Article
Microbiology
Yavuz Ozturk, Crysten E. Blaby-Haas, Noel Daum, Andreea Andrei, Juna Rauch, Fevzi Daldal, Hans-Georg Koch
Summary: Copper is an essential cofactor for redox enzymes, with tightly controlled mechanisms to deliver it for cuproenzyme biogenesis while protecting cells from its toxicity. Many Gram-negative bacteria contain multicopper oxidases for periplasmic copper detoxification, highlighting the importance of copper homeostasis in biological systems.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Plant Sciences
Maria Bernal, Ute Kraemer
Summary: The text discusses the interaction between iron and copper in biological systems and their importance in biological processes. Studies have found an inter-dependence of iron and copper in certain eukaryotes, including green algae, yeast, and mammals. In Arabidopsis, copper deficiency can lead to secondary iron deficiency, affecting root-to-shoot iron translocation and ferroxidase activity.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Microbiology
Yavuz oeztuerk, Andreea Andrei, Crysten E. E. Blaby-Haas, Noel Daum, Fevzi Daldal, Hans-Georg Koch
Summary: Metabolite sensing is crucial for the survival of organisms, and bacteria adjust their metabolism to changing environments through transcriptional regulation. In this study, we demonstrate that the cotranslational export of the protein CutF in Rhodobacter capsulatus regulates the translation of the copper-detoxifying multicopper oxidase CutO in response to extracellular copper. Our findings suggest that the cotranslational export of a protein acting as a sensor is a highly conserved control mechanism for integrating environmental signals into metabolic adaptations.
Article
Environmental Sciences
Wenwei Tang, Haoxiang Liu, Xinping Zeng
Summary: Excessive intake of manganese can seriously affect human health. Manganese oxidizing bacteria, particularly manganese oxidase, play a crucial role in efficient manganese removal. Through site-directed mutagenesis, researchers were able to enhance the enzyme catalytic efficiency for biological manganese removal, providing a potential application of protein engineering in this field.
Article
Biochemistry & Molecular Biology
Noah Hitchcock, David J. Kelly, Andrew Hitchcock, Aidan J. Taylor
Summary: This study found that the cysM gene of Campylobacter jejuni encodes an O-acetyl-L-serine sulfhydrylase with high selectivity for sulfide, enabling it to efficiently utilize sulfide as a sulfur source in locations such as the intestine. However, high concentrations of sulfide inhibit the growth of C. jejuni, and further research is needed to identify the cellular targets responsible for sulfide toxicity.
Article
Electrochemistry
Taiki Adachi, Ievgen Mazurenko, Nicolas Mano, Yuki Kitazumi, Kunishige Kataoka, Kenji Kano, Keisei Sowa, Elisabeth Lojou
Summary: This study investigated the effects of Cu2+ on the bioelectrocatalytic reduction of O2 by CueO, and found that Cu2+ decreased the activity of CueO and caused inactivation and reactivation processes. Kinetic analysis and pseudo-steady-state analysis revealed the relationship between Cu2+ and the enzymatic activity.
ELECTROCHIMICA ACTA
(2022)
Article
Biochemistry & Molecular Biology
Frederic Roulling, Amandine Godin, Georges Feller
Summary: This study reveals the function of methionine-rich regions in copper detoxification process of bacterial Cuproxidases, which can bind toxic Cu(I) ions. The results demonstrate that the length of these methionine-rich loops and the number of Cu(I) ligands in these loops are proportional to the bound Cu(I) ions.
Article
Microbiology
Tomas Richardson-Sanchez, Anson C. K. Chan, Brendil Sabatino, Helen Lin, Erin C. Gaynor, Michael E. P. Murphy
Summary: This study evaluates the impact of iron acquisition-related genes of C. jejuni on bacterial growth under iron-depleted and iron-replete conditions. The disruption of genes like fetA, fetB, fetC, and fetD results in significant growth impairment, and FetE and FetF function redundantly under iron-limited conditions. Furthermore, the study reveals that FetE is an oxidoreductase that can mediate oxidation reactions in bacteria. This research provides crucial insights into the role of the fetMP-fetABCDEF gene cluster in bacterial virulence and is significant for mitigating C. jejuni-related morbidity and mortality.
MICROBIOLOGY SPECTRUM
(2023)
Article
Microbiology
Bahia Khalfaoui-Hassani, Petru-Iulian Trasnea, Stefan Steimle, Hans-Georg Koch, Fevzi Daldal
Summary: CcoA, a member of the bacterial copper importer subfamily CalT in the MFS, plays a crucial role in Cu uptake and cbb3-Cox production through specific residues and periplasm-exposed Cys residues. Elimination of certain Met residues in CcoA completely abolishes its activity, indicating at least one Met residue is required for function. Furthermore, a thiol:disulfide oxidoreduction cycle in CcoA is discovered to be important for its Cu import activity, providing insights into its molecular mechanism.
Article
Microbiology
Nitanshu Garg, Aidan J. Taylor, Federica Pastorelli, Sarah E. Flannery, Phillip J. Jackson, Matthew P. Johnson, David J. Kelly
Summary: The study identified new Cco subunits, a previously uncharacterized set of genes linking copper trafficking and Cco activity, and connections with copper ion homeostasis in this important pathogen, Campylobacter jejuni, causing human food-borne bacterial gastroenteritis.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Immunology
Morgane Nennig, Ann-Katrin Llarena, Malte Herold, Joel Mossong, Christian Penny, Serge Losch, Odile Tresse, Catherine Ragimbeau
Summary: A study on Campylobacter jejuni in Luxembourg revealed an unexpected endemic pattern in the genetic profiles, and tested the hypothesis of stable lineages occurrence by implementing whole genome sequencing (WGS). Results showed that regardless of the typing scheme used, epidemic or endemic signals were detected in the lineages, supporting the clonal expansion of stable genomes in the Campylobacter population.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Biology
Fanis Missirlis
Summary: The article offers a conceptual framework for discussing insects' ability to segregate metal ions to proteins and subcellular compartments. Recent research in Drosophila melanogaster suggests that nuclear iron regulatory proteins and oxidative stress transcription factors play a role in metal-responsive gene expression. Identifying a zinc-regulated chaperone in the endoplasmic reticulum may explain membrane protein trafficking defects seen in zinc transporter mutants.
CURRENT OPINION IN INSECT SCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Stephane L. Benoit, Robert J. Maier
Summary: The nickel chelator DMG inhibits the growth of Campylobacter jejuni, but nickel supplementation can restore growth. The combination of DMG and copper is bactericidal, and this effect is mediated by the proteins SlyD and CueO. Cu-DMG treatment can improve insect survival and reduce C. jejuni colonization in chickens.
Article
Biotechnology & Applied Microbiology
Preenapha Tepkasikul, Pannita Santiyanont, Auttaporn Booncharoen, Mattika Abhisingha, Wuttichai Mhuantong, Kanittha Chantarasakha, Chetsadaporn Pitaksutheepong, Wonnop Visessanguan, Surapun Tepaamorndech
Summary: This study identified a halophilic bacterium, Bacillus piscicola FBU1786, with the ability to degrade histamine and other biogenic amines. Genome sequencing revealed the presence of Cu2+-binding oxidase-encoding genes in this bacterium, and histamine degradation was enhanced through Cu2+ supplementation. The study also evaluated the histamine degradation of the bacterium during fish sauce fermentation.
Article
Biotechnology & Applied Microbiology
Abera Admasie, Adane Eshetu, Tesfaye Sisay Tessema, Jessie Vipham, Jasna Kovac, Ashagrie Zewdu
Summary: A cross-sectional study in Ethiopia found a prevalence of Campylobacter contamination in dairy products, with the highest occurrence in raw milk. Implementing measures such as cleaning cows with warm water and soap, using cloth filters, plastic filters, and aluminum containers for milk, and avoiding concrete floors in collection centers can reduce the likelihood of Campylobacter detection.
Article
Biotechnology & Applied Microbiology
Luca Rossoni, Stephen J. Hall, Graham Eastham, Peter Licence, Gill Stephens
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2015)
Article
Microbiology
Edward Guccione, Andrew Hitchcock, Stephen J. Hall, Francis Mulholland, Neil Shearer, Arnoud H. M. van Vliet, David J. Kelly
ENVIRONMENTAL MICROBIOLOGY
(2010)
Correction
Microbiology
Stephen J. Hall, Andrew Hitchcock, Clive S. Butler, David J. Kelly
JOURNAL OF BACTERIOLOGY
(2009)
Article
Microbiology
Andrew Hitchcock, Stephen J. Hall, Jonathan D. Myers, Francis Mulholland, Michael A. Jones, David J. Kelly
Article
Biochemistry & Molecular Biology
Mohanasundari Pajaniappan, Johanna E. Hall, Shaun A. Cawthraw, Diane G. Newell, Erin C. Gaynor, Joshua A. Fields, Kimberly M. Rathbun, Willie A. Agee, Christopher M. Burns, Stephen J. Hall, David J. Kelly, Stuart A. Thompson
MOLECULAR MICROBIOLOGY
(2008)
Article
Biotechnology & Applied Microbiology
Valentine E. Anyanwu, Stephen J. Hall, Gill Stephens, Anca Pordea
Summary: This study demonstrates the potential of putrescine oxidase from Rhodococcus erythropolis for the bioproduction of N-heterocycles from cadaverine. The enzyme showed good activity under optimal temperature and pH conditions, with complete biotransformation of cadaverine observed in whole cells. A direct assay of enzyme activity for cadaverine oxidation was successfully implemented, confirming the efficiency of the enzyme for this biotechnological application.
JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY
(2021)
Article
Microbiology
MJ Sellars, SJ Hall, DJ Kelly
JOURNAL OF BACTERIOLOGY
(2002)