Article
Biophysics
Ting Wang, Wanwan Yi, Yu Zhang, Hang Wu, Hengwei Fan, Jiulong Zhao, Shige Wang
Summary: In recent years, the healing of chronic wounds has become a challenging problem in clinical practice. This study aimed to address the challenges in wound care by designing a dual-network hydrogel composed of sodium alginate and platelet-rich plasma. The hydrogel showed a three-dimensional network structure and exhibited the potential for cell proliferation and vascular regeneration. When applied to wound skin in rats, the hydrogel effectively promoted wound closure. Overall, this strategy offers a simple and feasible approach for overcoming the shortcomings of conventional wound dressings.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2023)
Article
Chemistry, Multidisciplinary
Shangzhi Li, Qi Dong, Xiaotong Peng, Yun Chen, Hongjun Yang, Weilin Xu, Yanteng Zhao, Pu Xiao, Yingshan Zhou
Summary: A self-healing nanocomposite hydrogel based on hyaluronic acid has been developed, which exhibits good strength, self-healing ability, and the potential to load platelet-rich plasma (PRP) for promoting skin wound healing.
Article
Biotechnology & Applied Microbiology
Meral Nadra, Wanting Niu, Motoichi Kurisawa, Dominique Rousson, Myron Spector
Summary: In implant dentistry, the difficulties of reconstructing large defects in the oral cavity and musculoskeletal system using porous blocks have been addressed by developing a gelatin-based gel incorporating platelet-rich plasma lysate. This gel acts as a scaffold for cell migration and a vehicle for sustained release of therapeutic agents, promoting bone reconstruction.
BIOENGINEERING-BASEL
(2022)
Article
Biochemistry & Molecular Biology
Hang Dong, Jian Li, Xueyuan Huang, Haiting Liu, Rong Gui
Summary: This study developed a novel multifunctional hydrogel, Pltm@CNPs/Gel, which incorporates platelet membrane camouflaged cerium nanoparticles into a gelatin methacryloyl matrix. The Pltm@CNPs/Gel hydrogel possesses anti-oxidative and anti-inflammatory properties and accelerates diabetic wound healing by promoting neovascularization, cell proliferation, and collagen fiber organization. The hydrogel shows potential as a therapeutic option for diabetic wound healing and as a diabetic wound dressing candidate.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Biochemistry & Molecular Biology
Fatemeh Mirjalili, Mahboobeh Mahmoodi
Summary: In this study, platelet-rich fibrin-chitosan (CH-PRF) nanoparticles were loaded into gelatin-chitosan hydrogel (Gel-CH/CH-PRF) to create a hydrogel dressing for wound healing. The Gel-CH/CH-PRF hydrogel exhibited excellent swelling behavior, good porosity, high absorption of wound exudates, and proper vapor permeability rate. It also demonstrated sustained release of proteins and strong antimicrobial activity. In vivo experiments showed that the Gel-CH/CH-PRF hydrogel promoted wound closure, increased epidermis thickness, and enhanced the formation of new granulation tissue. These findings highlight the potential of Gel-CH/CH-PRF hydrogel as an effective wound dressing.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Chemistry, Applied
Ya Zhang, Zi-Li Wang, Ze-Peng Deng, Zi-Lin Wang, Fei Song, Li-Li Zhu
Summary: Diabetes often leads to difficult-to-heal wounds, but platelet rich plasma (PRP) has great potential for diabetic wound treatment. An injectable, self-healing, and non-specific tissue-adhesive hydrogel was designed as an encapsulation and delivery platform for PRP. The hydrogel inhibits PRP enzymolysis and enables sustained release of growth factors, which enhances cell proliferation and migration in vitro, and accelerates healing of full thickness wounds of diabetic skins in vivo.
CARBOHYDRATE POLYMERS
(2023)
Article
Engineering, Biomedical
Jiezhang Tang, Huichen Li, Han Peng, Zhaoxiang Zhang, Chaohua Liu, Yi Cheng, Kai Wang, Zhou Yu, Zhuomin Lyu, Jie Zhang, Chenggang Yi
Summary: This study developed a thermosensitive injectable hydrogel based on decellularized adipose tissue and temperature-controlled platelet-rich plasma, and demonstrated its positive effects on wound healing.
MATERIALS TODAY BIO
(2022)
Article
Medicine, General & Internal
Shaolong Zhou, Li Li, Chen Chen, Yi Chen, Linhua Zhou, Fiona H. Zhou, Jianghui Dong, Liping Wang
Summary: By immobilizing platelets from platelet-rich plasma onto gelatin microspheres, a biomimetic bioreactor GM+PRP was formed which accelerated wound healing in rats by increasing vessel density and reducing inflammation. Compared with PRP alone, GM+PRP significantly increased the expression of angiogenic factors and promoted inflammatory and angiogenic responses.
INTERNATIONAL JOURNAL OF MEDICAL SCIENCES
(2021)
Article
Engineering, Biomedical
Shikun Wei, Pengcheng Xu, Zexin Yao, Xiao Cui, Xiaoxuan Lei, Linlin Li, Yunqing Dong, Weidong Zhu, Rui Guo, Biao Cheng
Summary: This study developed a hydrogel system for the treatment of chronic infected wounds, which can effectively regulate inflammation and enhance collagen deposition and angiogenesis by using antimicrobial peptides and PRP. The hydrogel showed sustained-release capacity, anti-bacterial activity against pathogenic strains, and promoted fibroblast cells proliferation and migration. Additionally, the hydrogel accelerated wound healing in diabetic mouse infection, leading to improved collagen deposition, reduced bacterial load, and balanced inflammatory response.
ACTA BIOMATERIALIA
(2021)
Article
Medicine, General & Internal
Jon Mercader Ruiz, Maider Beitia, Diego Delgado, Pello Sanchez, Jorge Guadilla, Cristina Perez de Arrilucea, Fernando Benito-Lopez, Lourdes Basabe-Desmonts, Mikel Sanchez
Summary: This study developed a new ultrafiltration technique for obtaining Platelet-Rich Plasma (PRP). The technique was able to concentrate growth factors and cytokines present in plasma, resulting in improved effectiveness compared to conventional PRPs.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Dermatology
Shenyu Zhu, Jianfeng Gao, Wenbo Yu, Jianxian Xiong
Summary: A meta-analysis was conducted to evaluate the effect of platelet-rich plasma (PRP) on sternal wound healing (SWH) in cardiac surgery (CS) patients. A total of 1098 relevant studies were reviewed, and 11 studies were included in the analysis, involving 8961 CS patients. The results showed that PRP significantly reduced the incidence of sternal wound infection (SWI), including deep and superficial SWI, compared to the control group. However, caution is needed due to the low sample size of some studies included in the meta-analysis.
INTERNATIONAL WOUND JOURNAL
(2023)
Review
Cell Biology
Edward J. Calabrese, Rachna Kapoor, Gaurav Dhawan, Vittorio Calabrese
Summary: Platelet-rich plasma is widely accepted for wound healing, showing unique hormetic-like concentrations in its complex mixtures, making it a new area for biomedical research and applications.
WOUND REPAIR AND REGENERATION
(2022)
Article
Engineering, Biomedical
Bianza Moise Bakadia, Abeer Ahmed Qaed Ahmed, Lallepak Lamboni, Zhijun Shi, Biampata Mutu Mukole, Ruizhu Zheng, Mazono Pierre Mbang, Bi Zhang, Mario Gauthier, Guang Yang
Summary: The management of diabetic wounds is a challenge, but platelet-rich plasma (PRP) gel, PRP-derived exosomes (PRP-Exos), and mesenchymal stem cell-derived exosomes (MSC-Exos) have potential in wound treatment. However, their limitations include poor mechanical properties, short half-lives of growth factors (GFs), burst release of GFs and exosomes, and protease degradation. To overcome these limitations, dual-crosslinked hydrogels based on silk protein (SP) were developed, which showed improved mechanical properties and sustained release of GFs and exosomes. These hydrogels promoted diabetic wound healing by upregulating GF expression, down-regulating matrix metalloproteinase-9 expression, and promoting angiogenesis and re-epithelialization.
BIOACTIVE MATERIALS
(2023)
Article
Nanoscience & Nanotechnology
Li Mei, Dongjie Zhang, Huarong Shao, Yuanping Hao, Ting Zhang, Weiping Zheng, Yanjing Ji, Peixue Ling, Yun Lu, Qihui Zhou
Summary: Superbacteria-induced skin wound infections are significant health challenges. Probiotic therapy combined with biomimicking hydrogels shows great potential in treating infections and promoting wound repair.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Agriculture, Dairy & Animal Science
Vasileia Angelou, Dimitra Psalla, Chrysostomos Dovas, George M. Kazakos, Christina Marouda, Kyriakos Chatzimisios, Zacharenia Kyrana, Evangelia Moutou, Maria Karayannopoulou, Lysimachos G. Papazoglou
Summary: This study investigated the efficacy of autologous platelet-rich plasma (PRP) infiltration in open wounds in cats. The results showed that PRP infiltration promoted faster and better wound healing, as well as improved perfusion. This is the first study to focus on the topical application of PRP in treating open wounds in laboratory cats, and the results are encouraging, demonstrating faster healing in the PRP group.
Article
Biotechnology & Applied Microbiology
Toshihito Mitsui, Naoki Morimoto, Atsushi Mahara, Sharon Claudia Notodihardjo, Tien Minh Le, Maria Chiara Munisso, Mariko Moriyama, Hiroyuki Moriyama, Natsuko Kakudo, Tetsuji Yamaoka, Kenji Kusumoto
BIOMED RESEARCH INTERNATIONAL
(2020)
Article
Cell & Tissue Engineering
Shuichi Ogino, Michiharu Sakamoto, Sunghee Lee, Hiroki Yamanaka, Itaru Tsuge, Jun Arata, Yuki Sakamoto, Yusuke Kambe, Tetsuji Yamaoka, Naoki Morimoto
JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE
(2020)
Article
Biotechnology & Applied Microbiology
Toshihito Mitsui, Naoki Morimoto, Atsushi Mahara, Sharon Claudia Notodihardjo, Tien Minh Le, Maria Chiara Munisso, Natsuko Kakudo, Tetsuji Yamaoka, Kenji Kusumoto
BIOMED RESEARCH INTERNATIONAL
(2020)
Review
Pediatrics
Motoki Katsube, Shigehito Yamada, Natsuko Utsunomiya, Naoki Morimoto
Summary: The face is a small complex three-dimensional structure composed of various bones and essential organs. Geometric morphometrics (GM) uses shape configurations to retain geometric information and help visualize results, making it advantageous in morphometric studies of congenital facial anomalies.
CONGENITAL ANOMALIES
(2022)
Article
Cell & Tissue Engineering
A. Shoji-Pietraszkiewicz, M. Sakamoto, M. Katsube, S. Ogino, I Tsuge, H. Yamanaka, J. Arata, N. Morimoto
Summary: In this study, cultured epithelial autografts (CEA) were applied after curettage or abrasion of giant congenital melanocytic nevi (GCMN). The results showed successful growth of CEA on wounds and improvement in lesion color. Histopathological examination revealed regenerated dermis without nevus cells.
REGENERATIVE THERAPY
(2021)
Article
Cell & Tissue Engineering
Naoki Morimoto, Toshihito Mitsui, Yasuhiro Katayama, Natsuko Kakudo, Shuichi Ogino, Itaru Tsuge, Michiharu Sakamoto, Masakatsu Hihara, Kenji Kusumoto
Summary: CEA appears to accelerate epithelization after curettage or ablation of CMN, and can significantly improve the color of CMN when applied initially. The color of the target nevus improved significantly in both the Curettage group and non-Curettage group with initial treatment, with no significant differences in epithelization period or incidence of hypertrophic scars among the groups.
REGENERATIVE THERAPY
(2021)
Article
Cell & Tissue Engineering
Yuanjiaozi Li, Eiichi Sawaragi, Michiharu Sakamoto, Takashi Nakano, Hiroki Yamanaka, Itaru Tsuge, Kumiko Matsuno, Yasuhiko Tabata, Naoki Morimoto
Summary: The study concluded that Genocel (R) can serve as an equivalent skin substitute for wound healing, demonstrating comparable performance to conventional substitutes. Additionally, Genocel (R) exhibited the ability to accelerate capillary formation, making it a more suitable treatment option for chronic skin ulcers such as diabetic ulcers.
REGENERATIVE THERAPY
(2022)
Article
Cell & Tissue Engineering
Takashi Nakano, Michiharu Sakamoto, Yasuhiro Katayama, Yoshihiro Shimizu, Masukazu Inoie, Yuanjiaozi Li, Hiroki Yamanaka, Itaru Tsuge, Susumu Saito, Naoki Morimoto
Summary: Autologous cultured epidermis (CE) is an effective approach for treating extensive burns, but its production time is too long. Allogeneic CE can be prepared in advance and used as a wound dressing to promote wound healing. Dried CE, prepared by removing all water and viable cells, accelerates wound healing and may be a new therapeutic strategy. This study evaluated the safety and efficacy of dried CE in wound healing using a miniature swine model.
REGENERATIVE THERAPY
(2023)
Article
Surgery
Shinsuke Akita, Hiraku Kumamaru, Hisashi Motomura, Nobuyuki Mitsukawa, Naoki Morimoto, Minoru Sakuraba
Summary: This study analyzed data from 19,482 free-flap surgeries performed at 407 facilities in Japan and found that free-flap surgeries performed at institutions with a low average annual number of procedures had a higher risk of total necrosis. It is recommended to either aggregate cases into high-volume centers or improve management at low-volume centers.
JOURNAL OF PLASTIC RECONSTRUCTIVE AND AESTHETIC SURGERY
(2023)
Letter
Surgery
Itaru Tsuge, Nobuyuki Mitsukawa, Takane Suzuki, Shinsuke Akita, Yoshimichi Imai, Koichi Omori, Naoki Morimoto
JOURNAL OF PLASTIC RECONSTRUCTIVE AND AESTHETIC SURGERY
(2023)
Article
Surgery
Itaru Tsuge, Maria Chiara Munisso, Tomoko Kosaka, Ayako Takaya, Yoshihiro Sowa, Chang Liu, Goshiro Yamamoto, Susumu Saito, Naoki Morimoto
Summary: This study successfully visualized subcutaneous arteries using photoacoustic tomography and compared the results with intraoperative fluorescence angiography, with a matching rate of 71.3-82.1%. The results of this study demonstrate that photoacoustic tomography can aid in selecting perforators for abdominal flap surgery.
JOURNAL OF PLASTIC RECONSTRUCTIVE AND AESTHETIC SURGERY
(2023)
Article
Cell & Tissue Engineering
Takashi Nakano, Hiroki Yamanaka, Michiharu Sakamoto, Itaru Tsuge, Yasuhiro Katayama, Susumu Saito, Jiro Ono, Tetsuji Yamaoka, Naoki Morimoto
Summary: This study successfully developed a self-assembled, cell-based dermal substitute derived from human dermal fibroblasts through long-term three-dimensional culture. The scaffold-free cultured dermis exhibited superior properties, such as sufficient tensile strength, high biocompatibility, and efficacy in promoting wound healing when compared to the collagen-based artificial dermis.
TISSUE ENGINEERING PART A
(2023)
Article
Cell & Tissue Engineering
Qiannan Zhao, Shuichi Ogino, Sunghee Lee, Yuki Kato, Yuanjiaozi Li, Michiharu Sakamoto, Hiroki Yamanaka, Takashi Nakano, Eiichi Sawaragi, Naoki Morimoto
Summary: Poly-L-lactic acid (PLLA) mesh implants combined with collagen sponge (CS) were replaced with autologous adipose tissue for regeneration. The study compared different combinations of external frames (P (LA/CL), PLLA, LMWPLLA) and internal fillings (PGA nanosheets, CS) and found that PLLA served as a promising external frame material while PGA nanosheets were an effective alternative internal filling material for adipose tissue regeneration.
REGENERATIVE THERAPY
(2023)
Article
Cell & Tissue Engineering
Motoki Katsube, Natsuko Utsunomiya, Yasuhiro Katayama, Hiroki Yamanaka, Itaru Tsuge, Yoshihiro Sowa, Michiharu Sakamoto, Naoki Morimoto
Summary: This study demonstrates that the use of bFGF-CGS in primary palatoplasty can significantly reduce the occurrence of post-operative oronasal fistulas.
REGENERATIVE THERAPY
(2023)
Article
Cell & Tissue Engineering
Tien Minh Le, Naoki Morimoto, Nhung Thi My Ly, Toshihito Mitsui, Sharon Claudia Notodihardjo, Shuichi Ogino, Jun Arata, Natsuko Kakudo, Kenji Kusumoto
Summary: MSCs have become a focus of attention in tissue repair and wound healing. Recent studies have shown the importance of apoptosis in MSC-mediated immunomodulation. High hydrostatic pressure treatment induced complete apoptosis in MSCs, offering potential for manipulated products in cell-based therapies.
STEM CELL REVIEWS AND REPORTS
(2021)
