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Biochemistry & Molecular Biology
Adele Mossa, Jessica Pagano, Luisa Ponzoni, Alessandro Tozzi, Elena Vezzoli, Miriam Sciaccaluga, Cinzia Costa, Stefania Beretta, Maura Francolini, Mariaelvina Sala, Paolo Calabresi, Tobias M. Boeckers, Carlo Sala, Chiara Verpelli
Summary: Mutations in SHANK family genes in humans are associated with autism spectrum disorders and intellectual disability. A new mouse model with simultaneous deletion of Shank1 and Shank3 showed low survival rate, reduced activation of signaling pathways, and severe behavioral impairments, suggesting the importance of Shank proteins in postnatal brain development and synaptic plasticity.
MOLECULAR PSYCHIATRY
(2021)
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Biology
Yuki Ono, Kouki Kawakami, Gaku Nakamura, Satoru Ishida, Junken Aoki, Asuka Inoue
Summary: A HEK293 cell line lacking all G alpha i subunits is generated, allowing for the measurement of coupling between a GPCR and individual G alpha i subunits upon transient re-expression of a specific G alpha i subunit. Coupling selectivity across 11 GPCRs is analyzed by measuring ligand-induced inhibitory activity for cAMP accumulation. Results indicate that GPCRs fine-tune G alpha i signaling by exerting coupling preference at the G alpha i-subunit level.
COMMUNICATIONS BIOLOGY
(2023)
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Neurosciences
Andres Benitez-Rosendo, Patricia Lagos, Karina Cal, Laura Colman, Carlos Escande, Aldo Calliari
Summary: DBC1 plays a crucial role in energy metabolism, cell cycle progression, and cancer cell regulation. This study reveals the impact of DBC1 on cell cycle and neurogenesis, suggesting its involvement in neuroblast generation and neural differentiation, as well as its association with cognitive functions such as learning and memory formation.
MOLECULAR AND CELLULAR NEUROSCIENCE
(2022)
Article
Genetics & Heredity
Tamara Jarayseh, Brecht Guillemyn, Hanna De Saffel, Jan Willem Bek, Delfien Syx, Sofie Symoens, Yannick Gansemans, Filip Nieuwerburgh, Sujatha Jagadeesh, Jayarekha Raja, Fransiska Malfait, Paul J. Coucke, Adelbert De Clercq, Andy Willaert
Summary: Bi-allelic mutations in TAPT1 gene lead to a wide range of phenotypes, from severe skeletal and congenital abnormalities to isolated early-onset cataract. A frameshift mutation in the TAPT1 gene was identified in a patient with bilateral early-onset cataract and skeletal abnormalities, expanding the phenotypic spectrum. A zebrafish model with TAPT1 gene knockout exhibited eye malformations, loss of vision, increased photokinetics, and hyperpigmentation, providing insights into the ultrastructural and molecular characteristics of TAPT1 mutations.
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Biochemistry & Molecular Biology
Tony Hunter
Summary: Protein phosphorylation is a reversible post-translational modification involving various natural amino acids. While serine, threonine, and tyrosine phosphorylation have been extensively studied, less is known about non-canonical phosphorylations such as histidine phosphorylation. Recent advances in research tools have accelerated our understanding of the multifaceted functions of histidine phosphorylation in cellular processes.
Article
Multidisciplinary Sciences
Paulina M. Getsy, Sripriya Sundararajan, Walter J. May, Graham C. von Schill, Dylan K. McLaughlin, Lisa A. Palmer, Stephen J. Lewis
Summary: The study found that the loss of the eNOS gene affects the ventilatory responses during and after a hypercapnic gas challenge in male C57BL6 mice, while female mice can compensate for the loss of eNOS with regards to triggering ventilatory responses to the challenge.
SCIENTIFIC REPORTS
(2021)
Article
Multidisciplinary Sciences
Boil Kim, Jihoon Kim, Minjeong Chun, Inah Park, Damhyeon Kwak, Mijung Choi, Kyungjin Kim, Han Kyoung Choe
Summary: The mammalian molecular clock relies on a complex transcription-translation feedback loop with genetic redundancy among essential clock genes for robustness, where the CSAC serves as a simple yet effective tool for exploring the molecular clock in vivo.
SCIENTIFIC REPORTS
(2021)
Article
Multidisciplinary Sciences
Paulina M. Getsy, Sripriya Sundararajan, Walter J. May, Graham C. von Schill, Dylan K. McLaughlin, Lisa A. Palmer, Stephen J. Lewis
Summary: The study demonstrates the significant role of eNOS in ventilatory responses to hypoxic gas challenge in female C57BL6 mice compared to males, and in the expression of post-HXC responses in male C57BL6 mice. These findings indicate that sex and specific proteins like eNOS have profound influences on ventilatory processes and responses to hypoxic gas challenges.
SCIENTIFIC REPORTS
(2021)
Article
Pharmacology & Pharmacy
Lan Ma, Xiaoce Dai, Chenxia Wu, Mingshuang Li, Hongzhuan Sheng, Wei Mao
Summary: The Tanyu Tongzhi Formula (TTF) was shown to reduce inflammatory factor levels, lipid content, and increase collagen/lipid ratio in atherosclerotic plaques by promoting alternative macrophage activation through PPAR gamma expression and inhibiting AKT/ERK phosphorylation, thus delaying atherosclerotic plaque progression.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Psychiatry
Sandra T. Suess, Linda M. Olbricht, Stefan Herlitze, Katharina Spoida
Summary: In this study, the researchers used a 5-HT2CR knock-out mouse model to investigate the role of 5-HT2CRs in the fear circuitry. The results showed that the loss of 5-HT2CRs exclusively accelerated fear extinction without affecting fear acquisition and fear expression. They also discovered alterations in neuronal activity in specific subregions of the DRN and the BNST in the knock-out mice. Additionally, they identified a specific serotonergic pathway from the DRC to the BNSTad that showed increased neuronal activity in the knock-out mice. These findings provide new insights into the fear extinction network and have important implications for the treatment of PTSD.
TRANSLATIONAL PSYCHIATRY
(2022)
Article
Biochemistry & Molecular Biology
Jhana O. Hendrickx, Sofie De Moudt, Elke Calus, Peter Paul De Deyn, Debby Van Dam, Guido R. Y. De Meyer
Summary: This study investigates the association between arterial stiffness and cognitive decline by introducing arterial stiffness in genetic and pharmacological NO dysfunction models. The findings demonstrate that non-selective inhibition of NOS activity leads to cardiac dysfunction, arterial stiffness, and decline in hippocampal-dependent learning and memory, highlighting the importance of neuronal NOS in both cardiovascular and neurological pathophysiology.
Article
Neurosciences
Inge C. M. Hoogland, Jutka Yik, Dunja Westhoff, Joo-Yeon Engelen-Lee, Merche Valls Seron, Wing-Kit Man, Judith H. P. M. Houben-Weerts, Michael W. Tanck, David J. van Westerloo, Tom van der Poll, Willem A. Gool, Diederik van de Beek
Summary: This study found that loss of function of TREM2 during systemic infection leads to an increased number of activated microglia in the thalamus, but a consistent increase in expression of inflammatory genes in the brain was not observed. The role of TREM2 in the neuro-inflammatory response following systemic infection appears to be limited.
NEUROSCIENCE LETTERS
(2022)
Article
Biochemical Research Methods
Isaac Fradkin, Eran Eldar
Summary: People can reject and replace unwanted thoughts after they have reached consciousness to prevent their continuous emergence. Additionally, individuals are capable of controlling the strengthening of associations during repetition. These individual differences may explain difficulties faced by some individuals with unwanted and intrusive thoughts.
PLOS COMPUTATIONAL BIOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Sherko Nasseri, Sara Parsa, Zakaria Vahabzadeh, Babak Baban, Mohammad Bagher Khademerfan, Bahram Nikkhoo, Mohammad Rastegar Khosravi, Saman Bahrami, Fardin Fathi
Summary: This study demonstrates that the deficiency of Fam83h disrupts dental mineralization and formation by concurrently reducing the expression levels of Wnt/β-catenin mediators and mineralization-related genes. These findings suggest a cumulative effect and multi-factorial interplay between Fam83h, Wnt/β-catenin signaling, and dental mineralization-related genes during tooth formation.
IRANIAN JOURNAL OF BIOTECHNOLOGY
(2023)
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Biochemistry & Molecular Biology
Saswati Panda, Shekhar Srivastava, Zhai Li, Martin Vaeth, Stephen R. Fuhs, Tony Hunter, Edward Y. Skolnik
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Biology
Shekhar Srivastava, Saswati Panda, Zhai Li, Stephen R. Fuhs, Tony Hunter, Dennis J. Thiele, Stevan R. Hubbard, Edward Y. Skolnik
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Multidisciplinary Sciences
Marianne Strazza, Inbar Azoulay-Alfaguter, Michael Peled, Alan V. Smrcka, Edward Y. Skolnik, Shekhar Srivastava, Adam Mor
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2017)
Article
Endocrinology & Metabolism
Shekhar Srivastava, Zhai Li, Irfana Soomro, Ying Sun, Jianhui Wang, Li Bao, William A. Coetzee, Charles A. Stanley, Chonghong Li, Edward Y. Skolnik
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Cell Biology
Ja Young Kim-Muller, Shangang Zhao, Shekhar Srivastava, Yves Mugabo, Hye-Lim Noh, YoungJung R. Kim, S. R. Murthy Madiraju, Anthony W. Ferrante, Edward Y. Skolnik, Marc Prentki, Domenico Accili
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Urology & Nephrology
Mamdouh Albaqumi, Shekhar Srivastava, Zhai Li, Olga Zhdnova, Heike Wulff, Omar Itani, Darren P. Wallace, Edward Y. Skolnik
KIDNEY INTERNATIONAL
(2008)
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Urology & Nephrology
Olga Zhdanova, Shekhar Srivastava, Lie Di, Zhai Li, Leila Tchelebi, Sara Dworkin, Duncan B. Johnstone, Jiri Zavadil, Mark M. Chong, Dan R. Littman, Lawrence B. Holzman, Laura Barisoni, Edward Y. Skolnik
KIDNEY INTERNATIONAL
(2011)
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Biochemistry & Molecular Biology
Shekhar Srivastava, Xinjiang Cai, Zhai Li, Yi Sun, Edward Y. Skolnik
MOLECULAR AND CELLULAR BIOLOGY
(2012)
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Cell Biology
Shekhar Srivastava, Lie Di, Olga Zhdanova, Zhai Li, Santosha Vardhana, Qi Wan, Ying Yan, Rajat Varma, Jonathan Backer, Heike Wulff, Michael L. Dustin, Edward Y. Skolnik
MOLECULAR BIOLOGY OF THE CELL
(2009)
Article
Cell Biology
Xinjiang Cai, Shekhar Srivastava, Sheena Surindran, Zhai Li, Edward Y. Skolnik
MOLECULAR BIOLOGY OF THE CELL
(2014)
Article
Multidisciplinary Sciences
Shekhar Srivastava, Zhai Li, Edward Y. Skolnik
Article
Multidisciplinary Sciences
Shekhar Srivastava, Olga Zhdanova, Lie Di, Zhai Li, Mamdouh Albaqumi, Heike Wulff, Edward Y. Skolnik
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2008)
Article
Multidisciplinary Sciences
Lie Di, Shekhar Srivastava, Olga Zhdanova, Yi Ding, Zhai Li, Heike Wulff, Maria Lafaille, Edward Y. Skolnik
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2010)
Article
Multidisciplinary Sciences
Xinjiang Cai, Shekhar Srivastava, Yi Sun, Zhai Li, Haiyan Wu, Ljiljana Zuvela-Jelaska, Jun Li, Rachel S. Salamon, Jonathan M. Backer, Edward Y. Skolnik
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2011)