Article
Biochemistry & Molecular Biology
Zarley Rebholz, Jason Lancaster, Hailey Larose, Ashot Khrimian, Katrin Luck, Michael E. Sparks, Kerry L. Gendreau, Leena Shewade, Tobias G. Koellner, Donald C. Weber, Dawn E. Gundersen-Rindal, Paul O'Maille, Alexandre Morozov, Dorothea Tholl
Summary: Insects use small molecules for communication and defense, but the origin and evolution of enzymes involved in terpene biosynthesis in insect genomes is still poorly understood. We studied the evolution of isoprenyl diphosphate synthase-like genes with terpene synthase function in the stink bug family and found evidence for their emergence over 100 million years ago. Different stink bugs maintain small families of these genes, while TPS genes in plants diversified more. Our findings suggest differences in TPS diversification in insects and plants in conjunction with different modes of gene functionalization in chemical interactions.
INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY
(2023)
Article
Chemistry, Physical
Xiaoguang Yan, Jiahui Zhou, Jianjun Ge, Weiguo Li, Dongmei Liang, Warispreet Singh, Gary Black, Shengxin Nie, Jian Liu, Meiqing Sun, Jianjun Qiao, Meilan Huang
Summary: This study elucidates the catalytically active structure of TPS JeST4 from nonseed plants, revealing the critical residues and intermediates involved in catalysis. Mutations were introduced to improve the catalytic efficiency, leading to the discovery of an unusual product. The computational-guided engineering of nonseed plant TPS provides new insights for the production of valuable terpenoid products.
Article
Plant Sciences
Zerui Yang, Ting Zhan, Chunzhu Xie, Song Huang, Xiasheng Zheng
Summary: In this study, 82 CcTPS genes were identified and characterized in the camphor tree genome. Tandem duplication was observed within the TPS family, and three genes with relatively high expression in the borneol-type camphor tree were found, indicating the presence of multiple BPPSs within a single species. Furthermore, other six CcTPS genes were found to be involved in the biosynthesis of other monoterpenes in camphor tree. Overall, this study provides insights into the role of the CcTPS gene family and the biosynthesis of monoterpenes in camphor tree.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2023)
Article
Agricultural Engineering
Min Lv, Xu Sun, Decong Li, Guo Wei, Lin Liu, Feng Chen, Yongping Cai, Honghong Fan
Summary: This study provides novel insights into the catalytic and regulatory mechanisms underlying the biosynthesis of volatile terpenoids in Dendrobium officinale. Transcriptome analysis and terpene metabolic profiling identified a sesquiterpene synthase gene and several MYB genes involved in the biosynthesis and regulation of terpenoids. The study sheds light on the functional relationships between these genes and their roles in terpenoid biosynthesis.
INDUSTRIAL CROPS AND PRODUCTS
(2022)
Article
Plant Sciences
Wintai Kamolsukyeunyong, Wissarut Sukhaket, Kitsada Pitija, Pornwalai Thorngkham, Sugunya Mahatheeranont, Theerayut Toojinda, Apichart Vanavichit
Summary: The rice sesquiterpene synthase II gene plays a role in the antixenosis defense mechanism against brown planthopper infestation, with differences in functionality observed between resistant and susceptible rice varieties. Single-nucleotide polymorphisms in the gene's promoter region and exon 5, as well as a seven amino acid deletion in the protein sequence, may negatively regulate gene function. Analysis suggests a correlation between SNPs in specific genes and the expression of OsSTPS2, while a 21 bp deletion in exon 5 affects the antixenosis mechanism during BPH infestation.
Article
Plant Sciences
Varun Dwivedi, Sarma Rajeev Kumar, H. B. Shilpashree, Ram Krishna, Srinivas Rao, Ajit K. Shasany, Shannon B. Olsson, Dinesh A. Nagegowda
Summary: Research has found that terpene synthase StTPS18 plays an important role in bacterial defense in potatoes. Overexpression of StTPS18 can enhance tolerance to bacterial pathogens by upregulating the expression of phytosterol biosynthesis and defense genes.
Article
Forestry
Yunxiao Zhao, Yicun Chen, Ming Gao, Liwen Wu, Yangdong Wang
Summary: In this study, the regulatory mechanism of monoterpene production in Litsea cubeba was investigated. LcTPS32 was identified as a monoterpene synthase and was found to increase monoterpene production in L. cubeba by regulating the MVA and MEP pathways. Additionally, LcMYB106 was found to negatively regulate monoterpene biosynthesis by directly binding to the promoter of LcTPS32.
Article
Plant Sciences
B. Markus Lange, Narayanan Srividya, Iris Lange, Amber N. Parrish, Lukas R. Benzenberg, Iovanna Pandelova, Kelly J. Vining, Matthias Wuest
Summary: The essential oils extracted from above-ground materials of mint family members are widely used in consumer products. Previous research focused on the characterization of terpenoid oil constituents in mint leaves, but we found that other organs, such as stems, rhizomes, and roots, also emit volatiles with varying compositions. The analysis of volatile quantities released by mint plants treated with Verticillium dahliae-spore suspension or water showed differences in susceptible and resistant species, indicating their involvement in disease resistance.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Shogo Iwakata, Kazuya Asada, Tomoyuki Nishi, Rafaella Stepanova, So Shinoda, Daijiro Ueda, Masahiro Fujihashi, Yoko Yasuno, Tetsuro Shinada, Tsutomu Sato
Summary: This study reveals the mechanism of geranyl-beta-phellandrene formation by Terpene Synthase from Bacillus alcalophilus and successfully alters the product profiles by targeting specific amino acid residues. This research is of great significance in deepening our understanding of the catalytic mechanism of terpene synthases.
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
(2022)
Review
Biochemistry & Molecular Biology
Li-Min Huang, Hsin Huang, Yu-Chen Chuang, Wen-Huei Chen, Chun-Neng Wang, Hong-Hwa Chen
Summary: Terpenoids are important compounds in plants with functions of attracting pollinators, defense, and insecticidal properties. The evolution of terpene synthases (TPSs) in Orchidaceae, one of the largest and most diverse families of flowering plants, shows a diversification pattern with increased duplicated copies in derived orchid species.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Agriculture, Multidisciplinary
Songyu Liu, Bingqi Shan, Xiaomiao Zhou, Wenping Gao, Yaran Liu, Baoqing Zhu, Lei Sun
Summary: This study investigates the regulation of monoterpenoid biosynthesis in grapes by analyzing the monoterpenoid profiles and transcriptome data of four grape hybrids. Candidate genes and transcription factors related to monoterpenoid biosynthesis are identified, enhancing our understanding of this process.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Zarley Rebholz, Leena Shewade, Kylie Kaler, Hailey Larose, Florian Schubot, Dorothea Tholl, Alexandre V. Morozov, Paul E. O'Maille
Summary: Insects have developed a chemical communication system involving terpenoids, which were previously thought to be produced exclusively by plants and microbes. Through gene discovery and characterization of insect terpene synthases (TPSs), researchers have found that isopentenyl diphosphate synthases (IDS), enzymes from primary isoprenoid metabolism, may have played a role in the evolutionary emergence of TPS function. By identifying mechanistic differences between IDS and TPS enzymes, they proposed that the loss of isopentenyl diphosphate (IPP) binding motifs is linked to the gain of TPS function. This study provides insights into the evolution and functions of insect TPSs.
Article
Biochemistry & Molecular Biology
Pravesh Bhargav, Seema Chaurasia, Aashish Kumar, Gaurav Srivastava, Yatish Pant, Chandan Singh Chanotiya, Sumit Ghosh
Summary: This study identified a suite of terpene synthase (TPS) genes potentially involved in biosynthesis of resin volatiles in Boswellia. BsTPS2, a bark-preferentially expressed and wound-inducible TPS, was biochemically characterized and found to be involved in the synthesis of (S)-( +)-linalool, a monoterpene resin volatile with a known role in plant defense.
PLANT MOLECULAR BIOLOGY
(2023)
Article
Agronomy
Xuemei Fu, Nan Yang, Yongqin Du, Hafiz Muhammad Kamran, Huabo Wang, Shaoyuan Chen, Longqing Chen
Summary: Terpene synthase (TPS) plays a crucial role in the biosynthesis of terpenoids in wintersweet, with a total of 52 CpTPS genes identified in this study. These genes were classified into 5 subfamilies, and 146 SSRs were found. Genetic analysis showed that wintersweet samples can be divided into 2 clusters and 4 groups. This study provides valuable insights for terpenoid biosynthesis, genetic linkage mapping, and breeding of wintersweet.
Article
Genetics & Heredity
Zequn Chen, Xiwu Qi, Xu Yu, Ying Zheng, Zhiqi Liu, Hailing Fang, Li Li, Yang Bai, Chengyuan Liang, Weilin Li
Summary: In this study, the TPS gene family in Mentha longifolia was genome-wide identified and analyzed. A total of 63 TPS genes were identified, which could be divided into six subfamilies. The TPS-b subfamily had the largest number of genes, and the TPS-e subfamily showed significant species-specific expansion in M. longifolia compared to other Lamiaceae plant species.
Article
Biochemistry & Molecular Biology
Ghader Bashiri, Laura V. Nigon, Ehab N. M. Jirgis, Ngoc Anh Thu Ho, Tamsyn Stanborough, Stephanie S. Dawes, Edward N. Baker, Esther M. M. Bulloch, Jodie M. Johnston
JOURNAL OF BIOLOGICAL CHEMISTRY
(2020)
Article
Plant Sciences
Yunliu Zeng, Mindy Y. Wang, Denise C. Hunter, Adam J. Matich, Peter A. McAtee, Mareike Knabel, Cyril Hamiaux, Elizabeth A. Popowski, Sara R. Jaeger, Niels J. Nieuwenhuizen, Yar-Khing Yauk, Ross G. Atkinson
Article
Biochemistry & Molecular Biology
Ghader Bashiri, Edward N. Baker
CURRENT OPINION IN STRUCTURAL BIOLOGY
(2020)
Article
Agriculture, Multidisciplinary
Wu Wang, Jiao Feng, Lingling Wei, Muhammad Khalil-Ur-Rehman, Niels J. Nieuwenhuizen, Lina Yang, Huan Zheng, Jianmin Tao
Summary: Our study investigates the biosynthesis of terpenes in grapes at different phenological stages, revealing distinct concentrations of terpenes and gene expression patterns. Through transcriptomic analysis and terpene profiling, we identified key genes and transcription factors potentially involved in the regulation of terpenoid biosynthesis in grapes. Transient overexpression of specific genes in Nicotiana benthamiana leaves confirmed their role in terpene production.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Plant Sciences
Lara Brian, Ben Warren, Peter McAtee, Jessica Rodrigues, Niels Nieuwenhuizen, Asher Pasha, Karine M. David, Annette Richardson, Nicholas J. Provart, Andrew C. Allan, Erika Varkonyi-Gasic, Robert J. Schaffer
Summary: Transcriptomic analysis of the Actinidia chinensis genome identified 2839 transcription factors (TFs) which were grouped into 14 modules, with certain modules correlating to flower and fruit development. This study provides insight into the complexity of transcriptional control of flower and fruit development, offering a new resource for the plant community.
Article
Agronomy
Ruiling Wang, Simona Nardozza, Niels J. Nieuwenhuizen, Tianchi Wang, Mindy Y. Wang, Helen L. Boldingh, Karine M. David, Ross G. Atkinson, Jeremy N. Burdon, Andrew C. Allan, Erika Varkonyi-Gasic, Robert J. Schaffer
Summary: Previous studies have shown that perennial kiwifruit converted to continuously flowering allows rapid cycling of generations, providing a model system for testing gene function and continuous fruit production. By manipulating growing conditions, the gene-edited plants can produce fruit with controlled development and ripening processes, despite not undergoing winter dormancy.
NEW ZEALAND JOURNAL OF CROP AND HORTICULTURAL SCIENCE
(2021)
Article
Plant Sciences
Xiaobei Wang, Junren Meng, Li Deng, Yan Wang, Hui Liu, Jia-Long Yao, Nicolaas Jacobus Nieuwenhuizen, Zhiqiang Wang, Wenfang Zeng
Summary: Auxin and ethylene are critical for peach fruit ripening, with the ILR gene PpILR1 playing a key role in regulating this process through the interaction between the two phytohormones. PpILR1 acts as a transcriptional activator of PpACS1 and hydrolyzes auxin substrates to release free auxin, influencing ethylene and strigolactone-related phenotypes in transgenic tomato plants. These findings provide insights into the role of IAA-amino acid hydrolases in plants and their impact on fruit cultivation and storage.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Plant Sciences
Niels J. Nieuwenhuizen, Xiuyin Chen, Mickael Pellan, Lei Zhang, Lindy Guo, William A. Laing, Robert J. Schaffer, Ross G. Atkinson, Andrew C. Allan
Summary: In kiwifruit, the transcriptional regulation of ethylene biosynthesis is different between leaves and fruit after mechanical wounding. Ethylene biosynthesis in fruit is accompanied by transcriptional increases in ACS, ACO, and NAC TFs, while in kiwifruit leaves, wound-specific transcriptional increases are largely absent, suggesting post-transcriptional control mechanisms are more important. The NAC TFs AcNAC2-4 regulate both system-1 and -2 ethylene biosynthesis in fruit through control of AcACS1 expression levels, but not in leaves where post-transcriptional/translational regulatory mechanisms may prevail.
Review
Chemistry, Inorganic & Nuclear
Lindsay A. Davis, Mercy A. Oyugi, Jamariya Howard, Juan Corrales, Alaa Aziz, Charlene Mandimutsira, Joisha Girme, Amina Agbonoga, Ghader Bashiri, Edward N. Baker, Kayunta Johnson-Winters
Summary: F420-dependent glucose-6-phosphate dehydrogenase (FGD1) plays a crucial role in various bacteria, and its catalytic mechanism and structural properties have been extensively studied, leading to the recent discovery and investigation of similar enzymes in other microorganisms.
INORGANICA CHIMICA ACTA
(2021)
Article
Biochemistry & Molecular Biology
Xiaobei Wang, Lei Pan, Yan Wang, Junren Meng, Li Deng, Liang Niu, Hui Liu, Yifeng Ding, Jia-Long Yao, Nicolaas Jacobus Nieuwenhuizen, Charles Ampomah-Dwamena, Zhenhua Lu, Guochao Cui, Zhiqiang Wang, Wenfang Zeng
Summary: This study reveals that PpIAA1 and PpERF4 play crucial roles in peach fruit ripening by forming a complex and enhancing the expression of genes related to ethylene biosynthesis and fruit softening.
Article
Plant Sciences
Cancan Zhu, Wu Wang, Yu Chen, Yuqiang Zhao, Shijie Zhang, Fenghou Shi, Muhammad Khalil-Ur-Rehman, Niels J. Nieuwenhuizen
Summary: This study investigates the chemical and transcriptional differences in response to chestnut gall wasp (GWDK) infestation in two different chestnut varieties. The results show that resistant varieties have higher concentrations of H2O2 and activities of POD and SOD enzymes, while susceptible varieties have higher activities of CAT and PPO enzymes. Transcriptomic analyses reveal that various metabolic pathways involved in GWDK stress responses are enriched in the contrasting samples.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Edwige J. F. Souleyre, Niels J. Nieuwenhuizen, Mindy Y. Wang, Robert A. Winz, Adam J. Matich, Nadeesha R. Ileperuma, Haidee Tang, Samantha J. Baldwin, Tianchi Wang, Blake W. List, Kirsten A. Hoeata, Elizabeth A. Popowski, Ross G. Atkinson
Summary: Genetic, sensory, and biochemical analyses highlight the significance of three alcohol acyl transferase genes in the production of volatile esters and flavor intensity in ripe kiwifruit.
Article
Plant Sciences
Xiuyin Chen, Mindy Y. Wang, Cecilia H. Deng, Ron A. Beatson, Kerry R. Templeton, Ross G. Atkinson, Niels J. Nieuwenhuizen
Summary: This study analyzed the volatile terpene compounds in ripe hop cones of 21 hop cultivars grown in New Zealand using gas chromatography-mass spectrometry. Different quantities of beta-myrcene, alpha-humulene, and beta-caryophyllene were found in all cultivars. Other terpenes were present in only a subset of cultivars. A total of 87 putative terpene synthase genes were identified using the published hop genome. Four terpene synthase genes were shown to produce key aroma volatiles in ripe hop cones.
Article
Biochemical Research Methods
Paul G. Young, Jacob M. Paynter, Julia K. Wardega, Martin J. Middleditch, Leo S. Payne, Edward N. Baker, Christopher J. Squire
Summary: Cell-surface proteins called adhesins enable bacteria to inhabit specific environments, and adhesins in Gram-positive bacteria often have covalent intramolecular cross-links formed by autocatalysis. During the investigation of the prevalence of such cross-links, a unique adhesin was discovered in Mobiluncus mulieris, a pathogen associated with bacterial vaginosis. This adhesin belongs to the family of proteins containing thioester, isopeptide, and ester bonds (TIE proteins) and shows a critical role in pathogenicity and/or persistence.
ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Xiaoyao Wang, Yunliu Zeng, Niels J. Nieuwenhuizen, Ross G. Atkinson
Summary: The aroma of fruits plays a crucial role in consumer acceptability, with terpene volatiles such as α-terpinolene and 1,8-cineole contributing to the characteristic aroma of ripe kiwifruit. The emission of these compounds is induced by ethylene and is related to the expression of two monoterpene synthase genes in kiwifruit.
PLANT SIGNALING & BEHAVIOR
(2021)