Article
Microbiology
Etai Boichis, Nadejda Sigal, Ilya Borovok, Anat A. Herskovits
Summary: This study identified a conserved locus containing XRE regulators, a metzincin protease, and a lipoprotein structurally similar to a TIMP-family metzincin inhibitor, which play a crucial role in Listeria monocytogenes life cycle. The XRE regulators act as a regulatory switch that influences the expression of metzincin and TIMP-like genes, affecting Lm morphology and virion release. These proteins also modulate the cell wall structure and may hold promise in combating antibiotic resistant bacterial pathogens.
Article
Food Science & Technology
Qian Guo, Yu Zhang, Xiaowei Fang, Yuying Yang, Xiongyan Liang, Jing Liu, Chun Fang
Summary: Listeria monocytogenes is a ubiquitous foodborne pathogen that forms persistent biofilms in food processing environments, posing a significant threat to human health and food safety. The two-component system virS/virR positively regulates motility and biofilm formation in L. monocytogenes, and its deletion reduces both traits. This study provides valuable insights for controlling biofilm risks of L. monocytogenes in food safety.
Article
Biochemistry & Molecular Biology
Hellen Buijze, Volker Brinkmann, Robert Hurwitz, Anca Dorhoi, Stefan H. E. Kaufmann, Gang Pei
Summary: Human guanylate-binding proteins (hGBPs) are associated with intracellular Mycobacterium tuberculosis (Mtb) and Listeria monocytogenes (Lm) through disruption of phagosomal membranes. The formation of puncta structures by hGBP1 is dependent on GTP-binding and isoprenylation and is required for endolysosomal integrity and repair.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Analytical
Xiaohua Jiang, Zhiwen Lv, Changquan Rao, Xiaowen Chen, Ying Zhang, Feng Lin
Summary: Researchers have developed a simple and highly sensitive nanozyme-based electrochemical sensor for the detection of Listeria monocytogenes (LM). By preparing Pt nanoparticles/hollow carbon spheres as the nanozyme, and using aptamers to regulate its activity, the sensor showed excellent sensing performances for LM.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Environmental Sciences
Jinjin Jing, Ruoyu Zhang, Yunhai Wang, Shaojian Tang, Hanchao Yang, Lidong Du, Bingjie Lin, Lijun Shao, Fengxiang Zhang, Peng Xue
Summary: The study showed that American ginseng saponins and heat-transformed saponins can inhibit Listeria monocytogenes infection, reduce bacterial numbers in mice infected with the pathogen, decrease liver damage, enhance anti-inflammatory ability and immunity, thereby protecting the liver.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2021)
Article
Microbiology
David K. Halladin, Fabian E. Ortega, Katharine M. Ng, Matthew J. Footer, Nenad S. Mitic, Sasa N. Malkov, Ajay Gopinathan, Kerwyn Casey Huang, Julie A. Theriot
Summary: Entropy plays a crucial role in translocating disordered transmembrane proteins through the Gram-positive cell wall, as shown by experimental validation. The study demonstrates that entropic forces are sufficient to drive the translocation of specific proteins to the outer surface.
NATURE MICROBIOLOGY
(2021)
Article
Microbiology
Constanza Melian, Patricia Castellano, Franco Segli, Lucia M. Mendoza, Graciela Margarita Vignolo
Summary: The study showed that treating Listeria monocytogenes with sublethal dose of lactocin AL705 can reduce biofilm formation and induce adaptation mechanisms in the treated cells. This approach targets specific proteins to control L. monocytogenes biofilm, providing valuable data for future research.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Food Science & Technology
Lei Sun, Gil Rogiers, Chris W. Michiels
Summary: This study investigated the antimicrobial mechanisms of trans-cinnamaldehyde (t-CIN) in Listeria monocytogenes, revealing that the YvcK protein plays a crucial role in diverting substrates to UDP-GlcNAc biosynthesis, and t-CIN interferes with this pathway, leading to a peptidoglycan synthesis defect. Complementation with wild-type yvcK gene restored tolerance to t-CIN, and evolutionary experiments identified suppression mutations related to UDP-GlcNAc biosynthesis that partially reversed t-CIN sensitivity. Feeding N-acetylglucosamine or increasing UDP-GlcNAc synthetic enzymes restored t-CIN tolerance in the yvcK mutant.
Article
Immunology
Changyong Cheng, Feng Liu, Haobo Jin, Xiangfei Xu, Jiali Xu, Simin Deng, Jing Xia, Yue Han, Lei Lei, Xian Zhang, Houhui Song
Summary: Research shows that DegU plays a vital role in heat resistance in L. monocytogenes, sensing heat stress through autoregulation and upregulating the production of heat-shock proteins. DegU can also directly regulate the expression of specific operons, thus regulating heat resistance in L. monocytogenes.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Immunology
Cecilie Egholm, Alaz Ozcan, Daniel Breu, Onur Boyman
Summary: Atopic individuals have enhanced type 2 immune cell responses and are more susceptible to bacterial infections. This study shows that neutrophil development, aging, and elimination are accelerated in mice with a predisposition to type 2 immunity, leading to increased susceptibility to bacterial infections. The aging of neutrophils is mediated by IL-4 signaling and contributes to the deficiency in type 2 inflammation and recurrent bacterial infections.
SCIENCE IMMUNOLOGY
(2022)
Article
Plant Sciences
Marketa Samalova, Alesia Melnikava, Kareem Elsayad, Alexis Peaucelle, Evelina Gahurova, Jaromir Gumulec, Ioannis Spyroglou, Elena Zemlyanskaya, Elena Ubogoeva, Darina Balkova, Martin Demko, Nicolas Blavet, Panagiotis Alexiou, Vladimir Benes, Gregory Mouille, Jan Hejatko
Summary: Expansins facilitate cell expansion by mediating pH-dependent cell wall loosening. This study investigates the role of expansins in controlling cell wall biomechanical properties in specific tissues and organs in Arabidopsis. The results show that expansins are spatially specific and play a role in regulating cell wall stiffness and growth arrest. The findings propose that expansins orchestrate cell wall biomechanical properties to control root growth.
Article
Microbiology
Rikke S. S. Thomasen, Patricia T. dos Santos, Eva Sternkopf M. Lillebaek, Marianne N. Skov, Michael Kemp, Birgitte H. Kallipolitis
Summary: Free fatty acids (FFAs) have strong antimicrobial properties against pathogens, but the response of Listeria monocytogenes to FFAs is not fully understood. This study identified mutations in genes involved in wall teichoic acid (WTA) glycosylations that confer tolerance against several FFAs in L. monocytogenes. The FFA tolerant strains lack GlcNAc on their WTAs, resulting in a more hydrophilic surface and reduced binding of FFAs. Importantly, lack of GlcNAc on WTAs also confers tolerance toward acid stress.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Microbiology
Min Lin, Hanhong Dan
Summary: This study explored the binding property of CWBDIspC to design an affinity probe for detecting L. monocytogenes. The results showed that CWBDIspC can effectively detect L. monocytogenes by recognizing the cell wall component lipoteichoic acid. It also demonstrated the ability to separate and capture L. monocytogenes cells, making it a potential antibody alternative for rapid detection and isolation of the pathogen.
MICROBIOLOGY SPECTRUM
(2023)
Article
Developmental Biology
Nadia Rostam, Alexander Goloborodko, Stephan Riemer, Andres Hertel, Dietmar Riedel, Gerd Vorbruggen, Roland Dosch
Summary: The zebrafish germline is specified by germ plasm, and the molecular mechanism of germ plasm localization in vertebrates is largely unknown. This study found that the localization of germ plasm in zebrafish is similar to that in Xenopus and distinct from Drosophila. Non muscle myosin II (NMII) and tight junction (TJ) components, such as ZO2 and claudin-d (Cldn-d), were identified as potential interaction candidates of the germ plasm organizer Bucky ball (Buc). Furthermore, TJ protein ZO1 co-localized with germ plasm, and TJ-like structures were observed at cleavage furrows where the germ plasm is anchored. Injection of the TJ receptor Cldn-d led to the formation of extra germ plasm aggregates, while expression of a dominant-negative version inhibited germ plasm formation. These findings provide the first evidence for the role of TJs in germ plasm localization.
Article
Plant Sciences
Oliver Quinn, Manoj Kumar, Simon Turner
Summary: The plant cell wall is a complex extracellular matrix that plays a crucial role in defense, regulation, and support. Lipid modifications of proteins involved in cell wall metabolism are essential for proper function, including membrane domain localization and targeted delivery. Understanding the contribution of lipid modifications to protein function is important for elucidating cell wall dynamics.
Editorial Material
Immunology
Helene Bierne, Christina Nielsen-LeRoux
Review
Microbiology
Nicolas Personnic, Bianca Striednig, Hubert Hilbi
CURRENT OPINION IN MICROBIOLOGY
(2018)
Review
Immunology
Helene Bierne, Eliane Milohanic, Mounia Kortebi
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2018)
Article
Microbiology
Catherine M. Buckley, Victoria L. Heath, Aurelie Gueho, Cristina Bosmani, Paulina Knobloch, Phumzile Sikakana, Nicolas Personnic, Stephen K. Dove, Robert H. Michell, Roger Meier, Hubert Hilbi, Thierry Soldati, Robert H. Insall, Jason S. King
Article
Multidisciplinary Sciences
Nicolas Personnic, Bianca Striednig, Emmanuelle Lezan, Christian Manske, Amanda Welin, Alexander Schmidt, Hubert Hilbi
NATURE COMMUNICATIONS
(2019)
Article
Cell Biology
Paulina Knobloch, Hendrik Koliwer-Brandl, Fabian M. Arnold, Nabil Hanna, Imre Gonda, Sophia Adenau, Nicolas Personnic, Caroline Barisch, Markus A. Seeger, Thierry Soldati, Hubert Hilbi
CELLULAR MICROBIOLOGY
(2020)
Review
Cell Biology
Helene Bierne, Melanie Hamon
CELLULAR MICROBIOLOGY
(2020)
Article
Multidisciplinary Sciences
Renaud Pourpre, Laurent Naudon, Hamid Meziane, Goran Lakisic, Luc Jouneau, Hugo Varet, Rachel Legendre, Olivia Wendling, Mohammed Selloum, Caroline Proux, Jean-Yves Coppee, Yann Herault, Helene Bierne
Article
Ecology
Nicolas Personnic, Bianca Striednig, Hubert Hilbi
Summary: The study reveals that sessile L. pneumophila in biofilms exhibit phenotypic heterogeneity, adopting growing and nongrowing states, which are controlled by the Legionella quorum sensing system, the transcription factor LvbR, and temperature. This diversity plays a key role in the formation of antibiotic-tolerant, virulent persisters in the biofilm niche, impacting the ecology and virulence of this opportunistic pathogen.
Article
Infectious Diseases
Elias Bodendoerfer, Nicolas Personnic, Carlos A. Mestres, Markus J. Wilhelm, Lilly Meyer, Barbara Hasse
Summary: This study presents the first case of possible prosthetic valve endocarditis caused by Pandoraea spp. in a 37-year-old male injecting drug user with recurrent endocarditis. Biofilm formation and antibiotic resistance of the P. pnomenusa isolates from this patient were also investigated.
INFECTION AND DRUG RESISTANCE
(2021)
Article
Biochemistry & Molecular Biology
Bianca Striednig, Ulrike Lanner, Selina Niggli, Ana Katic, Simone Vormittag, Sabrina Brulisauer, Ramon Hochstrasser, Andres Kaech, Amanda Welin, Antje Flieger, Urs Ziegler, Alexander Schmidt, Hubert Hilbi, Nicolas Personnic
Summary: Legionella pneumophila, the causative agent of Legionnaires' disease, replicates in a distinct compartment within amoebae and macrophages. The pathogen switches between replicative, non-virulent and non-replicating, virulent/transmissive phases. Individual motile and virulent L. pneumophila cells emerge in the cluster of non-growing bacteria within the Legionella-containing vacuole at late stages of infection, with distinct proteomes and phenotypic heterogeneity. The emergence and spreading of a subpopulation of transmissive L. pneumophila at the LCV periphery at the end of an infection cycle are regulated by the Legionella quorum sensing system.
Article
Multidisciplinary Sciences
Jazmin Meza-Torres, Mickael Lelek, Juan J. Quereda, Martin Sachse, Giulia Manina, Dmitry Ershov, Jean-Yves Tinevez, Lilliana Radoshevich, Claire Maudet, Thibault Chaze, Quentin Giai Gianetto, Mariette Matondo, Marc Lecuit, Isabelle Martin-Verstraete, Christophe Zimmer, Helene Bierne, Olivier Dussurget, Pascale Cossart, Javier Pizarro-Cerda
Summary: LLS is a TOMM produced by hypervirulent clones of Listeria monocytogenes, displaying a contact-dependent inhibition mechanism through direct contact with target bacteria. Additionally, interactions between LLS and lipoteichoic acids (LTAs) influence bacterial susceptibility, suggesting a complex mechanism of action for LLS.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Immunology
Natalie Descoeudres, Luc Jouneau, Celine Henry, Kevin Gorrichon, Aurelie Derre-Bobillot, Pascale Serror, Laura Lee Gillespie, Cristel Archambaud, Alessandro Pagliuso, Helene Bierne
Summary: Listeria monocytogenes may enter a persistence phase in hepatocytes, altering their immune functions and creating an environment favorable for persistent infection. This long-term infection results in an overexpression of genes related to antiviral immunity and a decrease in acute phase protein genes, as well as changes in cholesterol metabolism-associated genes in infected cells. These findings suggest that Listeria profoundly deregulates innate immune functions of hepatocytes during persistent infection.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Microbiology
Lei Sun, Gil Rogiers, Pascal Courtin, Marie-Pierre Chapot-Chartier, Helene Bierne, Chris W. Michiels
Summary: AsnB of L. monocytogenes plays a role in mediating the amidation of mDAP residues in peptidoglycan, affecting various cell wall and cell surface-related properties as well as bacterial virulence. This study highlights the importance of amidation of peptidoglycan mDAP residues in cell wall anchoring of invasion proteins and its implications for virulence.
FRONTIERS IN MICROBIOLOGY
(2021)
Review
Immunology
Aurelie Lotoux, Eliane Milohanic, Helene Bierne
Summary: The VBNC state of bacteria, particularly in the case of Lm pathogens, has important implications for environmental, agricultural, and food industry settings, as well as potential risks in the host. The hypothesis that VBNC forms contribute to adaptation, persistence, and transmission of Lm between different ecological niches is put forth.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)