4.6 Article

Fe-S Cluster Biogenesis in Isolated Mammalian Mitochondria COORDINATED USE OF PERSULFIDE SULFUR AND IRON AND REQUIREMENTS FOR GTP, NADH, AND ATP

期刊

JOURNAL OF BIOLOGICAL CHEMISTRY
卷 290, 期 1, 页码 640-657

出版社

AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
DOI: 10.1074/jbc.M114.610402

关键词

Friedreich Ataxia; Iron; Iron Metabolism; Iron-Sulfur Protein; Metal Homeostasis; Mitochondria; Mitochondrial Aconitase; Mitochondrial Metabolism; Sulfur; Yeast

资金

  1. National Institutes of Health [R01GM107542, R37DK053953]
  2. Friedreich's Ataxia Research Alliance grant

向作者/读者索取更多资源

Background: Fe-S cluster assembly in mitochondria involves generation of an activated form of sulfur called persulfide. Results: A novel experimental system tracks new Fe-S cluster synthesis in isolated mammalian mitochondria. Conclusion: The use of persulfide sulfur and iron for Fe-S cluster biogenesis is tightly coordinated by processes requiring GTP, NADH, and ATP. Significance: These cofactor targets can now be defined. Iron-sulfur (Fe-S) clusters are essential cofactors, and mitochondria contain several Fe-S proteins, including the [4Fe-4S] protein aconitase and the [2Fe-2S] protein ferredoxin. Fe-S cluster assembly of these proteins occurs within mitochondria. Although considerable data exist for yeast mitochondria, this biosynthetic process has never been directly demonstrated in mammalian mitochondria. Using [S-35]cysteine as the source of sulfur, here we show that mitochondria isolated from Cath.A-derived cells, a murine neuronal cell line, can synthesize and insert new Fe-S-35 clusters into aconitase and ferredoxins. The process requires GTP, NADH, ATP, and iron, and hydrolysis of both GTP and ATP is necessary. Importantly, we have identified the S-35-labeled persulfide on the NFS1 cysteine desulfurase as a genuine intermediate en route to Fe-S cluster synthesis. In physiological settings, the persulfide sulfur is released from NFS1 and transferred to a scaffold protein, where it combines with iron to form an Fe-S cluster intermediate. We found that the release of persulfide sulfur from NFS1 requires iron, showing that the use of iron and sulfur for the synthesis of Fe-S cluster intermediates is a highly coordinated process. The release of persulfide sulfur also requires GTP and NADH, probably mediated by a GTPase and a reductase, respectively. ATP, a cofactor for a multifunctional Hsp70 chaperone, is not required at this step. The experimental system described here may help to define the biochemical basis of diseases that are associated with impaired Fe-S cluster biogenesis in mitochondria, such as Friedreich ataxia.

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