Article
Microbiology
Momen Askoura, Ahmad J. Almalki, Amr S. Abu Lila, Khaled Almansour, Farhan Alshammari, El-Sayed Khafagy, Tarek S. Ibrahim, Wael A. H. Hegazy
Summary: The CRISPR-Cas9 system was used to target the S. enterica sdiA gene, showing significant impacts on biofilm formation, cell adhesion, and invasion, but not intracellular survival. Double mutations in sdiA and ssaV attenuated S. enterica virulence and in vivo pathogenesis. Mutations in selected genes increased Salmonella susceptibility to antibiotics.
Review
Microbiology
Amber J. Barton, Jennifer Hill, Christoph J. Blohmke, Andrew J. Pollard
Summary: This review summarizes the advances in understanding the pathogenesis of enteric fever, including mechanisms of host restriction, intestinal invasion, interactions with innate immunity, and chronic carriage, and discusses how this knowledge may advance future vaccines and antimicrobials.
FEMS MICROBIOLOGY REVIEWS
(2021)
Review
Microbiology
Amber J. Barton, Jennifer Hill, Christoph J. Blohmke, Andrew J. Pollard
Summary: The lack of vaccines against paratyphoid, multidrug resistance, and chronic carriage pose challenges for enteric fever elimination, despite the recent recommendation of conjugate vaccines for typhoid fever by WHO. Advances in in vitro and human challenge models in the past decade have enhanced understanding of enteric fever pathogenesis and have the potential to drive future developments in vaccines and antimicrobials.
FEMS MICROBIOLOGY REVIEWS
(2021)
Article
Microbiology
Hong Li, Xiang Ma, Yanqiong Tang, Dan Wang, Ziding Zhang, Zhu Liu
Summary: This study constructed a genome-wide PPI network for Aeromonas veronii and found that its virulence factors had higher degree and betweenness centrality, tended to interact with each other, and were enriched in specific modules. The virulence factors may regulate A. veronii's virulence through signal transduction pathways and manipulate host biological processes by mimicking and competitively binding to host proteins.
Article
Biology
Hina Khan, Partha Paul, Ritesh Rajesh Sevalkar, Sangita Kachhap, Balvinder Singh, Dibyendu Sarkar
Summary: This study identifies a new set of genes regulated by cyclic AMP (cAMP) in Mycobacterium tuberculosis, including virulence factors controlled by the major regulator PhoP. The results show that PhoP, as a DNA binding transcription factor, impacts gene expression and phosphorylated PhoP promotes recruitment of cAMP receptor protein (CRP) at target promoters. Additionally, a novel regulatory mechanism is uncovered, where activation of these genes requires direct recruitment of both PhoP and CRP at their target promoters.
Review
Biochemistry & Molecular Biology
Jun Chen, Ailin Xiong, Yuhao Ma, Chenghe Qin, Chun Loong Ho
Summary: The microbiome, a collection of microbial genomes in a specific environment, has a significant impact on host bone health. Disruption in the host-microbiome interaction can lead to conditions like osteomyelitis and other bone diseases, indicating the importance of understanding the role of microbiota in bone health.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2021)
Article
Microbiology
Susan M. Brewer, Christian Twittenhoff, Jens Kortmann, Sky W. Brubaker, Jared Honeycutt, Liliana Moura Massis, Trung H. M. Pham, Franz Narberhaus, Denise M. Monack
Summary: Bacterial pathogens adjust their virulence factor expression in response to temperature changes through an RNA thermosensor, impacting their survival and infection capability. Specific pathogens like Salmonella enterica serovar Typhi (S. Typhi) regulate gene expression to successfully infect hosts and evade immune responses.
Article
Biochemistry & Molecular Biology
Anastasiya Merkushova, Anton Shikov, Anton Nizhnikov, Kirill Antonets
Summary: This study conducted a comprehensive pangenome analysis of Salmonella enterica and identified potential loci determining host specificity. The study revealed high genetic diversity in the pangenome and identified multiple genes potentially associated with host specificity. Additionally, antibiotic resistance loci were found in host-adapted strains.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Nana Liu, Ping Wang, Xiancai Li, Yakun Pei, Yun Sun, Xiaowen Ma, Xiaoyang Ge, Yutao Zhu, Fuguang Li, Yuxia Hou
Summary: This study identifies lncRNAs involved in the regulation of pathogenesis-related genes in Verticillium dahliae, a fungus causing vascular wilt disease in cotton. It uncovers the regulatory role of lncRNAs in filamentous fungi for the first time. These findings advance our understanding of the development and pathogenesis of V. dahliae and offer alternatives in controlling diseases caused by fungal attack.
Article
Microbiology
Abrar K. Thabit, Khalid Eljaaly, Ayat Zawawi, Tarek S. Ibrahim, Ahmed G. Eissa, Samar S. Elbaramawi, Wael A. H. Hegazy, Mahmoud A. Elfaky
Summary: The study found that atenolol can effectively reduce the biofilm formation, invasion, and replication of Salmonella Typhimurium. Atenolol affects the virulence of Salmonella by downregulating related genes and protects mice from infection. In silico molecular docking study showed that atenolol can compete with the target proteins of Salmonella.
Review
Cell Biology
Siva R. Uppalapati, Andres Vazquez-Torres
Summary: The metal ion manganese (Mn2+) is important for both hosts and bacterial pathogens. Hosts restrict Mn2+ to combat bacterial infection, while pathogens have mechanisms to counteract this restriction. Mn2+ serves as a cofactor in antioxidant defense and central metabolism. Bacterial pathogens prefer Mn2+-cofactored enzymes during oxidative stress. Mn2+ catalyzes metabolic pathways that provide outputs related to Salmonella's resistance against reactive oxygen species generated during the respiratory burst.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Review
Microbiology
Barbara M. Schultz, Felipe Melo-Gonzalez, Geraldyne A. Salazar, Barbara N. Porto, Claudia A. Riedel, Alexis M. Kalergis, Susan M. Bueno
Summary: Salmonella enterica is a common source of food and water-borne infections, causing a wide range of clinical ailments in both human and animal hosts. Immunity to Salmonella involves an interplay between different immune responses, despite the bacterium's strategies to evade host immune responses.
FRONTIERS IN MICROBIOLOGY
(2021)
Review
Immunology
Rachel A. Cheng, Martin Wiedmann
Summary: Salmonella enterica is a diverse pathogen with over 2600 serovars, utilizing fimbriae as important organelles for interaction with hosts. Recent genome sequencing has uncovered novel fimbriae, shedding light on their diversity and distribution. Understanding Salmonella chaperone-usher fimbriome evolution and complexities in host-pathogen interactions is crucial for future research.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Fisheries
Mengru Jin, Jiaojiao He, Jun Li, Yonghua Hu, Dongmei Sun, Hanjie Gu
Summary: The study reveals that YccA is a novel virulence factor of E. piscicida and essential for bacterial pathogenicity through evasion of the host's innate immune functions.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Review
Biotechnology & Applied Microbiology
Leon Kemper, Andreas Hensel
Summary: Campylobacter jejuni, causing severe enteritis, has unique features and adapts effectively to environmental conditions. The review summarizes the strategies of C. jejuni for host-pathogen interaction, providing insights for future drug development.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Review
Microbiology
Clea Melenotte, Soraya Mezouar, Jean-Louis Mege, Jean-Pierre Gorvel, Guido Kroemer, Didier Raoult
CRITICAL REVIEWS IN MICROBIOLOGY
(2020)
Article
Cell Biology
Lucrecia Alberdi, Alexandra Vergnes, Jean-Baptiste Manneville, Dumizulu L. Tembo, Ziyan Fang, Yaya Zhao, Nina Schroeder, Audrey Dumont, Margaux Lagier, Patricia Bassereau, Lorena Redondo-Morata, Jean-Pierre Gorvel, Stephane Meresse
JOURNAL OF CELL SCIENCE
(2020)
Article
Biochemistry & Molecular Biology
Semeli Platsaki, Xin Zhou, Berangere Pinan-Lucarre, Vincent Delauzun, Haijun Tu, Pascal Mansuelle, Patrick Fourquet, Yves Bourne, Jean-Louis Bessereau, Pascale Marchot
JOURNAL OF BIOLOGICAL CHEMISTRY
(2020)
Article
Biochemistry & Molecular Biology
Rebecca Young, Dylan E. Jones, Lautaro Diacovich, Andrzej Witkowski, Robert O. Ryan
Summary: An antibody-based method was used to detect and quantify 3-methylglutaconic aciduria, showing recognition of 3MGC-conjugated proteins by immune serum. Unexpectedly, trans-3MGC acid isomerized to cis-3MGC acid after conjugation, leading to the detection of 3MGCylated proteins. This non-enzymatic isomerization reaction could explain the presence of cis-3MGC acid in urine of individuals with 3MGC aciduria.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Microbiology
M. R. Tuttobene, J. F. Perez, E. Pavesi, Perez Mora B, D. Biancotti, P. Cribb, M. Altilio, G. Mueller, H. Gramajo, G. Tamagno, M. S. Ramirez, L. Diacovich, M. A. Mussi
Summary: Light can modulate important pathogenicity determinants and virulence in ESKAPE priority pathogens found in warm-blooded hosts, such as Staphylococcus aureus, Pseudomonas aeruginosa, and Acinetobacter spp., highlighting the potential use of light to control pathogen's virulence. Moreover, the response to light varies among different species, with virulence being enhanced in some species and reduced in others in an epithelial infection model.
JOURNAL OF BACTERIOLOGY
(2021)
Review
Neurosciences
Davide Comoletti, Laura Trobiani, Arnaud Chatonnet, Yves Bourne, Pascale Marchot
Summary: Cell adhesion involves the formation of protein complexes between two cells, with neural cell-adhesion proteins using cholinesterase-like domains to bind cognate partners. These proteins, including neuroligins, have unique structural features but share the ability to recognize and bind protein partners.
Article
Multidisciplinary Sciences
Thanida Laopanupong, Pinidphon Prombutara, Phongthon Kanjanasirirat, Salisa Benjaskulluecha, Atsadang Boonmee, Tanapat Palaga, Stephane Meresse, Jiraporn Paha, Tegar Adriansyah Putra Siregar, Tanawadee Khumpanied, Suparerk Borwornpinyo, Angkana Chaiprasert, Pongsak Utaisincharoen, Marisa Ponpuak
Summary: This study showed that specific genes such as Kxd1 and Plekhm2 are upregulated in macrophages infected with autophagy-resistant Beijing strain of Mycobacterium tuberculosis, compared to those infected with the reference strain H37Rv, leading to suppression of lysosome positioning and delivery during starvation-induced autophagy. These genes play a crucial role in allowing the BJN strain to evade autophagic restriction and survive within host cells.
SCIENTIFIC REPORTS
(2021)
Article
Multidisciplinary Sciences
Marisel Romina Tuttobene, Gabriela Leticia Mueller, Lucia Blasco, Natalia Arana, Monica Hourcade, Lautaro Diacovich, Pamela Cribb, Maria Tomas, Carlos Gabriel Nieto-Penalver, Maria Alejandra Mussi
Summary: The study demonstrates that light significantly modulates the regulatory mechanism of collective behaviors in Acinetobacter baumannii, affecting the production levels of acyl-homoserine lactones (AHLs) through interactions involving proteins such as AbaI and BlsA.
SCIENTIFIC REPORTS
(2021)
Article
Cell Biology
Ziyan Fang, Mathieu Fallet, Thomas Moest, Jean-Pierre Gorvel, Stephane Meresse
Summary: In this study, it was found that the microtubule molecular motor KIF1B beta plays a key role in the establishment of the Salmonella replication niche, and its absence affects the stability of bacterial vacuoles and the formation of associated tubules. Kinesin-3 interacts with the Salmonella effector SifA and the host protein complex SKIP. The presence of SifA establishes a recruitment pathway for kinesin-1 and kinesin-3 in infected cells.
JOURNAL OF CELL SCIENCE
(2022)
Article
Multidisciplinary Sciences
S. Holbert, E. Barilleau, S. M. Roche, J. Trotereau, S. Georgeault, J. Burlaud-Gaillard, A. Wiedemann, S. Meresse, I Virlogeux-Payant, P. Velge
Summary: Numerous studies have shown that the Salmonella Pathogenicity Island 1-encoded type III secretion system (T3SS1) apparatus and its associated effectors play a key role in the invasion and intracellular fate of Salmonella in host cells. Some cell lines have been identified where Salmonella Typhimurium can enter without using its three known invasion factors. In the murine AML12 hepatocyte cell line, the T3SS1 mutant induced an intracellular fate similar to the wild-type strain, indicating that invasion and survival of Salmonella Typhimurium can be independent of T3SS1.
SCIENTIFIC REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Veronique Roig-Zamboni, Sarah Barelier, Robert Dixon, Nicola F. Galley, Amani Ghanem, Heloize Cahuzac, Quoc Phong Nguyen, Bartlomiej Salamaga, Peter J. Davis, Yves Bourne, Stephane Mesnage, Florence Vincent
Summary: The cleavage of septal peptidoglycan during cell division helps in separating the daughter cells. The N-acetylglucosaminidase AtlA in Enterococcus faecalis plays a crucial role in cell separation and its mutants show decreased virulence. AtlA has structural homologs in other pathogens, making it a potential target for developing inhibitors of bacterial pathogenesis.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Firas Fadel, Violla Bassim, Vanessa Francis, Steven L. Porter, Thomas Botzanowski, Pierre Legrand, Maria Mate Perez, Yves Bourne, Sarah Cianferani, Florence Vincent
Summary: This study provides structural and functional insights into the interaction between GacS and RetS in Pseudomonas aeruginosa, including the signaling pathway and autokinase mechanism of GacS. The findings are of great significance for a better understanding of these regulatory networks and their roles in bacterial physiological activities.
Article
Biochemistry & Molecular Biology
Sarah Barelier, Romain Avellan, Giri Raj Gnawali, Patrick Fourquet, Veronique Roig-Zamboni, Isabelle Poncin, Vanessa Point, Yves Bourne, Stephane Audebert, Luc Camoin, Christopher D. Spilling, Stephane Canaan, Jean-Francois Cavalier, Gerlind Sulzenbacher
Summary: We report the synthesis of new CyC alkyne-containing inhibitors (CyCyne) and their use for the direct fishing of target proteins in M. tb culture via bio-orthogonal click-chemistry activity-based protein profiling (CC-ABPP). This approach led to the capture and identification of a variety of enzymes, including HsaD, which is required for the survival of M. tb within macrophages and is a potential therapeutic target. The specificity of HsaD inhibition by the CyC analogues was confirmed through biochemical and structural approaches.
Meeting Abstract
Biochemistry & Molecular Biology
P. Marchot, Y. Bourne