Article
Biology
Jinli Geng, Yingjun Tang, Zhen Yu, Yunming Gao, Wenxiang Li, Yitong Lu, Bo Wang, Huiming Zhou, Ping Li, Nan Liu, Ping Wang, Yubo Fan, Yaxiong Yang, Zengcai Guo, Xiaodong Liu
Summary: GCaMP-X performs well in chronic calcium imaging, causing less damage to neurons compared to traditional GCaMP. Its expression allows researchers to observe spontaneous and global calcium oscillations, with less impact on neural development and neuronal health.
Article
Biochemistry & Molecular Biology
Michal Marcinkowski, Tomas Pilzys, Damian Garbicz, Jan Piwowarski, Kaja Przygonska, Maria Winiewska-Szajewska, Karolina Ferenc, Oleksandr Skorobogatov, Jaroslaw Poznanski, Elzbieta Grzesiuk
Summary: FTO is an N-6-methyladenosine demethylase which can remove methyl groups from nucleic acids, forming complexes with other proteins. Research indicates an interaction between FTO and calmodulin (CaM), influencing calcium-binding loops in CaM.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cardiac & Cardiovascular Systems
Jinhong Wei, Wenting Guo, Ruiwu Wang, John Paul Estillore, Darrell Belke, Yong-Xiang Chen, Alexander Vallmitjana, Raul Benitez, Leif Hove-Madsen, S. R. Wayne Chen
Summary: The physiological significance of PKA phosphorylation of RyR2 in the heart is still poorly understood. Recent structural studies have shown that the PKA phosphorylation site S2030 in RyR2 is located within a pathway that is important for the termination of Ca2+ release. We investigated the impact of S2030 mutations on Ca2+ release termination in cells and generated a mouse model to study the role of S2030 in a physiological setting.
CIRCULATION RESEARCH
(2023)
Review
Biochemistry & Molecular Biology
Anastasiia Bohush, Wieslawa Lesniak, Serge Weis, Anna Filipek
Summary: Parkinson's disease is a neurodegenerative disorder characterized by the formation of Lewy bodies and loss of dopaminergic neurons. Changes in intracellular Ca2+ levels can have serious consequences on cells, with calmodulin playing a crucial role in regulating neuronal plasticity. Therefore, alterations in the activity of calmodulin and its binding proteins may be linked to the development and progression of PD.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell Biology
Samar Sayedyahossein, Louise Thines, David B. Sacks
Summary: The Hippo signaling pathway regulates various cellular processes, including cell proliferation, differentiation, migration, and mechanosensation. Calcium ion (Ca2+) has been found to influence core components of the Hippo pathway. However, the crosstalk between Ca2+ signaling and the Hippo pathway is not fully understood.
CELLULAR SIGNALLING
(2023)
Article
Biochemistry & Molecular Biology
Du-Hyong Cho, Hyeon-Ju Lee, Jee Young Lee, Jung-Hyun Park, Inho Jo
Summary: The study investigated the molecular mechanisms of FIR irradiation inhibiting breast cancer cell proliferation. Results demonstrated that FIR irradiation inhibited cell proliferation by activating the Ca2+/CaM/Chk2 signaling pathway without causing DNA damage.
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Vini Nagaraj, Roy Kim, Talia Martianou, Shyam Kurian, Ashana Nayak, Mukti Patel, Melitta Schachner, Thomas Theis
Summary: The L1 cell adhesion molecule is involved in neural development and repair by stimulating beneficial cellular functions and improving conditions in neurotrauma and the L1 syndrome. L1 agonistic mimetics trigger L1-dependent cellular signaling and promote neurite outgrowth and neuronal migration.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Shuhang Dai, Elisa Venturini, Saveg Yadav, Xiaoxuan Lin, Dylan Clapp, Martin Steckiewicz, Angela M. Gocher-Demske, Arthur M. Edelman, D. Grahame Hardie
Summary: This study reveals the important role of CaMKK2 in mediating EGFR signaling in cancer cells, with regulation mechanisms involving both calcium-dependent and calcium-independent pathways. Additionally, CaMKK2 regulates transcription of key genes, impacting cell survival and proliferation.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Ling Li, Li Li, Ying Shao, Runyu Du, Ling Li, Xiaoguang Shi, Yu Bai
Summary: Insulin resistance (IR) plays a significant role in glucose metabolic disturbance and placental dysplasia in gestational diabetes mellitus (GDM). Calcium/calmodulin dependent protein kinase IV (CAMK4) has been shown to improve insulin IR induced by a high-fat diet (HFD). This study aimed to investigate the role and potential mechanism of CAMK4 in GDM.
MOLECULAR MEDICINE
(2023)
Article
Biochemistry & Molecular Biology
Fu-Sheng Liu, Chang Jiang, Zheng Li, Xiao-Bin Wang, Jing Li, Bing Wang, Guo-Hua Lv, Fu-Bing Liu
Summary: This study establishes an in vitro model to explore the relationship among spinal cord injury, intracellular calcium ions, and autophagy. Mechanical injury is found to promote the increase of intracellular calcium concentration, which activates autophagy through the CaMKK beta/AMPK/mTOR pathway and also triggers apoptosis. This study provides new insights into the mechanisms of spinal cord injury and opens up new possibilities for its treatment.
NEUROCHEMICAL RESEARCH
(2023)
Article
Plant Sciences
Kyle Symonds, Howard Teresinski, Bryan Hau, David Chiasson, Kirsten Benidickson, William Plaxton, Wayne A. Snedden
Summary: Calmodulin-like proteins (CMLs) are the largest family of calcium-binding proteins in plants. The study found that CML13 and CML14 play important roles in plant development, regulating development and morphological features through interactions with other proteins. Inhibition of CML13 or CML14 transcript levels leads to abnormal plant development, including difficulties in germination, chlorosis, and leaf senescence.
PLANT AND CELL PHYSIOLOGY
(2023)
Article
Multidisciplinary Sciences
Giovanna Nalesso, Anne-Sophie Thorup, Suzanne Elizabeth Eldridge, Anna De Palma, Amanpreet Kaur, Kiran Peddireddi, Kevin Blighe, Sharmila Rana, Bryony Stott, Tonia Louise Vincent, Bethan Lynne Thomas, Jessica Bertrand, Joanna Sherwood, Antonella Fioravanti, Costantino Pitzalis, Francesco Dell'Accio
Summary: The WNT/CaMKII signaling pathway plays a crucial role in osteoarthritis by affecting the expression of markers such as HMOX1 and Sox-9. Inhibition of CaMKII may exacerbate cartilage damage and bone remodeling, while also upregulating the expression of matrix remodeling enzymes in articular chondrocytes. These findings suggest the potential therapeutic implications of targeting the WNT/CaMKII pathway in osteoarthritis.
SCIENTIFIC REPORTS
(2021)
Review
Biochemistry & Molecular Biology
Stephen A. Chetwynd, Simon Andrews, Sarah Inglesfield, Christine Delon, Nicholas T. Ktistakis, Heidi C. E. Welch
Summary: Norbin is a highly conserved adaptor protein that plays important roles in neuronal morphology, synaptic plasticity, brain development, and immune response. It regulates cell responses by binding to its interactors, including G protein-coupled receptors (GPCRs), and controls GPCR trafficking and signaling. Dysregulation of Norbin is associated with various neurological conditions. This review summarizes the current understanding of Norbin's structure, expression, and roles in health and disease, with a focus on GPCR trafficking and signaling.
BIOCHEMICAL SOCIETY TRANSACTIONS
(2023)
Article
Biochemistry & Molecular Biology
Ikuko Kakizaki, Takayasu Kobayashi, Shinri Tamura, Hiroshi Akagi, Keiichi Takagaki
Summary: This study found that chondroitin sulfate E and heparin can upregulate neural specific markers during neural differentiation, with chondroitin sulfate E shown to play a role in spheroid formation, while a hyaluronan oligosaccharide mixture downregulated the expressions of myelin specific markers.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Multidisciplinary Sciences
Aurelia C. Balestra, Konstantinos Koussis, Natacha Klages, Steven A. Howell, Helen R. Flynn, Marcus Bantscheff, Carla Pasquarello, Abigail J. Perrin, Lorenzo Brusini, Patrizia Arboit, Olalla Sanz, Laura Peces-Barba Castano, Chrislaine Withers-Martinez, Alexandre Hainard, Sonja Ghidelli-Disse, Ambrosius P. Snijders, David A. Baker, Michael J. Blackman, Mathieu Brochet
Summary: The multipass membrane protein ICM1 is tightly associated with PKG and plays a crucial role in calcium signaling in the malaria parasite. Depletion of ICM1 leads to blocked intracellular calcium mobilization and impacts the parasite's lifecycle transitions and transmission.
Article
Immunology
Xiu-xiu Liu, Lin Yang, Ling-xiao Shao, Yang He, Gang Wu, Yu-huan Bao, Nan-nan Lu, Dong-mei Gong, Ya-ping Lu, Tian-tian Cui, Ning-he Sun, Dan-yang Chen, Wei-xing Shi, Kohji Fukunaga, Hong-shan Chen, Zhong Chen, Feng Han, Ying-mei Lu
JOURNAL OF EXPERIMENTAL MEDICINE
(2020)
Article
Biotechnology & Applied Microbiology
Nopparat Suthprasertporn, Nopparada Mingchinda, Kohji Fukunaga, Wipawan Thangnipon
Article
Neurosciences
Ryo Inagaki, Shigeki Moriguchi, Kohji Fukunaga
MOLECULAR NEUROBIOLOGY
(2020)
Article
Neurosciences
Hisanao Izumi, Keita Sato, Kazuhiro Kojima, Takashi Saito, Takaomi C. Saido, Kohji Fukunaga
Review
Biochemistry & Molecular Biology
Ichiro Kawahata, Kohji Fukunaga
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Article
Cell Biology
Yusuke Isozaki, Kouki Sakai, Kenta Kohiro, Katsuhiko Kagoshima, Yuma Iwamura, Hironori Sato, Daniel Rindner, Sachiko Fujiwara, Kazunari Yamashita, Kensaku Mizuno, Kazumasa Ohashi
MOLECULAR BIOLOGY OF THE CELL
(2020)
Article
Neurosciences
Kazuya Matsuo, Yasushi Yabuki, Kohji Fukunaga
Article
Behavioral Sciences
Hidaka Haga, Ryo Yamada, Hisanao Izumi, Yasuharu Shinoda, Ichiro Kawahata, Hiroyuki Miyachi, Kohji Fukunaga
PHARMACOLOGY BIOCHEMISTRY AND BEHAVIOR
(2020)
Article
Engineering, Biomedical
Luqman Khan, Katsumi Sato, Shinichi Okuyama, Takeshi Kobayashi, Kazumasa Ohashi, Katsuya Hirasaka, Takeshi Nikawa, Kunio Takada, Atsushi Higashitani, Kenji Abiko
JOURNAL OF THE MECHANICAL BEHAVIOR OF BIOMEDICAL MATERIALS
(2020)
Article
Biochemistry & Molecular Biology
Dian Yuan, An Cheng, Ichiro Kawahata, Hisanao Izumi, Jing Xu, Kohji Fukunaga
Summary: SAK3, like donepezil, shows significant effects in improving cognitive and emotional behaviors, as well as reducing oxidative stress levels in OBX mice. The antioxidative effects of SAK3 are similar to those of donepezil, suggesting they are among the neuroprotective mechanisms in AD pathogenesis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell Biology
Komaki Ninomiya, Kai Ohta, Kazunari Yamashita, Kensaku Mizuno, Kazumasa Ohashi
Summary: PLEKHG4B plays a crucial role in epithelial cell-cell junction formation by regulating actin remodeling and modulating the activities of Cdc42 and RhoA. Knockdown of PLEKHG4B leads to open junctions and aberrant myosin activity.
JOURNAL OF CELL SCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Yifei Wang, Yasuharu Shinoda, An Cheng, Ichiro Kawahata, Kohji Fukunaga
Summary: The research revealed that the interaction between FABP5 and α Syn can lead to mitochondrial dysfunction in neuronal cells, increasing the risk of PD development.
Article
Biochemistry & Molecular Biology
Qingyun Guo, Ichiro Kawahata, Tomohide Degawa, Yuri Ikeda-Matsuo, Meiling Sun, Feng Han, Kohji Fukunaga
Summary: The study demonstrates that FABPs may exacerbate infarct volumes after ischemic stroke, and inhibiting FABPs can alleviate ischemic injury. MF6 inhibits FABPs and reduces inflammation-associated prostaglandin E-2 levels, potentially serving as a neuroprotective therapy for ischemic stroke.
Review
Biochemistry & Molecular Biology
Qingyun Guo, Ichiro Kawahata, An Cheng, Wenbin Jia, Haoyang Wang, Kohji Fukunaga
Summary: Stroke is a leading cause of death and disability worldwide, but effective neuroprotective therapies for ischemic stroke patients are lacking. Lipids play important roles in the pathogenesis of neurological diseases, and fatty acid-binding proteins (FABPs) have been found to be involved in neurodegenerative diseases. However, FABPs' roles in ischemic stroke have received comparatively little attention. In this review, we summarize the literature on the associations between FABPs and ischemia and propose potential therapeutic targets for ischemic stroke.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Wenbin Jia, Ichiro Kawahata, An Cheng, Takuya Sasaki, Toshikuni Sasaoka, Kohji Fukunaga
Summary: We confirmed that the FABP3 inhibitor, MF1, successfully reduces nicotine-induced conditioned place preference in mice. MF1 treatment decreased CPP scores in a dose-dependent manner by inhibiting the activation of CaMKII and ERK in the nucleus accumbens and hippocampus.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)