Article
Multidisciplinary Sciences
Emilie Montembault, Irene Deduyer, Marie-Charlotte Claverie, Lou Bouit, Nicolas J. J. Tourasse, Denis Dupuy, Derek McCusker, Anne Royou
Summary: Cytokinesis, the process of cellular division, relies on the formation of a contractile ring to partition cellular content. Rho1 GTPase and its RhoGEF (Pbl) play essential roles in this process, but the regulation of Rho1 to maintain correct furrow position remains unclear. In this study, we found that two isoforms of Pbl, with distinct localisation, control Rho1 during asymmetric division in Drosophila neuroblasts. Pbl-A, enriched at the spindlemidzone and furrow, focuses Rho1 to sustain efficient ingression, while Pbl-B, localized on the entire cortex, promotes the broadening of Rho1 activity. This enlarged zone of Rho1 activity is critical for adjusting furrow position and preserving correct daughter cell size asymmetry. Our findings highlight the importance of using isoforms with distinct localisation to enhance the robustness of essential processes.
NATURE COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Hannes Schihada, Thomas M. Klompstra, Laura J. Humphrys, Igor Cervenka, Shamim Dadvar, Peter Kolb, Jorge L. Ruas, Gunnar Schulte
Summary: The G protein-coupled receptor GPR35 is involved in oncogenic signaling, bacterial infections, and inflammatory bowel disease within the intestine. This study reveals that GPR35 has two isoforms with distinct promoters and alternative splicing. The longer isoform limits G protein activation but enhances receptor-beta-arrestin interaction. Structural analysis suggests that a disulfide bridge between the N-terminus and extracellular loop 3 is crucial for G protein activation, while an additional cysteine from the extended N-terminus of the longer isoform limits agonist-induced receptor-beta-arrestin2 interaction. The findings provide insights for developing isoform-specific GPR35 ligands for mechanism-based therapies.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Dristi Bhandari, Ahmed Elshaarrawi, Karen S. Katula
Summary: WNT5A isoforms WNT5A-L(A) and WNT5A-S(B) exhibit distinct and overlapping functions in normal osteoblasts, affecting the expression of differentiation markers and proliferation differently. WNT5A-S(B) enhances beta-catenin signaling, while WNT5A-L(A) increases the proliferation of undifferentiated cells.
JOURNAL OF CELLULAR BIOCHEMISTRY
(2021)
Article
Multidisciplinary Sciences
Omar Arias-Gaguancela, Emily Herrell, Mina Aziz, Kent D. Chapman
Summary: This study compared two FAAH isoforms in the legume Medicago truncatula and found that they exhibited differences in their amidohydrolase activity profiles due to differences in the structural and physicochemical properties of the substrate binding pockets. This first report of the enzymatic behavior of two phylogenetically distinct plant FAAHs will provide a foundation for further investigations regarding FAAH isoforms in legumes and other plant species.
SCIENTIFIC REPORTS
(2023)
Article
Cell Biology
Annika Wylie, Amanda E. Jones, Simanti Das, Wan-Jin Lu, John M. Abrams
Summary: This study reveals opposing features of p53-mediated transcriptional control, with transcriptional repression being achieved through DNA binding sites and correlated with H3K9me3 chromatin marks, independent of stress or Chk2. Targeted knockout of different p53 isoforms dissociated their opposing transcriptional activities, highlighting their important roles in repression and proper germ cell formation.
DEVELOPMENTAL CELL
(2022)
Article
Cell Biology
Amadeus Xu, Angika Basant, Sibylle Schleich, Timothy P. Newsome, Michael Way
Summary: The study reveals that intracellular mature viruses (IMVs) and intracellular enveloped viruses (IEVs) can undergo microtubule-based motility and recruit the kinesin-1 motor protein for transport. IMVs are the first and most abundant infectious form of vaccinia virus during its replication cycle. These findings provide insights into the transport mechanism of the virus.
JOURNAL OF CELL SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Loretta Ferrera, Raffaella Barbieri, Cristiana Picco, Paolo Zuccolini, Alessia Remigante, Sara Bertelli, Maria Rita Fumagalli, Giovanni Zifarelli, Caterina A. M. La Porta, Paola Gavazzo, Michael Pusch
Summary: The response to oxidative stimulation in melanoma cells involves the activation of potassium currents and an increase in intracellular calcium levels, mediated by BK, KCa3.1, and TRPM2 channels. Differences in the expression levels of these channels in different cell lines may play a crucial role in melanoma progression. Targeting these ion channels pharmacologically could be a novel strategy to reduce the metastatic potential of melanoma cells alongside traditional treatments.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Bing-Rui Zhou, Hanqiao Feng, Seyit Kale, Tara Fox, Htet Khant, Natalia de Val, Rodolfo Ghirlando, Anna R. Panchenko, Yawen Bai
Summary: This study used cryo-EM to analyze the structures of chromatosomes containing different H1 isoforms, revealing that the C-terminal tails of the H1 isoforms mainly control the orientations of flanking DNA. The results provide insights into the structures and dynamics of chromatosomes, with implications for the structure and function of chromatin.
Article
Biochemistry & Molecular Biology
Mathieu Pottier, Van Anh Le Thi, Catherine Primard-Brisset, Jessica Marion, Michele Wolf Bianchi, Cindy Victor, Annabelle Dejardin, Gilles Pilate, Sebastien Thomine
Summary: The study finds that there are two copies of NRAMP3 metal transporters in the poplar genome, one of which retains the original function of releasing metals stored in vacuoles in Arabidopsis, while the other copy has acquired a new function in controlling the cell-to-cell transport of manganese in the Trans-Golgi Network. This research provides a clear case of neofunctionalization through a change in the subcellular localization of a metal transporter and evidence for the involvement of the secretory pathway in the cell-to-cell transport of manganese.
MOLECULAR BIOLOGY AND EVOLUTION
(2022)
Article
Biochemistry & Molecular Biology
Matthes Zessin, Marat Meleshin, Lucas Praetorius, Wolfgang Sippl, Cyril Barinka, Mike Schutkowski
Summary: This study systematically analyzed the acyl-specificity of human zinc-dependent HDAC and sirtuin isoforms. The results showed that HDAC3 has a much higher delactoylase activity compared to SIRT2, which was previously believed to be the major in vivo delactoylase. Additionally, HDAC6 and HDAC8 were found to have high depyruvoylase activity.
ACS CHEMICAL BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Yu-Han Lin, Meng-Yun Xu, Chuan-Chih Hsu, Florensia Ariani Damei, Hui-Chun Lee, Wei-Lun Tsai, Cuong V. Hoang, Yin-Ru Chiang, Lay-Sun Ma
Summary: This study reveals the role of fungal PR-1-like proteins in countering plant defense, and how Ustilago maydis utilizes plant proteases to promote fungal virulence.
NATURE COMMUNICATIONS
(2023)
Article
Chemistry, Physical
Christian Aponte-Rivera, Roseanna N. Zia
Summary: Two-point microrheology (TPM) is used to infer material properties of complex fluids from the correlated motion of hydrodynamically interacting probes embedded in the medium. However, previous TPM theory breaks down in confined media, and a new theory is required to account for confinement effects.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2022)
Article
Cell Biology
Ramoji Kosuru, Bandana Singh, Sribalaji Lakshmikanthan, Yoshinori Nishijima, Jeannette Vasquez-Vivar, David X. Zhang, Magdalena Chrzanowska
Summary: Rap1 plays a crucial role in maintaining endothelial cell homeostasis by promoting NO release, with Rap1A and Rap1B exerting distinct effects on endothelial function. Exogenous L-Arg administration can ameliorate endothelial dysfunction resulting from the deletion of both Rap1A and Rap1B isoforms. While Rap1B directly positively regulates eNOS activation, Rap1A prevents negative regulation of eNOS.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Angela L. Nicholson-Shaw, Eric R. Kofman, Gene W. Yeo, Amy E. Pasquinelli
Summary: This study analyzed the RNAs bound by endogenous PABPN or PABPC in human cells, revealing the different preferences of various transcripts for these proteins and shedding light on their roles in poly(A)-tail synthesis, maintenance, and function.
NUCLEIC ACIDS RESEARCH
(2022)
Article
Plant Sciences
Joaquin Royo, Luis M. Muniz, Elisa Gomez, Ana M. Anazco-Guenkova, Gregorio Hueros
Summary: ZmTCRR1 and 2 play a role in the maize endosperm transfer cells. They modulate auxin and cytokinin signaling to affect the differentiation and growth of inner endosperm cells.
Article
Biochemistry & Molecular Biology
Yoichiro Harada, Takehiro Suzuki, Tomoko Fukushige, Yasuhiko Kizuka, Hirokazu Yagi, Mika Yamamoto, Kiyotaka Kondo, Hiromasa Inoue, Koichi Kato, Naoyuki Taniguchi, Takuro Kanekura, Naoshi Dohmae, Ikuro Maruyama
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2019)
Article
Biochemistry & Molecular Biology
Misuzu Hashimoto, Tetsuya Hirata, Chizuko Yonekawa, Kaho Takeichi, Akiyoshi Fukamizu, Tsutomu Nakagawa, Yasuhiko Kizuka
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2020)
Review
Biochemistry & Molecular Biology
Masamichi Nagae, Yoshiki Yamaguchi, Naoyuki Taniguchi, Yasuhiko Kizuka
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Article
Biochemistry & Molecular Biology
Seita Tomida, Misaki Takata, Tetsuya Hirata, Masamichi Nagae, Miyako Nakano, Yasuhiko Kizuka
JOURNAL OF BIOLOGICAL CHEMISTRY
(2020)
Article
Biochemistry & Molecular Biology
Chenyu Ma, Hideyuki Takeuchi, Huilin Hao, Chizuko Yonekawa, Kazuki Nakajima, Masamichi Nagae, Tetsuya Okajima, Robert S. Haltiwanger, Yasuhiko Kizuka
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Review
Biochemistry & Molecular Biology
Naoyuki Taniguchi, Yuki Ohkawa, Kento Maeda, Yoichiro Harada, Masamichi Nagae, Yasuhiko Kizuka, Hideyuki Ihara, Yoshitaka Ikeda
Summary: Cancer-related changes in glycans on cell surface proteins are regulated by glycosyltransferases and related genes, which play key roles in cancer metastasis and invasion. The significance of EMT and N-glycan branching glycosyltransferases needs to be reconsidered for potential therapeutic strategies. Specific glycosyltransferases involved in EMT or MET pathways may be difficult to define due to their roles in intermediate states.
MOLECULAR ASPECTS OF MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Tetsuya Hirata, Masamichi Nagae, Reina F. Osuka, Sushil K. Mishra, Mayumi Yamada, Yasuhiko Kizuka
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2020)
Review
Biochemistry & Molecular Biology
Misuzu Hashimoto, Akiyoshi Fukamizu, Tsutomu Nakagawa, Yasuhiko Kizuka
Summary: PRMT1 is crucial for the development of neurons, astrocytes, and oligodendrocytes, requiring further investigation. The relevance of PRMT1 in neurodegenerative diseases will continue to be a hot topic.
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2021)
Article
Cell Biology
Yi-Fan Huang, Kazuhiro Aoki, Sachiko Akase, Mayumi Ishihara, Yi-Shi Liu, Ganglong Yang, Yasuhiko Kizuka, Shuji Mizumoto, Michael Tiemeyer, Xiao-Dong Gao, Kiyoko F. Aoki-Kinoshita, Morihisa Fujita
Summary: The researchers developed a tool called GlycoMaple to estimate and visualize glycan structures based on gene expression, accelerating glycan analysis and engineering. Through comprehensive glycosylation mapping, gene knockout, and glycomic analysis, the study predicted and compared glycan structures in different cells and tissues.
DEVELOPMENTAL CELL
(2021)
Article
Biochemistry & Molecular Biology
Tetsuya Hirata, Atsushi Kobayashi, Tamio Furuse, Ikuko Yamada, Masaru Tamura, Hiroyuki Tomita, Yuko Tokoro, Akinori Ninomiya, Yoshitaka Fujihara, Masahito Ikawa, Yusuke Maeda, Yoshiko Murakami, Yasuhiko Kizuka, Taroh Kinoshita
Summary: The GPI-GalNAc side chain is essential for the functions of GPI-APs in mammals, particularly in bone and the brain. Knocking out the initiation enzyme PGAP4 in mice leads to various phenotypes and increased vulnerability to prion diseases, highlighting the protective roles of the GalNAc side chain.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Reina F. Osuka, Tetsuya Hirata, Masamichi Nagae, Miyako Nakano, Hiroyuki Shibata, Ryo Okamoto, Yasuhiko Kizuka
Summary: The N-terminal domain of N-acetylglucosaminyltransferase-V (GnT-V) is crucial for efficient glycosylation and plays a key role in the recognition of glycoprotein substrates. This study revealed that the N domain interacts directly with the substrate polypeptide, providing new insights into the mechanism of substrate-selective biosynthesis of N-glycans.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Biology
Tetsuya Hirata, Misaki Takata, Yuko Tokoro, Miyako Nakano, Yasuhiko Kizuka
Summary: The activity of GnT-V in cells is regulated by changing the maturity of cellular N-glycans. Altering the glycosylation state affects the secretion and protein level of GnT-V, which in turn impacts its promotion of cancer growth and malignancy.
COMMUNICATIONS BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Reina F. Osuka, Masamichi Nagae, Akito Ohuchi, Shiho Ohno, Yoshiki Yamaguchi, Yasuhiko Kizuka
Summary: In this study, it was found through molecular dynamics simulations and biochemical experiments that residues outside the catalytic pocket of GnT-V are likely involved in the recognition of the core part of N-glycan. Additionally, the simulation suggested that UDP binding affects the orientation of the acceptor due to conformational changes. These findings provide new insights into how GnT-V recognizes its glycoprotein substrates.
Article
Biochemistry & Molecular Biology
Haruka Kawade, Jyoji Morise, Sushil K. Mishra, Shuta Tsujioka, Shogo Oka, Yasuhiko Kizuka
Summary: This study revealed that the biosynthesis of HNK-1 in the brain and the kidney are differentially regulated by bisecting GlcNAc, with the former being inhibited by the branching sugar. Mechanistically, bisecting GlcNAc suppresses the activity of GlcAT-P but not GlcAT-S, leading to tissue-specific regulation of HNK-1 synthesis.
Article
Biochemistry & Molecular Biology
Masato Kitano, Yasuhiko Kizuka, Tomoaki Sobajima, Miyako Nakano, Kazuki Nakajima, Ryo Misaki, Saki Itoyama, Yoichiro Harada, Akihiro Harada, Eiji Miyoshi, Naoyuki Taniguchi
Summary: Glycosylation is a common posttranslational modification of proteins that is tightly regulated by the subcellular location and transportation of glycosyltransferases. Knockdown of Rab11 results in enhanced sialylation of N-glycans, specifically through the altered localization of the alpha 2,3-sialyltransferase ST3GAL4. This indicates a novel mechanism of glycosyltransferase regulation by Rab11 in post-Golgi compartments.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)