Article
Multidisciplinary Sciences
Yanyan Chen, Yan Wang, Juan Chen, Wu Zuo, Yong Fan, Sijia Huang, Yongmei Liu, Guangming Chen, Qing Li, Jinsong Li, Jian Wu, Qian Bian, Chenhui Huang, Ming Lei
Summary: The interaction between telomeres and the LINC complex, specifically between SUN1 and SPDYA, is crucial for homologous pairing during meiosis prophase I. This interaction is essential for the formation of a ring-shaped telomere supramolecular architecture at the nuclear envelope, which is critical for efficient meiotic progression.
NATURE COMMUNICATIONS
(2021)
Review
Cell Biology
Juane Lu, Tao Wu, Biao Zhang, Suke Liu, Wenjun Song, Jianjun Qiao, Haihua Ruan
Summary: This article summarizes the types of nuclear localization signals, focuses on recently reported proteins containing such signals, and briefly outlines mechanisms for nuclear entry that do not rely on nuclear localization signals.
CELL COMMUNICATION AND SIGNALING
(2021)
Article
Virology
Luis Adrian De Jesus-Gonzalez, Selvin Noe Palacios-Rapalo, Jose Manuel Reyes-Ruiz, Juan Fidel Osuna-Ramos, Carlos Noe Farfan-Morales, Carlos Daniel Cordero-Rivera, Bulmaro Cisneros, Ana Lorena Gutierrez-Escolano, Rosa Maria del Angel
Summary: Recent evidence suggests that nuclear elements play a role in the replication of Flaviviruses, with certain viral proteins being imported into the nucleus. Furthermore, nuclear localization of these proteins has been observed in infected cells. The presence of ZIKV NS3 in the nucleus has been investigated, showing that NS3 is imported into the nucleus via the importin pathway and exported back to the cytoplasm.
JOURNAL OF VIROLOGY
(2023)
Article
Biology
Po Jui Chen, Anna B. McMullin, Bryan J. Visser, Qian Mei, Susan M. Rosenberg, David Bates
Summary: In bacteria, sister replisomes are functionally interdependent and remain colocalized throughout replication. Our study reveals the crucial role of physical association between sister replisomes in ensuring an efficient and uninterrupted replication program.
Article
Biochemistry & Molecular Biology
Min Chen, Xue Yang, Haiyang Liu, Jun Wan
Summary: In this study, a functional bipartite nuclear localization signal (bNLS) in the ANKRD11 gene was identified. It was found that this bNLS is responsible for the nuclear import of ANKRD11 and certain clinical variants of this signal may be related to disease development.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2023)
Article
Plant Sciences
Jie Wu, Ying Song, Zi-Sheng Zhang, Jing-Xian Wang, Xuan Zhang, Jian-Ye Zang, Ming-Yi Bai, Lin-Hui Yu, Cheng-Bin Xiang
Summary: This study reveals the critical role of the GAF domain of AtNLP7 in perceiving nitrate deficiency signals and mediating nitrate-triggered ROS signaling. The GAF domain is involved in the transport and activation of AtNLP7 in response to nitrate deficiency, as well as in the regulation of ROS accumulation.
Article
Biochemistry & Molecular Biology
Athanasios A. Panagiotopoulos, Chara Polioudaki, Sotirios G. Ntallis, Dimitris Dellis, George Notas, Christos A. Panagiotidis, Panayiotis A. Theodoropoulos, Elias Castanas, Marilena Kampa
Summary: The study identified a novel NLS signal sequence for importin 7 recognition using unsupervised computational methods and experimental validation, showcasing its effectiveness in breast cancer cells. Furthermore, it was found that importin 7-mediated nuclear protein transport is affected by cargo protein phosphorylation.
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2021)
Letter
Oncology
Hu Lei, Li Yang, Yingying Wang, Zhihui Zou, Meng Liu, Hanzhang Xu, Yingli Wu
Summary: JOSD2 blocks the nuclear localization of PKM2 by reducing its K433 acetylation, thereby reducing AML progression.
EXPERIMENTAL HEMATOLOGY & ONCOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Susana Mendez-Gomez, Heidi Espadas-Alvarez, Ivette Ramirez-Rodriguez, Lilianha Dominguez-Malfavon, Refugio Garcia-Villegas
Summary: The localization of TRPV4 ion channel between the nucleus and plasma membrane is crucial for its cellular functions, and mutations may lead to TRPV4 channelopathies.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Oncology
Huijuan Liu, Jiahuan Yang, Yang Zhang, Jingxia Han, Yuyan Yang, Zihan Zhao, Xintong Dai, Hongqi Wang, Xiujuan Ding, Yanrong Liu, Weilong Zhong, Wenqing Gao, Tao Sun
Summary: This study confirmed the impact of psychological stress on tumor progression and identified DRD2 as a potential mediator of stress-induced malignant tumor progression. The results suggest that DRD2 regulation of HIF1 alpha may play a key role in this process, and DRD2 inhibition with TFP could be a promising therapeutic strategy for managing stress in cancer patients.
Article
Cell Biology
Maksim M. M. Karasev, Mikhail Baloban, Vladislav V. V. Verkhusha, Daria M. M. Shcherbakova
Summary: Researchers developed a non-opsin optogenetic tool for light-controlled nuclear export of a protein in neurons. They found that commonly used nuclear localization signals (NLSs) are suboptimal in neurons and identified uncommon NLSs that mediate fast nuclear import. By adjusting the NLSs, they optimized the tool for lower background and higher contrast in neurons.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Lihong He, Wei Shao, Jiang Li, Fei Deng, Hualin Wang, Zhihong Hu, Manli Wang
Summary: This study systematically analyzed the nuclear localization of 154 ORFs encoded by AcMNPV, with 25 viral proteins found to be localized in the nucleus in the absence of virus infection. Upon virus infection, 11 proteins originally located in the cytoplasm were completely translocated into the nucleus, suggesting their nuclear import is facilitated by other viral or host proteins. Further experiments identified specific proteins that were imported into the nucleus with the assistance of other nuclear localization proteins.
JOURNAL OF GENERAL VIROLOGY
(2021)
Article
Virology
Margarita A. Kurnaeva, Arthur O. Zalevsky, Eugene A. Arifulin, Olga M. Lisitsyna, Anna Tvorogova, Maria Y. Shubina, Gleb P. Bourenkov, Maria A. Tikhomirova, Daria M. Potashnikova, Anastasia Kachalova, Yana R. Musinova, Andrey Golovin, Yegor S. Vassetzky, Eugene Sheval
Summary: This study investigated the integration of nuclear localization signal (NLS) and nucleolar localization signal (NoLS) into the basic domain of HIV-1 Tat and found that these two supplementary functions are embedded within the amino acid sequence. Integration of NLSs and NoLSs into functional domains of viral proteins enriched with positively charged amino acids allows the concentration of different functions within small protein regions and may have influenced viral evolution by preventing an increase in protein size.
JOURNAL OF VIROLOGY
(2022)
Article
Biochemistry & Molecular Biology
Sakura Niinuma, Yui Wake, Yuki Nakagawa, Takehito Kaneko
Summary: By evaluating the localization of Cas9 protein with or without nuclear localization signal (NLS) in mouse embryos, we found that the effective transportation of Cas9 from the cytoplasm to the pronuclei through NLS resulted in increased genome-editing rates in offspring.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2023)
Article
Chemistry, Physical
Gankun Yuan, Qilu Wang, Zifan You, Xuening Chen, Jinping Xue, Xiao Jia, Juanjuan Chen
Summary: The study introduces a targeted PDT strategy using folate-modified poly-beta-cyclodextrin as a carrier, demonstrating high biological safety and efficacy in nuclear-targeted treatment.
Article
Cell Biology
Lin Song, Lijuan Cao, Rui Liu, Hui Ma, Yanan Li, Qianwen Shang, Zhiyuan Zheng, Liying Zhang, Wen Zhang, Yuyi Han, Xiaoren Zhang, Huilin Yang, Ying Wang, Gerry Melino, Changshun Shao, Yufang Shi
Summary: Despite the widespread clinical use of glucocorticoids (GC), their detailed molecular mechanism in inducing osteoporosis is not fully understood. Research shows that lymphocytes play a critical role in regulating GC-induced osteoporosis.
CELL DEATH & DISEASE
(2021)
Article
Cell & Tissue Engineering
Shengchao Zhang, Jiankai Fang, Zhanhong Liu, Pengbo Hou, Lijuan Cao, Yuyan Zhang, Rui Liu, Yanan Li, Qianwen Shang, Yongjing Chen, Chao Feng, Guan Wang, Gerry Melino, Ying Wang, Changshun Shao, Yufang Shi
Summary: This study demonstrates that human muscle stem cells treated with inflammatory factors can alleviate symptoms of inflammatory bowel disease through IDO-mediated TSG-6 production. Mechanistically, tryptophan metabolites produced by IDO enhance TSG-6 expression via activation of the common receptor AHR.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Oncology
Yuxing Wang, Xiaohua Pan, Yanjun Li, Ru Wang, Yuanyuan Yang, Baichun Jiang, Gongping Sun, Changshun Shao, Molin Wang, Yaoqin Gong
Summary: CUL4B promotes TAM resistance in breast cancer cells by upregulating ER-alpha 36 expression through the regulation of miR-32-5p. This mechanism involves epigenetic modifications mediated by CRL4B, PRC2, and HDAC complexes, and targeting these complexes can increase sensitivity to TAM in breast cancer cells.
JOURNAL OF PATHOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Xiaolei Li, Xiao Su, Rui Liu, Yongsha Pan, Jiankai Fang, Lijuan Cao, Chao Feng, Qianwen Shang, Yongjing Chen, Changshun Shao, Yufang Shi
Summary: Pharmacological modulation of macrophage phenotype by HDAC inhibitors can reshape the tumor immune microenvironment, enhance anti-tumor immune responses, and improve the efficacy of immunotherapies in various tumors.
Article
Biochemistry & Molecular Biology
Xiaohe Hao, Wenqing Bu, Guosheng Lv, Limei Xu, Dong Hou, Jing Wang, Xiaojie Liu, Tingting Yang, Xiyu Zhang, Qiao Liu, Yaoqin Gong, Changshun Shao
Summary: The accumulation of excessive mitochondrial superoxide during M-phase arrest induced by anticancer drugs can lead to oxidative DNA damage, compromising cell survival and proliferation. Mitochondrial dysfunction and oxidative stress play a critical role in the antineoplastic effect of M-phase arrest-inducing chemotherapeutics, highlighting the interdependence of cell cycle progression and mitochondrial homeostasis in cancer therapy.
Review
Biochemistry & Molecular Biology
Qing Li, Ying Wang, Qiang Sun, Jasmin Knopf, Martin Herrmann, Liangyu Lin, Jingting Jiang, Changshun Shao, Peishan Li, Xiaozhou He, Fei Hua, Zubiao Niu, Chaobing Ma, Yichao Zhu, Giuseppe Ippolito, Mauro Piacentini, Jerome Estaquier, Sonia Melino, Felix Daniel Weiss, Emanuele Andreano, Eicke Latz, Joachim L. Schultze, Rino Rappuoli, Alberto Mantovani, Tak Wah Mak, Gerry Melino, Yufang Shi
Summary: This article reviews the unprecedented impact of COVID-19 on our daily lives and quality of life over a period of more than two years, focusing on the changes in immune response, virus mutations, vaccines, and treatment strategies. The authors predict that with the use of existing and emerging technologies, we will be able to control the COVID-19 pandemic.
CELL DEATH AND DIFFERENTIATION
(2022)
Article
Cell Biology
Junchao Qin, Tao Huang, Jing Wang, Limei Xu, Qianli Dang, Xiuhua Xu, Hongbin Liu, Zhaojian Liu, Changshun Shao, Xiyu Zhang
Summary: This study reveals an essential role of Rad51 in the maintenance of spermatogonia and meiotic progression in mice.
CELL DEATH DISCOVERY
(2022)
Article
Multidisciplinary Sciences
Zixiang Wang, Shourong Wang, Junchao Qin, Xiyu Zhang, Gang Lu, Hongbin Liu, Haiyang Guo, Ligang Wu, Victoria O. Shender, Changshun Shao, Beihua Kong, Zhaojian Liu
Summary: This study reveals that altered expression of splicing factor BUD31 can enhance ovarian cancer progression by promoting exon inclusion in the anti-apoptotic BCL2 family member, BCL2L12. The findings highlight the critical role of BUD31-regulated exon inclusion in ovarian cancer cell survival and cancer progression.
NATURE COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
Lili Sun, Chen Yao, Xiaojiao Li, Yuxing Wang, Ru Wang, Molin Wang, Qiao Liu, Denise J. Montell, Changshun Shao, Yaoqin Gong, Gongping Sun
Summary: This study reveals that cells can survive executioner caspase activation through a process called anastasis, and these surviving cells have enhanced migration and pro-angiogenic capacity. The activation of p38 MAPK plays a crucial role in anastasis and is essential for the elevated migratory and angiogenic capacity in these cells.
CELL DEATH AND DIFFERENTIATION
(2023)
Review
Biochemistry & Molecular Biology
Yanan Li, Sara Giovannini, Tingting Wang, Jiankai Fang, Peishan Li, Changshun Shao, Ying Wang, Yufang TOR Ctr, Yufang Shi, Eleonora Candi, Gerry Melino, Francesca Bernassola
Summary: Epithelial tissue homeostasis is closely associated with the self-renewal and differentiation behaviors of epithelial stem cells (ESCs). p63, a well-known marker of ESCs, regulates the transcription of genes involved in cell survival, stem cell self-renewal, migration, differentiation, and epithelial-to-mesenchymal transition. p63 plays a crucial role in normal epithelial development and epithelium-derived cancer pathogenesis.
Review
Cell Biology
Rui Liu, Manuel Scimeca, Qiang Sun, Gerry Melino, Alessandro Mauriello, Changshun Shao, Yufang TOR Ctr, Yufang Shi, Mauro Piacentini, Giuseppe Tisone, Massimiliano Agostini
Summary: Liver regeneration is a complex process involving inflammation, granulation, and tissue remodeling. Hepatic macrophages play a crucial role in orchestrating liver regeneration by transitioning between tolerant and inflammatory phenotypes. Dysfunctional macrophages can exacerbate liver injury and promote fibrosis and cirrhosis, highlighting the need for macrophage-targeting strategies. Metabolic rewiring may offer a potential therapeutic approach to modulate macrophage function and promote liver inflammation management and regeneration.
CELL DEATH & DISEASE
(2023)
Review
Cell & Tissue Engineering
Mengting Zhu, Lijuan Cao, Sonia Melino, Eleonora Candi, Ying Wang, Changshun Shao, Gerry Melino, Yufang Shi, Xiaodong Chen
Summary: This review outlines the role of mesenchymal stem/stromal cells (MSCs) in wound healing and regeneration, with a focus on the central role of neutrophils, macrophages, and T cells in the interaction between MSCs and the immune system. The specific effects of MSC-based therapy on different pathological wound healing are also discussed, along with the translation of scientific advances in MSCs into clinical strategies.
STEM CELLS TRANSLATIONAL MEDICINE
(2023)
Review
Immunology
Peishan Li, Qianmin Ou, Songtao Shi, Changshun Shao
Summary: Mesenchymal stem/stromal cells (MSCs) are widely distributed in the body and can be used for the treatment of autoimmune diseases and other chronic disorders. Dental stem cells (DSCs) have remarkable immunomodulatory properties and have therapeutic effects on systemic inflammatory diseases. MSCs derived from nondental tissues, such as the umbilical cord, also show benefits in the management of periodontitis in preclinical studies.
CELLULAR & MOLECULAR IMMUNOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Yuyi Han, Jianxin Yang, Jiankai Fang, Yipeng Zhou, Eleonora Candi, Jihong Wang, Dong Hua, Changshun Shao, Yufang Shi
Summary: Mesenchymal stromal/stem cells (MSCs) possess the potential of multi-lineage differentiation and self-renewal, and their therapeutic effects in treating inflammatory diseases are mainly achieved through the secretion of factors that regulate immune response, tissue remodeling, and cellular homeostasis.
SIGNAL TRANSDUCTION AND TARGETED THERAPY
(2022)
Article
Oncology
Haixia Shan, Bo Wang, Xiaodong Zhang, Hui Song, Xi Li, Yongxin Zou, Baichun Jiang, Huili Hu, Hao Dou, Changshun Shao, Lifen Gao, Chunhong Ma, Xiaoyun Yang, Xiaohong Liang, Yaoqin Gong
Summary: The study found that CUL4B enhances HBV replication by interacting with HBx and disrupting its ubiquitin-dependent proteasomal degradation. The results of this study are of great significance for the development of potential targets for anti-HBV therapy.
CANCER BIOLOGY & MEDICINE
(2022)