Article
Multidisciplinary Sciences
Christophe Royer, Elizabeth Sandham, Elizabeth Slee, Falk Schneider, Christoffer B. Lagerholm, Jonathan Godwin, Nisha Veits, Holly Hathrell, Felix Zhou, Karolis Leonavicius, Jemma Garratt, Tanaya Narendra, Anna Vincent, Celine Jones, Tim Child, Kevin Coward, Chris Graham, Marco Fritzsche, Xin Lu, Shankar Srinivas
Summary: The study reveals the important role of ASPP2 in maintaining tissue integrity and cytoskeleton organization during development, particularly in regions of high apical tension. This finding has significant implications for understanding morphogenetic events in pseudostratified epithelia and tumor suppression mechanisms.
NATURE COMMUNICATIONS
(2022)
Review
Cell Biology
Philipp Oberdoerffer, Kyle M. Miller
Summary: Histone variants, such as H2A.X, H2A.Z, and macroH2A, play crucial roles in protecting the integrity of the genome through DNA damage response pathways. Their deposition, posttranslational modifications, and interactions with other proteins safeguard DNA from both endogenous and exogenous threats. Understanding the involvement of H2A variants in maintaining genome stability and their contributions to human diseases, including cancer, provides valuable insights.
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2023)
Article
Cell Biology
Gregory C. Addicks, Hongbo Zhang, Dongryeol Ryu, Goutham Vasam, Alexander E. Green, Philip L. Marshall, Sonia Patel, Baeki E. Kang, Doyoun Kim, Elena Katsyuba, Evan G. Williams, Jean-Marc Renaud, Johan Auwerx, Keir J. Menzies
Summary: Protein lysine acetylation plays a crucial role in regulating muscle integrity by inhibiting the DNA binding of the transcription factor/repressor YY1. Through acetylation-dependent inhibition of YY1, GCN5 positively regulates muscle integrity by maintaining the expression of structural muscle proteins.
JOURNAL OF CELL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Lan Yu, Yue Lang, Ching-Cheng Hsu, Wei-Min Chen, Jui-Chung Chiang, Jer-Tsong Hsieh, Michael D. Story, Zeng-Fu Shang, Benjamin P. C. Chen, Debabrata Saha
Summary: Phosphorylation of DAB2IP by Cdks leads to its interaction with PLK1 and the activation of the PLK1-Mps1 pathway, playing a critical role in SAC regulation. The phosphorylation of DAB2IP inhibits the ubiquitylation of Cdc20, blocks the premature release of APC/C-MCC, destabilizes the MCC, impairs the SAC, and increases chromosome missegregation, contributing to tumorigenesis.
Editorial Material
Biochemistry & Molecular Biology
Sharon R. Garrott, Morgan E. DeSantis
Summary: In this study, Chaaban and Carter employ cryo-electron microscopy and innovative data processing techniques to reveal the high-resolution structure of the dynein-dynactin-BICDR1 complex assembled on microtubules. The structure uncovers novel stoichiometry and offers new insights into the function and mechanism of dynein.
TRENDS IN BIOCHEMICAL SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Jack Houston, Midori Ohta, J. Sebastian Gomez-Cavazos, Amar Deep, Kevin D. Corbett, Karen Oegema, Pablo Lara-Gonzalez, Taekyung Kim, Arshad Desai
Summary: During mitosis, chromosomes connect with spindle microtubules through kinetochores and regulate mitotic progression by controlling the fate of CDC-20. This regulation involves phosphorylation and dephosphorylation processes, in which BUB-1 and PLK-1 play key roles in recruiting and phosphorylating CDC-20 to promote mitotic progression.
Article
Medicine, Research & Experimental
Qiuyu Wang, Xiaoxue Qiu, Tongyu Liu, Cheehoon Ahn, Jeffrey F. Horowitz, Jiandie D. Lin
Summary: This study reveals the importance of Tsukushi (TSK) in maintaining muscle mass, strength, endurance capacity, and muscle regeneration. TSK deficiency leads to impaired expression of myofibrillar genes, especially in slow-twitch muscle fibers, and affects neuromuscular junction formation. The effects of TSK on myofibers may be limited to certain developmental stages.
Article
Cell Biology
Abhishek Kumar, Tejashree Pradip Waingankar, Patrick D'Silva
Summary: TIM22 pathway cargos play a crucial role in maintaining mitochondrial homeostasis, but excess proteins can cause proteostatic stress and cell death. Yme1, an inner membrane metalloprotease, is involved in protein quality control and has a genetic connection with the TIM22 complex. The study reveals that impairment in the TIM22 complex can rescue the respiratory growth defects of Yme1-devoid cells, and Yme1 is essential for the stability and proteostasis of the TIM22 pathway.
JOURNAL OF CELL SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Di Ren, Zhibin He, Julia Fedorova, Jingwen Zhang, Elizabeth Wood, Xiang Zhang, David E. Kang, Ji Li
Summary: Sesn2, a stress-inducible protein, declines with aging in the heart. Lack of Sesn2 results in cardiac aging-like dysfunction and intolerance to ischemia reperfusion stress. Sesn2 deficiency impairs mitochondrial function and affects oxidative phosphorylation complex activity, impacting the maintenance of cardiac mitochondrial integrity.
Article
Cell Biology
Dhivya Kumar, Addison Rains, Vicente Herranz-Perez, Quanlong Lu, Xiaoyu Shi, Danielle L. Swaney, Erica Stevenson, Nevan J. Krogan, Bo Huang, Christopher Westlake, Jose Manuel Garcia-Verdugo, Bradley K. Yoder, Jeremy F. Reiter
Summary: A module called DISCO was discovered, which supports ciliogenesis by limiting centriole length and assembling distal appendages on the distal centriole. Lack of DISCO components CEP90 or MNR results in the inability to generate cilia and assemble distal appendages.
JOURNAL OF CELL BIOLOGY
(2021)
Editorial Material
Cell Biology
Lotte van den Goor, Ann L. Miller
Summary: This study reveals a novel interaction between tricellulin and alpha-catenin, which is essential for maintaining the integrity of the epithelial barrier.
JOURNAL OF CELL BIOLOGY
(2022)
Article
Cell Biology
L. N. Townsend, H. Clarke, D. Maddison, K. M. Jones, L. Amadio, A. Jefferson, U. Chughtai, D. M. Bis, S. Zuechner, N. D. Allen, W. van der Goes van Naters, O. M. Peters, G. A. Smith
Summary: Cdk12 acts as a negative regulator in adult neurons, maintaining axon size through the transcription of homeostatic enzymes. Loss of Cdk12 leads to elevated homocysteine, resulting in excessive actin formation and axonal swelling. Cdk12 is also necessary for long-term neuronal survival, and its loss causes age-dependent neurodegeneration, potentially linking to neurodegenerative conditions such as Alzheimer's disease.
CELL DEATH DISCOVERY
(2023)
Article
Biochemistry & Molecular Biology
Joon Park, Geoffrey K. Herrmann, Patrick G. Mitchell, Michael B. Sherman, Y. Whitney Yin
Summary: This article uses cryo-EM to discover the mechanism in which DNA polymerase gamma transitions from replication to error correction by unwinding the DNA, rotating, and backtracking to move the mismatch to the exonuclease site for proofreading.
NATURE STRUCTURAL & MOLECULAR BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Clinton K. Lau, Francis J. O'Reilly, Balaji Santhanam, Samuel E. Lacey, Juri Rappsilber, Andrew P. Carter
Summary: Dynactin is a complex that activates dynein for ultra-processive transport along microtubules. Its structure, formed by an actin-related filament, is defined by a flexible shoulder domain. By combining multiple cryo-EM datasets and precise masking strategies, the research overcame domain flexibility and obtained high-resolution maps of the Dynactin structure. The unique architecture of the shoulder securely positions subunits and reveals the molecular basis for cargo adaptor binding at the pointed end.
Article
Chemistry, Analytical
Willie H. Mims, Michael A. Temple, Robert F. Mills
Summary: The need for reliable communications in industrial systems becomes more evident as industries strive to increase reliance on automation. This paper focuses on demonstrating pre-deployment counterfeit detection using active 2D Distinct Native Attribute (2D-DNA) fingerprinting, ensuring the operational integrity of WirelessHART communications.
Article
Biology
Satoshi Takahashi, Akinori Kanai, Hiroshi Okuda, Ryo Miyamoto, Yosuke Komata, Takeshi Kawamura, Hirotaka Matsui, Toshiya Inaba, Akifumi Takaori-Kondo, Akihiko Yokoyama
Summary: Leukemic MLL fusion proteins associate with the HBO1 histone acetyltransferase complex through their THD2 domain, playing a critical role in leukemic transformation. The interaction between the HBO1 complex and MLL is a key factor in leukemic development and may serve as a potential therapeutic target for drug development.
Article
Biology
Ryo Miyamoto, Akinori Kanai, Hiroshi Okuda, Satoshi Takahashi, Hirotaka Matsui, Toshiya Inaba, Akihiko Yokoyama, Yosuke Komata
Summary: HOXA9 plays a crucial role in leukemogenesis by maintaining gene expression for anti-apoptotic pathways, particularly in synergy with MYC. Multiple anti-apoptotic pathways contribute to cooperative leukemogenesis by HOXA9 and MYC, shedding light on the high expression of HOXA9 in leukemia.
Article
Oncology
Rin Liu, Satoru Shinriki, Manabu Maeshiro, Mayumi Hirayama, Hirofumi Jono, Ryoji Yoshida, Hideki Nakayama, Hirotaka Matsui
Summary: This study found that the loss of CYLD restricted the clustering of EGFR in lipid rafts and inhibited CTX-induced apoptosis. Disruption of lipid rafts restored the endocytic function of EGFR and the activity of CTX in CYLD-downregulated cells.
Article
Cell Biology
Ravi Shankar Singh, Venkatasubramanian Vidhyasagar, Shizhuo Yang, Ananna Bhadra Arna, Manisha Yadav, Aanchal Aggarwal, Alexya N. Aguilera, Satoru Shinriki, Kalpana Kalyanasundaram Bhanumathy, Kannupriya Pandey, Aizhang Xu, Noreen Rapin, Mark Bosch, John DeCoteau, Jim Xiang, Franco J. Vizeacoumar, Yan Zhou, Vikram Misra, Hirotaka Matsui, Susan R. Ross, Yuliang Wu
Summary: The DDX41 protein plays a crucial role in the activation of the cGAS-STING pathway, which is responsible for the production of type I interferon after DNA virus infection. DDX41 regulates cGAS and STING activation, and its mutant form, R525H, shows enhanced cGAS activity. The findings suggest that DDX41 functions by unwinding and annealing DNA to maintain the balance of dsDNA and ssDNA, thus regulating cGAS-STING activation.
Article
Cardiac & Cardiovascular Systems
Hiroki Usuku, Seiji Takashio, Eiichiro Yamamoto, Toshihiro Yamada, Koichi Egashira, Mami Morioka, Masato Nishi, Takashi Komorita, Fumi Oike, Noriaki Tabata, Masanobu Ishii, Kenshi Yamanaga, Koichiro Fujisue, Daisuke Sueta, Yuichiro Arima, Satoshi Araki, Seitaro Oda, Yohei Misumi, Hiroaki Kawano, Kenichi Matsushita, Mitsuharu Ueda, Hirotaka Matsui, Kenichi Tsujita
Summary: Right ventricular global longitudinal strain (RV-GLS) provides prognostic information in patients with wild-type transthyretin amyloid cardiomyopathy (ATTRwt-CM) and has greater prognostic power than conventional echocardiographic findings.
JOURNAL OF CARDIOLOGY
(2022)
Article
Clinical Neurology
Akihiko Ueda, Makoto Nakajima, Yohei Misumi, Keiichi Nakahara, Satoru Shinriki, Masayoshi Tasaki, Hirotaka Matsui, Mitsuharu Ueda
Summary: This study found that immunohistochemical evaluation of vascular Notch3 ECD deposits in unfixed frozen skin biopsy samples may be useful for detecting Notch3 deposits in patients with CADASIL.
FRONTIERS IN NEUROLOGY
(2022)
Article
Oncology
Satoru Shinriki, Mayumi Hirayama, Akiko Nagamachi, Akihiko Yokoyama, Takeshi Kawamura, Akinori Kanai, Hidehiko Kawai, Junichi Iwakiri, Rin Liu, Manabu Maeshiro, Saruul Tungalag, Masayoshi Tasaki, Mitsuharu Ueda, Kazuhito Tomizawa, Naoyuki Kataoka, Takashi Ideue, Yutaka Suzuki, Kiyoshi Asai, Tokio Tani, Toshiya Inaba, Hirotaka Matsui
Summary: Loss of DDX41 function impairs RNA splicing and leads to DNA replication stress and R-loop formation. DDX41 binds to the splice site of coding RNA, coordinating RNA splicing and transcriptional elongation. Its loss causes splicing impairment and aberrant R-loop formation. These processes may be responsible for disease phenotypes associated with DDX41 mutations.
Review
Oncology
Satoru Shinriki, Hirotaka Matsui
Summary: Mutations in DDX41 play a role in myeloid leukemogenesis by affecting various cellular processes including RNA splicing, nucleic acid sensing, R-loop resolution, and snoRNA processing. The distinctive disease phenotype associated with DDX41 mutations suggests its involvement in the pathogenesis of myeloid malignancies.
FRONTIERS IN ONCOLOGY
(2022)
Article
Cardiac & Cardiovascular Systems
Hiroki Usuku, Eiichiro Yamamoto, Daisuke Sueta, Momoko Noguchi, Tomohiro Fujisaki, Koichi Egashira, Mami Morioka, Takashi Komorita, Fumi Oike, Koichiro Fujisue, Shinsuke Hanatani, Yuichiro Arima, Seiji Takashio, Seitaro Oda, Hiroaki Kawano, Kenichi Matsushita, Mitsuharu Ueda, Hirotaka Matsui, Kenichi Tsujita
Summary: This study investigated the significance of left ventricular apical sparing in patients with wild-type transthyretin amyloid cardiomyopathy (ATTRwt-CM). The results showed that approximately half of ATTRwt-CM patients did not exhibit left ventricular apical sparing at diagnosis. The relative apical longitudinal strain index (RapLSI) of ATTRwt-CM significantly changed over time, emphasizing the importance of repeated two-dimensional speckle tracking analysis for suspected ATTR-CM patients.
IJC HEART & VASCULATURE
(2022)
Article
Hematology
Saruul Tungalag, Satoru Shinriki, Mayumi Hirayama, Akiko Nagamachi, Akinori Kanai, Toshiya Inaba, Hirotaka Matsui
Summary: DDX41 mutation has been observed in myeloid malignancies, but the underlying mechanisms of these diseases are not fully understood. DDX41 is an ATP-dependent RNA helicase involved in ribosome biogenesis. This study investigated the role of DDX41 in translation and found that DDX41 knockdown resulted in both increased and decreased translation of certain transcripts.
INTERNATIONAL JOURNAL OF HEMATOLOGY
(2023)
Meeting Abstract
Oncology
Satoru Shinriki, Mayumi Hirayama, Akinori Kanai, Akiko Nagamachi, Toshiya Inaba, Hirotaka Matsui
Meeting Abstract
Oncology
Rin Liu, Satoru Shinriki, Manabu Maeshiro, Hirohumi Jono, Hideki Nakayama, Hirotaka Matsui
Meeting Abstract
Oncology
Manabu Maeshiro, Satoru Shinriki, Rin Liu, Yutaka Nakachi, Yoshihiro Komohara, Yukio Fujiwara, Kazuaki Otsubo, Ryoji Yoshida, Hideki Nakayama, Hirotaka Matsui
Meeting Abstract
Oncology
Akiko Nagamachi, Hirotaka Matsui, Akinori Kanai, Satoru Shinriki, Toshiya Inaba
Meeting Abstract
Oncology
Satoru Shinriki, Saruul Tungalag, Mayumi Hirayama, Akinori Kanai, Akiko Nagamachi, Toshiya Inaba, Hirotaka Matsui