Article
Microbiology
Ahmet Ozdilek, Jiachen Huang, Rachelle Babb, Amy Paschall, Dustin R. Middleton, Jeremy A. Duke, Liise-Anne Pirofski, Jarrod J. Mousa, Fikri Y. Avci
Summary: Capsular polysaccharides (CPSs) are major virulence factors on the surfaces of many human bacterial pathogens. The predominant use of antibodies expressing VH3 gene family in interactions with CPSs has been observed in humans and mice, with critical amino acids in carbohydrate binding identified through mutational analysis. This study provides insights into the structural requirements for interactions between CPSs and antibodies, with implications for knowledge-based vaccine design.
Article
Chemistry, Organic
Jiaming Ao, Xiaoya Zhao, Siai Zhou, Yajing Guo, Guoqing Wang, Sixian Fang, Xuewen Yao, Yuhua Liu, Akihiro Ishiwata, Katsunori Tanaka, Feiqing Ding, Hui Cai
Summary: The core repeating tetrasaccharide unit of the prevalent Streptococcus pneumoniae 23F capsule polysaccharide was synthetized through a stereocontrolled reaction mediated by a zinc cation. The successful synthesis was achieved by using a specific reaction pathway and the directing effect of the metal ion.
ORGANIC CHEMISTRY FRONTIERS
(2023)
Article
Immunology
Allison N. Dammann, Anna B. Chamby, Francisco J. Gonzalez, Molly E. Sharp, Karina Flores, Ifrah Shahi, Sophia Dongas, Thomas A. Hooven, Adam J. Ratner
Summary: This study found that specific capsule types may provide an advantage in GBS vaginal colonization, but the success of certain GBS lineages may involve both capsule and non-capsule genetic elements.
JOURNAL OF INFECTIOUS DISEASES
(2022)
Article
Microbiology
Robert E. Gertz, Fabiana C. Pimenta, Sopio Chochua, Shanda Larson, Anne-Kathryn Venero, Godfrey Bigogo, Jennifer Milucky, Maria da Gloria Carvalho, Bernard Beall
Summary: This study highlights the presence of nonpneumococcal strains carrying genes similar to those of pneumococcal capsular serotypes, potentially affecting population immunity to circulating pneumococcal strains. The overlapping serotypes between nonpathogenic commensal species and Streptococcus pneumoniae may have implications for pneumococcal epidemiology and vaccine strategies. The discovery of nonpneumococcal strains expressing serotypes identical to those targeted by pneumococcal conjugate vaccines expands our understanding of the distribution and potential impact of these strains.
Article
Chemistry, Applied
Tiantian Sun, Shiyan Mai, Hongzhao Mao, Huiting Li, Yunyao Duan, Shuai Meng, Jiaolin Bao, Ning Ding, Chengli Zong
Summary: Around 100 serotypes of Streptococcus pneumonia (Spn) have been discovered, with 90% of severe diseases in children caused by 13 serotypes. The burden of pneumococcal disease has been significantly reduced with the success of pneumococcal bacterial polysaccharide conjugate vaccines (PCVs). The non-vaccine serotype 31 has increased in prevalence. The revised structure of the serotype 31 polysaccharide (s-31-ps) has been reported, and a conjugate vaccine has been prepared using organic solvent instead of water.
CARBOHYDRATE POLYMERS
(2022)
Article
Chemistry, Applied
Walmik Karbhari Gaikwad, Kisan M. Kodam, Rajeev M. Dhere, Swapan K. Jana, Manish Gautam, Asha D. Mallya, Dipen Soni, Sudhakar Bhagade, Ashishkumar Gulahne
Summary: The study developed a method for purification and depolymerization of Streptococcus pneumoniae serotype 2 CPS, using TFA treatment to achieve both processes simultaneously. Results showed that the antigenicity of CPS was not affected after purification and depolymerization.
CARBOHYDRATE POLYMERS
(2021)
Article
Biochemistry & Molecular Biology
Johanna J. Kenyon, Anastasiya A. Kasimova, Anastasiya N. Sviridova, Anna M. Shpirt, Mikhail M. Shneider, Yuliya Mikhaylova, Andrei A. Shelenkov, Anastasiya Popova, Andrei Perepelov, Alexander S. Shashkov, Andrei S. Dmitrenok, Alexander O. Chizov, Yuriy A. Knirel
Summary: A novel KL144 capsular polysaccharide biosynthesis gene cluster was identified, and its structure was analyzed. Comparison of different gene clusters revealed shared genes and discussed enzymes that may influence the formation of glycosidic linkages in the structures.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Immunology
Javid Aceil, Amy Paschall, Cory J. Knoot, Lloyd S. Robinson, Nichollas E. Scott, Mario F. Feldman, Christian M. Harding, Fikri Y. Avci
Summary: Capsular polysaccharides are widely used as the main components of glycoconjugate vaccines against bacterial diseases. Pneumococcal conjugate vaccines are administered globally to prevent pneumococcal disease. Our previous research has shown the efficacy of a bioconjugation method in producing pneumococcal conjugate vaccines.
Article
Immunology
Elena Mitsi, Daniella McLenaghan, Asia-Sophia Wolf, Scott Jones, Andrea M. Collins, Angela D. Hyder-Wright, David Goldblatt, Robert S. Heyderman, Stephen B. Gordon, Daniela M. Ferreira
Summary: This study found that immunization with 13-valent pneumococcal conjugate vaccine elicits serotype-specific immunoglobulin G (IgG) responses, with serum IgG levels to correlate with those measured in bronchoalveolar lavage samples, but shows poor immunogenicity against serotype 3 in both sites.
JOURNAL OF INFECTIOUS DISEASES
(2022)
Article
Microbiology
Oluwaseun Rume-Abiola Oyewole, Phung Lang, Werner C. Albrich, Kerstin Wissel, Stephen L. Leib, Carlo Casanova, Markus Hilty
Summary: Pneumococcal conjugate vaccines have significantly decreased the incidence of invasive pneumococcal disease worldwide, but the impact of regional vaccine uptake differences on IPD epidemiology remains unclear. A study in Switzerland found that while PCV coverage varied between regions, the overall IPD incidence in the PCV13 era only decreased by 16% compared to the PCV7 era. Increased PCV coverage led to reductions in vaccine-type and overall IPD incidence across all age groups, but a rise in non-vaccine-type IPD was observed in older adults.
Article
Infectious Diseases
Guijuan Hao, Rundong Shu, Liqin Ding, Xia Chen, Yonghao Miao, Jiaqi Wu, Haijian Zhou, Hui Wang
Summary: A new long-tailed phage, phi SRD2021, was discovered to have potential therapeutic effects against K. pneumoniae infections by recognizing the K47 capsular polysaccharide as a receptor for infection. It showed therapeutic efficacy against CRKP infections and significantly reduced colonized CRKP in mouse intestines.
Article
Microbiology
Feroze A. Ganaie, Jamil S. Saad, Stephanie W. Lo, Lesley McGee, Stephen D. Bentley, Andries J. van Tonder, Paulina Hawkins, Jeremy D. Keenan, Juan J. Calix, Moon H. Nahm
Summary: Streptococcus pneumoniae produces diverse capsule types, posing a threat to vaccines targeting pneumococcal polysaccharide capsule. Many capsule types remain uncharacterized. Identifying functional determinants of capsule synthesis is important for improving typing methods and discovering novel variants.
JOURNAL OF CLINICAL MICROBIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Vikrant Minhas, Arnau Domenech, Dimitra Synefiaridou, Daniel Straume, Max Brendel, Gonzalo Cebrero, Xue Liu, Charlotte Costa, Mara Baldry, Jean-Claude Sirard, Camilo Perez, Nicolas Gisch, Sven Hammerschmidt, Leiv Sigve Havarstein, Jan-Willem Veening
Summary: Competence development in Streptococcus pneumoniae controls genetic transformation, biofilm formation, and virulence. During competence, the genes involved in teichoic acid biosynthesis are essential. LytR and ComM play crucial roles in WTA synthesis and immunity against CbpD. Moreover, key virulence factors PspA and PspC become more surface-exposed at midcell during competence, in a CbpD-dependent manner.
Article
Biochemistry & Molecular Biology
Chengxin Li, Kirstine B. Andersen, Pernille L. Elverdal, Ian C. Skovsted, Jens O. Duus, Christian Kjeldsen
Summary: After investigating the capsular polysaccharide of Streptococcus pneumoniae serotype 15F, it was found to have a different phosphorylation substituent and the structure was determined. The locations of previously unassigned O-acetyl groups were identified and full NMR assignments were carried out for 15F and 15A CPS. Lastly, a biosynthetic analysis was performed on serotypes 15F and 15A, leading to a prediction for the structure of the newly discovered serotype 15D.
CARBOHYDRATE RESEARCH
(2021)
Article
Chemistry, Multidisciplinary
Min Liang, Wei Gong, Chongzhen Sun, Jielin Zhao, Hong Wang, Zonggang Chen, Min Xiao, Guofeng Gu
Summary: The biochemical properties and functional identification of three recombinant glycosyltransferases involved in the biosynthesis of GBS serotype VIII CPS were investigated. These enzymes were then used for efficient synthesis of the tetrasaccharide repeating unit in high yield.
CHINESE JOURNAL OF CHEMISTRY
(2022)
Article
Immunology
In Ho Park, K. Aaron Geno, Jigui Yu, Melissa B. Oliver, Kyung-Hyo Kim, Moon H. Nahm
CLINICAL AND VACCINE IMMUNOLOGY
(2015)
Review
Microbiology
K. Aaron Geno, Gwendolyn L. Gilbert, Joon Young Song, Ian C. Skovsted, Keith P. Klugman, Christopher Jones, Helle B. Konradsen, Moon H. Nahm
CLINICAL MICROBIOLOGY REVIEWS
(2015)
Correction
Biochemistry & Molecular Biology
Melissa B. Oliver, Mark P. G. van der Linden, Sharon A. Kuentzel, Jamil S. Saad, Moon H. Nahm
JOURNAL OF BIOLOGICAL CHEMISTRY
(2015)
Article
Biochemical Research Methods
K. Aaron Geno, Brady L. Spencer, Moon H. Nahm
JOURNAL OF IMMUNOLOGICAL METHODS
(2015)
Article
Infectious Diseases
Han Wool Kim, Kyung-Hyo Kim, Jihye Kim, Moon H. Nahm
BMC INFECTIOUS DISEASES
(2016)
Article
Microbiology
Robert L. Burton, K. Aaron Geno, Jamil S. Saad, Moon H. Nahm
JOURNAL OF CLINICAL MICROBIOLOGY
(2016)
Article
Infectious Diseases
Muneki Hotomi, Kouji Nakajima, Masanobu Hiraoka, Moon H. Nahm, Noboru Yamanaka
JOURNAL OF INFECTION AND CHEMOTHERAPY
(2016)
Review
Medicine, General & Internal
Young Keun Kim, Jeon-Soo Shin, Moon H. Nahm
YONSEI MEDICAL JOURNAL
(2016)
Article
Multidisciplinary Sciences
Kimball Aaron Geno, Richard E. Kennedy, Patricia Sawyer, Cynthia J. Brown, Moon H. Nahm
Article
Immunology
Allison M. Brady, Brady L. Spencer, Ann R. Falsey, Moon H. Nahm
CLINICAL AND VACCINE IMMUNOLOGY
(2014)
Article
Immunology
Allison M. Brady, K. Aaron Geno, Alex G. Dalecki, Xiaogang Cheng, Moon H. Nahm
CLINICAL AND VACCINE IMMUNOLOGY
(2014)
Article
Multidisciplinary Sciences
In Ho Park, K. Aaron Geno, Logan K. Sherwood, Moon H. Nahm, Bernard Beall
Article
Multidisciplinary Sciences
Romina Camilli, Brady L. Spencer, Monica Moschioni, Vittoria Pinto, Francesco Berti, Moon H. Nahm, Annalisa Pantosti
Article
Multidisciplinary Sciences
Joon Young Song, Moon H. Nahm, Hee Jin Cheong, Woo Joo Kim
Article
Immunology
Paul V. Licciardi, Fiona M. Russell, Anne Balloch, Robert L. Burton, Moon H. Nahm, Gwendolyn Gilbert, Mimi L. K. Tang, Edward K. Mulholland