期刊
JOURNAL OF CELL SCIENCE
卷 133, 期 9, 页码 -出版社
COMPANY BIOLOGISTS LTD
DOI: 10.1242/jcs.239863
关键词
Effector protein; Infection; Kinesin-1; PipB2; Salmonella; SifA; Membrane tubules
类别
资金
- China Scholarship Council
- Institut National de la Sante et de la Recherche Medicale, Centre National de la Recherche Scientifique, Aix-Marseille University
- Agence Nationale de la Recherche [ANR-11-LABX-0054, ANR-10-INBS-04, ANR-16-CE15-0023-01]
- Agence Nationale de la Recherche (ANR) [ANR-16-CE15-0023] Funding Source: Agence Nationale de la Recherche (ANR)
Salmonella enterica is an intracellular bacterial pathogen. The formation of its replication niche, which is composed of a vacuole associated with a network of membrane tubules, depends on the secretion of a set of bacterial effector proteins whose activities deeply modify the functions of the eukaryotic host cell. By recruiting and regulating the activity of the kinesin-1 molecular motor, Salmonella effectors PipB2 and SifA play an essential role in the formation of the bacterial compartments. In particular, they allow the formation of tubules from the vacuole and their extension along the microtubule cytoskeleton, and thus promote membrane exchanges and nutrient supply. We have developed in vitro and in cellulo assays to better understand the specific role played by these two effectors in the recruitment and regulation of kinesin-1. Our results reveal a specific interaction between the two effectors and indicate that, contrary to what studies on infected cells suggested, interaction with PipB2 is sufficient to relieve the autoinhibition of kinesin-1. Finally, they suggest the involvement of other Salmonella effectors in the control of the activity of this molecular motor. This article has an associated First Person interview with the first author of the paper.
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