☆ 4.4 Article

DesT Coordinates the Expression of Anaerobic and Aerobic Pathways for Unsaturated Fatty Acid Biosynthesis in Pseudomonas aeruginosa

JOURNAL OF BACTERIOLOGY (2010)

期刊

JOURNAL OF BACTERIOLOGY

卷 192, 期 1, 页码 280-285

出版社

AMER SOC MICROBIOLOGY

DOI: 10.1128/JB.00404-09

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Microbiology

资金

National Institutes of Health [GM 34496, CA21765]
American Lebanese Syrian Associated Charities
NATIONAL CANCER INSTITUTE [P30CA021765] Funding Source: NIH RePORTER
NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [R01GM034496, R37GM034496] Funding Source: NIH RePORTER

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The fabA and fabB genes are responsible for anaerobic unsaturated fatty acid formation in Pseudomonas aeruginosa. Expression of the fabAB operon was repressed by exogenous unsaturated fatty acids, and DNA sequences upstream of the translational start site were used to affinity purify DesT. The single protein interaction with the fabAB promoter detected in wild-type cell extracts was absent in the desT deletion strain, as was the repression of fabAB expression by unsaturated fatty acids. Thus, DesT senses the overall composition of the acyl-coenzyme A pool to coordinate the expression of the operons for the anaerobic (fabAB) and aerobic (desCB) pathways for unsaturated fatty acid synthesis.

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