Article
Biochemistry & Molecular Biology
Yoshihiro Ishikawa, Arkadiusz Bonna, Douglas B. Gould, Richard W. Farndale
Summary: Mutations in the FKBP14 gene result in kyphoscoliotic Ehlers-Danlos Syndrome (EDS), and FKBP22 selectively binds to collagens III, IV, VI, and X. The binding distribution of FKBP22 along the collagen helix and its correlation with positive peptide charge have been observed.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Hitomi Nakao, Akira Seko, Yukishige Ito, Masafumi Sakono
Summary: This study found that the endoplasmic reticulum protein ERp29 may act as a bridge linking the lectin chaperones calnexin and calreticulin, forming complexes such as CNX-CRT. This not only facilitates the binding of CNX and CRT, but also reveals a new function of ERp29.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Review
Cell Biology
John C. Christianson, Ernst Jarosch, Thomas Sommer
Summary: Maintaining the integrity of the proteome is crucial for the survival of organisms. The secretory pathway of eukaryotes poses challenges for quality control, but the evolution has developed an elegant solution called ERAD through the ER-membrane-bound ubiquitylation machinery. ERAD is a specialized arm of protein quality control that ensures the efficient removal of aberrant or surplus proteins from the ER.
NATURE REVIEWS MOLECULAR CELL BIOLOGY
(2023)
Article
Multidisciplinary Sciences
Ranen Aviner, Kathy H. Li, Judith Frydman, Raul Andino
Summary: This study examines how viral pathogens manipulate host polysomes to synthesize viral proteins and hinder host protein production. By analyzing the interactions between viruses and host cells, the research identifies potential targets for selective antiviral interventions through targeting specialized viral polysomes.
Article
Cell & Tissue Engineering
Xiaoyu Yang, Wei Sun, Xiaoyan Jing, Qian Zhang, Hui Huang, Zuojun Xu
Summary: This study found that endoplasmic reticulum (ER) stress is associated with the behavior of lung resident mesenchymal/stromal cells (LR-MSC) during pulmonary fibrosis, and the ER stress responder C/EBP homologous protein (CHOP) plays a key role in this process. Targeting CHOP or using therapies based on CHOP knockdown LR-MSC may be promising ways to treat pulmonary fibrosis.
STEM CELL RESEARCH & THERAPY
(2022)
Review
Microbiology
Magali Roques, Annina Bindschedler, Raphael Beyeler, Volker T. Heussler
Summary: Plasmodium parasites have a complex life cycle alternating between a mosquito and a vertebrate host. They replicate in the liver and then infect red blood cells to produce more parasites. The review focuses on the cell division of Plasmodium parasite liver stage compared to other stages of the life cycle, especially the blood stage.
Article
Plant Sciences
Yuhong Zhou, Huiyang Yu, Yaping Tang, Rong Chen, Jinying Luo, Chunmei Shi, Shan Tang, Xin Li, Xinyan Shen, Rongfeng Chen, Yuyang Zhang, Yongen Lu, Zhibiao Ye, Liang Guo, Bo Ouyang
Summary: Plant mitochondrial fatty acid synthesis (mtFAS) plays an important role in tomato development. A mutant tomato was identified that displayed pleiotropic phenotypes and affected tomato morphogenesis by influencing photorespiration, energy supply, and reactive oxygen species homeostasis. The mtFAS pathway may modulate tomato development by influencing mitochondrial membrane lipid composition and other lipid metabolic pathways.
Article
Cell Biology
Vittorio Abbonante, Alessandro Malara, Martina Chrisam, Samuele Metti, Paolo Soprano, Claudio Semplicini, Luca Bello, Valeria Bozzi, Monica Battiston, Alessandro Pecci, Elena Pegoraro, Luigi De Marco, Paola Braghetta, Paolo Bonaldo, Alessandra Balduini
Summary: Endoplasmic reticulum stress is linked to collagen retention, apoptosis, and impaired autophagy in megakaryocytes. This study highlights the interplay between genetic disorders of collagens, endoplasmic reticulum stress, and autophagy regulation in megakaryocytes.
Review
Biochemistry & Molecular Biology
Sanaz Dastghaib, P. Sravan Kumar, Sajjad Aftabi, Gautam Damera, Azadeh Dalvand, Adel Sepanjnia, Mohammad Kiumarsi, Mohamad-Reza Aghanoori, Sukhwinder Singh Sohal, Sudharsana R. Ande, Javad Alizadeh, Pooneh Mokarram, Saeid Ghavami, Pawan Sharma, Amir A. Zeki
Summary: Lung cells are exposed to internal and external stressors, leading to disruption of protein homeostasis and activation of the UPR. Dysregulation of the UPR is associated with disease development and various human conditions. Compounds targeting the UPR pathway show potential for future therapeutic interventions.
AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Akshay Silswal, Apurba Lal Koner
Summary: We developed a far-red emitting fluorescence probe that can target the endoplasmic reticulum and sense its viscosity, which enables tracking of ER-phagy. Additionally, we investigated the alteration of ER viscosity during the ferroptosis process using intensity and lifetime-based spectroscopy and microscopy.
CHEMICAL COMMUNICATIONS
(2023)
Article
Cell Biology
William Kasberg, Peter Luong, Michael G. Hanna, Kayla Minushkin, Annabelle Tsao, Raakhee Shankar, Samuel Block, Anjon Audhya
Summary: Coat protein complex II (COPII) is crucial for the packaging of secretory cargoes in membrane-enclosed transport carriers. This study demonstrates that anterograde cargo transport from the endoplasmic reticulum (ER) can still occur even when the Sar1 protein is depleted, although with reduced efficiency. The findings highlight alternative mechanisms by which COPII promotes transport carrier biogenesis.
Article
Plant Sciences
Andras Sandor, Marketa Samalova, Federica Brandizzi, Verena Kriechbaumer, Ian Moore, Mark D. Fricker, Lee J. Sweetlove
Summary: In this study, we characterized membranous compartments derived from the endoplasmic reticulum (ER) in tobacco. We found that the morphology of the final compartment is affected by the introduction of polyproteins into the ER membrane, and a cytosol-facing oligomerization domain is essential for compartment formation. We also discovered the existence of a diffusional barrier between the compartment, ER, and cytosol, and showed that the presence of the compartment does not disrupt the rest of the ER network.
JOURNAL OF EXPERIMENTAL BOTANY
(2023)
Article
Multidisciplinary Sciences
Timothy J. Hawkins, Michaela Kopischke, Patrick J. Duckney, Katarzyna Rybak, David A. Mentlak, Johan T. M. Kroon, Mai Thu Bui, A. Christine Richardson, Mary Casey, Agnieszka Alexander, Geert De Jaeger, Monika Kalde, Ian Moore, Yasin Dagdas, Patrick J. Hussey, Silke Robatzek
Summary: This study reveals that NET4A and NET4B members of the NET family play an essential role in the reorganization of guard cell actin during stomatal closure, mediating the molecular link between actin filaments and the tonoplast through interaction with RABG3b.
NATURE COMMUNICATIONS
(2023)
Article
Plant Sciences
Maria De Benedictis, Antonia Gallo, Danilo Migoni, Paride Papadia, Pietro Roversi, Angelo Santino
Summary: We studied the response of Arabidopsis thaliana to chronic and temporary Cd2+ stress, finding that Cd2+ induces ER stress and unfolded protein response (UPR) through the bZIP60 arm. The UPR activation is concentration- or time-dependent and leads to the activation of ER stress marker genes. Severe Cd2+ stress triggers programmed cell death (PCD) through NAC089 activation. The Atbzip28/bzip60 double mutant shows reduced UPR and PCD, as well as enhanced Cd2+ tolerance, possibly due to increased brassinosteroids signaling.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Nadia Garibaldi, Barbara M. Contento, Gabriele Babini, Jacopo Morini, Stella Siciliani, Marco Biggiogera, Mario Raspanti, Joan C. Marini, Antonio Rossi, Antonella Forlino, Roberta Besio
Summary: The chemical chaperone 4-phenylbutyrate (4-PBA) has been shown to improve osteoblast homeostasis and bone matrix quality in osteogenesis imperfecta (OI), indicating its potential as a treatment for OI.
Article
Multidisciplinary Sciences
S. Pillay, N. L. Meyer, A. S. Puschnik, O. Davulcu, J. Diep, Y. Ishikawa, L. T. Jae, J. E. Wosen, C. M. Nagamine, M. S. Chapman, J. E. Carette
Article
Multidisciplinary Sciences
Yoshihiro Ishikawa, Shinya Ito, Kazuhiro Nagata, Lynn Y. Sakai, Hans Peter Bachinger
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2016)
Article
Biochemistry & Molecular Biology
Yoshihiro Ishikawa, Paul Holden, Hans Peter Bachinger
JOURNAL OF BIOLOGICAL CHEMISTRY
(2017)
Article
Biochemistry & Molecular Biology
Yoshihiro Ishikawa, Kazunori Mizuno, Hans Peter Bachinger
JOURNAL OF BIOLOGICAL CHEMISTRY
(2017)
Article
Biochemistry & Molecular Biology
Yoshihiro Ishikawa, Kristofer Rubin, Hans Peter Baechinger, Sebastian Kalamajski
JOURNAL OF BIOLOGICAL CHEMISTRY
(2018)
Article
Biochemistry & Molecular Biology
Sanne D'hondt, Brecht Guillemyn, Delfien Syx, Sofie Symoens, Riet De Rycke, Leen Vanhoutte, Wendy Toussaint, Bart N. Lambrecht, Anne De Paepe, Douglas R. Keene, Yoshihiro Ishikawa, Hans Peter Bachinger, Sophie Janssens, Mathieu J. M. Bertrand, Fransiska Malfait
Article
Biochemistry & Molecular Biology
Narendra Bharathy, Noah E. Berlow, Eric Wang, Jinu Abraham, Teagan P. Settelmeyer, Jody E. Hooper, Matthew N. Svalina, Yoshihiro Ishikawa, Keith Zientek, Zia Bajwa, Martin W. Goros, Brian S. Hernandez, Johannes E. Wolff, Michelle A. Rudek, Linping Xu, Nicole M. Anders, Ranadip Pal, Alexandria P. Harrold, Angela M. Davies, Arya Ashok, Darnell Bushby, Maria Mancini, Christopher Noakes, Neal C. Goodwin, Peter Ordentlich, James Keck, Douglas S. Hawkins, Erin R. Rudzinski, Bishwanath Chatterjee, Hans Peter Bachinger, Frederic G. Barr, Jennifer Liddle, Benjamin A. Garcia, Atiya Mansoor, Theodore J. Perkins, Christopher R. Vakoc, Joel E. Michalek, Charles Keller
Article
Multidisciplinary Sciences
Yoshihiro Ishikawa, Nobuyo Mizuno, Paul Holden, Pei Jin Lim, Douglas B. Gould, Marianne Rohrbach, Cecilia Giunta, Hans Peter Bachinger
SCIENTIFIC REPORTS
(2020)
Article
Biochemistry & Molecular Biology
Yoshihiro Ishikawa, Yuki Taga, Keith Zientek, Nobuyo Mizuno, Antti M. Salo, Olesya Semenova, Sara F. Tufa, Douglas R. Keene, Paul Holden, Kazunori Mizuno, Douglas B. Gould, Johanna Myllyharju, Hans Peter Bachinger
Summary: Collagen is the most abundant protein in humans, with a complex biosynthesis involving multiple enzymes and chaperones. LH1 and P3H3 play distinct but related roles in lysyl hydroxylation of collagen, with LH1 having a global enzymatic role and P3H3 being involved particularly at cross-link formation sites.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Article
Genetics & Heredity
Delfien Syx, Yoshihiro Ishikawa, Jan Gebauer, Sergei P. Boudko, Brecht Guillemyn, Tim Van Damme, Sanne D'hondt, Sofie Symoens, Sheela Nampoothiri, Douglas B. Gould, Ulrich Baumann, Hans Peter Bachinger, Fransiska Malfait
Summary: Osteogenesis imperfecta is caused by a homozygous p.(R222S) substitution in the HSP47 gene, resulting in significantly reduced affinity of HSP47-R222S for type I collagen and leading to overmodification of type I collagen. Unlike other types of OI, this overmodification is not due to prolonged exposure of collagen to modifying enzymes, as the intracellular folding rate of type I collagen appears to be normal. Abnormal binding of HSP47-R222S results in upregulation and increased binding of molecular chaperones and collagen-modifying enzymes, suggesting a compensatory mechanism for aberrant binding and eventually leading to overmodification of type I collagen chains.
Article
Biophysics
Alaa Al-Shaer, Aaron Lyons, Yoshihiro Ishikawa, Billy G. Hudson, Sergei P. Boudko, Nancy R. Forde
Summary: The mechanical properties of collagen IV, an integral component of basement membranes, have been less studied compared to fibril-forming collagens. The presence of interruptions in the triple-helix-defining sequence along its collagenous domain leads to highly heterogeneous mechanics in collagen IV, while sequence variations within the triple helix contribute to variable flexibility in collagen III. Physiological changes in pH and chloride concentration do not alter the flexibility of collagen IV, indicating that environmental factors may not control its compaction during secretion.
BIOPHYSICAL JOURNAL
(2021)
Article
Cell Biology
Stefan Preisendoerfer, Yoshihiro Ishikawa, Elisabeth Hennen, Stephan Winklmeier, Jonas C. Schupp, Larissa Knueppel, Isis E. Fernandez, Leonhard Binzenhofer, Andrew Flatley, Brenda M. Juan-Guardela, Clemens Ruppert, Andreas Guenther, Marion Frankenberger, Rudolf A. Hatz, Nikolaus Kneidinger, Jurgen Behr, Regina Feederle, Aloys Schepers, Anne Hilgendorff, Naftali Kaminski, Edgar Meinl, Hans Peter Baechinger, Oliver Eickelberg, Claudia A. Staab-Weijnitz
Summary: The study reveals that FKBP11 plays an important role in plasma cells, is regulated by XBP1, and sheds light on the important mechanisms involved in antibody production and folding.
Article
Biochemistry & Molecular Biology
Yoshihiro Ishikawa, Yuki Taga, Thibault Coste, Sara F. Tufa, Douglas R. Keene, Kazunori Mizuno, Elisabeth Tournier-Lasserve, Douglas B. Gould
Summary: Collagens are important proteins in the body, and mutations in genes encoding them can cause connective tissue disorders. Lysyl hydroxylase 3 (LH3) plays a critical role in collagen biosynthesis, and LH3 pathogenic variants may contribute to Gould syndrome.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Yoshihiro Ishikawa, Arkadiusz Bonna, Douglas B. Gould, Richard W. Farndale
Summary: Mutations in the FKBP14 gene result in kyphoscoliotic Ehlers-Danlos Syndrome (EDS), and FKBP22 selectively binds to collagens III, IV, VI, and X. The binding distribution of FKBP22 along the collagen helix and its correlation with positive peptide charge have been observed.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Larissa Knueppel, Yoshihiro Ishikawa, Michaela Aichler, Katharina Heinzelmann, Rudolf Hatz, Juergen Behr, Axel Walch, Hans Peter Baechinger, Oliver Eickelberg, Claudia A. Staab-Weijnitz
AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY
(2017)