期刊
NATURE STRUCTURAL & MOLECULAR BIOLOGY
卷 22, 期 12, 页码 948-952出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/nsmb.3119
关键词
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Single-molecule approaches are having a dramatic impact on views of how proteins work. The ability to observe molecular properties at the single-molecule level allows characterization of subpopulations and acquisition of detailed kinetic information that would otherwise be hidden in the averaging over an ensemble of molecules. In this Perspective, we discuss how such approaches have successfully been applied to in vitro-reconstituted systems of increasing complexity.
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