Article
Biology
Beata Machnicka, Aleksander Czogalla, Dzamila M. Boguslawska, Piotr Stasiak, Aleksander F. F. Sikorski
Summary: A new missense mutation in the ANK1 gene associated with hereditary spherocytosis phenotype is reported. This mutation, L1340P substitution, affects the conformation of the ankyrin ZZUD domain important for ankyrin binding to spectrin. The study shows the impact of this mutation on protein binding activity and cell morphology.
Article
Biology
Deirdre D. Scripture-Adams, Kevin N. Chesmore, Florian Barthelemy, Richard T. Wang, Shirley Nieves-Rodriguez, Derek W. Wang, Ekaterina Mokhonova, Emilie D. Douine, Jijun Wan, Isaiah Little, Laura N. Rabichow, Stanley F. Nelson, M. Carrie Miceli
Summary: This study develops a method to isolate and sequence individual nuclei from frozen muscle, allowing for the exploration of cellular and transcriptomic mechanisms in Duchenne muscular dystrophy. The findings reveal the effects of exon skipping therapy on muscle nuclei and demonstrate partial rescue of muscle fibers, expansion of myeloid population, recovery of M2 macrophages, and repression of inflammatory fibroblasts.
COMMUNICATIONS BIOLOGY
(2022)
Article
Cell Biology
Albert C. Sek, Caroline M. Percopo, Arun K. Boddapati, Michelle Ma, Wendy E. Geslewitz, Julia O. Krumholz, Justin B. Lack, Helene F. Rosenberg
Summary: Eosinophils are no longer considered simply as cytotoxic effectors, but as complex cells with unique phenotypes that respond to stimuli in the local microenvironment. RNA sequencing revealed up-regulation of tissue remodeling genes in eosinophils from dystrophin-deficient mdx mice, and effector proteins in eosinophils from IL5tg mice without muscle lesions. Additionally, the immunomodulatory receptor Trem2 was found to be critical for eosinophil growth and differentiation, with expression correlating with donor characteristics in human peripheral blood eosinophils.
JOURNAL OF LEUKOCYTE BIOLOGY
(2021)
Article
Agriculture, Dairy & Animal Science
Nicolas Busse, Madison L. Gonzalez, Mackenzie L. Krason, Sally E. Johnson
Summary: Consumption of HMB increased the percentage of type IIA and IIA/X muscle fibers in the GM, but did not improve athletic performance in horses. Additionally, HMB supplementation did not have measurable effects on the biomechanical properties of the muscles in horses.
JOURNAL OF ANIMAL SCIENCE
(2021)
Article
Multidisciplinary Sciences
Robert Trybulski, Marta Bichowska, Rafal Piwowar, Anna Pisz, Michal Krzysztofik, Aleksandra Filip-Stachnik, Krzysztof Fostiak, Piotr Makar, Michal Wilk
Summary: The study aimed to evaluate the effects of ischemia on maximal number of repetitions, time under tension, and bar velocity during bench press exercise. The results showed that ischemia significantly decreased the time under tension. Therefore, exercising with ischemia does not enhance strength-endurance performance and bar velocity.
Review
Medicine, Research & Experimental
Colin Harper, Venkatesh Gopalan, Jorming Goh
Summary: This review examines the cellular and molecular changes in skeletal muscle mitochondria during aging, particularly focusing on the efficiency of mitochondrial coupling and its impact on muscle function decline. It also discusses how different exercise modalities can potentially reverse these changes and delay the onset of sarcopenia. Additional concepts such as mitophagy and the implications of muscle fiber type changes with sarcopenia on mitochondrial function are also integrated in this review.
JOURNAL OF TRANSLATIONAL MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Anjali Bhat, Rafay Abu, Sankarasubramanian Jagadesan, Neetha Nanoth Vellichirammal, Ved Vasishtha Pendyala, Li Yu, Tara L. Rudebush, Chittibabu Guda, Irving H. Zucker, Vikas Kumar, Lie Gao
Summary: Exercise training improves skeletal muscle health through various adaptative pathways. Nrf2, an important antioxidant transcription factor, is crucial for the adaptative responses and beneficial effects of exercise on muscle. The study suggests that Nrf2 plays a critical role in the adaptive effects of skeletal muscle and its response to exercise training.
Article
Biochemistry & Molecular Biology
Pia Apablaza, Juan Carlos Borquez, Rodrigo Mendoza, Monica Silva, Gladys Tapia, Alejandra Espinosa, Rodrigo Troncoso, Luis A. Videla, Nevenka Juretic, Andrea del Campo
Summary: Increase in body fat leads to changes in skeletal muscle and accelerates sarcopenia, known as sarco-obesity or sarcopenic obesity. Obesity affects the skeletal muscle's ability to oxidize glucose and causes mitochondrial dysfunction. Exercise improves mitochondrial dysfunction, but the effects on the mitochondrial unfolded protein response (UPRmt) in the skeletal muscle are not well understood. This study aimed to investigate the UPRmt response to exercise in obese mice and its association with skeletal muscle function improvement.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Javier Botella, Nicholas A. Jamnick, Cesare Granata, Amanda J. Genders, Enrico Perri, Tamim Jabar, Andrew Garnham, Michael Lazarou, David J. Bishop
Summary: Autophagy is an important mechanism for cells to degrade old or dysfunctional proteins and organelles. The effects of exercise on autophagosome content markers differ between rodents and humans. The decrease in LC3B-II protein levels induced by exercise in humans does not reflect a decreased autophagy flux.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Physiology
Joshua C. Drake, Rebecca J. Wilson, Di Cui, Yuntian Guan, Mondira Kundu, Mei Zhang, Zhen Yan
Summary: This study compared the roles of Ulk1 and Ulk2 in skeletal muscle insulin action following exercise training, and found that Ulk1 is essential for exercise training-induced improvement in insulin action in skeletal muscle.
FRONTIERS IN PHYSIOLOGY
(2021)
Article
Cell Biology
Elmira Ahmady, Alexandre Blais, Patrick G. Burgon
Summary: Mlip is a recently discovered protein that interacts directly with chromatin and may function as a transcriptional co-factor. In C2C12 myoblasts, blocking Mlip expression down-regulates myogenic regulatory factors and inhibits myogenic differentiation and myotube formation.
Article
Multidisciplinary Sciences
Khushwant S. Bhullar, Nan Shang, Evan Kerek, Kaiyu Wu, Jianping Wu
Summary: MOTS-c can promote mitochondrial biogenesis and fusion, but many unknown factors still exist in its mechanism of action. The study revealed a pathway in which MOTS-c promotes GLUT4 translocation by regulating mitochondrial fusion GTPases.
SCIENTIFIC REPORTS
(2021)
Article
Multidisciplinary Sciences
Joshua C. Drake, Rebecca J. Wilson, Rhianna C. Laker, Yuntian Guan, Hannah R. Spaulding, Anna S. Nichenko, Wenqing Shen, Huayu Shang, Maya Dorn, Kian Huang, Mei Zhang, Aloka B. Bandara, Matthew H. Brisendine, Jennifer A. Kashatus, Poonam R. Sharma, Alexander Young, Jitendra Gautam, Ruofan Cao, Horst Wallrabe, Paul A. Chang, Michael Wong, Eric M. Desjardins, Simon A. Hawley, George J. Christ, David F. Kashatus, Clint L. Miller, Matthew J. Wolf, Ammasi Periasamy, Gregory R. Steinberg, D. Grahame Hardie, Zhen Yan
Summary: Mitochondria form a complex, interconnected reticulum maintained through coordination among biogenesis, dynamic fission, fusion and mitophagy in response to various cues. Specific isoforms of AMP-activated protein kinase are localized on the outer mitochondrial membrane and vary in activation across the reticulum in response to energetic stress. The discovery highlights the complexity of sensing cellular energetics in vivo and its implications for targeting mitochondrial energetics in disease treatment.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Biochemistry & Molecular Biology
Angus Lindsay, Adam J. Trewin, Kate J. Sadler, Claire Laird, Paul A. Della Gatta, Aaron P. Russell
Summary: The study found that stress-resistant mdx mice show higher activity levels and better physiological function in response to behavioral stress, including greater exercise and muscle oxidative capacity, as well as less severe muscle histopathology. Female mdx mice exhibit higher activity levels following behavioral stress compared to males, a response that can be eliminated after ovariectomy but restored with estradiol.
Article
Cell Biology
Christine T. Nguyen, Majid Ebrahimi, Penney M. Gilbert, Bryan A. Stewart
Summary: This study analyzed the electrophysiological characteristics of healthy and diseased three-dimensional bioengineered skeletal muscle tissues, focusing on Duchenne muscular dystrophy. The research also tested a potential therapeutic compound and showed improvement in membrane potential in dystrophic muscle, laying the foundation for examining potential therapies for muscular disorders in vitro.
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2021)
Article
Cardiac & Cardiovascular Systems
Ines Martinez-Corral, Maria H. Ulvmar, Lukas Stanczuk, Florence Tatin, Krishnakumar Kizhatil, Simon W. M. John, Kari Alitalo, Sagrario Ortega, Taija Makinen
CIRCULATION RESEARCH
(2015)
Article
Medicine, Research & Experimental
Tomokazu Souma, Stuart W. Tompson, Benjamin R. Thomson, Owen M. Siggs, Krishnakumar Kizhatil, Shinji Yamaguchi, Liang Feng, Vachiranee Limviphuvadh, Kristina N. Whisenhunt, Sebastian Maurer-Stroh, Tammy L. Yanovitch, Luba Kalaydjieva, Dimitar N. Azmanov, Simone Finzi, Lucia Mauri, Shahrbanou Javadiyan, Emmanuelle Souzeau, Tiger Zhou, Alex W. Hewitt, Bethany Kloss, Kathryn P. Burdon, David A. MacKey, Keri F. Allen, Jonathan B. Ruddle, Sing-Hui Lim, Steve Rozen, Khanh-Nhat Tran-Viet, Xiaorong Liu, Simon John, Janey L. Wiggs, Francesca Pasutto, Jamie E. Craig, Jing Jin, Susan E. Quaggin, Terri L. Young
JOURNAL OF CLINICAL INVESTIGATION
(2016)
Article
Medicine, Research & Experimental
Benjamin R. Thomson, Tomokazu Souma, Stuart W. Tompson, Tuncer Onay, Krishnakumar Kizhatil, Owen M. Siggs, Liang Feng, Kristina N. Whisenhunt, Tammy L. Yanovitch, Luba Kalaydjieva, Dimitar N. Azmanov, Simone Finzi, Christine E. Tanna, Alex W. Hewitt, David A. Mackey, Yasmin S. Bradfield, Emmanuelle Souzeau, Shari Javadiyan, Janey L. Wiggs, Francesca Pasutto, Xiaorong Liu, Simon W. M. John, Jamie E. Craig, Jing Jin, Terri L. Young, Susan E. Quaggin
JOURNAL OF CLINICAL INVESTIGATION
(2017)
Article
Ophthalmology
Krishnakumar Kizhatil, Nina K. Sandhu, Neal S. Peachey, Vann Bennett
EXPERIMENTAL EYE RESEARCH
(2009)
Article
Multidisciplinary Sciences
Krishnakumar Kizhatil, Sheila A. Baker, Vadim Y. Arshavsky, Vann Bennett
Article
Biochemistry & Molecular Biology
Krishnakumar Kizhatil, Margaret Ryan, Jeffrey K. Marchant, Stephen Henrich, Simon W. M. John
Article
Neurosciences
Pete A. Williams, Catherine E. Braine, Krishnakumar Kizhatil, Nicole E. Foxworth, Nicholas G. Tolman, Jeffrey M. Harder, Rebecca A. Scott, Gregory L. Sousa, Alyssa Panitch, Gareth R. Howell, Simon W. M. John
MOLECULAR NEURODEGENERATION
(2019)
Article
Cell Biology
Nicholas G. Tolman, Revathi Balasubramanian, Danilo G. Macalinao, Alison L. Kearney, Katharine H. MacNicoll, Christa L. Montgomery, Wilhelmine N. de Vries, Ian J. Jackson, Sally H. Cross, Krishnakumar Kizhatil, K. Saidas Nair, Simon W. M. John
Summary: The study demonstrates that the effects of Lmx1bV265D mutations on ocular phenotypes vary significantly depending on the mouse strain background, with B6 mice being the most susceptible and 129 mice being the most resistant. A modifier locus on Chromosome 18 was identified, with the 129 allele(s) significantly lessening the severity of ocular phenotypes induced by Lmx1bV265D mutations.
DISEASE MODELS & MECHANISMS
(2021)
Article
Multidisciplinary Sciences
K. Saidas Nair, Chitrangda Srivastava, Robert V. Brown, Swanand Koli, Helene Choquet, Hong Soon Kang, Yien-Ming Kuo, Sara A. Grimm, Caleb Sutherland, Alexandra Badea, G. Allan Johnson, Yin Zhao, Jie Yin, Kyoko Okamoto, Graham Clark, Terete Borras, Gulab Zode, Krishnakumar Kizhatil, Subhabrata Chakrabarti, Simon W. M. John, Eric Jorgenson, Anton M. Jetten
Summary: Dysfunction of the trabecular meshwork (TM) is the chief cause of elevated intraocular pressure, the major risk factor of glaucoma. The authors identify the transcription factor, GLIS1, as a critical regulator of TM maintenance and intraocular pressure, and as a glaucoma risk gene.
NATURE COMMUNICATIONS
(2021)
Article
Ophthalmology
Colleen M. McDowell, Krishnakumar Kizhatil, Michael H. Elliott, Darryl R. Overby, Joseph Van Batenburg-Sherwood, J. Cameron Millar, Markus H. Kuehn, Gulab Zode, Ted S. Acott, Michael G. Anderson, Sanjoy K. Bhattacharya, Jacques A. Bertrand, Terete Borras, Diane E. Bovenkamp, Lin Cheng, John Danias, Michael Lucio De Ieso, Yiqin Du, Jennifer A. Faralli, Rudolf Fuchshofer, Preethi S. Ganapathy, Haiyan Gong, Samuel Herberg, Humberto Hernandez, Peter Humphries, Simon W. M. John, Paul L. Kaufman, Kate E. Keller, Mary J. Kelley, Ruth A. Kelly, David Krizaj, Ajay Kumar, Brian C. Leonard, Raquel L. Lieberman, Paloma Liton, Yutao Liu, Katy C. Liu, Navita N. Lopez, Weiming Mao, Timur Mavlyutov, Fiona McDonnell, Gillian J. McLellan, Philip Mzyk, Andrews Nartey, Louis R. Pasquale, Gaurang C. Patel, Padmanabhan P. Pattabiraman, Donna M. Peters, Vijaykrishna Raghunathan, Ponugoti Vasantha Rao, Naga Rayana, Urmimala Raychaudhuri, Ester Reina-Torres, Ruiyi Ren, Douglas Rhee, Uttio Roy Chowdhury, John R. Samples, E. Griffen Samples, Najam Sharif, Joel S. Schuman, Val C. Sheffield, Cooper H. Stevenson, Avinash Soundararajan, Preeti Subramanian, Chenna Kesavulu Sugali, Yang Sun, Carol B. Toris, Karen Y. Torrejon, Amir Vahabikashi, Janice A. Vranka, Ting Wang, Colin E. Willoughby, Chen Xin, Hongmin Yun, Hao F. Zhang, Michael P. Fautsch, Ernst R. Tamm, Abbot F. Clark, C. Ross Ethier, W. Daniel Stamer
Summary: This article highlights the importance of using mice as a valuable model system for studying conventional outflow resistance and intraocular pressure. It also outlines the minimum acceptable standards for developing, characterizing, and utilizing mouse models of open-angle ocular hypertension. These standards are expected to enhance scientific rigor and replicability of research findings.
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2022)
Article
Ophthalmology
Krishnakumar Kizhatil, Arthur Chlebowski, Nicholas G. Tolman, Nelson F. Freeburg, Margaret M. Ryan, Nicholas N. Shaw, Alexander D. M. Kokini, Jeffrey K. Marchant, Simon W. M. John
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2016)
Article
Biochemistry & Molecular Biology
Krishnakumar Kizhatil, Jonathan Q. Davis, Lydia Davis, Jan Hoffman, Brigid L. M. Hogan, Vann Bennett
JOURNAL OF BIOLOGICAL CHEMISTRY
(2007)
Article
Biochemistry & Molecular Biology
K Kizhatil, V Bennett
JOURNAL OF BIOLOGICAL CHEMISTRY
(2004)
Article
Cell Biology
K Kizhatil, LM Albritton
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2002)