanyi Guo
Summary: A bioactive multifunctional scaffold was developed using Mg2+ and aptamer Apt19S to promote articular cartilage regeneration and early inflammatory regulation, offering a promising strategy for AC defect treatment.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Review
Engineering, Biomedical
Wenying Wei, Honglian Dai
Summary: Osteochondral defect regeneration remains a challenging issue in the musculoskeletal system, with traditional clinical treatments showing limited efficacy. However, the development of tissue engineering has provided more promising results in regenerating damaged osteochondral tissues.
BIOACTIVE MATERIALS
(2021)
Review
Biotechnology & Applied Microbiology
Xiaoshen Hu, Weiyang Zhang, Xiang Li, Dongling Zhong, Yuxi Li, Juan Li, Rongjiang Jin
Summary: Studies have shown that chondrocytes tend to dedifferentiate during cell amplification, losing their physiological properties, but recent research has made progress in redifferentiation control. By modulating various factors in cell culture, similar physiological characteristics and functions to human cartilage tissue have been achieved, offering hope for future cartilage repair and treatment.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Materials Science, Multidisciplinary
Kai Yan, Xiao Zhang, Yang Liu, Jiangqi Cheng, Chenjun Zhai, Kai Shen, Wenwei Liang, Weimin Fan
Summary: In this study, a porous SF-HPCMA bioink capable of 3D bioprinting was prepared for the first time using silk fibroin (SF) and hydroxypropyl cellulose methacrylate (HPCMA). The SF-HPCMA scaffolds bioprinted using this bioink showed excellent mechanical properties and suitable porosity. In vitro experiments demonstrated that the SF-HPCMA scaffolds were highly biocompatible and could effectively promote cell proliferation and chondrogenic differentiation, resulting in higher quality cartilage tissue. Implantation of SF-HPCMA scaffolds successfully repaired articular cartilage defects in rabbit knees.
MATERIALS & DESIGN
(2023)
Article
Biotechnology & Applied Microbiology
Andre M. Souza Plath, Stephanie Huber, Serena R. R. Alfarano, Daniel F. F. Abbott, Minghan Hu, Victor Mougel, Lucio Isa, Stephen J. J. Ferguson
Summary: To improve the integration of osteoarthritis scaffold-based grafts, electrospun poly(& epsilon;-caprolactone)/zein-based scaffolds were proposed. The zein surface segregation in the membranes changed the hydrophobic behavior of the materials and increased the cell yield and metabolic activity. The co-electrospun PCL/zein scaffolds showed promising surface and biocompatibility properties for articular-tissue-engineering applications.
BIOENGINEERING-BASEL
(2023)
Review
Engineering, Biomedical
Kang Sun, Chao Tao, Dong-An Wang
Summary: Tissue engineered cartilaginous constructs have made significant advances in the past decades as a treatment for osteoarthritis. Scaffold-free constructs, which involve only cells and cell-derived matrix, have several advantages over scaffold-based constructs in terms of relying on cells to produce matrix, avoiding interference with cell signaling and introducing no exogenous impurities.
BIOMEDICAL MATERIALS
(2022)
Review
Engineering, Multidisciplinary
Liangbin Zhou, Peng Guo, Matteo D'Este, Wenxue Tong, Jiankun Xu, Hao Yao, Martin J. Stoddart, Gerjo J. V. M. van Osch, Kevin Ki-Wai Ho, Zhen Li, Ling Qin
Summary: Articular cartilage (AC) is a flexible connective tissue located on the bone surface in the joints. AC defects are common in physically active individuals. Functionalized hydrogels have emerged as promising substitutes for damaged cartilage in AC repair due to their favorable properties. This article introduces the composition, structure, and function of AC and its defects, and reviews the design and fabrication of functionalized hydrogels for AC repair.
Article
Orthopedics
Omar D. Subedar, Loraine L. Y. Chiu, Stephen D. Waldman
Summary: Various cell synchronization methods were tested on primary articular chondrocytes, with double-thymidine method showing the highest cell purity and significantly better glycosaminoglycan and collagen II amounts in tissue constructs. Initial synchronization led to long-term changes in cartilaginous tissue formation, possibly due to the rapid auto-induction of TGF-betas by actively dividing S phase cells.
Article
Engineering, Multidisciplinary
Xue Yang, Shuai Li, Ya Ren, Lei Qiang, Yihao Liu, Jinwu Wang, Kerong Dai
Summary: Three-dimensional printed hydrogel is a promising approach in cartilage tissue engineering due to its similarity to the extracellular matrix and its ability to fabricate 3D cell culture scaffolds. Various hydrogels, both natural and synthetic, have been tested for 3D printing in vitro articular cartilage tissues. The advancement in materials and printing techniques allows for the fabrication of delicate cartilage structures on multiple scales. Stimuli-responsive hydrogels and their application prospects in tissue engineering are also discussed in this review. The development of novel composite hydrogels that meet the requirements of native articular cartilage is crucial for further advancement in the field.
COMPOSITES PART B-ENGINEERING
(2022)
Article
Polymer Science
Liang Lu, Xifu Shang, Bin Liu, Weijian Chen, Yu Zhang, Shuyun Liu, Xiang Sui, Aiyuan Wang, Quanyi Guo
Summary: This paper explores the application value of tissue engineering in vitro for the construction of artificial cartilage. By seeding healthy porcine chondrocytes on articular cartilage extracellular matrix (ACECM) scaffolds, it was found that the cells performed well in terms of adhesion and growth on the scaffolds, and they secreted collagen type II. Moreover, the porcine ACECM scaffolds showed good biocompatibility and could be used for xenotransplantation without significant immune inflammatory response. Therefore, ACECM-oriented scaffold is an ideal natural biomaterial for cartilage tissue engineering.
Article
Chemistry, Multidisciplinary
Angeliki Dimaraki, Pedro J. Diaz-Payno, Michelle Minneboo, Mahdiyeh Nouri-Goushki, Maryam Hosseini, Nicole Kops, Roberto Narcisi, Mohammad J. Mirzaali, Gerjo J. V. M. van Osch, Lidy E. Fratila-Apachitei, Amir A. Zadpoor
Summary: The study aimed to bioprint a scaffold with different zonal cell densities to mimic the organization of articular cartilage. Live/dead analysis and histological examination showed the ability to generate zonal cell density with high viability and smooth transition between zones, with higher glycosaminoglycan deposition in the highest cell density zone. These findings pave the way towards bioprinting complex zonal cartilage scaffolds as single units, advancing the translation of cartilage tissue engineering into clinical practice.
APPLIED SCIENCES-BASEL
(2021)
Article
Orthopedics
David Grevenstein, Andreas Mamilos, Volker H. Schmitt, Tanja Niedermair, Willi Wagner, C. James Kirkpatrick, Christoph Brochhausen
Summary: The study analyzed histological and immunohistological findings from five patients who underwent spheroid-based autologous chondrocyte transplantation after traumatic lesions of the knee, showing excellent histological results in terms of hyaline articular cartilage regeneration. This indicates that spheroid-based autologous chondrocyte transplantation is a promising strategy for treating traumatic lesions of the knee's articular cartilage.
KNEE SURGERY SPORTS TRAUMATOLOGY ARTHROSCOPY
(2021)
Article
Dentistry, Oral Surgery & Medicine
Anna Maria Tanneberger, Sarah Al-Maawi, Carlos Herrera-Vizcaino, Anna Orlowska, Alica Kubesch, Robert Sader, C. J. Kirkpatrick, Shahram Ghanaati
Summary: The study characterized the cellular reaction to a xenogeneic resorbable collagen membrane of porcine origin in Wistar rats over 30 days. It was found that the membrane induced a large number of mononuclear cells, including lymphocytes, macrophages, and fibroblasts, as well as multinucleated giant cells (MNGCs) expressing specific markers. The significant increase in MNGC number correlated with a high rate of vascularization compared to the control group, suggesting a potential role for MNGCs in biomaterial degradation.
CLINICAL ORAL INVESTIGATIONS
(2021)
Article
Orthopedics
Michael J. Pflueger, Dara E. Froemel, Andrea Meurer
Summary: The study found that poor physical health condition is associated with negative perioperative outcomes in revision total hip arthroplasty, especially in patients with cardiac comorbidities.
JOURNAL OF ARTHROPLASTY
(2021)
Article
Biochemistry & Molecular Biology
Susana G. Guerreiro, Ronald E. Unger, Nuno M. F. S. A. Cerqueira, Anne Sartoris, Maria J. Martins, Mario A. Barbosa, Raquel Soares, Pedro L. Granja, Charles J. Kirkpatrick
Summary: The study found that cocultures of fibroblasts and human endothelial cells stimulate collagen synthesis and growth factors production by fibroblasts, ultimately affecting the formation and distribution of microvessel-like structures in cell cultures. Areas with activated fibroblasts and high alkaline phosphatase (ALP) activity were also observed in the cocultures, indicating the impact of ALP on collagen proteins' aggregation, cell migration, and microvessel assembly. These findings suggest that bioinformatics and coculture systems are valuable tools for investigating the involvement of proteins like collagen and ALP in angiogenesis.
JOURNAL OF CELLULAR BIOCHEMISTRY
(2021)
Article
Orthopedics
Marco Brenneis, Sebastian Braun, Stefan van Drongelen, Benjamin Fey, Timur Tarhan, Felix Stief, Andrea Meurer
Summary: This study compared the accuracy and reliability of two-dimensional and three-dimensional preoperative planning for total hip arthroplasty, with a focus on stem morphology. The results suggest that three-dimensional planning may offer better accuracy and reliability, particularly for short stem prostheses. Improved surgical outcomes can be achieved with more precise preoperative planning using three-dimensional techniques.
JOURNAL OF ARTHROPLASTY
(2021)
Article
Oncology
Cristina L. Cotarelo, Annemarie Zschoeck-Manus, Marcus Schmidt, Arno Schad, Christoph Brochhausen, Charles James Kirkpatrick, Sonja Thaler
Summary: The intraoperative method combining touch imprint cytology (TIC) and subsequent frozen section (FS) analysis is highly reliable for detecting SLN macrometastases, allowing accurate identification of patients at high risk for extended axillary involvement during surgery and for accurate histological diagnosis of negative SLN.
CLINICAL & EXPERIMENTAL METASTASIS
(2021)
Article
Engineering, Biomedical
Sarah Al-Maawi, Mike Barbeck, Carlos Herrera Vizcaino, Rainer Egli, Robert Sader, Charles James Kirkpatrick, Marc Bohner, Shahram Ghanaati
Summary: The biological response of calcium phosphates is influenced by the presence of an alkaline layer on their surface during thermal treatment. Thermally treated alpha-TCP and beta-TCP granules stimulate fewer multinucleated giant cells and are more resistant to degradation compared to non-thermally treated granules. Heat treatment of alpha-TCP and beta-TCP granules shifts the reaction from foreign body response to physiological response, by reducing the number of induced multinucleated giant cells and enhancing degradation resistance.
ACTA BIOMATERIALIA
(2021)
Article
Biochemistry & Molecular Biology
Kathrin Maly, Enrique Andres Sastre, Eric Farrell, Andrea Meurer, Frank Zaucke
Summary: The study found that COMP and TSP-4 have different effects on chondrocyte migration, but both proteins promote the synthesis and matrix formation of cartilage, ultimately stabilizing the chondrocyte phenotype.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Orthopedics
Sebastian Braun, Jacques Mueller-Broich, Panagiotis Diaremes, Christoph Fleege, Andrea Meurer
Summary: The treatment of early onset scoliosis in children is complex, with conservative measures recommended for mild cases and surgical intervention considered for more severe cases. Surgical options like fusion may impede growth, while nonfusion techniques such as growing rods or VEPTR devices are alternatives. The best treatment approach depends on individual patient risk profiles and surgeon experience.
Article
Medicine, General & Internal
Stefan van Drongelen, Bernd J. Stetter, Harald Boehm, Felix Stief, Thorsten Stein, Andrea Meurer
Summary: Despite good clinical outcomes, deficits in gait biomechanics persist 2 years after total hip replacement surgery for patients with unilateral hip osteoarthritis. The study aimed to group patients showing similar gait adaptations and investigate the effect of surgery on gait kinematics and external joint forces. Results showed that patients with poorer preoperative gait patterns had worse gait kinematics after surgery, indicating the need for further research on tailored rehabilitation programs.
JOURNAL OF CLINICAL MEDICINE
(2021)
Review
Cell Biology
Rebecca Sohn, Gundula Roesch, Marius Junker, Andrea Meurer, Frank Zaucke, Zsuzsa Jenei-Lanzl
Summary: Adrenoceptors play a central role in mediating the effects of sympathetic neurotransmitters in the human body, especially in cardiovascular medicine. The sympathetic neurotransmitter NE contributes to the pathogenesis of osteoarthritis, with various AR subtypes expressed in joint tissues.
CELLULAR SIGNALLING
(2021)
Article
Neurosciences
Felix Stief, Jana Holder, Zoe Feja, Arian Lotfolahpour, Andrea Meurer, Jan Wilke
Summary: Increasing step width is an effective and simple gait mechanism to reduce frontal plane knee and hip joint moments, offering a potential noninvasive treatment option for patients with hip osteoarthritis. However, decreasing step width did not lead to significant changes in knee or hip joint loading, suggesting that hypothesis 2 was not supported in this study.
Article
Orthopedics
Markus Ehnert, Felix Stief, Andrea Meurer
Summary: Leg axes undergo physiological changes from birth to growth, with deformities considered as a pre-arthrosis. A detailed clinical examination is crucial to distinguish asymptomatic deformities from physiological axis deviations. Various conservative and surgical therapies are available, with interventions aimed at restoring anatomical alignment to prevent osteoarthritis. Surgical interventions may be necessary, including guided growth or osteotomies, to avoid early endoprosthetic replacement in adulthood.
Article
Chemistry, Physical
Sarah Al-Maawi, Xuejiu Wang, Robert Sader, Werner Goetz, Antonella Motta, Claudio Migliaresi, Charles James Kirkpatrick, Shahram Ghanaati
Summary: This study analyzed the long-term polarization pattern of macrophages and multinucleated giant cells (MNGCs) in the implantation areas of biomaterials, and found sustained expression of pro-inflammatory markers in SF-induced MNGCs over 180 days. This highlights the importance of tailored physicochemical properties to avoid extensive inflammatory reactions for clinical success.
Article
Engineering, Biomedical
Sarah Al-Maawi, Sandra Rother, Norbert Halfter, Karen M. Fiebig, Juliane Moritz, Stephanie Moeller, Matthias Schnabelrauch, Charles James Kirkpatrick, Robert Sader, Hans-Peter Wiesmann, Dieter Scharnweber, Vera Hintze, Shahram Ghanaati
Summary: This study aimed to functionalize a collagen-based scaffold with high-sulfated hyaluronan and investigate its properties in vitro and in vivo. The results showed that covalent binding of high-sulfated hyaluronan to collagen released only a marginal amount of the hyaluronan, while electrostatic binding released more. The covalent binding scaffolds showed reduced release of vascular endothelial growth factor and transforming growth factor beta 1, but increased release of interleukin-8 and epithelial growth factor compared to other scaffolds. In vivo, the covalent binding scaffolds reduced the proinflammatory macrophage response and did not induce multinucleated giant cell formation, while maintaining a stable structure and integration into the implantation region. This functionalized collagen scaffold has potential as a stable biomaterial for full-thickness skin regeneration.
BIOACTIVE MATERIALS
(2022)
