Article
Biochemistry & Molecular Biology
Beyenech Binotti, Reinhard Jahn, Angel Perez-Lara
Summary: Chemical neurotransmission is the main way neurons communicate, with neurotransmitters released from synaptic vesicles via exocytosis into the synaptic cleft. Protein machineries are responsible for neurotransmitter release and recycling, while the role of lipids in synaptic transmission and the composition of synaptic vesicle membrane lipids have received less attention.
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
(2021)
Article
Biochemistry & Molecular Biology
Christiana Kontaxi, Nawon Kim, Michael A. Cousin
Summary: The protein Amph1 plays an important role in coordinating endocytosis in non-neuronal cells and synaptic vesicles at central nerve terminals. It interacts with lipids and proteins, with all interactions except for the PRD domain required for synaptic vesicle endocytosis. This study reveals that the interaction between the PRD domain of Amph1 and endophilin A1 is essential for efficient synaptic vesicle endocytosis.
JOURNAL OF NEUROCHEMISTRY
(2023)
Article
Multidisciplinary Sciences
Sue Han, Xin Wang, Nicholas Cordero, Ling-Gang Wu
Summary: This study demonstrates the detection of three fusion modes in cells using a combination of confocal microscopy and patch-clamp recording. The fusion modes include close-fusion, stay-fusion, and shrink-fusion, and they were detected by labeling the plasma membrane, loading vesicles with a fluorescent probe, and analyzing the fluorescence measurements.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2022)
Article
Cell Biology
Zhang Lin, Yu Wang, Dong Yongming, Aaradhya Pant, Liu Yan, Laura Masserman, Xu Ye, Richard N. McLaughlin, Bai Jihong
Summary: The functional specificity of curvature-sensing amphipathic motifs in Caenorhabditis elegans was investigated by studying endophilin, an endocytic protein for synaptic vesicle recycling. The results showed that the role of amphipathic motifs cannot simply be extrapolated from the identity of their parental proteins. Non-functional endophilin chimeras exhibited similar defects, producing fewer synaptic vesicles but more endosomes. Reprogramming the functional specificity of curvature-sensing motifs in vivo was successfully achieved by changing the cationic property of amphipathic motifs.
DEVELOPMENTAL CELL
(2022)
Article
Neurosciences
Wonchul Shin, Lisi Wei, Gianvito Arpino, Lihao Ge, Xiaoli Guo, Chung Yu Chan, Edaeni Hamid, Oleg Shupliakov, Christopher K. E. Bleck, Ling-Gang Wu
Summary: This study reveals that endocytosis is mainly mediated by calcium-triggered and dynamin-mediated closure of Omega profiles and fusion pores, leading to the formation of Lambda-shaped, Omega-shaped, and O-shaped vesicles, rather than the traditional flat-to-round membrane transformation.
Review
Biochemistry & Molecular Biology
Melina Overhoff, Elodie De Bruyckere, Natalia L. Kononenko
Summary: Neurons rely on the autophagy and endocytosis pathways for cellular homeostasis and function, with defects in these pathways linked to the pathology of neurodegenerative diseases.
JOURNAL OF NEUROCHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Robert V. Chimenti, James Daley, James Sack, Jennifer Necsutu, Nicholas Whiting
Summary: The application of parahydrogen gas in enhancing magnetic resonance signals of diverse chemical species has significantly increased in the past decade. Parahydrogen can be enriched beyond its normal abundance by lowering the temperature of hydrogen gas in the presence of a catalyst. However, its reconversion rate to the normal isomeric ratio is accelerated in glass containers due to paramagnetic impurities. The study investigates the effect of surfactant coatings on the reconversion rate of parahydrogen in valved borosilicate glass NMR sample tubes, and most surfactants increase the reconversion time compared to untreated sample tubes.
Article
Neurosciences
Archan Ganguly, Rohan Sharma, Nicholas P. Boyer, Florian Wernert, Sebastien Phan, Daniela Boassa, Leonardo Parra, Utpal Das, Ghislaine Caillol, Xuemei Han, John R. Yates, Mark H. Ellisman, Christophe Leterrier, Subhojit Roy
Summary: Clathrin in neurons forms stable transport packets in axons, moving intermittently on microtubules, and at synapses, multiple clathrin packets abut synaptic vesicle clusters and exchange between synaptic boutons in a microtubule-dependent superpool.
Article
Biochemistry & Molecular Biology
Alexander Johnson, Walter A. Kaufmann, Christoph Sommer, Tommaso Costanzo, Dana A. Dahhan, Sebastian Y. Bednarek, Jiri Friml
Summary: Biological systems are made up of dynamic three-dimensional parts. This study introduces novel methods to analyze biological structures and focuses on Arabidopsis clathrin-coated vesicles (CCVs). The methods allow for visualization of CCVs in unroofed cells and biochemical isolated organelles at high resolution, and also provide a deep learning analysis method for screening CCVs in metal replicated samples.
Article
Cell Biology
Hyo Young Jung, Hyun Jung Kwon, Woosuk Kim, In Koo Hwang, Goang-Min Choi, In Bok Chang, Dae Won Kim, Seung Myung Moon
Summary: Tat-SH3GL2 protects neurons from oxidative and ischemic damage by reducing lipid peroxidation and inflammation, and improving synaptic plasticity after ischemia.
Article
Multidisciplinary Sciences
Wonchul Shin, Ben Zucker, Nidhi Kundu, Sung Hoon Lee, Bo Shi, Chung Yu Chan, Xiaoli Guo, Jonathan T. Harrison, Jaymie Moore Turechek, Jenny E. Hinshaw, Michael M. Kozlov, Ling-Gang Wu
Summary: The process of membrane budding is essential for cell survival, but the forces involved in non-coated membrane budding have remained unclear. This study reveals how non-coated membrane budding is mediated, involving actin filaments and dynamin to generate the necessary forces. These mechanisms control the speed, size, and number of vesicles formed, and have implications for various membrane remodeling processes.
NATURE COMMUNICATIONS
(2022)
Article
Multidisciplinary Sciences
Carolina Gomis Perez, Natasha R. Dudzinski, Mason Rouches, Ane Landajuela, Benjamin Machta, David Zenisek, Erdem Karatekin
Summary: Membrane tension gradients play a crucial role in cellular activities such as cell migration, division, phagocytosis, and exo-endocytosis. The speed at which membrane tension equilibrates can vary significantly depending on the cell type. Rapid synaptic vesicle turnover in neuronal terminals leads to quick membrane tension equilibration, while neuroendocrine adrenal chromaffin cells have slower recovery rates.
Article
Neurosciences
Soulmee Koh, Wongyoung Lee, Sang Myun Park, Sung Hyun Kim
Summary: The study demonstrates the important role of Cav1 in synaptic vesicle exocytosis, with its knockdown significantly impairing synaptic vesicle function. Neurons rescued by triple mutants lacking palmitoylation sites of Cav1 show impairments in both synaptic transmission and retrieval, highlighting the importance of Cav1 palmitoylation in activity-driven synaptic vesicle dynamics.
Article
Chemistry, Physical
Arijit Mahapatra, Padmini Rangamani
Summary: Plasma membrane tubes are common in cellular membranes and organelle membranes, and play important roles in trafficking, ion transport, and cellular motility. They can be formed by localized forces or induced by membrane-bound proteins. A mathematical framework to model cylindrical tubular protrusions formed by proteins with anisotropic spontaneous curvature is presented. The analysis reveals that the tube radius depends on effective tension including membrane tension and protein-induced curvature. The findings have implications for processes like endocytosis, t-tubule formation in myocytes, and cristae formation in mitochondria.
Article
Chemistry, Physical
Benedetta Come, Maressa Donato, Lucia Francesca Potenza, Paolo Mariani, Rosangela Itri, Francesco Spinozzi
Summary: Rhamnolipids, produced by Pseudomonas aeruginosa, are glycolipids that act as biosurfactants. Their mechanism of action in biological systems is not well defined, but experimental results suggest that they may alter membrane curvature and structure, potentially affecting endocytic processes in cells.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2021)
Article
Cell Biology
Zhiqi Sun, Hui-Yuan Tseng, Steven Tan, Fabrice Senger, Laetitia Kurzawa, Dirk Dedden, Naoko Mizuno, Anita A. Wasik, Manuel Thery, Alexander R. Dunn, Reinhard Faessler
NATURE CELL BIOLOGY
(2016)
Meeting Abstract
Biophysics
Naoko Mizuno
BIOPHYSICAL JOURNAL
(2018)
Article
Cell Biology
Nirakar Basnet, Hana Nedozralova, Alvaro H. Crevenna, Satish Bodakuntla, Thomas Schlichthaerle, Michael Taschner, Giovanni Cardone, Carsten Janke, Ralf Jungmann, Maria M. Magiera, Christian Biertuempfel, Naoko Mizuno
NATURE CELL BIOLOGY
(2018)
Article
Multidisciplinary Sciences
Qianmin Wang, Michael Taschner, Kristina A. Ganzinger, Charlotte Kelley, Alethia Villasenor, Michael Heymann, Petra Schwille, Esben Lorentzen, Naoko Mizuno
NATURE COMMUNICATIONS
(2018)
Article
Biochemistry & Molecular Biology
Dirk Dedden, Stephanie Schumacher, Charlotte F. Kelley, Martin Zacharias, Christian Biertuempfel, Reinhard Faessler, Naoko Mizuno
Article
Cell Biology
Thomas G. Biel, Baikuntha Aryal, Michael H. Gerber, Jose G. Trevino, Naoko Mizuno, V. Ashutosh Rao
CELL DEATH & DISEASE
(2020)
Article
Biology
Charlotte F. Kelley, Thomas Litschel, Stephanie Schumacher, Dirk Dedden, Petra Schwille, Naoko Mizuno
Review
Biochemistry & Molecular Biology
Stephanie Schumacher, Roberto Vazquez Nunez, Christian Biertumpfel, Naoko Mizuno
Summary: Focal adhesions are large macromolecular assemblies essential for cellular events like migration, polarization, and cancer formation. Hundreds of cellular players gather at FA sites in response to external stimuli, forming a complex network during cell migration. The intricate FA network formation complicates the understanding of individual molecular actions in cellular experiments.
Article
Multidisciplinary Sciences
Thomas Litschel, Charlotte F. Kelley, Danielle Holz, Maral Adeli Koudehi, Sven Kenjiro Vogel, Laura Burbaum, Naoko Mizuno, Dimitrios Vavylonis, Petra Schwille
Summary: Researchers have successfully studied bundled actin filaments in Giant Unilamellar Vesicles using advanced encapsulation methods and compared the results with theoretical modeling. By changing key parameters, they were able to simulate actin polymerization to resemble various types of networks in living cells.
NATURE COMMUNICATIONS
(2021)
Article
Multidisciplinary Sciences
Stephanie Schumacher, Dirk Dedden, Roberto Vazquez Nunez, Kyoko Matoba, Junichi Takagi, Christian Biertuempfel, Naoko Mizuno
Summary: The interaction mechanism between Integrin alpha(5)beta(1) and fibronectin was studied, revealing significant conformational changes upon complex formation. Additionally, the resting state of the integrin adopts an incompletely bent conformation, challenging the existing model.
Review
Cell Biology
Satish Bodakuntla, Hana Nedozralova, Nirakar Basnet, Naoko Mizuno
Summary: Axon branching is a critical process for neural network formation, requiring a local system to dynamically control and regulate axonal growth. Regulation of axon branching involves coordination of cellular functions such as cytoskeleton and membrane remodeling.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Correction
Multidisciplinary Sciences
Thomas Litschel, Charlotte F. Kelley, Danielle Holz, Maral Adeli Koudehi, Sven K. Vogel, Laura Burbaum, Naoko Mizuno, Dimitrios Vavylonis, Petra Schwille
NATURE COMMUNICATIONS
(2022)
Editorial Material
Neurosciences
Trisha Gura, Amparo Acker-Palmer, Alex Kolodkin, Rob Meijers, Naoko Mizuno, Elena Seiradake, Marc Tessier-Lavigne
Summary: The Molecular Neurobiology Workshop in Crete brought together an interdisciplinary group of scientists to share data and explore the assembly of the nervous system in response to stimuli.
Article
Multidisciplinary Sciences
Christopher Cyrus Kuhn, Nirakar Basnet, Satish Bodakuntla, Pelayo Alvarez-Brecht, Scott Nichols, Antonio Martinez-Sanchez, Lorenzo Agostini, Young-Min Soh, Junichi Takagi, Christian Biertuempfel, Naoko Mizuno
Summary: SARS-CoV-2 spike protein (S protein) interacts with host-cell receptors and activates platelets, potentially contributing to the pathogenesis and coagulopathies in COVID-19. The study demonstrates the direct interaction between S protein and platelets, leading to their irreversible activation and the formation of filopodia. The weak interactions between S protein and integrin receptor in platelets are hypothesized to be the cause of stochastic platelet activation and severe coagulopathies.
NATURE COMMUNICATIONS
(2023)
Meeting Abstract
Biophysics
Thomas Litschel, Charlotte F. Kelley, Danielle Holz, Naoko Mizuno, Dimitrios Vavylonis, Petra Schwille
EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS
(2021)
