Article
Biology
Owain J. Bryant, Paraminder Dhillon, Colin Hughes, Gillian M. Fraser
Summary: In addition to the gate recognition motif (GRM), a hydrophobic N-terminal signal is identified on flagellar rod and hook subunits, which are separately and sequentially recognized by the flagellar export machinery. The hydrophobic N-terminal signal may trigger the opening of the flagellar export gate.
Article
Microbiology
Melissa Berni, Luca Bolzoni, Ilaria Menozzi, Alessandra Dodi, Chiara Bracchi, Marina Morganti, Erika Scaltriti, Stefano Pongolini, Martina Tambassi
Summary: This study reveals that a significant portion of the global population of Salmonella Derby is evolving to adapt to the swine host while simultaneously attenuating its virulence for humans. The fixation of deleterious mutations in the SPI-1 genes plays a key role in this process. The study provides insights into the molecular mechanisms underlying the ecology and host adaptation of Salmonella.
MICROBIOLOGY SPECTRUM
(2023)
Article
Microbiology
Jordan J. Hendriksen, Hee Jung Lee, Alexander J. Bradshaw, Keiichi Namba, Fabienne F. V. Chevance, Tohru Minamino, Kelly T. Hughes
Summary: The bacterial flagellum is a highly complex molecular machine consisting of multiple components, including the adaptor protein FIiE. This protein plays a crucial role in connecting different parts of the flagellar machinery, such as the MS-ring, the core T3S secretion complex, and the axial structure, to facilitate flagellum assembly and function. This study combined genetic analysis with structural data to provide insights into the essential role of FIiE in the flagellum.
Article
Multidisciplinary Sciences
Eduardo Soto, Jorge E. Galan, Marfa Lara-Tejero
Summary: In this study, an extensive in vivo cross-linking strategy aided by structure modeling was used to investigate the sorting platform complex of type III secretion systems. The assembly process of this bacterial structure was mapped using identified cross-links as signatures for pairwise intersubunit interactions and systematic genetic deletions. Insights generated by this study could be used for the rational development of antivirulence strategies against medically important bacterial pathogens.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Review
Environmental Sciences
Sajjad Hussain, Ping Ouyang, Yingkun Zhu, Abdul Khalique, Changliang He, Xiaoxia Liang, Gang Shu, Lizi Yin
Summary: The failure of traditional vaccination against Salmonella necessitates the development of novel therapeutics. Understanding the role of T3SS in bacterial pathogenesis and the development of anti-virulence agents is crucial, and the inhibition of T3SS at different stages of infection can help in controlling the disease progression. Insights into T3SS inhibitors of Salmonella can mitigate antibiotic resistance and lead to the development of new therapeutics.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2021)
Review
Microbiology
Tohru Minamino, Miki Kinoshita, Keiichi Namba
Summary: Most motile bacteria use the flagellar type III secretion system (fT3SS) to construct the flagellum. The fT3SS consists of the transmembrane export complex and the cytoplasmic ATPase ring complex, which requires the FliH(12)-FliI(6)-FliJ(1) ring complex as an active protein transporter. The FliI(6) ring hydrolyzes ATP and FliJ activates the export complex. FliH anchors the FliI(6)-FliJ(1) ring to the base of the flagellum. The flagellar cytoplasmic ATPase complex plays multiple roles in efficient flagellar assembly.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Ju-Sim Kim, Lin Liu, Bennett Davenport, Sashi Kant, Thomas E. Morrison, Andres Vazquez-Torres
Summary: Salmonella protects itself from the killing effect of reactive oxygen species (ROS) produced by host cells by activating the transcription of SPI-2 genes.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Immunology
Hsin-Hung Lin, Hsiu-Ling Chen, Chang-Ching Weng, Rajendra Prasad Janapatla, Chyi-Liang Chen, Cheng-Hsun Chiu
Summary: The study revealed that Salmonella induces apoptosis in macrophages through caspase-3 activation, especially via the expression of SPI-1 structure proteins and effectors. This mechanism may help explain why Salmonella infections in children often present as mild bacteremia with less systemic inflammatory response.
JOURNAL OF MICROBIOLOGY IMMUNOLOGY AND INFECTION
(2021)
Article
Veterinary Sciences
Binjie Chen, Xianchen Meng, Jie Ni, Mengping He, Yanfei Chen, Pengpeng Xia, Heng Wang, Siguo Liu, Guoqiang Zhu, Xia Meng
Summary: Research shows that two RyhB paralogs of Salmonella enterica serovar Enteritidis can upregulate Type III secretion system (T3SS) effectors, affecting the bacteria's invasion into intestinal epithelial cells, and thereby impacting its virulence.
VETERINARY RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Nidhi Singh, Thales Kronenberger, Andrea Eipper, Felix Weichel, Mirita Franz-Wachtel, Boris Macek, Samuel Wagner
Summary: The study found that the salt bridges in the assembly of virulence-associated T3SS are not critical, but facilitate the incorporation of the next subunit. The conserved charged residues are crucial for type III-dependent secretion and play a key role in the function of T3SS.
JOURNAL OF MOLECULAR BIOLOGY
(2021)
Review
Immunology
Albane Jouault, Alessandra Mattos Saliba, Lhousseine Touqui
Summary: This mini-review discusses how the type III secretion system (T3SS) of Pseudomonas aeruginosa and the bacterial effectors secreted through this system can activate and manipulate the host immune response, promoting the survival and establishment of the pathogen.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Julie Ming Liang, Lisa Ann Burdette, Han Teng Wong, Danielle Tullman-Ercek
Summary: Protein production using bacteria is costly and inefficient. Researchers have engineered a bacterial secretion system to improve the production process by directly secreting soluble proteins into the culture media. They explored different activation methods and created strains capable of efficiently producing heterologous proteins. They also discovered a genetic relationship between T3SS regulators, hilE and hilD, further enhancing the industrial competitiveness of protein production strains.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Owain J. Bryant, Betty Y. -W. Chung, Gillian M. Fraser
Summary: The activation of the flagellar export gate by FliJ is regulated by flagellar export chaperones, with unladen chaperones preventing the activation of the export gate in the absence of subunit cargo, thus attenuating subunit export. This mechanism couples the availability of subunit cargo to pmf-driven export by the fT3SS.
MOLECULAR MICROBIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Lisa Ann Burdette, Han Teng Wong, Danielle Tullman-Ercek
Summary: In this study, the effects of different components in the growth medium on protein secretion titer were investigated. It was found that the optimal combination of carbon sources, buffers, and salts significantly increased secretion titer. This work demonstrates the importance of optimizing medium composition to enhance protein secretion titer in bacteria.
MICROBIAL CELL FACTORIES
(2021)
Article
Microbiology
Tohru Minamino, Miki Kinoshita, Yumi Inoue, Akio Kitao, Keiichi Namba
Summary: Periodic conformational changes of the GYXLI motif induce a remodeling of hydrophobic side chain interaction networks in FlhA(C) and promote cyclic open-close domain motions of FlhA(C), which are critical for efficient flagellar protein export.
MICROBIOLOGY SPECTRUM
(2022)
Article
Biochemistry & Molecular Biology
Tatsuma Shoji, Akiko Takaya, Yoshiharu Sato, Satoshi Kimura, Tsutomu Suzuki, Tomoko Yamamoto
NUCLEIC ACIDS RESEARCH
(2015)
Article
Infectious Diseases
Naruhiko Ishiwada, Akiko Takaya, Asahi Kimura, Masaharu Watanabe, Moeko Hino, Hidemasa Ochiai, Mari Matsui, Keigo Shibayama, Tomoko Yamamoto
JOURNAL OF INFECTION AND CHEMOTHERAPY
(2016)
Meeting Abstract
Dermatology
Y. Nakamura, Y. Inoue, A. Takaya, H. Takahashi, Y. Kusuya, Y. Katayama, N. Shimojo, H. Matsue
JOURNAL OF INVESTIGATIVE DERMATOLOGY
(2016)
Meeting Abstract
Dermatology
Y. Nakamura, Y. Inoue, A. Takaya, H. Takahashi, Y. Kusuya, Y. Katayama, G. Nunez, N. Shimojo, H. Matsue
JOURNAL OF INVESTIGATIVE DERMATOLOGY
(2016)
Article
Infectious Diseases
Noriko Takeuchi, Misako Ohkusu, Tadashi Hoshino, Sachiko Naito, Akiko Takaya, Tomoko Yamamoto, Naruhiko Ishiwada
JOURNAL OF INFECTION AND CHEMOTHERAPY
(2017)
Meeting Abstract
Dermatology
Y. Nakamura, H. Takahashi, A. Takaya, Y. Katayama, F. Yamade, Y. Kusuya, N. Shimojo, G. Nunez, H. Matsue
JOURNAL OF INVESTIGATIVE DERMATOLOGY
(2017)
Article
Biochemistry & Molecular Biology
Katsutoshi Imamura, Akiko Takaya, Yo-ichi Ishida, Yayoi Fukuoka, Toshiki Taya, Ryo Nakaki, Miho Kakeda, Naoto Imamachi, Aiko Sato, Toshimichi Yamada, Rena Onoguchi-Mizutani, Gen Akizuki, Tanzina Tanu, Kazuyuki Tao, Sotaro Miyao, Yutaka Suzuki, Masami Nagahama, Tomoko Yamamoto, Torben Heick Jensen, Nobuyoshi Akimitsu
Article
Veterinary Sciences
Jun Xu, Kazuasa Suita, Katsuya Okuno, Akiko Takaya, Tomoko Yamamoto, Emiko Isogai
JOURNAL OF VETERINARY MEDICAL SCIENCE
(2018)
Article
Infectious Diseases
Akiko Takaya, Asahi Kimura, Yoshiharu Sato, Naruhiko Ishiwada, Masaharu Watanabe, Mari Matsui, Keigo Shibayama, Tomoko Yamamoto
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2015)
Article
Biochemistry & Molecular Biology
Yoshiharu Sato, Akiko Takaya, Chakib Mouslim, Kelly T. Hughes, Tomoko Yamamoto
JOURNAL OF BIOLOGICAL CHEMISTRY
(2014)
Article
Biochemistry & Molecular Biology
Akiko Takaya, Hikari Takeda, Shogo Tashiro, Hiroto Kawashima, Tomoko Yamamoto
JOURNAL OF BIOLOGICAL CHEMISTRY
(2019)
Article
Multidisciplinary Sciences
Christian Maenne, Akiko Takaya, Yuzuru Yamasaki, Mathias Mursell, Shintaro Hojyo, Tsung-Yen Wu, Jana Sarkander, Mairi A. McGrath, Rebecca Cornelis, Stefanie Hahne, Qingyu Cheng, Tadafumi Kawamoto, Falk Hiepe, Stefan H. E. Kaufmann, Tomoko Yamamoto, Andreas Radbruch, Koji Tokoyoda
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2019)
Review
Immunology
Akiko Takaya, Tomoko Yamamoto, Koji Tokoyoda
FRONTIERS IN IMMUNOLOGY
(2020)
Article
Cell Biology
Yuumi Nakamura, Hiroki Takahashi, Akiko Takaya, Yuzaburo Inoue, Yuki Katayama, Yoko Kusuya, Tatsuma Shoji, Sanami Takada, Seitaro Nakagawa, Rena Oguma, Nobuko Saito, Naoko Ozawa, Taiji Nakano, Fumiya Yamaide, Eishika Dissanayake, Shuichi Suzuki, Amer Villaruz, Saranyaraajan Varadarajan, Masanori Matsumoto, Tomoko Kobayashi, Michihiro Kono, Yasunori Sato, Masashi Akiyama, Michael Otto, Hiroyuki Matsue, Gabriel Nunez, Naoki Shimojo
SCIENCE TRANSLATIONAL MEDICINE
(2020)
Article
Biology
Xiaoning Sun, Kentaro Kawata, Atsuko Miki, Youichiro Wada, Masami Nagahama, Akiko Takaya, Nobuyoshi Akimitsu