Review
Cell Biology
Alexandre P. Garneau, Samira Slimani, Ludwig Haydock, Thy-Rene Nsimba-Batomene, Florence C. M. Prefontaine, Mathilde M. Lavoie, Laurence E. Tremblay, Marie-Jeanne Fiola, Fabrice Mac-Way, Paul Isenring
Summary: This article discusses the role of CCC in bone remodeling, highlighting its impact on the activity of Ca2+, PO42-, and H+-dependent transport systems in bone cells, as well as its association with variations in human bone density.
JOURNAL OF CELLULAR PHYSIOLOGY
(2022)
Article
Cell Biology
Egor Byvaltcev, Mahraz Behbood, Jan-Hendrik Schleimer, Thomas Gensch, Alexey Semyanov, Susanne Schreiber, Ulf Strauss
Summary: Extracellular potassium [K+]o elevation during synaptic activity retrogradely modifies presynaptic release and astrocytic uptake of glutamate. Hence, local K+ clearance and replenishment mechanisms are crucial regulators of glutamatergic transmission and plasticity. Based on recordings of astrocytic inward rectifier potassium current IKir and K+-sensitive electrodes as sensors of [K+]o as well as on in silico modeling, we demonstrate that the neuronal K+-Cl- co-transporter KCC2 clears local perisynaptic [K+]o during synaptic excitation by operating in an activity-dependent reversed mode.
Article
Neurosciences
Ru Liu, Yue Xing, Herui Zhang, Junling Wang, Huanling Lai, Lipeng Cheng, Donghong Li, Tao Yu, Xiaoming Yan, Cuiping Xu, Yueshan Piao, Linghui Zeng, Horace H. Loh, Guojun Zhang, Xiaofeng Yang
Summary: Imbalanced expression of NKCC1 and KCC2 has been implicated in the pathogenesis of epilepsy. This study investigated the effects of this imbalance on GABAergic neurotransmission in certain brain regions. The results showed depolarized GABAergic signals in pyramidal neurons, and the specific NKCC1 inhibitor bumetanide effectively dampened these signals. Additionally, FCD patients exhibited altered expression and distribution of KCC2.
FRONTIERS IN MOLECULAR NEUROSCIENCE
(2022)
Review
Chemistry, Multidisciplinary
Erin McMoneagle, Jin Zhou, Shiyao Zhang, Weixue Huang, Sunday Solomon Josiah, Ke Ding, Yun Wang, Jinwei Zhang
Summary: Epilepsy is a neurological disorder characterized by seizures, and impaired function of the KCC2 protein has been found to contribute to its pathogenesis. Enhancing KCC2 activation and regulating its phosphorylation and transcription have shown promising results in attenuating seizure severity. Recent advances in understanding the regulatory mechanisms of KCC2 have led to the discovery of novel small molecules that modulate its activity, making it a potential therapeutic target for treating epilepsy.
ACTA PHARMACOLOGICA SINICA
(2023)
Article
Physiology
Celine Caravagna, Alexis Casciato, Jacques-Olivier Coq, Sylvie Liabeuf, Cecile Brocard, Julie Peyronnet, Laurence Bodineau, Florence Cayetanot
Summary: Prenatal hypoxia disrupts the development of Cl- cotransporters KCC2 and NKCC1, leading to alterations in central respiratory drive. This disruption may have long-term effects on the functioning of GABA(A) and glycinergic systems.
FRONTIERS IN PHYSIOLOGY
(2022)
Review
Cell Biology
Andree-Anne Marcoux, Laurence E. Tremblay, Samira Slimani, Marie-Jeanne Fiola, Fabrice Mac-Way, Alexandre P. Garneau, Paul Isenring
Summary: NKCC2 is an integral membrane protein that mediates the cotranslocation of Na+, K+, and Cl(-) ions in the TALH, allowing for the reclamation of a large fraction of filtered Na+, Cl-, Ca2+, Mg-2(+), and HCO(3)(-). Malfunction of NKCC2 can lead to various inherited disorders, illustrating its physiological importance.
JOURNAL OF CELLULAR PHYSIOLOGY
(2021)
Article
Endocrinology & Metabolism
Lin Zhao, ZhiMao Li, Xu Meng, Hua Fan, ZengLei Zhang, ZhaoCai Zhang, YeCheng Liu, XianLiang Zhou, HuaDong Zhu
Summary: This study found significant differences in clinical characteristics between large and small pheochromocytomas and paragangliomas (PPGLs). Male patients were more likely to have large tumors, and large tumors were more likely to be located on the adrenal glands. Catecholamine measurements also helped predict tumor size of PPGLs.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Article
Transportation Science & Technology
Elham Saffari, Mehmet Yildirimoglu, Mark Hickman
Summary: This study aims to estimate the MFD for a large-scale urban network by combining probe vehicle data with an unknown penetration rate and full-scale approximate traffic data based on loop detector data. The Bayesian fusion method outperforms the baseline method in average flow and density estimations, especially showing significant improvement in average density estimations.
TRANSPORTATION RESEARCH PART C-EMERGING TECHNOLOGIES
(2022)
Article
Engineering, Marine
Patrichka Wei-Yi Chen, Madeline Olivia, Wen-Chen Chou, Ruei-Feng Shiu, Vladimir Mukhanov, An-Yi Tsai
Summary: Seagrass meadows are crucial ecosystems with microbial loop playing a key role in transferring organic matter to aquatic food chains. Bacterial growth rate is higher in seagrass habitats, while bacterial production in unvegetated waters is influenced by resource availability.
JOURNAL OF MARINE SCIENCE AND ENGINEERING
(2023)
Review
Cell Biology
Xuebing Xu, Tong Wu, Renjie Lin, Shengze Zhu, Jie Ji, Dandan Jin, Mengxiang Huang, Wenjie Zheng, Wenkai Ni, Feng Jiang, Shihai Xuan, Mingbing Xiao
Summary: The migrasome is a newly discovered organelle that appears when cells migrate. It is detached from the cell and released into the extracellular space or absorbed by other cells. The migrasome functions through a release mechanism called migracytosis, which depends on cell migration and transports cytoplasmic contents. This review highlights the differences between migrasomes and exosomes in terms of size, composition, function, extraction method, and regulation mechanism, while also discussing the prospects and potential clinical applications of migrasomes.
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
(2023)
Article
Biochemistry & Molecular Biology
Laura Otero-Ortega, Elisa Alonso-Lopez, Maria Perez-Mato, Fernando Laso-Garcia, Mari Carmen Gomez-de Frutos, Luke Diekhorst, Maria Laura Garcia-Bermejo, Elisa Conde-Moreno, Blanca Fuentes, Maria Alonso de Lecinana, Eduardo Armada, Lorena Buiza-Palomino, Exuperio Diez-Tejedor, Maria Gutierrez-Fernandez
Summary: The study analyzed the content of circulating EVs in patients with IS and MI, finding common proteins and miRNAs as well as differences between the two groups. The research suggests a relationship between circulating EV content and the patient's disease state, with certain proteins and miRNAs participating in both diseases while others are specific to IS patients.
Article
Neurosciences
Jiang-Jian Hu, Yuexin Liu, Hongyu Yao, Boxu Cao, Huabao Liao, Ruodi Yang, Peng Chen, Xue-Jun Song
Summary: The rapid downregulation of KCC2 in the VPM is found to be a common mechanism for the brain to regain consciousness from anesthesia in mice. This downregulation is driven by ubiquitin-proteasomal degradation mediated by Fbxl4. Phosphorylation of KCC2 at Thr1007 facilitates the interaction between KCC2 and Fbxl4, leading to GABA(A) receptor-mediated disinhibition and accelerated recovery of VPM neuron excitability and consciousness.
NATURE NEUROSCIENCE
(2023)
Article
Chemistry, Analytical
Yizhe Huang, Bin Huang, Zhifu Zhang, Yuanyuan Shi, Yizhao Yuan, Jinfeng Sun
Summary: This article proposes a loop closure detection method based on the similarity differences between image blocks. By using a lightweight convolutional neural network to extract image descriptors and calculating the similarity among blocks, the accuracy of loop closure detection is improved.
Review
Biotechnology & Applied Microbiology
Victor Alfonso Solarte David, Viviana Raquel Guiza-Argueello, Martha L. Arango-Rodriguez, Claudia L. Sossa, Silvia M. Becerra-Bayona
Summary: The absence or damage of tissue is a major cause of diseases, and novel therapeutic alternatives aim to recover lost functions through tissue regeneration. Chronic cutaneous lesions are common wounds that require regenerative medicine and tissue engineering interventions to develop bioactive medical products for proper healing and prevention of complications. The coordination of cells, extracellular matrix (ECM), and biomolecules is crucial in tissue repair and regeneration, with ECM acting as a biological platform for their interactions. Tissue engineering utilizes synthetic polymers and decellularized tissues to mimic native ECM and provide necessary functionalities for cell differentiation.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Biodiversity Conservation
Joseph E. Carrara, Christopher A. Walter, Zachary B. Freedman, Ashley N. Hostetler, Jennifer S. Hawkins, Ivan J. Fernandez, Edward R. Brzostek
Summary: As nitrogen deposition increases, forest soils dominated by trees that associate with ectomycorrhizal fungi store more carbon compared to those dominated by trees associating with arbuscular mycorrhizae. This difference is likely due to unique nutrient cycling responses to nitrogen between the two types of mycorrhizal soils.
GLOBAL CHANGE BIOLOGY
(2021)
Meeting Abstract
Biochemistry & Molecular Biology
Cristina Marcelo, Adriana Mercado, Paola de los Heros
Article
Multidisciplinary Sciences
Jinwei Zhang, Antje Cordshagen, Igor Medina, Hans Gerd Nothwang, Jacek R. Wisniewski, Michael Winklhofer, Anna-Maria Hartmann
Article
Cell Biology
Diana Pacheco-Alvarez, Diego Luis Carrillo-Perez, Adriana Mercado, Karla Leyva-Rios, Erika Moreno, Elisa Hernandez-Mercado, Maria Castaneda-Bueno, Norma Vazquez, Gerardo Gamba
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2020)
Article
Audiology & Speech-Language Pathology
Maike Claussen, Jennifer Schulze, Hans Gerd Nothwang
Article
Cell Biology
Brandon E. McFarlin, Yuhan Chen, Taylor S. Priver, Donna L. Ralph, Adriana Mercado, Gerardo Gamba, Meena S. Madhur, Alicia A. McDonough
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2020)
Article
Biochemistry & Molecular Biology
Lilia G. Noriega, Zesergio Melo, Renuga D. Rajaram, Adriana Mercado, Armando R. Tovar, Laura A. Velazquez-Villegas, Maria Castaneda-Bueno, Yazmin Reyes-Lopez, Dongryeol Ryu, Lorena Rojas-Vega, German Magana-Avila, Adriana M. Lopez-Barradas, Mariana Sanchez-Hernandez, Anne Debonneville, Alain Doucet, Lydie Cheval, Nimbe Torres, Johan Auwerx, Olivier Staub, Gerardo Gamba
Summary: SIRT7 interacts with, stabilizes, and modulates the activity of KCC4 through deacetylation, revealing a novel role for SIRT7 in renal physiology.
Article
Biochemistry & Molecular Biology
Anna-Maria Hartmann, Lifei Fu, Christine Ziegler, Michael Winklhofer, Hans Gerd Nothwang
Summary: KCCs exhibit significant variations in K+ affinities, with conserved K+- and Cl--binding sites. The structural modification of EL2 may be an elegant way to modulate the K+ affinity of the different isoforms in the KCC subfamily.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Article
Neurosciences
Friedrich Schinzel, Hannah Seyfer, Lena Ebbers, Hans Gerd Nothwang
Summary: The dorsal cochlear nucleus (DCN) and cerebellum share genetic programs, with Lbx1 being a key component. Different cell types in the DCN and cerebellum were found to express Lbx1, contributing to the genetic individuation to adapt to specific tasks.
JOURNAL OF COMPARATIVE NEUROLOGY
(2021)
Article
Neurosciences
Ali Jason Saleh, Hans Gerd Nothwang
Summary: A comparative study of miRNA expression during development of the auditory hindbrain in mammals and chickens revealed both shared and distinct features. In the nucleus magnocellularis, differential expression patterns along the cochleotopic axis were observed, with miRNAs reflecting different rhombomeric origins. Bioinformatics analysis predicted mRNA targets associated with neuronal developmental processes. Overall, this study adds miRNA patterns to the list of cochleotopic and developmental gradients in the central auditory system.
JOURNAL OF COMPARATIVE NEUROLOGY
(2021)
Article
Neurosciences
Constanze Krohs, Christoph Koerber, Lena Ebbers, Faiza Altaf, Giulia Hollje, Simone Hoppe, Yvette Doerflinger, Haydn M. Prosser, Hans Gerd Nothwang
Summary: A point mutation in miR-96 leads to hearing loss and alters the structure of the central auditory system. Mir-183/96 gene cluster deletion in mice results in reduced volumes of auditory hindbrain nuclei and changes in synaptic transmission. These genetic alterations impact synaptic functions and morphology in the auditory hindbrain.
JOURNAL OF NEUROSCIENCE
(2021)
Article
Nutrition & Dietetics
Claudia Tovar-Palacio, Lilia G. Noriega, Adriana Mercado
Summary: SIRT1 and NAD(+) metabolism play important roles in renal diseases. Polyphenols derived from natural sources can activate SIRT1 and regulate NAD(+) metabolism, thus preventing and treating various diseases.
Article
Biochemistry & Molecular Biology
Roxana Perez-Villavicencio, Javier Flores-Estrada, Martha Franco, Bruno Escalante, Oscar Perez-Mendez, Adriana Mercado, Rocio Bautista-Perez
Summary: This study investigated the effect of empagliflozin on sphingomyelin and its metabolites in diabetic and hypertensive rats. The results showed that empagliflozin decreased hyperglycemia in diabetic rats and reduced the levels of sphingomyelin and related substances. It also regulated the activity of certain enzymes. In hypertensive rats, empagliflozin mainly decreased the levels of sphingomyelin in the catabolic pathway.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Neurosciences
Nicolas I. C. Muller, Isabelle Paulussen, Lina N. Hofmann, Jonas O. Fisch, Abhyudai Singh, Eckhard Friauf
Summary: Sound localization involves information analysis in the lateral superior olive (LSO), a conspicuous nucleus in the mammalian auditory brainstem. This study shows that acoustic experience plays a significant role in the development of reliable neurotransmission in the LSO, which is essential for effective interaural level difference (ILD) detection.
JOURNAL OF PHYSIOLOGY-LONDON
(2022)
Review
Cell Biology
Fatima Trejo, Stephanie Elizalde, Adriana Mercado, Gerardo Gamba, Paola de losHeros
Summary: The aim of this review is to analyze the cryo-EM data of the SLC12A superfamily of membrane transporters and their structural elements, as well as their role in physiological processes and pathophysiological conditions. The review also discusses the potential of using these structural elements for the development of novel treatments and to gain mechanistic insight into cation-coupled chloride cotransporters.
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2023)
Article
Cell Biology
Constanze Krohs, Mor Bordeynik-Cohen, Naama Messika-Gold, Ran Elkon, Karen B. Avraham, Hans Gerd Nothwang
Summary: The study reveals that mammalian peripheral and central auditory systems share critical genes, including 12 cochlea-abundant miRNAs, relevant for proper development and function. This has implications for auditory rehabilitation and the evolution of the auditory system.
CELL AND TISSUE RESEARCH
(2021)